##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938767_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4293800 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968589128510875 31.0 31.0 34.0 30.0 34.0 2 32.19039522101635 34.0 31.0 34.0 30.0 34.0 3 32.289628534165544 34.0 31.0 34.0 30.0 34.0 4 35.92299198844846 37.0 35.0 37.0 35.0 37.0 5 35.82824840467651 37.0 35.0 37.0 35.0 37.0 6 35.850686571335416 37.0 35.0 37.0 35.0 37.0 7 35.78023545577344 37.0 35.0 37.0 33.0 37.0 8 35.743218827146116 37.0 35.0 37.0 33.0 37.0 9 37.391781871535706 39.0 37.0 39.0 34.0 39.0 10-11 37.313796869905445 39.0 37.0 39.0 34.0 39.0 12-13 37.24468931948391 39.0 37.0 39.0 33.0 39.0 14-15 38.46781137919791 40.0 38.0 41.0 33.5 41.0 16-17 38.39742081606037 40.0 38.0 41.0 33.0 41.0 18-19 38.40769667893241 40.0 38.0 41.0 33.5 41.0 20-21 38.47821102519913 40.0 38.0 41.0 34.0 41.0 22-23 38.43853055568494 40.0 38.0 41.0 34.0 41.0 24-25 38.37357259304113 40.0 38.0 41.0 34.0 41.0 26-27 38.21610950673063 40.0 38.0 41.0 33.0 41.0 28-29 38.06404257301225 40.0 37.5 41.0 33.0 41.0 30-31 37.85821277190368 40.0 37.0 41.0 33.0 41.0 32-33 37.65285819087987 40.0 37.0 41.0 32.0 41.0 34-35 37.63897480087569 40.0 37.0 41.0 32.0 41.0 36-37 37.57527784247054 40.0 37.0 41.0 32.0 41.0 38-39 37.4827671293493 40.0 37.0 41.0 32.0 41.0 40-41 37.33967406493083 40.0 36.5 41.0 31.0 41.0 42-43 37.18440763426336 40.0 36.0 41.0 31.0 41.0 44-45 36.96353486422284 40.0 36.0 41.0 31.0 41.0 46-47 36.72460279938516 39.0 36.0 41.0 30.0 41.0 48-49 36.48265801853836 39.0 35.0 41.0 30.0 41.0 50-51 36.26298104243328 39.0 35.0 41.0 30.0 41.0 52-53 36.06536878755415 39.0 35.0 40.5 29.0 41.0 54-55 35.83124563323862 39.0 35.0 40.0 28.5 41.0 56-57 35.571438352974056 38.0 35.0 40.0 28.0 41.0 58-59 35.269377241604175 38.0 34.0 40.0 28.0 41.0 60-61 34.92317492663841 38.0 34.0 40.0 27.0 41.0 62-63 34.55398376729238 37.0 34.0 40.0 26.0 41.0 64-65 34.19621989845824 36.5 33.0 39.5 26.0 41.0 66-67 33.804233662490105 36.0 33.0 39.0 26.0 40.5 68-69 33.3577958917509 35.5 32.5 39.0 25.0 40.0 70-71 32.88231193814337 35.0 32.0 38.0 24.0 40.0 72-73 32.525613442638225 35.0 32.0 37.5 23.5 40.0 74-75 32.10187141925567 35.0 31.0 37.0 22.5 39.0 76-77 30.05883762634496 33.0 29.0 35.0 20.0 37.5 78-79 31.132325678885834 34.0 30.5 36.0 20.5 38.0 80-81 31.187773068144764 34.0 31.0 36.0 20.5 37.5 82-83 30.87460384740789 34.0 31.0 35.0 20.0 37.0 84-85 30.483417718570962 34.0 30.5 35.0 18.5 36.5 86-87 29.94742896734827 34.0 30.0 35.0 13.5 36.0 88-89 29.42873596814011 34.0 29.5 35.0 7.0 36.0 90-91 29.00801236666822 34.0 29.0 35.0 4.5 35.0 92-93 28.718091550607852 34.0 29.0 35.0 2.0 35.0 94-95 28.318188434486935 34.0 29.0 35.0 2.0 35.0 96-97 27.779729959476455 33.0 28.0 35.0 2.0 35.0 98-99 27.02317399506265 33.0 26.5 35.0 2.0 35.0 100-101 23.82448332479389 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.017127789934608728 1101 2 0.03209893842548439 1101 3 0.12158445487838776 1101 4 0.09086363582475343 1101 5 0.04030742670330767 1101 6 0.11520981330138369 1101 7 0.09483285956878973 1101 8 -0.026401321971810887 1101 9 0.15174016451194916 1101 10-11 0.13401633378325783 1101 12-13 0.18763114767025257 1101 14-15 0.24235582388923405 1101 16-17 0.1765479981368827 1101 18-19 