##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938765_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5295921 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56350160812444 33.0 31.0 34.0 30.0 34.0 2 31.630981089030595 33.0 31.0 34.0 30.0 34.0 3 31.65390911231493 33.0 31.0 34.0 28.0 34.0 4 35.207871869689896 37.0 35.0 37.0 33.0 37.0 5 35.239097222182885 37.0 35.0 37.0 33.0 37.0 6 35.317407113890106 37.0 35.0 37.0 33.0 37.0 7 35.22382433574821 37.0 35.0 37.0 32.0 37.0 8 35.1937951868995 37.0 35.0 37.0 32.0 37.0 9 36.75745918415324 39.0 37.0 39.0 32.0 39.0 10-11 36.651268495130495 39.0 37.0 39.0 32.0 39.0 12-13 36.543096564318084 39.0 37.0 39.0 32.0 39.0 14-15 37.71236466707113 40.0 37.0 41.0 32.0 41.0 16-17 37.64726031600547 40.0 37.0 41.0 32.0 41.0 18-19 37.57458155059337 40.0 37.0 41.0 32.0 41.0 20-21 37.45876637887915 40.0 37.0 41.0 32.0 41.0 22-23 37.37049797381796 40.0 37.0 41.0 31.5 41.0 24-25 37.378631403300766 40.0 37.0 41.0 31.0 41.0 26-27 37.300994576769554 40.0 37.0 41.0 31.0 41.0 28-29 37.193056599598066 40.0 37.0 41.0 31.0 41.0 30-31 37.00718694633096 40.0 36.5 41.0 30.0 41.0 32-33 36.82916909070207 39.0 36.0 41.0 30.0 41.0 34-35 36.52261278066648 39.0 36.0 41.0 30.0 41.0 36-37 36.29199614193641 39.0 35.0 41.0 30.0 41.0 38-39 36.13686571230953 39.0 35.0 41.0 29.0 41.0 40-41 35.97584584437721 39.0 35.0 41.0 28.5 41.0 42-43 35.73732510360332 39.0 35.0 40.0 27.0 41.0 44-45 35.75266823277764 39.0 35.0 41.0 27.0 41.0 46-47 35.62143440206151 39.0 35.0 41.0 27.0 41.0 48-49 35.43772877276682 39.0 35.0 41.0 26.0 41.0 50-51 34.297591485975715 38.0 33.5 40.0 24.5 40.5 52-53 34.402377036968645 38.0 33.5 39.5 24.0 40.5 54-55 34.94374510118259 38.5 34.0 40.0 25.5 41.0 56-57 34.8670117058015 38.0 34.0 40.0 25.0 41.0 58-59 34.65987370279882 38.0 34.0 40.0 25.0 41.0 60-61 34.38430794190472 38.0 34.0 40.0 24.0 41.0 62-63 34.02805432709438 37.5 33.5 40.0 23.0 41.0 64-65 33.633514170623016 37.0 33.0 40.0 22.0 41.0 66-67 33.24161623634491 36.0 33.0 39.0 22.0 41.0 68-69 32.83924958472757 36.0 32.0 39.0 20.5 41.0 70-71 32.43633628598312 35.0 32.0 39.0 20.0 40.0 72-73 32.01491242033255 35.0 31.5 38.0 18.5 40.0 74-75 31.551596502289215 35.0 31.0 37.0 17.0 39.0 76-77 31.11060436890958 35.0 31.0 36.5 12.5 39.0 78-79 30.692211043178325 35.0 31.0 36.0 9.0 39.0 80-81 30.258665489911955 34.0 30.0 36.0 7.0 37.5 82-83 29.80509915083703 34.0 30.0 35.0 2.0 37.0 84-85 29.399733870652526 34.0 29.0 35.0 2.0 36.5 86-87 29.019115938474158 34.0 29.0 35.0 2.0 36.0 88-89 28.656612230431683 34.0 29.0 35.0 2.0 36.0 90-91 28.254742187430665 34.0 29.0 35.0 2.0 35.5 92-93 27.809849693754874 34.0 27.5 35.0 2.0 35.0 94-95 27.30687145824116 34.0 26.5 35.0 2.0 35.0 96-97 26.719009120415503 33.0 25.0 35.0 2.0 35.0 98-99 26.014665437796374 33.0 24.5 35.0 2.0 35.0 100-101 23.85856992579761 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3856061354330791 1101 2 0.19754118578884317 1101 3 0.3887704374572287 1101 4 0.360035094010307 1101 5 0.15492824524414317 1101 6 0.1473607847495373 1101 7 0.2754442304426874 1101 8 0.3322992922479884 1101 9 0.5020907096190896 1101 10-11 0.4566102678984194 1101 12-13 0.41779404724736935 1101 14-15 0.6306625845668705 1101 16-17 0.6777801085197837 1101 18-19 0.6508245577221672 1101 20-21 0.5956410222577873 1101 22-23 0.6318323508192876 1101 24-25 0.6481361476945438 1101 26-27 0.6134916950635514 1101 28-29 0.5225759031982946 1101 30-31 0.5448378640775147 1101 32-33 0.4674667975902054 1101 34-35 0.5054518239158554 1101 36-37 0.6505598394235435 1101 38-39 0.5901714558530102 