##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938766_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4305073 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02934909582253 33.0 31.0 34.0 30.0 34.0 2 32.233944465053206 34.0 31.0 34.0 30.0 34.0 3 32.3257352430493 34.0 31.0 34.0 30.0 34.0 4 35.94659974406938 37.0 35.0 37.0 35.0 37.0 5 35.84617124959321 37.0 35.0 37.0 35.0 37.0 6 35.866234788585466 37.0 35.0 37.0 35.0 37.0 7 35.78159023087414 37.0 35.0 37.0 33.0 37.0 8 35.76372851284984 37.0 35.0 37.0 33.0 37.0 9 37.40570601241837 39.0 37.0 39.0 34.0 39.0 10-11 37.32992390605223 39.0 37.0 39.0 34.0 39.0 12-13 37.2514603120551 39.0 37.0 39.0 33.0 39.0 14-15 38.471364248643404 40.0 38.0 41.0 33.5 41.0 16-17 38.396898031694235 40.0 38.0 41.0 33.0 41.0 18-19 38.53041609282816 40.0 38.0 41.0 34.0 41.0 20-21 38.52510921417593 40.0 38.0 41.0 34.0 41.0 22-23 38.451887110857356 40.0 38.0 41.0 34.0 41.0 24-25 38.381964603155396 40.0 38.0 41.0 34.0 41.0 26-27 38.208960452006274 40.0 38.0 41.0 33.0 41.0 28-29 38.04270787045887 40.0 37.0 41.0 33.0 41.0 30-31 37.83871527846334 40.0 37.0 41.0 33.0 41.0 32-33 37.70864082908699 40.0 37.0 41.0 32.5 41.0 34-35 37.69768085233398 40.0 37.0 41.0 32.5 41.0 36-37 37.60008215888558 40.0 37.0 41.0 32.0 41.0 38-39 37.48538770422708 40.0 37.0 41.0 32.0 41.0 40-41 37.328798373453836 40.0 36.5 41.0 31.0 41.0 42-43 37.14536269187538 40.0 36.0 41.0 31.0 41.0 44-45 36.929264381811876 40.0 36.0 41.0 31.0 41.0 46-47 36.67507403474924 39.0 36.0 41.0 30.0 41.0 48-49 36.40751515711813 39.0 35.0 41.0 30.0 41.0 50-51 36.191828919045044 39.0 35.0 41.0 29.5 41.0 52-53 35.97932474083483 39.0 35.0 40.0 29.0 41.0 54-55 35.70901747775241 38.5 35.0 40.0 28.0 41.0 56-57 35.43090035871633 38.0 34.5 40.0 28.0 41.0 58-59 35.11980319497486 38.0 34.0 40.0 27.0 41.0 60-61 34.75282103230305 37.5 34.0 40.0 26.5 41.0 62-63 34.39100939751776 37.0 33.5 40.0 26.0 41.0 64-65 33.991377381986325 36.5 33.0 39.0 26.0 41.0 66-67 33.578726539596424 36.0 33.0 39.0 25.5 40.0 68-69 33.22655469489135 35.5 32.0 39.0 24.5 40.0 70-71 32.79217355896172 35.0 32.0 38.0 23.5 40.0 72-73 32.29936786205484 35.0 31.5 37.5 22.0 39.5 74-75 31.804510515849557 35.0 31.0 37.0 21.5 39.0 76-77 29.869880835934723 33.0 28.5 35.0 19.5 37.5 78-79 30.830401598300426 34.0 30.5 36.0 19.5 38.0 80-81 30.890130202205633 34.0 31.0 35.5 20.0 37.0 82-83 30.559168915370307 34.0 30.5 35.0 18.0 37.0 84-85 30.14772955069519 34.0 30.0 35.0 15.5 36.5 86-87 29.558517010048377 34.0 29.5 35.0 7.5 36.0 88-89 28.93818327819296 34.0 29.0 35.0 2.0 35.5 90-91 28.402922319784125 33.5 28.5 35.0 2.0 35.0 92-93 28.093140697033476 33.0 27.5 35.0 2.0 35.0 94-95 27.727624943874353 33.0 27.0 35.0 2.0 35.0 96-97 27.079586687612498 33.0 26.5 35.0 2.0 35.0 98-99 26.19576055969318 33.0 25.0 34.5 2.0 35.0 100-101 22.454464767496393 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1160568864795124 1101 2 0.17449512382823684 1101 3 0.09446420192346494 1101 4 0.07400784610782551 1101 5 -0.06129507751374064 1101 6 0.10031441382754736 1101 7 0.1660002698195271 1101 8 0.16851830837303083 1101 9 0.24474575322213354 1101 10-11 0.23247391658315308 1101 12-13 0.19101513686789673 1101 14-15 0.33438087918582227 1101 16-17 0.36077784349474484 1101 18-19 0.36293762102430094 1101 20-21 0.24063960059365996 1101 22-23 0.19051508679204687 1101 24-25 0.2165510715283574 1101 26-27 0.1465577708704231 1101 28-29 0.2192804086985447 1101 30-31 