0.06588643658367488 1101 20-21 0.14366466201319383 1101 22-23 0.13663525865033677 1101 24-25 0.08374712154294173 1101 26-27 0.1452722784658178 1101 28-29 0.1629241472613927 1101 30-31 0.16052887187379383 1101 32-33 0.18944802196037358 1101 34-35 0.17225044701379488 1101 36-37 0.1426660010935379 1101 38-39 0.21798144137589048 1101 40-41 0.3089111787169472 1101 42-43 0.18241324105122914 1101 44-45 0.24570906186290387 1101 46-47 0.21169884935429195 1101 48-49 0.224187300156224 1101 50-51 0.37040730070432204 1101 52-53 0.3868260031326898 1101 54-55 0.2856711465747068 1101 56-57 0.2142170760258253 1101 58-59 0.26009028777191645 1101 60-61 0.3210698936685574 1101 62-63 0.29165594299093556 1101 64-65 0.2589703241923189 1101 66-67 0.2818021012288483 1101 68-69 0.25967447144486044 1101 70-71 0.2015375712118299 1101 72-73 0.20795501855103993 1101 74-75 0.3054766734711478 1101 76-77 0.20489985970912272 1101 78-79 0.33673717955162275 1101 80-81 0.3075631605492646 1101 82-83 0.3535060948576181 1101 84-85 0.3603343308202369 1101 86-87 0.39206820825208766 1101 88-89 0.47081159720804067 1101 90-91 0.5300685675413703 1101 92-93 0.3832825074567161 1101 94-95 0.4790494832808676 1101 96-97 0.4488466097349715 1101 98-99 0.4392835480031465 1101 100-101 0.7870677781954498 1102 1 0.036802269750033645 1102 2 0.06696113534878378 1102 3 0.07615543397074731 1102 4 0.0257254724785696 1102 5 0.015273141057640771 1102 6 0.02686032788628978 1102 7 0.07462414344912816 1102 8 0.03973514810780898 1102 9 0.12653559149133287 1102 10-11 0.12418415931675497 1102 12-13 0.13170723658476646 1102 14-15 0.15959670006461124 1102 16-17 0.22330797459927965 1102 18-19 0.15099469756289352 1102 20-21 0.22128123921293508 1102 22-23 0.1578697820738526 1102 24-25 0.09277847699681985 1102 26-27 0.1635898800317861 1102 28-29 0.1936261923911502 1102 30-31 0.0848491634518922 1102 32-33 0.12837790151027662 1102 34-35 0.10010810454162566 1102 36-37 0.14044121695500422 1102 38-39 0.04964438525791337 1102 40-41 0.1767906925933289 1102 42-43 0.11875782680136382 1102 44-45 0.07683856673254752 1102 46-47 -0.03522372873412394 1102 48-49 0.03572817272890205 1102 50-51 0.1349041243283935 1102 52-53 0.13934933058162358 1102 54-55 0.14387340186166142 1102 56-57 0.11512123233473659 1102 58-59 -0.02247140837680206 1102 60-61 -0.11281819462148235 1102 62-63 -0.19881607007483382 1102 64-65 -0.07685024959688036 1102 66-67 -0.09399001127130191 1102 68-69 -0.07763503606675926 1102 70-71 -0.10644407906257669 1102 72-73 -0.06807000816895226 1102 74-75 0.002394027652073305 1102 76-77 -0.0695992456216672 1102 78-79 -0.027468606376249483 1102 80-81 0.04687546615385685 1102 82-83 0.17886600406970743 1102 84-85 0.11678501593270241 1102 86-87 0.15872184517250432 1102 88-89 0.36823741171729196 1102 90-91 0.3634310226577959 1102 92-93 0.31439802186148924 1102 94-95 0.3149261390193594 1102 96-97 0.3835762274890513 1102 98-99 0.46271631571339 1102 100-101 0.7826976493390632 1103 1 -0.01750618298467188 1103 2 0.04324493304803667 1103 3 -0.07998684735556338 1103 4 -0.008130186705280096 1103 5 -0.02301659974084913 1103 6 -0.02986243536064137 1103 7 0.016920180402266283 1103 8 0.027444406985679848 1103 9 0.007805708293822988 1103 10-11 -0.07078583097422353 1103 12-13 -0.02365008490160392 1103 14-15 0.008761576936542781 1103 16-17 -0.05427594961150106 1103 18-19 -0.08384157299522599 1103 20-21 0.02156397774599128 1103 22-23 -0.11260537165235718 1103 24-25 -0.001499083390356759 1103 26-27 0.02198156557711428 1103 28-29 0.012498180387268576 1103 30-31 -0.07961654604235235 1103 32-33 0.020006957969719963 1103 34-35 -0.039310972218508766 1103 36-37 -0.031041027300489077 1103 38-39 0.03787160582927385 1103 40-41 -0.013938808127498703 1103 42-43 0.039873481866237626 1103 44-45 0.05585045293102553 1103 46-47 -0.006793648414017639 1103 48-49 0.08265570810799971 1103 50-51 0.13134164823318173 1103 52-53 -0.010480908613409667 1103 54-55 0.01801974332507683 1103 56-57 0.02146858000604368 1103 58-59 -0.1603099737950231 1103 60-61 -0.052630928152915146 1103 62-63 -0.040339651233445295 1103 64-65 -0.11297267155692481 1103 66-67 -0.07763574959840724 1103 68-69 0.04118722996049229 1103 70-71 -0.15791043582505893 1103 72-73 0.03630993828431173 1103 74-75 0.02489115907695094 1103 76-77 -0.09415763215848472 1103 78-79 -0.08765955050523289 1103 80-81 -0.024758739030176002 1103 82-83 -0.1224860510069341 1103 84-85 -0.14084230467102188 1103 86-87 -0.12449434241760216 1103 88-89 0.13926759795401722 1103 90-91 0.12847338930582808 1103 92-93 0.03757816837947914 1103 94-95 0.047136118718675846 1103 96-97 0.1986243312873377 1103 98-99 0.41565756141053 1103 100-101 0.8273531261109603 1104 1 0.021762750417444465 1104 2 0.04660478856152395 1104 3 0.024970907061913294 1104 4 0.00882886628535573 1104 5 -0.020312569968574223 1104 6 -0.03994489533504719 1104 7 -0.04916585122702344 1104 8 -0.03704965727921916 1104 9 0.042340925869929436 1104 10-11 0.07017498006688783 1104 12-13 -0.050718448933785965 1104 14-15 0.01428820940474651 1104 16-17 0.10263213843338548 1104 18-19 0.06428939887162244 1104 20-21 0.03506479846853239 1104 22-23 -0.01809965552889281 1104 24-25 -0.04386422260359524 1104 26-27 -0.04576853779081347 1104 28-29 -0.010400976784353588 1104 30-31 -0.0752665863623534 1104 32-33 -0.09877559497072497 1104 34-35 0.02377456839503367 1104 36-37 -0.13991793801577046 1104 38-39 0.034878927085223665 1104 40-41 0.032884129049399746 1104 42-43 -0.012263825463612932 1104 44-45 2.3503544177572167E-4 1104 46-47 0.0190239998549373 1104 48-49 -0.11960345619304036 1104 50-51 -0.1143343606566063 1104 52-53 -0.19927009619791392 1104 54-55 -0.16418222357346934 1104 56-57 -0.19355991597830524 1104 58-59 -0.1417940218595959 1104 60-61 -0.14600036382623927 1104 62-63 -0.33500621335070235 1104 64-65 -0.14595125059729241 1104 66-67 -0.24407182886807988 1104 68-69 -0.2454143479447808 1104 70-71 -0.16814624779930654 1104 72-73 -0.22573272192729377 1104 74-75 -0.18029144409350195 1104 76-77 -0.21789657960244213 1104 78-79 -0.21017914701008067 1104 80-81 -0.3012455373037213 1104 82-83 -0.19389961791369004 1104 84-85 -0.013348519043809404 1104 86-87 0.02268738057024322 1104 88-89 0.13583416241495172 1104 90-91 0.21957260776038368 1104 92-93 0.253830008241799 1104 94-95 0.19199079744478809 1104 96-97 0.29980091727252756 1104 98-99 0.2926779957091803 1104 100-101 0.9466814964334702 1105 1 -0.07995598160336215 1105 2 -0.03533178042047069 1105 3 0.07008872461630489 1105 4 -0.015005652732440922 1105 5 3.7530067112356846E-4 