1101 40-41 0.46915317513910537 1101 42-43 0.5133230818456767 1101 44-45 0.6861954141099389 1101 46-47 0.6695586035402812 1101 48-49 0.6965085571794987 1101 50-51 0.5606299458764212 1101 52-53 0.5030615652999799 1101 54-55 0.44472869147100624 1101 56-57 0.5468450802017131 1101 58-59 0.4407208855074316 1101 60-61 0.3323312315812359 1101 62-63 0.34914779728752876 1101 64-65 0.27839884914939006 1101 66-67 0.197565659274332 1101 68-69 0.15344587920213826 1101 70-71 -0.013435183587745314 1101 72-73 -0.00853628251466887 1101 74-75 0.023580502853906893 1101 76-77 -0.10002862172165194 1101 78-79 -0.229119349927128 1101 80-81 -0.2619957458814426 1101 82-83 -0.3674729424705987 1101 84-85 -0.4033645339671885 1101 86-87 -0.4043566395049645 1101 88-89 -0.5368784464845469 1101 90-91 -0.5211476575222775 1101 92-93 -0.5245772743619739 1101 94-95 -0.6411700818696886 1101 96-97 -0.747804576112852 1101 98-99 -0.7625234924271531 1101 100-101 -0.5277512866699716 1102 1 0.35292592916572474 1102 2 0.3090294047495732 1102 3 0.3640488983854304 1102 4 0.31563525267750236 1102 5 0.38291206119019705 1102 6 0.40665162171899283 1102 7 0.38258798292186214 1102 8 0.3201627591262124 1102 9 0.5207050690002859 1102 10-11 0.4222456110162298 1102 12-13 0.40028980289989136 1102 14-15 0.32537531760931415 1102 16-17 0.4759389740493276 1102 18-19 0.4957491114706798 1102 20-21 0.39817069302336705 1102 22-23 0.43347435795931233 1102 24-25 0.5343843428551907 1102 26-27 0.5113095451230478 1102 28-29 0.4969200126786646 1102 30-31 0.5852891532484747 1102 32-33 0.5437740169476015 1102 34-35 0.5673347671923281 1102 36-37 0.5462694784556703 1102 38-39 0.5043012456189757 1102 40-41 0.578026003381872 1102 42-43 0.4484571952927183 1102 44-45 0.5127714553634135 1102 46-47 0.4847265924335815 1102 48-49 0.537261948690805 1102 50-51 0.39899325408745057 1102 52-53 0.503812219802164 1102 54-55 0.4765635982540317 1102 56-57 0.44515210122511206 1102 58-59 0.37848701006118546 1102 60-61 0.45355249298543754 1102 62-63 0.4036518829678144 1102 64-65 0.3057187618824315 1102 66-67 0.3180293244865453 1102 68-69 0.23412878281180838 1102 70-71 0.1966629124059125 1102 72-73 0.27606399515292424 1102 74-75 0.14079791657862017 1102 76-77 0.14625983523987074 1102 78-79 -0.026188727158384495 1102 80-81 -0.09884679705161403 1102 82-83 -0.1487681824547309 1102 84-85 -0.2082646926343834 1102 86-87 -0.15836786520904766 1102 88-89 -0.3148638887693558 1102 90-91 -0.3209753052811024 1102 92-93 -0.283718765833612 1102 94-95 -0.40443862609817316 1102 96-97 -0.3962283761921874 1102 98-99 -0.42618390496186365 1102 100-101 -0.4375739364121358 1103 1 0.14991585330141532 1103 2 0.26712645224652576 1103 3 0.2489679296202354 1103 4 0.3311228683049805 1103 5 0.27535395057329737 1103 6 0.2983899383545534 1103 7 0.25801977903203266 1103 8 0.26618863394077863 1103 9 0.44271045883539273 1103 10-11 0.3294983556727189 1103 12-13 0.3365054892536392 1103 14-15 0.497723556353705 1103 16-17 0.4367738389273015 1103 18-19 0.5928886643052351 1103 20-21 0.41288929811985753 1103 22-23 0.5311756110073773 1103 24-25 0.5035399094500335 1103 26-27 0.49445783926417874 1103 28-29 0.509275746458485 1103 30-31 0.4390355129803325 1103 32-33 0.3952081769005531 1103 34-35 0.4910720433515863 1103 36-37 0.6183137579188411 1103 38-39 0.4270893241915701 1103 40-41 0.5030641469259436 1103 42-43 0.5805085050431629 1103 44-45 0.5796077965550879 1103 46-47 0.5723846223490625 1103 48-49 0.6608743879005061 1103 50-51 0.6376232060645108 1103 52-53 0.5998792768987258 1103 54-55 0.6632277510321387 