FastQCFastQC Report
Thu 26 May 2016
SRR938766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305073
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99020.23000771415490517No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87340.20287693147131303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76070.17669851359082644No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50900.1182326060440787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49300.11451606047098387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG149450.023.9027042
GTACATG153400.023.8596461
ACATGGG151250.022.4577163
GTATCAA229000.022.1684021
GAGTACT114300.019.95355412-13
CAACGCA262750.019.1566965
TCAACGC262650.019.1459274
GTATAGC16900.019.1258531
ATCAACG263550.019.0625463
TATCAAC263600.019.0409322
AACGCAG267500.018.8513416
AGAGTAC194200.018.62273410-11
CATGGGG81850.018.4892794
GTACTTT121500.018.47834614-15
AGTACTT121050.018.27234312-13
GGTATCA175150.018.2643221
GTGTAGC29000.017.8659921
TAGCCCT30800.017.2510414
ACGCAGA291500.017.1369177
CGCAGAG292050.016.9259648
Produced by FastQC (version 0.11.5)