1105 6 -0.032144852857641126 1105 7 -0.005415439762927576 1105 8 -0.06429593635577646 1105 9 -0.028660287197766365 1105 10-11 0.045472651079954574 1105 12-13 -0.014811187072034215 1105 14-15 -0.014131980300952307 1105 16-17 -0.011935636786716941 1105 18-19 -0.036425990486470994 1105 20-21 -0.0027416957658203955 1105 22-23 0.02380318914061519 1105 24-25 -0.001746815511467048 1105 26-27 -0.06622704761242204 1105 28-29 0.015571008736685599 1105 30-31 -0.0665638990080879 1105 32-33 -0.052265325685716846 1105 34-35 0.07987322474652814 1105 36-37 0.04042026113685182 1105 38-39 0.0033206760780970512 1105 40-41 -0.0228865295965619 1105 42-43 -0.07645726138770215 1105 44-45 0.09939430338776845 1105 46-47 0.09143148984669835 1105 48-49 0.0633007904598557 1105 50-51 0.09289149262689733 1105 52-53 0.10328902439383825 1105 54-55 0.0658717719646873 1105 56-57 0.07756025672498623 1105 58-59 0.12915586911315557 1105 60-61 -0.04671976048678772 1105 62-63 -0.10352501704497286 1105 64-65 -0.1199182395846563 1105 66-67 -0.06915067254378471 1105 68-69 0.13693216807784125 1105 70-71 -0.005646011654697247 1105 72-73 -0.1634614744941132 1105 74-75 -0.08280854525671089 1105 76-77 -0.09300951397256085 1105 78-79 -0.2131447225646177 1105 80-81 -0.06788528825559581 1105 82-83 -0.027985675306272384 1105 84-85 -0.012625601681826026 1105 86-87 0.09789554562072311 1105 88-89 0.2761945105006909 1105 90-91 0.29295398674185336 1105 92-93 0.14725325208443607 1105 94-95 0.2590838315509387 1105 96-97 0.33805087677015067 1105 98-99 0.42581486626930243 1105 100-101 0.8015983420452741 1106 1 -0.07996830482449369 1106 2 -0.14889783797330125 1106 3 -0.06350765484393861 1106 4 -0.03668027673287355 1106 5 -0.0808779143894327 1106 6 -0.11401095031962427 1106 7 -0.04859453686652415 1106 8 -0.059613790668173294 1106 9 -0.07134068954528061 1106 10-11 -0.08459985242199508 1106 12-13 -0.03188370979367505 1106 14-15 0.026438042163491104 1106 16-17 -0.07213344144444278 1106 18-19 -0.174340076154337 1106 20-21 -0.06779988058926278 1106 22-23 -0.12818780247167894 1106 24-25 -0.1414481639432026 1106 26-27 -0.09376792057132377 1106 28-29 -0.1301862418961406 1106 30-31 -0.03523697073488563 1106 32-33 -0.12260163656554113 1106 34-35 -0.12732930230520623 1106 36-37 -0.21498725874140945 1106 38-39 -0.1398254648882471 1106 40-41 -0.14224715792255438 1106 42-43 -0.10529619914865407 1106 44-45 -0.014226643374790626 1106 46-47 0.11532265432326483 1106 48-49 0.14240897364397398 1106 50-51 0.14640468469164603 1106 52-53 0.08586675961190338 1106 54-55 0.11351950647050302 1106 56-57 0.010080389363544384 1106 58-59 0.0020031210913771247 1106 60-61 0.010394073289923256 1106 62-63 -0.10362716411187733 1106 64-65 -0.0938077082050981 1106 66-67 0.034883543049275545 1106 68-69 -0.04947707139852042 1106 70-71 -0.09003017281077064 1106 72-73 -0.0116491695882317 1106 74-75 0.15654990106774846 1106 76-77 0.010726400529158298 1106 78-79 0.09641707951992728 1106 80-81 0.04130042947028301 1106 82-83 0.26792742450390605 1106 84-85 0.10266553724383698 1106 86-87 0.12205889500439326 1106 88-89 0.30671709421662996 1106 90-91 0.3085769481814786 1106 92-93 0.4759037237020216 1106 94-95 0.47278584023598214 1106 96-97 0.3826060480572693 1106 98-99 0.3579732162691336 