1103 56-57 0.701991631925857 1103 58-59 0.647997844755082 1103 60-61 0.6257777833053808 1103 62-63 0.5600990512060235 1103 64-65 0.3639325482089504 1103 66-67 0.3012438724404731 1103 68-69 0.2175717412711009 1103 70-71 0.15726528663169148 1103 72-73 0.19629036325336102 1103 74-75 0.19822439000124348 1103 76-77 0.18999034379558566 1103 78-79 0.17755666888164967 1103 80-81 0.06231146414990363 1103 82-83 0.1231682800999323 1103 84-85 0.14634957261588255 1103 86-87 0.0740573103382971 1103 88-89 0.022856819973132048 1103 90-91 -0.11216881739688489 1103 92-93 -0.11654765053752314 1103 94-95 -0.15004547371922072 1103 96-97 -0.1502092783072122 1103 98-99 -0.28900264603216996 1103 100-101 -0.14088796378595703 1104 1 0.22551285601848292 1104 2 0.2183652566534704 1104 3 0.241618694726629 1104 4 0.2735232511449155 1104 5 0.20741382198205827 1104 6 0.19994745911888145 1104 7 0.2165014613304237 1104 8 0.2299356103685355 1104 9 0.2766303591472976 1104 10-11 0.3086228040047203 1104 12-13 0.37506718416244667 1104 14-15 0.4258843061151083 1104 16-17 0.43461247901852573 1104 18-19 0.3478510164165698 1104 20-21 0.43531960881662 1104 22-23 0.4369071630665431 1104 24-25 0.4449869275905627 1104 26-27 0.42588537886867783 1104 28-29 0.41100446087208553 1104 30-31 0.49744986099644706 1104 32-33 0.44339140764219565 1104 34-35 0.3921796690920516 1104 36-37 0.4873154720058608 1104 38-39 0.49000769694679036 1104 40-41 0.33926817610194604 1104 42-43 0.43038687926527786 1104 44-45 0.5376763692418436 1104 46-47 0.63239713618038 1104 48-49 0.6037677329930915 1104 50-51 0.6945619119846427 1104 52-53 0.677527038051764 1104 54-55 0.6345113604484851 1104 56-57 0.5470580592119205 1104 58-59 0.5263413746273997 1104 60-61 0.6178810871560501 1104 62-63 0.507189083634529 1104 64-65 0.4449356294305389 1104 66-67 0.4544564160623139 1104 68-69 0.5262944830919878 1104 70-71 0.45951704056015075 1104 72-73 0.3848010754672373 1104 74-75 0.20793796482194438 1104 76-77 0.16766357931820863 1104 78-79 0.31191806582484816 1104 80-81 0.3001745795566464 1104 82-83 0.3204444655786993 1104 84-85 0.29201214617916094 1104 86-87 0.32059889679389997 1104 88-89 0.27189434381008226 1104 90-91 0.2172864845921083 1104 92-93 0.22659582403971967 1104 94-95 0.21515574135387894 1104 96-97 0.17938179014942435 1104 98-99 0.11260635544497788 1104 100-101 0.30058961454320254 1105 1 0.036776123890177814 1105 2 0.03553048936714376 1105 3 6.453893618036943E-4 1105 4 0.15606895318691727 1105 5 0.06248376659851118 1105 6 0.07014331649251915 1105 7 0.08562453055811403 1105 8 0.002107757042139724 1105 9 0.048789132089268605 1105 10-11 0.018425312146213457 1105 12-13 0.048545951826049816 1105 14-15 0.17129559726406285 1105 16-17 0.016068488665986536 1105 18-19 0.06075191439781236 1105 20-21 -0.003924695325892458 1105 22-23 0.13058206224675928 1105 24-25 -0.014006099323538024 1105 26-27 -0.034550936717899106 1105 28-29 0.04851222486047391 1105 30-31 -0.06483266304829982 1105 32-33 -0.008816580632185378 1105 34-35 0.022558826609198945 1105 36-37 -0.05696382352374485 1105 38-39 0.06025333349825246 1105 40-41 -0.01929253474930448 1105 42-43 -0.07299515593808792 1105 44-45 0.03802527174749315 1105 46-47 -0.035354247556291796 1105 48-49 0.004802785728941217 1105 50-51 0.026191354110359555 1105 52-53 0.02312143448043713 1105 54-55 0.08128898389117012 1105 56-57 0.06681737723904035 1105 58-59 -0.015727902487945755 1105 60-61 0.06272261288174263 1105 62-63 0.055593045459900736 1105 64-65 0.07112547477463238 1105 66-67 -0.0031580421492947153 1105 68-69 -0.15311418236000662 1105 70-71 -0.21078081652348857 1105 72-73 -0.10090692506949495 1105 74-75 -0.1464206899125422 1105 76-77 -0.18113258478436833 1105 78-79 -0.30747875739153585 1105 80-81 -0.33457308331891866 1105 82-83 -0.2843527808699804 1105 84-85 -0.3587214789460269 1105 86-87 -0.24034882880853914 1105 88-89 -0.3951449720713107 1105 90-91 -0.31069921581392634 1105 92-93 -0.325696281672478 1105 94-95 -0.24882687294318373 1105 96-97 -0.296123775805043 1105 98-99 -0.20459740547063276 1105 100-101 0.08426291109840278 1106 1 0.03517296768374578 1106 2 0.1031806966791784 1106 3 0.15228935224515183 1106 4 0.11805419985669374 1106 5 0.011532907070659348 1106 6 0.027933730871055218 1106 7 0.11603414255579736 1106 8 0.13833391204164514 1106 9 0.17181313988275093 1106 10-11 0.12634121936957143 1106 12-13 0.10720123104561452 1106 14-15 0.18811447604566922 1106 16-17 0.14289455253964434 1106 18-19 0.23890999906721788 1106 20-21 0.21035332642286875 1106 22-23 0.12842826216816405 1106 24-25 0.07306579996814122 1106 26-27 -0.03276065495555258 1106 28-29 0.04961277748182624 1106 30-31 0.07922185337824317 1106 32-33 0.11755411130820193 1106 34-35 0.13391238944890915 1106 36-37 0.2605017576505162 1106 38-39 0.1502908291812446 1106 40-41 0.2460797322655921 1106 42-43 0.29533634130306297 1106 44-45 0.229722888317049 1106 46-47 0.31197912321930943 1106 48-49 0.3142296500339157 1106 50-51 0.3475884097663666 1106 52-53 0.3157726084791932 1106 54-55 0.28347404875033533 1106 56-57 0.3965447362964838 1106 58-59 0.3396497353354775 1106 60-61 0.426296688210968 1106 62-63 0.14680526767347146 1106 64-65 0.0378598732227573 1106 66-67 0.0753628698207578 1106 68-69 0.1803939877233418 1106 70-71 0.09658090353491389 1106 72-73 -0.07939906432590149 1106 74-75 -0.2104689048648538 1106 76-77 -0.2515470015458803 1106 78-79 0.1044242878260242 1106 80-81 -0.037985619782855196 1106 82-83 0.037575548167538386 1106 84-85 0.0702296196438148 1106 86-87 0.11658447627646851 1106 88-89 0.14176117904086638 1106 90-91 0.22927515485831407 1106 92-93 0.4063669747782619 1106 94-95 0.32581501557012515 1106 96-97 0.4102073459352944 1106 98-99 0.4286617196477387 1106 100-101 0.5516551711061091 1107 1 0.0145416851119613 1107 2 0.059623769846268715 1107 3 -0.010081470362948153 1107 4 0.018885112899617695 1107 5 -0.03501115785370246 1107 6 -0.02029867879409153 1107 7 -0.15210930185803306 1107 8 -0.08049760990539312 1107 9 -0.03455923371298297 1107 10-11 -0.04260888586055245 1107 12-13 0.016057213095500344 1107 14-15 0.07318075608179697 1107 16-17 0.0743080646965808 1107 18-19 0.17958677910486642 1107 20-21 0.1147158616986701 1107 22-23 0.16293416419291873 1107 24-25 0.017090397788990686 1107 26-27 0.08640125305372948 1107 28-29 -0.1092712822947135 1107 30-31 0.01380042546777105 1107 32-33 0.10511073412212824 1107 34-35 0.0538267011353426 1107 36-37 0.17373248770461203 1107 38-39 0.2275527932160628 1107 40-41 0.1374677954640049 1107 42-43 0.17267770972629393 1107 44-45 0.15798891618776878 1107 46-47 0.21622256273938234 1107 48-49 0.16742850428943257 1107 50-51 0.27439923186055637 1107 52-53 0.22132631785003554 1107 54-55 0.16858233710757986 1107 56-57 0.057394381297292796 1107 58-59 0.05944351489919342 1107 60-61 0.10780973554799544 1107 62-63 0.07641776328676997 1107 64-65 0.1023937490360538 1107 66-67 0.20987102383798373 1107 68-69 0.18359744701474767 1107 70-71 0.24123089121391672 1107 72-73 0.16036855314834497 1107 74-75 0.07374510428949321 1107 76-77 -0.00841456807601304 1107 78-79 0.08108718577366503 1107 80-81 0.0690732000951364 