0.2686445709703662 1101 32-33 0.3326462754938575 1101 34-35 0.43436664762052857 1101 36-37 0.45446299075946683 1101 38-39 0.4671257096570969 1101 40-41 0.4929217531902381 1101 42-43 0.5343270257917467 1101 44-45 0.4646454330410137 1101 46-47 0.5448554431481583 1101 48-49 0.38749833029163483 1101 50-51 0.38711160585892657 1101 52-53 0.24557832112270006 1101 54-55 0.2791724341938888 1101 56-57 0.22787386123627584 1101 58-59 0.2490004630793763 1101 60-61 0.40484681642066533 1101 62-63 0.08013686172029111 1101 64-65 0.1253991611786418 1101 66-67 0.1995354028698415 1101 68-69 0.15526715780799094 1101 70-71 0.2472763783886549 1101 72-73 0.06193842552712425 1101 74-75 0.04129994912713997 1101 76-77 0.06923778470849129 1101 78-79 -0.08255686564697484 1101 80-81 0.16619881706563788 1101 82-83 0.3051314471724105 1101 84-85 0.3880124814700743 1101 86-87 0.43075559007712627 1101 88-89 0.40373304726091064 1101 90-91 0.3009836226690652 1101 92-93 0.2997314218488576 1101 94-95 0.487974550492833 1101 96-97 0.47272924215584666 1101 98-99 0.33072432223102766 1101 100-101 1.0349373848526966 1102 1 0.24228101293247306 1102 2 0.16468395699039462 1102 3 0.17472144237324017 1102 4 0.09410700476401246 1102 5 -0.043212484209583124 1102 6 -0.03486680472592241 1102 7 0.17495669362994448 1102 8 0.0805850322378987 1102 9 0.18513199370833178 1102 10-11 0.20994246044698883 1102 12-13 0.27882645006126694 1102 14-15 0.25012517558050007 1102 16-17 0.2617274781461276 1102 18-19 0.3115297955478198 1102 20-21 0.3238385983367351 1102 22-23 0.22166378167160872 1102 24-25 0.28947143351437177 1102 26-27 0.25057364298286444 1102 28-29 0.2638981005426331 1102 30-31 0.272451429787516 1102 32-33 0.2843115541591317 1102 34-35 0.2523072738328551 1102 36-37 0.25552492042260866 1102 38-39 0.28847511655520464 1102 40-41 0.2834631539284942 1102 42-43 0.27420348902682434 1102 44-45 0.28382916236035527 1102 46-47 0.24015141602092882 1102 48-49 0.2633540896769091 1102 50-51 0.24073107056524634 1102 52-53 0.17503295074129 1102 54-55 0.1467971429725594 1102 56-57 0.18438777943556062 1102 58-59 0.25073503785809237 1102 60-61 0.2540637809200845 1102 62-63 0.1688911600786298 1102 64-65 0.23960718117210433 1102 66-67 0.35635571536897714 1102 68-69 0.27001479420516716 1102 70-71 0.26092166471030964 1102 72-73 0.2588552246358873 1102 74-75 0.09722463595247888 1102 76-77 0.14662391169675004 1102 78-79 0.05402116273463875 1102 80-81 0.27150888863170763 1102 82-83 0.21120622638328612 1102 84-85 0.3024401906930514 1102 86-87 0.3796700196519929 1102 88-89 0.3397375602238988 1102 90-91 0.37499040158099106 1102 92-93 0.4990897631172082 1102 94-95 0.5902109331633127 1102 96-97 0.667772629219332 1102 98-99 0.7360137492023462 1102 100-101 1.3666746650924608 1103 1 0.17131737212194764 1103 2 0.13237800880112616 1103 3 0.12546686959409925 1103 4 0.047044797124229376 1103 5 0.04939292698290387 1103 6 0.026213311010650386 1103 7 0.018162399855320643 1103 8 0.060548791492287535 1103 9 0.10852265694781948 1103 10-11 0.12582635931131136 1103 12-13 0.15572097503216042 1103 14-15 0.2173362225473383 1103 16-17 0.08695644397737112 1103 18-19 0.025452871642613673 1103 20-21 0.09478066544364339 1103 22-23 0.1682923232584841 1103 24-25 0.18090417355162458 1103 26-27 0.21704633859597777 1103 28-29 0.055837803386978635 1103 30-31 0.20255609109189976 1103 32-33 0.3310203529999214 1103 34-35 0.2728345951402886 1103 36-37 0.3257261750046183 1103 38-39 0.25880242924854713 