FastQCFastQC Report
Thu 26 May 2016
SRR938765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5295921
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120150.22687271958928393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91460.17269895075851774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57790.10912171839421321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA221650.028.1254521
GGTATCA155350.027.4035361
GTACATG184100.026.6776471
TACATGG190950.024.8635832
ACATGGG191600.023.7900413
ATCAACG262350.023.6233943
TCAACGC263450.023.560734
CAACGCA265100.023.3964335
TATCAAC267600.023.1599332
AACGCAG269850.022.949256
ACGCAGA297550.020.5749197
GAGTACT130000.020.21395512-13
CGCAGAG301000.020.1658738
CATGGGA96150.019.7610824
AGAGTAC230300.019.29478510-11
CATGGGG97400.019.069654
AGTACTT140150.018.59798412-13
GCAGAGT315550.018.530989
GTACTTT146600.018.03818714-15
GAGTACA155300.017.0029891
Produced by FastQC (version 0.11.5)