1107 82-83 0.17096034049124498 1107 84-85 0.08924101948955254 1107 86-87 0.002316537676751551 1107 88-89 -0.004996731335321414 1107 90-91 0.09358279204327147 1107 92-93 0.052086911508823164 1107 94-95 0.0013875305216970446 1107 96-97 0.029779556867879364 1107 98-99 0.10851341728194797 1107 100-101 0.25515755352647673 1108 1 0.021238557816420922 1108 2 -0.03553964707971957 1108 3 -0.04697542608618477 1108 4 0.00990161195044692 1108 5 0.011413242455518002 1108 6 -0.02002947366280239 1108 7 -0.014860547397056223 1108 8 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-2.506824148985732 2316 54-55 -2.226119149060551 2316 56-57 -2.233995451359746 2316 58-59 -2.024909014824793 2316 60-61 -1.8639883697476662 2316 62-63 -2.1149093455696146 2316 64-65 -2.2661077953688817 2316 66-67 -2.2609363340811512 2316 68-69 -2.4195953168111544 2316 70-71 -2.205141828106015 2316 72-73 -2.1267545549399145 2316 74-75 -2.246276307777322 2316 76-77 -2.2502140037814513 2316 78-79 -2.0935010775018803 2316 80-81 -2.0143096993698144 2316 82-83 -1.9213742713742512 2316 84-85 -2.0004485871233335 2316 86-87 -2.0763709252192477 2316 88-89 -2.176862831866604 2316 90-91 -2.3550529731368925 2316 92-93 -2.6476560119035035 2316 94-95 -2.630206626720188 2316 96-97 -2.586104908338765 2316 98-99 -2.8652141901015753 2316 100-101 -3.1348439776998696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15963.0 3 4894.0 4 5059.0 5 5732.0 6 6478.0 7 8089.0 8 9778.0 9 12142.0 10 14643.0 11 18842.0 12 23721.0 13 29396.0 14 30974.0 15 28590.0 16 33645.0 17 41360.0 18 37253.0 19 30052.0 20 31681.0 21 35045.0 22 39430.0 23 44915.0 24 52354.0 25 60810.0 26 70638.0 27 82076.0 28 97527.0 29 114078.0 30 135573.0 31 164524.0 32 201976.0 33 252901.0 34 331740.0 35 458936.0 36 678466.0 37 1008888.0 38 924487.0 39 153234.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1883045970381 19.068767761988504 13.90092512701252 23.84200251396088 2 15.356091162361693 20.74934130695285 39.857261122178585 24.037306408506875 3 18.780979603238066 24.662745552365134 30.414446069999844 26.141828774396952 4 12.903195928591574 16.694320760266418 37.54427868370295 32.85820462743906 5 13.811304859278659 37.18539923085984 34.524376933508755 14.47891897635275 6 31.548194516698537 36.007244676715366 18.037019784208674 14.407541022377421 7 27.998014226090334 32.788467096640616 21.575078386309343 17.638440290959704 8 25.766334788664626 34.80366440467215 20.73857480554599 18.691426001117236 9 26.561942406316703 16.601393472957703 20.88486534062368 35.95179878010191 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28.18029293444586 22.275826795432543 36-37 22.705089033733334 26.559120821825623 28.211611729572873 22.524178414868178 38-39 22.446342137505333 26.824682208915736 28.391353889700653 22.33762176387828 40-41 22.646356033452808 26.54241338112306 28.40990119663955 22.401329388784585 42-43 22.45597141741703 26.689863267533752 28.063410112792237 22.79075520225698 44-45 22.54505096305712 26.75523978011658 28.000208690043117 22.699500566783186 46-47 22.539283501711864 26.85601745623538 27.781589507362963 22.823109534689788 48-49 22.663325831951333 26.904465536019917 27.65949291669671 22.77271571533204 50-51 22.406964060023316 27.057914400095544 27.722909125198015 22.812212414683124 52-53 22.681696937314506 27.03462128264162 27.508897904538266 22.774783875505612 54-55 22.390687933238706 26.764935028838387 27.71865425207014 23.125722785852762 56-57 22.4018287601999 27.122885545810338 27.617196324649512 22.858089369340252 58-59 22.588635794496586 