1103 40-41 0.44340082476838916 1103 42-43 0.44632145757613273 1103 44-45 0.4987995821592506 1103 46-47 0.5053264591298827 1103 48-49 0.4890657911614582 1103 50-51 0.5336496657902643 1103 52-53 0.41738197711735836 1103 54-55 0.41732215693201624 1103 56-57 0.2917688702573926 1103 58-59 0.4001388960515584 1103 60-61 0.4553758543491355 1103 62-63 0.3966156381180568 1103 64-65 0.4536798503276316 1103 66-67 0.44134630138921693 1103 68-69 0.31579507776285 1103 70-71 0.29038188372377505 1103 72-73 0.3913752562619308 1103 74-75 0.48956743248899315 1103 76-77 0.4572050634325677 1103 78-79 0.359183507738539 1103 80-81 0.5630528812974198 1103 82-83 0.5661824837725682 1103 84-85 0.6280298719252322 1103 86-87 0.6646123051878448 1103 88-89 0.5186431696944886 1103 90-91 0.5082145588990556 1103 92-93 0.5724272804415484 1103 94-95 0.6040773254093921 1103 96-97 0.6490971469451452 1103 98-99 0.9918276892759046 1103 100-101 1.5085906022526103 1104 1 0.1069879084774712 1104 2 0.045852359610329074 1104 3 0.06983049387357454 1104 4 0.038764037227551285 1104 5 -0.21767271060592464 1104 6 -0.05078556041075899 1104 7 0.07345920182881827 1104 8 -0.06487087649008316 1104 9 -0.00600161394459775 1104 10-11 0.008635910222210441 1104 12-13 -0.030811320447661217 1104 14-15 -0.004138453478333304 1104 16-17 0.11260345698667606 1104 18-19 0.14140819180338582 1104 20-21 0.1090023527363897 1104 22-23 0.07976964166799405 1104 24-25 0.056494193019439365 1104 26-27 0.003461065740012259 1104 28-29 0.0642246296678266 1104 30-31 -0.08878995665684641 1104 32-33 -0.03716970118346552 1104 34-35 -0.0911692648695066 1104 36-37 -0.004403469573787788 1104 38-39 -0.06729766063767784 1104 40-41 -0.04657328051528964 1104 42-43 -0.09590207777212356 1104 44-45 -0.004587847449784022 1104 46-47 -0.01698216484954429 1104 48-49 -0.02207190510571877 1104 50-51 -0.1509797261646284 1104 52-53 -0.20955560971189158 1104 54-55 -0.16918348886465395 1104 56-57 -0.30215650224130286 1104 58-59 -0.09254502386517771 1104 60-61 0.0026617002700888293 1104 62-63 -0.17692716301529288 1104 64-65 -0.2283558757342803 1104 66-67 -0.15092588737572044 1104 68-69 -0.04968250004551322 1104 70-71 -0.11787125858359815 1104 72-73 -0.015118151974427008 1104 74-75 -0.02697464852216669 1104 76-77 -0.02818947447934761 1104 78-79 -0.1576316841295018 1104 80-81 0.0024836524540745586 1104 82-83 -0.030031260882172006 1104 84-85 -0.0413778243483911 1104 86-87 0.09400992233191374 1104 88-89 0.08109215297358574 1104 90-91 0.14163647177846173 1104 92-93 0.162059908429395 1104 94-95 0.17341844587047817 1104 96-97 0.1730740378627651 1104 98-99 0.5756849018222887 1104 100-101 1.3321634891359935 1105 1 0.08434411078467008 1105 2 0.08230622929350773 1105 3 0.0886239832808684 1105 4 0.03570019561832538 1105 5 -0.060125914660169144 1105 6 -0.004824479275995941 1105 7 0.0729797792854896 1105 8 0.09604366247387475 1105 9 0.048654598765018875 1105 10-11 0.03954907895852244 1105 12-13 0.1055906000081066 1105 14-15 0.16075524545436082 1105 16-17 0.13065070658464606 1105 18-19 0.10940559779901804 1105 20-21 -0.023574794066213656 1105 22-23 0.014914896799041344 1105 24-25 0.05490884590714984 1105 26-27 0.12431160941554253 1105 28-29 0.15083880970128405 1105 30-31 0.12809806777343624 1105 32-33 0.07758105094895029 1105 34-35 0.18773193197407068 1105 36-37 0.19141840460223136 1105 38-39 0.1958872449847533 1105 40-41 0.15032324812608522 1105 42-43 0.18925353263444578 1105 44-45 