1106 100-101 0.9699144925875558 1107 1 -0.1251508863999291 1107 2 -0.10159595559344581 1107 3 -0.1215568272247296 1107 4 -0.05723872250667483 1107 5 -0.03390116773355345 1107 6 -0.050731064328424225 1107 7 0.018932137206320476 1107 8 0.006718322143193234 1107 9 -0.06347238289948365 1107 10-11 -0.06802047494350916 1107 12-13 -0.08317422979178701 1107 14-15 -0.12184763299151768 1107 16-17 -0.06280462379901053 1107 18-19 -0.02048090649721246 1107 20-21 -0.07245328192489353 1107 22-23 -0.15126795843946894 1107 24-25 -0.07679466525491563 1107 26-27 -0.13094225106718937 1107 28-29 -0.055946494181114304 1107 30-31 -0.15354040832332316 1107 32-33 0.011166545977758346 1107 34-35 -0.1146506131763303 1107 36-37 0.05294061887569512 1107 38-39 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9071.0 13 11958.0 14 14837.0 15 18748.0 16 23268.0 17 29538.0 18 28724.0 19 22611.0 20 21897.0 21 23581.0 22 25762.0 23 29019.0 24 34063.0 25 41303.0 26 50421.0 27 62146.0 28 76681.0 29 93463.0 30 113756.0 31 138254.0 32 170404.0 33 213584.0 34 280604.0 35 388428.0 36 586606.0 37 899682.0 38 781982.0 39 87738.0 40 11.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.39102228718189 19.482102505046143 14.588432659439125 23.538442548332846 2 15.80485816759048 20.737249056779543 39.37106991476082 24.086822860869162 3 19.903814802738832 23.952652661977737 29.76272765382645 26.38080488145698 4 13.351157482882295 16.76806092505473 36.629302715543346 33.251478876519634 5 13.770250128091668 37.409008337603055 34.44277795891751 14.377963575387767 6 32.571032651730405 36.213540453677396 16.94901951651218 14.266407378080023 7 28.596767432111413 32.35439470864968 20.635497694349993 18.413340164888908 8 26.04830220317667 35.68196003539988 19.394359308770788 18.87537845265266 9 25.813731426708276 17.022963342493828 20.20329312031301 36.96001211048488 10-11 25.17213191112767 26.475802319623643 27.247228562112813 21.10483720713587 12-13 25.975790674926643 23.571498439610604 27.73427965904327 22.71843122641949 14-15 22.592273742534708 25.31121423945974 26.061565939526805 26.03494607847875 16-17 22.23979445264831 28.411187397182363 26.676846682464205 22.672171467705116 18-19 22.10318855835986 27.36213228872395 28.602726262812105 21.931952890104082 20-21 23.01376403185989 26.34541664725884 28.53049746145605 22.110321859425216 22-23 22.51462573943826 26.327856444175325 28.49817178257022 22.6593460338162 24-25 22.56887838278448 26.390341888304064 28.692335460431316 22.348444268480133 26-27 22.409959583529158 26.507280041846442 28.6875313533905 22.395229021233902 28-29 22.149180213330848 27.172364805067772 28.46923471051283 22.209220271088544 30-31 22.477607247659414 26.572464949462017 28.378708835996086 22.57121896688248 32-33 22.283047650100144 26.659509059574273 28.42186408309656 22.63557920722903 34-35 22.795847538100453 26.676150874366154 28.297794339427963 22.23020724810543 36-37 22.832913332838736 26.38213782949909 28.50116717704579 22.283781660616384 38-39 22.464382230332998 26.746946278847815 28.44643228358424 22.342239207234947 40-41 22.671049298788564 26.716432250194337 28.299210718621627 22.313307732395472 42-43 22.46040960440464 26.780202414958815 27.997976083313635 22.761411897322912 44-45 22.65448451981559 