26.735306748323733 27.649018098334576 23.0270393588451 60-61 22.59010682367581 26.68425922889106 27.941347930628975 22.784286016804153 62-63 22.40098109560418 26.69129206516167 27.922650513619296 22.985076325614855 64-65 22.24580926923189 26.678911098856346 27.815960054698586 23.259319577213173 66-67 22.22310043782896 26.860806294914518 27.561827933778073 23.354265333478445 68-69 22.214519888722002 26.61716739109723 27.826427424280208 23.34188529590056 70-71 22.17057453119243 26.514604519390687 27.795335462270454 23.51948548714643 72-73 22.257013732140756 26.38572677910246 27.748854785175737 23.608404703581048 74-75 22.064602594745082 26.67734148305628 27.596253611929782 23.661802310268847 76-77 22.147787245542556 26.42805926378714 27.756325668721953 23.667827821948347 78-79 22.04419479256966 26.451241401344376 27.7912836230349 23.713280183051065 80-81 22.03134071897846 26.47133913327829 27.73321315395195 23.7641069937913 82-83 22.119825896315373 26.349093462120006 27.74919038030571 23.781890261258916 84-85 21.99347329535226 26.196507190548246 27.705544436167344 24.104475077932147 86-87 21.97975197180461 26.05614603714371 27.96002149904011 24.004080492011568 88-89 22.04639071536784 25.950933579539186 27.852919325745845 24.14975637934713 90-91 21.954033097467697 25.967524904122957 28.0075572636626 24.070884734746745 92-93 22.094903229142403 25.814951558831716 27.94831740291012 24.141827809115753 94-95 21.96565285115037 25.557470129624182 28.0228524303004 24.45402458892505 96-97 22.048222344366238 25.366766510387478 28.068391667855057 24.516619477391227 98-99 21.902266318650817 25.348427766518693 27.938850486991008 24.81045542783948 100-101 22.070236925616005 24.928884560244434 28.336382978662854 24.664495535476707 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2804.0 1 1600.0 2 523.5 3 666.5 4 656.5 5 699.5 6 1053.5 7 1914.5 8 3043.0 9 3806.0 10 3835.5 11 3869.0 12 4305.5 13 4849.0 14 5392.5 15 5839.0 16 6255.0 17 6592.5 18 6895.0 19 7266.5 20 7726.0 21 8609.0 22 9985.5 23 11870.5 24 14129.5 25 17770.5 26 24071.0 27 31733.0 28 38884.0 29 46960.5 30 56880.0 31 67316.5 32 77617.0 33 87707.0 34 98814.0 35 110012.0 36 122473.5 37 135218.0 38 143044.5 39 147309.5 40 152044.0 41 160012.5 42 168531.0 43 177569.0 44 186499.5 45 192076.5 46 196187.0 47 202897.5 48 217326.5 49 223106.0 50 215655.0 51 207361.5 52 197468.5 53 192370.0 54 187068.0 55 171856.0 56 154349.0 57 136480.5 58 121320.0 59 107612.0 60 90853.0 61 73578.0 62 58189.0 63 45414.5 64 34213.5 65 25579.0 66 18744.5 67 14440.0 68 10737.5 69 7279.5 70 5321.5 71 3938.0 72 3061.5 73 2385.5 74 1777.5 75 1360.5 76 929.0 77 527.0 78 306.5 79 198.5 80 144.0 81 114.5 82 87.0 83 72.5 84 62.5 85 47.0 86 37.5 87 25.5 88 18.5 89 15.5 90 17.0 91 15.5 92 10.0 93 6.0 94 4.5 95 3.5 96 6.0 97 7.0 98 3.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16327660476808473 2 0.0970747108954231 3 0.089465080766877 4 0.10508087261875695 5 0.12534174886672214 6 0.039709806849460176 7 0.04280652978018366 8 0.0473949668055849 9 0.0477348510296887 10-11 0.046129842193642995 12-13 0.050652190619912954 14-15 0.04789535191329326 16-17 0.04681905187029791 18-19 0.05125642924054192 20-21 0.05346567669721659 22-23 0.06700439829068447 24-25 0.08409302178034755 26-27 0.08638724029304817 28-29 0.07570921091912058 30-31 0.0635677911358572 32-33 0.05226664068440598 34-35 0.3021665164567221 36-37 0.4789063129906961 38-39 0.45582250943697994 40-41 0.4309165487929295 42-43 0.46184601318637497 44-45 0.4710417696940721 46-47 0.47037144247431184 48-49 