0.15968869906112104 1105 46-47 0.0964625497644036 1105 48-49 0.11078078002194047 1105 50-51 0.03525212578290393 1105 52-53 0.12643788233528852 1105 54-55 0.2325695382856523 1105 56-57 0.20048645005875443 1105 58-59 0.21710529152288416 1105 60-61 0.19753950016215782 1105 62-63 0.11876238676749296 1105 64-65 0.05442242111674034 1105 66-67 0.10889811065316479 1105 68-69 0.21357217404929685 1105 70-71 0.19239330220658957 1105 72-73 0.2600256040772777 1105 74-75 0.2555705367775509 1105 76-77 0.24524613252050287 1105 78-79 0.06700763329599013 1105 80-81 0.14107424445841943 1105 82-83 0.3838660374123073 1105 84-85 0.3197539239835301 1105 86-87 0.37346408145997145 1105 88-89 0.32871185383026 1105 90-91 0.3668705497173903 1105 92-93 0.4246761381474293 1105 94-95 0.5413259856738613 1105 96-97 0.8385394768557397 1105 98-99 1.0536849697051274 1105 100-101 1.581182644981478 1106 1 0.194576597071773 1106 2 0.1031243125719783 1106 3 0.0588976172212341 1106 4 0.04482889764108222 1106 5 -0.042393393844641025 1106 6 0.02784104871990678 1106 7 0.05780082740481873 1106 8 0.08259235957569189 1106 9 0.07136627208416257 1106 10-11 0.11908106276749919 1106 12-13 0.13175117713924323 1106 14-15 0.1170722849512984 1106 16-17 0.16570340207820067 1106 18-19 0.12068618806629416 1106 20-21 0.1004209530547513 1106 22-23 0.14614012904746687 1106 24-25 0.11096254860768084 1106 26-27 0.06865385824426085 1106 28-29 0.19146845984904104 1106 30-31 0.11152519054270016 1106 32-33 0.14477561888580226 1106 34-35 0.18836096316780981 1106 36-37 0.08325409430882047 1106 38-39 0.19084640169316458 1106 40-41 0.33812427845432325 1106 42-43 0.27698839626761895 1106 44-45 0.3138688382081227 1106 46-47 0.3200738804081027 1106 48-49 0.31712551898959873 1106 50-51 0.35414710361053636 1106 52-53 0.20424556769794577 1106 54-55 0.2698575017786169 1106 56-57 0.242343982191926 1106 58-59 0.3022217239333713 1106 60-61 0.24528024130076176 1106 62-63 0.23955512362354625 1106 64-65 0.20835610403121052 1106 66-67 0.11972087956694111 1106 68-69 0.11367898198070492 1106 70-71 0.2426679950186923 1106 72-73 0.2676085579815748 1106 74-75 0.40221015361944623 1106 76-77 0.35455778404837446 1106 78-79 0.37417115634128706 1106 80-81 0.26065557066713296 1106 82-83 0.3588378136210437 1106 84-85 0.4293078371795751 1106 86-87 0.518248063953294 1106 88-89 0.45769417067230833 1106 90-91 0.3770153338021913 1106 92-93 0.5582790489704799 1106 94-95 0.7029804037010763 1106 96-97 0.7247507417163881 1106 98-99 0.8084949073442331 1106 100-101 1.2962404916046744 1107 1 0.0205012795282542 1107 2 0.003806857420578069 1107 3 -0.0024609324165538737 1107 4 -0.021900463860575314 1107 5 -0.0907197063325853 1107 6 -0.0010577938231506323 1107 7 0.009805627900895786 1107 8 0.005495591099702324 1107 9 -0.017818920255493254 1107 10-11 -0.04808082630785293 1107 12-13 0.04575039777788703 1107 14-15 0.06550491835785266 1107 16-17 0.10116580816759324 1107 18-19 0.060073426113923745 1107 20-21 0.06042592103388955 1107 22-23 0.046469623713569774 1107 24-25 -0.02772935217350181 1107 26-27 -0.012448092959090218 1107 28-29 0.08975240066381218 1107 30-31 0.003220933416137939 1107 32-33 0.03985302971070581 1107 34-35 0.15570710929780773 1107 36-37 0.10029693426668018 1107 38-39 0.017537113652863923 1107 40-41 -0.12905772400090143 1107 42-43 -0.027490054544891507 1107 44-45 -0.014485350448190104 1107 46-47 -0.12911890004446747 1107 48-49 0.04887825234141019 1107 50-51 -0.06651635022411995 