26.72659922700971 28.073579639077117 22.545336614097582 46-47 22.884309684262032 27.00055161771181 27.666056203521666 22.449082494504495 48-49 22.720901486388215 26.950956583304176 27.724490966329924 22.603650963977692 50-51 22.550270391123906 27.157131944347405 27.813732888038047 22.478864776490646 52-53 22.845952303321067 27.16113931715497 27.406970515627183 22.58593786389678 54-55 22.51042200381946 26.91501700125763 27.650717313335505 22.923843681587407 56-57 22.67283059294797 27.106583911686617 27.460000465787882 22.76058502957753 58-59 22.84117093865414 26.91179765363248 27.478733220519143 22.76829818719424 60-61 22.585102852385138 27.015912571105844 27.847337133408246 22.551647443100766 62-63 22.465322092319155 26.98630583632214 27.840595742698778 22.70777632865993 64-65 22.281813312217615 27.16009129442452 27.625925753411895 22.93216963994597 66-67 22.400873362445413 27.074887918486173 27.387609898107712 23.1366288209607 68-69 22.455635416767343 26.908262520440964 27.5914386162179 23.044663446573797 70-71 22.564649159212745 26.786937835170203 27.374331406330278 23.274081599286774 72-73 22.45250128091667 26.778774046299315 27.572942382039216 23.195782290744795 74-75 22.28029950160697 27.292444920583165 27.18483627556011 23.242419302249758 76-77 22.40127059198714 26.83201255858864 27.389341890977686 23.37737495844653 78-79 22.470996667644 26.91460783864906 27.38101187274866 23.23338362095828 80-81 22.585174573844746 26.880356998546045 27.23172416780571 23.302744259803497 82-83 22.524290837952396 26.80964413805953 27.410359122455635 23.25570590153244 84-85 22.417776794447807 26.903162699706552 27.269795984908473 23.409264520937164 86-87 22.410333522723562 26.824210415343035 27.252764117735417 23.51269194419799 88-89 22.37951295247808 26.81192460932868 27.02499840828417 23.783564029909073 90-91 22.359339521789114 26.736090716790862 27.16577171686151 23.738798044558514 92-93 22.477746984023476 26.744433834831614 27.1537449345568 23.624074246588105 94-95 22.2863366450083 26.573472582205177 27.29529258356062 23.844898189225905 96-97 22.168210229657905 26.33488126308646 27.554675061096823 23.942233446158816 98-99 22.165734952773068 26.494626716097752 27.099581849552706 24.24005648157647 100-101 22.238818605592495 26.06528965722109 27.49029495850212 24.205596778684296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1143.0 1 766.0 2 483.5 3 588.5 4 517.0 5 530.0 6 946.0 7 1983.0 8 3018.0 9 3369.0 10 2927.0 11 2463.0 12 2558.5 13 2692.5 14 2901.0 15 3183.5 16 3465.0 17 3828.0 18 4184.0 19 4719.0 20 5511.0 21 6561.0 22 8081.5 23 10231.5 24 13024.0 25 16568.0 26 22122.5 27 28791.0 28 34661.5 29 41423.0 30 49267.5 31 57415.0 32 66926.0 33 73594.5 34 78661.0 35 85613.0 36 94717.5 37 103461.5 38 109545.5 39 114741.5 40 120898.0 41 127615.0 42 132243.5 43 136897.0 44 143263.5 45 150674.0 46 155776.5 47 165249.0 48 184119.5 49 184158.0 50 167863.5 51 160478.5 52 157115.0 53 154780.0 54 152113.5 55 143023.0 56 129464.0 57 116049.0 58 105436.0 59 94358.0 60 79985.0 61 66226.5 62 53112.5 63 40282.5 64 28920.5 65 20696.0 66 15005.0 67 11455.0 68 8409.0 69 5882.0 70 4348.0 71 3067.0 72 2314.5 73 1908.0 74 1413.5 75 957.0 76 638.0 77 405.0 78 242.0 79 130.5 80 69.5 81 44.5 82 34.5 83 23.5 84 15.5 85 14.0 86 5.5 87 1.5 88 2.0 89 2.0 90 2.