0.48680862120110935 50-51 0.4758851198875512 52-53 0.482994364908389 54-55 0.47361922506019255 56-57 0.4519327233166809 58-59 0.4307371654524303 60-61 0.399439493149539 62-63 0.4581544928634699 64-65 0.4968918531828553 66-67 0.4984496558766643 68-69 0.5089766255954347 70-71 0.505700519324212 72-73 0.49616487859241104 74-75 0.4887723967181535 76-77 0.46974832139678824 78-79 0.4439737677355836 80-81 0.4494402390065864 82-83 0.46194986669929555 84-85 0.45975006047106814 86-87 0.4718348328836476 88-89 0.4821540955765768 90-91 0.4864026483778742 92-93 0.4884608361793916 94-95 0.4930115082910036 96-97 0.4944465750149974 98-99 0.4881964817828665 100-101 0.47375140225845513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5295921.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.36184235716778 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39365568950893 35.01090564115113 2 14.457797418448278 13.98411439165726 3 5.332393617709936 7.7365313857816655 4 2.5242528649366553 4.883100764947827 5 1.4235318588170174 3.4422311673257306 6 0.8401272613208546 2.4378061303174965 7 0.5681638437317077 1.9234215170516749 8 0.39959549191827 1.5460139349429027 9 0.2969417153239789 1.2924583583188731 >10 1.5568560399362756 13.767786895181858 >50 0.1237418123069921 4.131834351724075 >100 0.07687159824628009 6.856616893409745 >500 0.004621635305041051 1.4905320201721048 >1k 0.0013316536391145362 0.9863615605044898 >5k 7.833256700575669E-5 0.28270242905098975 >10k+ 3.916628350287834E-5 0.22758255846215356 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12015 0.22687271958928393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9146 0.17269895075851774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5779 0.10912171839421321 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.776491378931068E-5 0.0 4 0.0 0.0 0.0 3.776491378931068E-5 0.0 5 0.0 0.0 0.0 3.776491378931068E-5 0.0 6 0.0 0.0 0.0 3.776491378931068E-5 0.0 7 1.888245689465534E-5 0.0 0.0 3.776491378931068E-5 0.0 8 1.888245689465534E-5 0.0 0.0 3.776491378931068E-5 0.0 9 1.888245689465534E-5 0.0 0.0 3.776491378931068E-5 0.0 10-11 1.888245689465534E-5 1.888245689465534E-5 0.0 6.608859913129368E-5 0.0 12-13 1.888245689465534E-5 1.888245689465534E-5 0.0 7.552982757862136E-5 0.0 14-15 1.888245689465534E-5 1.888245689465534E-5 0.0 9.44122844732767E-5 9.44122844732767E-6 16-17 1.888245689465534E-5 1.888245689465534E-5 1.888245689465534E-5 1.1329474136793204E-4 3.776491378931068E-5 18-19 1.888245689465534E-5 1.888245689465534E-5 1.888245689465534E-5 1.4161842670991505E-4 3.776491378931068E-5 20-21 1.888245689465534E-5 7.552982757862136E-5 1.888245689465534E-5 2.1714825428853642E-4 3.776491378931068E-5 22-23 1.888245689465534E-5 7.552982757862136E-5 1.888245689465534E-5 4.720614223663835E-4 3.776491378931068E-5 24-25 1.888245689465534E-5 9.44122844732767E-5 1.888245689465534E-5 8.497105602594903E-4 3.776491378931068E-5 26-27 1.888245689465534E-5 1.227359698152597E-4 1.888245689465534E-5 0.0017466272627556189 3.776491378931068E-5 28-29 3.776491378931068E-5 1.3217719826258737E-4 1.888245689465534E-5 0.006571094999340058 3.776491378931068E-5 30-31 5.664737068396602E-5 1.7938334049922574E-4 1.888245689465534E-5 0.023999602713106936 4.720614223663835E-5 32-33 6.608859913129368E-5 2.0770702584120874E-4 1.888245689465534E-5 0.06279361040317633 7.552982757862136E-5 34-35 7.552982757862136E-5 2.0770702584120874E-4 1.888245689465534E-5 0.12232055576357728 7.552982757862136E-5 36-37 7.552982757862136E-5 2.0770702584120874E-4 1.888245689465534E-5 