1107 52-53 -0.11745705174226373 1107 54-55 -0.04797471769817463 1107 56-57 -0.10551743636391109 1107 58-59 0.0536972060315577 1107 60-61 0.02044697299983511 1107 62-63 -0.034780152290672106 1107 64-65 -0.079210873138436 1107 66-67 0.003668912552882375 1107 68-69 -0.03684942450787787 1107 70-71 -0.11676087746838704 1107 72-73 -0.08605029186703206 1107 74-75 0.008822523579251396 1107 76-77 -0.07430810132180454 1107 78-79 -0.10050848735134466 1107 80-81 -0.03549740507273924 1107 82-83 -0.03241836328011516 1107 84-85 -0.05587772095130461 1107 86-87 0.0093314404631748 1107 88-89 0.02864965382882545 1107 90-91 0.15253385699204358 1107 92-93 0.34592778972648475 1107 94-95 0.497626006204694 1107 96-97 0.7062957780912917 1107 98-99 0.9326529003834132 1107 100-101 1.7652054694067445 1108 1 0.15332278173565328 1108 2 0.10501223918860347 1108 3 0.048977152408276936 1108 4 0.0515601658314111 1108 5 0.007237669464423391 1108 6 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-1.1718103703777913 2316 96-97 -1.0946012646055046 2316 98-99 -1.0190705544715009 2316 100-101 -1.207539068218324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 18.0 4 34.0 5 120.0 6 205.0 7 575.0 8 1369.0 9 2673.0 10 4398.0 11 6657.0 12 9188.0 13 12423.0 14 15193.0 15 19691.0 16 24184.0 17 31008.0 18 29794.0 19 24328.0 20 23844.0 21 25525.0 22 27936.0 23 31919.0 24 36921.0 25 44162.0 26 53300.0 27 66069.0 28 80211.0 29 97085.0 30 118406.0 31 144478.0 32 175842.0 33 221345.0 34 290582.0 35 402594.0 36 600349.0 37 894851.0 38 718506.0 39 69283.0 40 4.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.38786627702978 19.506237457621634 14.582442265187003 23.523454000161585 2 15.814923463551025 20.714793918709393 39.37315348659593 24.097129131143653 3 19.885539687712612 23.9338334100258 29.714455480778142 26.46617142148345 4 13.332805274149823 16.726243666483704 36.698378866049424 33.24257219331705 5 13.782268137816175 37.39384135823581 34.42444311439575 14.399447389552261 6 32.550346405570366 36.21250079850404 16.953001504091244 14.284151291834352 7 28.634195053138473 32.322007083271295 20.630451562609974 18.413346300980262 8 26.009942223976225 35.69767574208382 19.4048045178328 18.88757751610716 9 25.852987858742466 16.966216368456468 20.209692146915977 36.971103625885085 10-11 25.15858616102445 26.476856490006096 27.2487946197428 21.11576272922666 12-13 25.963636388976447 23.564687520978158 27.715325617010443 22.756350473034953 14-15 22.60339139429227 25.30485545773556 26.058141174377297 26.033611973594873 16-17 22.282331335400258 28.37238025351752 26.688887603546522 22.656400807535697 18-19 22.12876529619823 27.377514852825957 28.572024214223546 21.92169563675227 20-21 23.05509105188228 26.33400176954026 28.510213415661013 22.10069376291645 22-23 22.507945858293226 26.335128347417104 28.463222342571193 22.693703451718473 24-25 22.590692976324025 26.36642388649358 28.675845554319157 22.367037582863237 26-27 22.417896566037797 26.48813816963285 28.692433287874852 22.401531976454503 28-29 22.174908532329184 27.138610657705453 28.46156151126822 22.22491929869714 30-31 22.490303881025945 26.59043179987889 28.374350446554566 22.544913872540604 32-33 22.288750968915043 26.660256399833404 28.405615886188222 22.645376745063324 34-35 22.818521683388575 26.70480487801762 28.241154632354743 22.235518806239064 36-37 22.84041725231452 26.359717495092173 28.49092197428183 22.308943278311475 38-39 