FastQCFastQC Report
Thu 26 May 2016
SRR938765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5295921
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113350.21403264890091828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95130.17962881243885626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61880.11684464326412725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158100.029.6486341
GTATCAA225500.028.3285061
GTACATG188300.024.134571
ATCAACG267900.023.609893
TCAACGC268750.023.5175614
CAACGCA270650.023.3699975
TATCAAC275600.023.0707722
TACATGG192700.022.8754962
AACGCAG277800.022.802666
ACATGGG193800.021.790783
GAGTACT135000.020.75656512-13
ACGCAGA307650.020.4514057
CGCAGAG309750.020.0829688
AGAGTAC240800.019.28286710-11
GTACTTT149700.018.68664414-15
GCAGAGT326650.018.6811249
CATGGGG97400.018.2200324
AGTACTT144750.018.21104212-13
CATGGGA103450.017.4755554
GAGTACA160800.016.5898881
Produced by FastQC (version 0.11.5)