FastQCFastQC Report
Thu 26 May 2016
SRR938766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305073
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99640.2314478755644794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99140.23028645507288725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84960.1973485699313345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56160.1304507496156279No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43890.10194949075195704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG144000.022.5122092
GTACATG150700.022.0057031
ACATGGG147650.020.8948753
GAGTACT121950.019.8235312-13
GTATCAA250950.019.6421171
GTACTTT130350.018.38226114-15
AGAGTAC202300.018.21227610-11
GGTATCA201700.018.210721
AGTACTT129850.017.97801612-13
CATGGGG78850.017.8176294
AACGTCG1650.001475350717.2540456
TCAACGC282400.017.244184
ATCAACG283700.017.1651613
CAACGCA284000.017.147035
TATCAAC285750.017.1084582
GTATAGC16500.017.0131131
AACGCAG290300.016.8186846
TACCTGG29550.016.0621032
CATGGGA77150.015.5648344
ACGCAGA316550.015.34833057
Produced by FastQC (version 0.11.5)