22.469340697366064 26.705080501904334 28.449883732829555 22.37569506790005 40-41 22.71155638905789 26.690728587434233 28.29633563289844 22.301379390609437 42-43 22.45779091052695 26.744812718943685 27.99138627937446 22.806010091154906 44-45 22.685914216006815 26.704795828223883 28.03155406279639 22.577735892972917 46-47 22.912179454049102 26.985179640457762 27.662208925200186 22.440431980292946 48-49 22.744455694920536 26.91987671088731 27.73936510613349 22.59630248805866 50-51 22.561176379724152 27.12551298497296 27.799938328564732 22.513372306738155 52-53 22.870982675555098 27.118471626381247 27.39279914649531 22.617746551568345 54-55 22.51107007941561 26.925803581031033 27.645535859670673 22.917590479882687 56-57 22.68185590203071 27.119463370981222 27.433027353515577 22.765653373472496 58-59 22.850452386576993 26.876566326531798 27.480518840981667 22.792462445909543 60-61 22.584231778928668 27.003658436162848 27.827750247010353 22.58435953789813 62-63 22.493883924174597 26.990906953882977 27.784707626953285 22.730501494989138 64-65 22.268194498967482 27.20151637278427 27.593373766843747 22.936915361404502 66-67 22.427822389609673 27.03142119137963 27.412828253345445 23.127928165665256 68-69 22.487412498397237 26.890285487045023 27.58136696690223 23.040935047655505 70-71 22.559718582776995 26.762498702110793 27.372384680656538 23.305398034455678 72-73 22.47317292877496 26.785329772572965 27.55432950846594 23.187167790186137 74-75 22.335933533005626 27.272038864088582 27.194885360323006 23.19714224258279 76-77 22.425931278059725 26.795801969603318 27.375447744083353 23.402819008253612 78-79 22.4749086240923 26.920185018549653 27.38805804374902 23.216848313609024 80-81 22.583197310499305 26.855065357161855 27.23423747577547 23.327499856563367 82-83 22.548663550400004 26.818456812699615 27.398497274534563 23.234382362365825 84-85 22.441106109002103 26.88150700348171 27.250687735144098 23.426699152372095 86-87 22.405996855080843 26.828915816734337 27.26521667097809 23.49987065720672 88-89 22.389424551726194 26.761976423375973 27.054493087665023 23.79410593723281 90-91 22.353171067035206 26.72050540919399 27.14177301054183 23.78455051322897 92-93 22.497441379364812 26.700803288414036 27.153027581070994 23.648727751150158 94-95 22.299091696958534 26.57437374290399 27.255014505020952 23.871520055116523 96-97 22.174194864914494 26.33175840715231 27.53634987774297 23.957696850190224 98-99 22.186127854277967 26.501513447042598 27.00980912518789 24.302549573491554 100-101 22.356557329449505 25.863664132421842 27.552816124394024 24.226962413734633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1119.0 1 757.5 2 488.5 3 611.0 4 533.5 5 517.0 6 957.0 7 2005.0 8 3025.0 9 3304.5 10 2910.0 11 2477.5 12 2513.5 13 2685.0 14 2912.5 15 3206.0 16 3456.0 17 3778.0 18 4207.0 19 4739.5 20 5466.0 21 6580.0 22 8176.0 23 10285.5 24 13046.0 25 16637.0 26 21950.5 27 28441.5 28 34541.5 29 41411.5 30 49384.5 31 57484.0 32 66734.5 33 73472.0 34 78837.5 35 85505.5 36 94641.0 37 104055.0 38 110045.5 39 114848.0 40 121142.5 41 128099.5 42 132196.5 43 136983.0 44 143855.0 45 150845.0 46 155916.5 47 165812.5 48 184229.0 49 183721.0 50 167409.0 51 160952.0 52 158349.0 53 155280.5 54 152695.5 55 143926.5 56 130252.5 57 116850.5 58 105855.0 59 94495.0 60 80208.0 61 66605.0 62 53540.5 63 40830.0 64 29362.0 65 20905.5 66 15095.0 67 11526.5 68 8579.0 69 6069.0 70 4571.5 71 3253.0 72 2333.5 73 1884.0 74 1440.0 75 1011.5 76 697.0 77 404.5 78 238.5 79 151.5 80 98.0 81 71.0 82 48.5 83 32.5 84 24.5 85 17.5 86 10.5 87 6.0 88 3.0 89 1.