0.21962185614173627 7.552982757862136E-5 38-39 8.497105602594903E-5 2.2658948273586408E-4 1.888245689465534E-5 0.3602583950931292 8.497105602594903E-5 40-41 9.44122844732767E-5 2.2658948273586408E-4 1.888245689465534E-5 0.5137161222759932 9.44122844732767E-5 42-43 9.44122844732767E-5 2.3603071118319174E-4 1.888245689465534E-5 0.6764545770225803 9.44122844732767E-5 44-45 9.44122844732767E-5 2.454719396305194E-4 1.888245689465534E-5 0.8785629543945237 9.44122844732767E-5 46-47 9.44122844732767E-5 2.454719396305194E-4 1.888245689465534E-5 1.1178320069351488 9.44122844732767E-5 48-49 9.44122844732767E-5 2.832368534198301E-4 1.888245689465534E-5 1.368222826586726 9.44122844732767E-5 50-51 9.44122844732767E-5 2.832368534198301E-4 1.888245689465534E-5 1.6274978422072384 9.44122844732767E-5 52-53 1.0385351292060437E-4 3.210017672091408E-4 1.888245689465534E-5 1.8915784431074405 1.1329474136793203E-4 54-55 1.1329474136793204E-4 3.587666809984514E-4 1.888245689465534E-5 2.195453821913129 1.3217719826258737E-4 56-57 1.1329474136793204E-4 3.587666809984514E-4 1.888245689465534E-5 2.5462804297873776 1.3217719826258737E-4 58-59 1.3217719826258737E-4 3.776491378931068E-4 1.888245689465534E-5 2.91829315429743 1.3217719826258737E-4 60-61 1.3217719826258737E-4 3.776491378931068E-4 1.888245689465534E-5 3.310765020852841 1.3217719826258737E-4 62-63 1.3217719826258737E-4 3.776491378931068E-4 1.888245689465534E-5 3.7101950727739332 1.3217719826258737E-4 64-65 1.3217719826258737E-4 3.776491378931068E-4 1.888245689465534E-5 4.137684455640483 1.3217719826258737E-4 66-67 1.888245689465534E-4 3.870903663404344E-4 1.888245689465534E-5 4.617723716044859 1.3217719826258737E-4 68-69 1.888245689465534E-4 3.965315947877621E-4 1.888245689465534E-5 5.143713435302377 1.3217719826258737E-4 70-71 1.9826579739388105E-4 4.059728232350898E-4 1.888245689465534E-5 5.709441285094698 1.510596551572427E-4 72-73 2.0770702584120874E-4 4.342965085770728E-4 1.888245689465534E-5 6.274649867322417 1.888245689465534E-4 74-75 2.0770702584120874E-4 4.342965085770728E-4 1.888245689465534E-5 6.872827219288203 1.888245689465534E-4 76-77 2.0770702584120874E-4 4.342965085770728E-4 1.888245689465534E-5 7.515453874784008 1.888245689465534E-4 78-79 2.0770702584120874E-4 4.342965085770728E-4 1.888245689465534E-5 8.21673888262306 1.888245689465534E-4 80-81 2.454719396305194E-4 4.342965085770728E-4 1.888245689465534E-5 8.954731386665323 2.0770702584120874E-4 82-83 2.6435439652517474E-4 4.342965085770728E-4 1.888245689465534E-5 9.70669690880963 2.832368534198301E-4 84-85 2.737956249725024E-4 4.342965085770728E-4 1.888245689465534E-5 10.487042763666603 2.832368534198301E-4 86-87 2.832368534198301E-4 4.5317896547172816E-4 1.888245689465534E-5 11.298667408369575 3.021193103144854E-4 88-89 2.832368534198301E-4 4.5317896547172816E-4 1.888245689465534E-5 12.156110334727426 3.021193103144854E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 22165 0.0 28.125452 1 GGTATCA 15535 0.0 27.403536 1 GTACATG 18410 0.0 26.677647 1 TACATGG 19095 0.0 24.863583 2 ACATGGG 19160 0.0 23.790041 3 ATCAACG 26235 0.0 23.623394 3 TCAACGC 26345 0.0 23.56073 4 CAACGCA 26510 0.0 23.396433 5 TATCAAC 26760 0.0 23.159933 2 AACGCAG 26985 0.0 22.94925 6 ACGCAGA 29755 0.0 20.574919 7 GAGTACT 13000 0.0 20.213955 12-13 CGCAGAG 30100 0.0 20.165873 8 CATGGGA 9615 0.0 19.761082 4 AGAGTAC 23030 0.0 19.294785 10-11 CATGGGG 9740 0.0 19.06965 4 AGTACTT 14015 0.0 18.597984 12-13 GCAGAGT 31555 0.0 18.53098 9 GTACTTT 14660 0.0 18.038187 14-15 GAGTACA 15530 0.0 17.002989 1 >>END_MODULE