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37901159811821694 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.144068191345661E-4 16-17 0.002177558339931995 18-19 2.328939400996786E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.3973636405980718E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008081419721458849 36-37 0.017455400810470912 38-39 0.011004238669709815 40-41 0.007860170478364152 42-43 0.0033769621314453397 44-45 0.001979598490847268 46-47 0.001525455307652895 48-49 5.822348502491965E-5 50-51 4.657878801993572E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.2605151613955004E-4 60-61 5.822348502491965E-4 62-63 0.0 64-65 0.0 66-67 0.001164469700498393 68-69 7.336159113139876E-4 70-71 0.0020028878848572357 72-73 0.0 74-75 0.0 76-77 0.09655782756532674 78-79 0.03473613116586706 80-81 2.328939400996786E-5 82-83 0.0 84-85 0.0 86-87 0.1480273883273557 88-89 0.3220340956728306 90-91 0.27720201220364243 92-93 0.0 94-95 2.328939400996786E-5 96-97 1.164469700498393E-5 98-99 2.445386371046626E-4 100-101 6.171689412641483E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4293800.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.70340234185076 #Duplication Level Percentage of deduplicated Percentage of total 1 66.58281527876797 30.430611957386606 2 16.17048818801577 14.780926554420601 3 6.660298101707977 9.131948515770736 4 3.418155914043544 6.248854200268349 5 1.963454490786523 4.486827528616509 6 1.2840742499989557 3.5211937250707517 7 0.8382039481497138 2.681614060076993 8 0.574745671436772 2.1014266132729564 9 0.4203795221794025 1.729149699459619 >10 1.9365115230195307 15.202397519380314 >50 0.10433228160433553 3.2757059052250845 >100 0.041853295348216926 3.3819288673058745 >500 0.0029551852128424875 0.9598484790663147 >1k 0.0015285438780311344 1.2750551521103315 >5k 2.0380585040412572E-4 0.7925112225690414 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9964 0.23205552191531975 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9928 0.23121710373096094 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8448 0.19674880059620847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5693 0.13258652009874702 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4562 0.10624621547347338 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6302575806977502E-4 2 0.0 0.0 0.0 0.0 1.6302575806977502E-4 3 0.0 0.0 0.0 0.0 1.6302575806977502E-4 4 0.0 0.0 0.0 6.986818202990358E-5 1.6302575806977502E-4 5 0.0 0.0 0.0 6.986818202990358E-5 1.863151520797429E-4 6 0.0 0.0 0.0 9.315757603987145E-5 1.863151520797429E-4 7 0.0 0.0 0.0 9.315757603987145E-5 1.863151520797429E-4 8 0.0 0.0 0.0 1.164469700498393E-4 1.863151520797429E-4 9 0.0 0.0 0.0 1.863151520797429E-4 2.0960454608971076E-4 10-11 0.0 0.0 0.0 2.2124924309469468E-4 2.678280311146304E-4 12-13 0.0 0.0 0.0 2.5618333410964647E-4 9.548651544086823E-4 14-15 2.328939400996786E-5 0.0 0.0 3.027621221295822E-4 0.0012110484885183288 16-17 2.328939400996786E-5 0.0 0.0 3.493409101495179E-4 0.0014439424286180073 18-19 3.493409101495179E-5 0.0 0.0 3.842750011644697E-4 0.0016069681866877825 20-21 4.657878801993572E-5 0.0 0.0 4.5414318319437325E-4 0.0017467045507475894 22-23 