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23539670523589265 2 0.0 3 0.0 4 0.0 5 0.026410701978805008 6 0.003437804655112701 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002880322819148479 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.903551228980322E-4 26-27 0.0010685068522647584 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0067710814659821105 36-37 0.01679414030842218 38-39 0.012717554382933807 40-41 0.00803702980181753 42-43 0.007270492277366725 44-45 0.00334489101578533 46-47 3.6004035239355986E-4 48-49 0.0016259886882289802 50-51 1.1614204915921287E-5 52-53 0.0 54-55 0.0 56-57 0.00117303469650805 58-59 3.716545573094812E-4 60-61 0.0021253994996135953 62-63 5.807102457960644E-4 64-65 0.001463389819406082 66-67 0.005191549597416815 68-69 2.0905568848658313E-4 70-71 1.3937045899105544E-4 72-73 0.0 74-75 4.4133978680500885E-4 76-77 0.05880271948930947 78-79 0.13789545496673344 80-81 8.362227539463325E-4 82-83 6.620096802075133E-4 84-85 0.0 86-87 0.10419103230072987 88-89 0.349843080477381 90-91 0.38530124808568866 92-93 4.4133978680500885E-4 94-95 2.787409179821109E-4 96-97 8.1299434411449E-5 98-99 0.0 100-101 9.988216227692307E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4305073.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.46183281040228 #Duplication Level Percentage of deduplicated Percentage of total 1 66.49546409583589 30.230056713749992 2 16.027018042105638 14.572352293590148 3 6.762506727612608 9.223078506898352 4 3.4756376078941873 6.320354233585284 5 2.016735329318229 4.584224218214845 6 1.2458293842404387 3.398261230597514 7 0.8437823449540123 2.685192432626794 8 0.6027736714036286 2.19225566974913 9 0.43148449082556445 1.7654468203974027 >10 1.9438579882514568 15.16562514566113 >50 0.10670519232094358 3.337158680893372 >100 0.0433946956504816 3.470346623599478 >500 0.0031216619096235427 1.0153308916476274 >1k 0.001484068564928151 1.2495394586234176 >5k 2.0469911240386773E-4 0.7907770801654886 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9964 0.2314478755644794 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9914 0.23028645507288725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8496 0.1973485699313345 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5616 0.1304507496156279 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4389 0.10194949075195704 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3937045899105544E-4 2 2.3228409831842574E-5 0.0 0.0 0.0 1.3937045899105544E-4 3 2.3228409831842574E-5 0.0 0.0 0.0 1.3937045899105544E-4 4 2.3228409831842574E-5 0.0 0.0 1.1614204915921286E-4 1.3937045899105544E-4 5 2.3228409831842574E-5 0.0 0.0 1.1614204915921286E-4 1.62598868822898E-4 6 2.3228409831842574E-5 0.0 0.0 1.1614204915921286E-4 2.3228409831842572E-4 7 2.3228409831842574E-5 0.0 0.0 1.1614204915921286E-4 2.555125081502683E-4 8 2.3228409831842574E-5 0.0 0.0 1.1614204915921286E-4 2.555125081502683E-4 9 2.3228409831842574E-5 0.0 0.0 2.787409179821109E-4 2.555125081502683E-4 10-11 2.3228409831842574E-5 0.0 0.0 2.903551228980322E-4 3.600403523935599E-4 12-13 2.3228409831842574E-5 0.0 0.0 3.0196932781395347E-4 9.755932129373881E-4 14-15 2.3228409831842574E-5 0.0 0.0 3.0196932781395347E-4 0.0012891767456672627 16-17 2.3228409831842574E-5 0.0 0.0 3.1358353272987474E-4 0.0015911460734812162 18-19 2.3228409831842574E-5 0.0 0.0 3.484261474776386E-4 0.0017653591472200356 20-21 2.3228409831842574E-5 0.0 0.0 3.948829671413238E-4 0.0020441000652021467 22-23 2.3228409831842574E-5 0.0 0.0 5.226392212164579E-4 0.002229927343856887 24-25 4.645681966368515E-5 0.0 0.0 6.155528605438281E-4 0.0022763841635205723 26-27 6.968522949552772E-5 0.0 0.0 0.0011846489014239712 0.0025435108765867616 28-29 9.29136393273703E-5 0.0 0.0 0.003437804655112701 0.0026248103109982107 30-31 9.29136393273703E-5 0.0 0.0 0.011056723079957064 0.0026480387208300533 32-33 9.29136393273703E-5 0.0 0.0 0.027049483249180675 0.0029035512289803216 34-35 9.29136393273703E-5 0.0 0.0 0.05263557667895527 0.0030893785076350623 36-37 9.29136393273703E-5 0.0 0.0 0.09539907917937746 0.0030893785076350623 38-39 1.1614204915921286E-4 0.0 0.0 0.1584525976679141 0.003205520556794275 40-41 1.1614204915921286E-4 0.0 0.0 0.22595435663924862 0.0032519773764579604 42-43 1.1614204915921286E-4 0.0 0.0 0.2946291503070912 0.0033332768108694094 44-45 1.1614204915921286E-4 0.0 0.0 0.38267643777469046 0.0035423324993559924 46-47 1.1614204915921286E-4 0.0 0.0 0.49076984292716985 0.0037513881878425753 48-49 1.1614204915921286E-4 0.0 0.0 0.6037179857345044 0.003972058081245081 50-51 1.1614204915921286E-4 0.0 0.0 0.7284661607364149 0.004111428540236136 52-53 1.1614204915921286E-4 0.0 0.0 0.8530865794842504 0.004343712638554561 54-55 1.1614204915921286E-4 0.0 0.0 0.9927822362129515 0.004413397868050089 56-57 1.1614204915921286E-4 0.0 0.0 1.1652764076242144 0.0045063115073774584 58-59 1.1614204915921286E-4 0.0 0.0 1.352299949385295 0.004622453556536671 60-61 1.1614204915921286E-4 0.0 0.0 1.5543638865124936 0.004761824015527728 62-63 1.1614204915921286E-4 0.0 0.0 1.7590526339506902 0.005063793343341681 64-65 1.1614204915921286E-4 0.0 0.0 1.975843382911277 0.005226392212164579 66-67 1.1614204915921286E-4 0.0 0.0 2.224213155038254 0.005330920056407871 68-69 1.1614204915921286E-4 0.0 0.0 2.5077158970358924 0.005388991080987477 70-71 1.3937045899105544E-4 0.0 0.0 2.8114621982019816 0.005470290515398926 72-73 1.509846639069767E-4 0.0 0.0 3.1207136324982176 0.005795488253044722 74-75 1.858272786547406E-4 0.0 0.0 3.4379788681864394 0.006097457580858675 76-77 1.858272786547406E-4 0.0 0.0 3.784546742877531 0.006271670654597494 78-79 1.858272786547406E-4 0.0 0.0 4.181311211215234 0.006387812703756707 80-81 2.0905568848658316E-4 0.0 0.0 4.607715130498368 0.006550411572579606 82-83 2.0905568848658316E-4 0.0 0.0 5.038753117542955 0.006980137154468693 84-85 2.2066989340250443E-4 0.0 0.0 5.472206859210053 0.007073050793796064 86-87 2.4389830323434702E-4 0.0 0.0 5.925753175381695 0.007398248531441859 88-89 2.555125081502683E-4 0.0 0.0 6.417905108693859 0.007700217859255813 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 14400 0.0 22.512209 2 GTACATG 15070 0.0 22.005703 1 ACATGGG 14765 0.0 20.894875 3 GAGTACT 12195 0.0 19.82353 12-13 GTATCAA 25095 0.0 19.642117 1 GTACTTT 13035 0.0 18.382261 14-15 AGAGTAC 20230 0.0 18.212276 10-11 GGTATCA 20170 0.0 18.21072 1 AGTACTT 12985 0.0 17.978016 12-13 CATGGGG 7885 0.0 17.817629 4 AACGTCG 165 0.0014753507 17.254045 6 TCAACGC 28240 0.0 17.24418 4 ATCAACG 28370 0.0 17.165161 3 CAACGCA 28400 0.0 17.14703 5 TATCAAC 28575 0.0 17.108458 2 GTATAGC 1650 0.0 17.013113 1 AACGCAG 29030 0.0 16.818684 6 TACCTGG 2955 0.0 16.062103 2 CATGGGA 7715 0.0 15.564834 4 ACGCAGA 31655 0.0 15.3483305 7 >>END_MODULE