4.657878801993572E-5 0.0 0.0 6.288136382691322E-4 0.0019330197028273324 24-25 4.657878801993572E-5 0.0 0.0 9.315757603987145E-4 0.002002887884857236 26-27 4.657878801993572E-5 0.0 0.0 0.0014788765196329591 0.0022940053099818345 28-29 1.164469700498393E-4 0.0 0.0 0.003935907587684568 0.002468675765056593 30-31 1.3973636405980715E-4 0.0 0.0 0.012471470492337789 0.0025036098560715448 32-33 1.3973636405980715E-4 0.0 0.0 0.02939121524057944 0.002654990917136336 34-35 1.3973636405980715E-4 0.0 0.0 0.05547533653174344 0.0028645954632260467 36-37 1.6302575806977502E-4 0.0 0.0 0.09880525408728866 0.0028762401602310307 38-39 1.6302575806977502E-4 0.0 0.0 0.16188457776328657 0.0029810424332758862 40-41 1.6302575806977502E-4 0.0 0.0 0.23169453630816528 0.003004331827285854 42-43 1.6302575806977502E-4 0.0 0.0 0.3054753365317434 0.0030509106153057894 44-45 1.863151520797429E-4 0.0 0.0 0.3965368671107178 0.0031324234943406774 46-47 2.0960454608971073E-4 0.0 0.0 0.5106432530625553 0.0032139363733755645 48-49 2.328939400996786E-4 0.0 0.0 0.6339140155573152 0.0032721598584004846 50-51 2.328939400996786E-4 0.0 0.0 0.7647654758023197 0.0033769621314453397 52-53 2.328939400996786E-4 0.0 0.0 0.8932996413433323 0.003726303041594858 54-55 2.328939400996786E-4 0.0 0.0 1.037752107690158 0.003796171223624761 56-57 2.328939400996786E-4 0.0 0.0 1.210151846848945 0.0038776841026596487 58-59 2.328939400996786E-4 0.0 0.0 1.4017886254599654 0.003947552284689552 60-61 2.328939400996786E-4 0.0 0.0 1.6140365177698075 0.004005775769714472 62-63 2.328939400996786E-4 0.0 0.0 1.8192975918766594 0.004285248497834087 64-65 2.328939400996786E-4 0.0 0.0 2.034910801620942 0.004448274255903861 66-67 2.328939400996786E-4 0.0 0.0 2.285667706926266 0.004541431831943733 68-69 2.328939400996786E-4 0.0 0.0 2.5736876426475384 0.004611300013973636 70-71 2.328939400996786E-4 0.0 0.0 2.8821673110065675 0.004681168196003539 72-73 2.328939400996786E-4 0.0 0.0 3.1972611672644278 0.0051236666821929296 74-75 2.328939400996786E-4 0.0 0.0 3.5220783455214493 0.005519586380362382 76-77 2.328939400996786E-4 0.0 0.0 3.867588150356328 0.00565932274442219 78-79 2.328939400996786E-4 0.0 0.0 4.271717359914295 0.005810703805486981 80-81 2.328939400996786E-4 0.0 0.0 4.702512925613675 0.0059271507755368205 82-83 2.328939400996786E-4 0.0 0.0 5.136114863291257 0.006346359867716242 84-85 2.328939400996786E-4 0.0 0.0 5.575993292654525 0.006381293958731194 86-87 2.328939400996786E-4 0.0 0.0 6.035097116773022 0.00663747729284084 88-89 2.328939400996786E-4 0.0 0.0 6.539184405421771 0.0068936606269504866 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15320 0.0 22.230259 1 TACATGG 15050 0.0 22.103577 2 ACATGGG 15095 0.0 20.874495 3 GAGTACT 12570 0.0 20.557074 12-13 GTATCAA 25515 0.0 20.516315 1 AGAGTAC 20855 0.0 18.795809 10-11 GGACCGT 455 0.0 18.773365 6 GTACTTT 13730 0.0 18.543873 14-15 AGTACTT 13570 0.0 18.517605 12-13 CATGGGG 8470 0.0 18.152758 4 CAACGCA 28970 0.0 17.871346 5 TACCTGG 2790 0.0 17.85937 2 TCAACGC 29035 0.0 17.814993 4 ATCAACG 29055 0.0 17.802732 3 TATCAAC 29485 0.0 17.655764 2 GGTATCA 20100 0.0 17.559793 1 AACGCAG 29695 0.0 17.498943 6 GTGTAGC 2910 0.0 17.02306 1 CGCGGAA 270 0.0 16.796745 82-83 TACTTTT 15790 0.0 15.929237 14-15 >>END_MODULE