##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938765_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5295921 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02936070987464 33.0 31.0 34.0 30.0 34.0 2 32.25371866385469 34.0 31.0 34.0 30.0 34.0 3 32.35103828021604 34.0 31.0 34.0 30.0 34.0 4 35.97707820792644 37.0 35.0 37.0 35.0 37.0 5 35.87873837241907 37.0 35.0 37.0 35.0 37.0 6 35.89713366192585 37.0 35.0 37.0 35.0 37.0 7 35.82794229747763 37.0 35.0 37.0 33.0 37.0 8 35.79471748162406 37.0 35.0 37.0 33.0 37.0 9 37.45668166122569 39.0 37.0 39.0 34.0 39.0 10-11 37.38577397585802 39.0 37.0 39.0 34.0 39.0 12-13 37.32004659812712 39.0 37.0 39.0 34.0 39.0 14-15 38.57226561725524 40.0 38.0 41.0 34.0 41.0 16-17 38.51021635330285 40.0 38.0 41.0 34.0 41.0 18-19 38.51489778642846 40.0 38.0 41.0 33.5 41.0 20-21 38.57129808016396 40.0 38.0 41.0 34.0 41.0 22-23 38.52729043730071 40.0 38.0 41.0 34.0 41.0 24-25 38.461432204143534 40.0 38.0 41.0 34.0 41.0 26-27 38.31938873332892 40.0 38.0 41.0 33.5 41.0 28-29 38.17287880616044 40.0 38.0 41.0 33.0 41.0 30-31 37.97600162464659 40.0 37.0 41.0 33.0 41.0 32-33 37.794762421871475 40.0 37.0 41.0 33.0 41.0 34-35 37.78998223349631 40.0 37.0 41.0 33.0 41.0 36-37 37.732622994187416 40.0 37.0 41.0 33.0 41.0 38-39 37.65591084157033 40.0 37.0 41.0 32.5 41.0 40-41 37.52217612385079 40.0 37.0 41.0 32.0 41.0 42-43 37.37415069069195 40.0 37.0 41.0 31.5 41.0 44-45 37.16349431949608 40.0 36.0 41.0 31.0 41.0 46-47 36.92562407936221 40.0 36.0 41.0 31.0 41.0 48-49 36.69948220526703 39.5 36.0 41.0 30.0 41.0 50-51 36.49067036687292 39.0 35.5 41.0 30.0 41.0 52-53 36.29940401301303 39.0 35.0 41.0 30.0 41.0 54-55 36.06975406166369 39.0 35.0 40.5 29.0 41.0 56-57 35.825036287361534 38.5 35.0 40.0 29.0 41.0 58-59 35.53382659975479 38.0 34.5 40.0 28.0 41.0 60-61 35.20781890439831 38.0 34.0 40.0 28.0 41.0 62-63 34.85137665384359 37.5 34.0 40.0 27.5 41.0 64-65 34.49943598101255 37.0 34.0 40.0 26.5 41.0 66-67 34.11554364198408 36.0 33.5 39.0 26.0 41.0 68-69 33.68000098566425 36.0 33.0 39.0 26.0 40.0 70-71 33.22560306318769 35.0 32.5 38.5 25.5 40.0 72-73 32.89393204694707 35.0 32.0 37.5 25.0 40.0 74-75 32.48300947087391 35.0 32.0 37.0 24.5 39.0 76-77 30.39495793083016 33.5 29.5 35.0 20.5 37.5 78-79 31.471046867957433 34.5 31.0 36.0 22.5 38.0 80-81 31.535311704989557 35.0 31.0 36.0 23.0 37.5 82-83 31.245821548319924 34.5 31.0 35.0 22.5 37.0 84-85 30.88196170222328 34.0 31.0 35.0 20.5 36.5 86-87 30.37421111455401 34.0 31.0 35.0 18.5 36.0 88-89 29.89422670768692 34.0 30.0 35.0 14.5 36.0 90-91 29.5166360109979 34.0 30.0 35.0 8.5 35.0 92-93 29.26553964834445 34.0 29.5 35.0 4.5 35.0 94-95 28.91903381866912 34.0 29.0 35.0 2.0 35.0 96-97 28.456666460847885 34.0 29.0 35.0 2.0 35.0 98-99 27.788053107287666 34.0 29.0 35.0 2.0 35.0 100-101 24.810858961075894 31.0 22.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02412612391297131 1101 2 0.07316428175154499 1101 3 0.09901561098918421 1101 4 0.1449123979588407 1101 5 0.043215458429706644 1101 6 0.12832892474934 1101 7 0.12039482598295592 1101 8 0.12554311553231656 1101 9 0.1445519450194226 1101 10-11 0.17411126560001122 1101 12-13 0.15049297014731167 1101 14-15 0.22928333553161195 1101 16-17 0.1681518195589362 1101 18-19 0.15315380593519734 1101 20-21 0.1706330488053922 1101 22-23 0.23477472833906177 1101 24-25 0.19767478576807918 1101 26-27 0.17567960466713828 1101 28-29 0.2090071837362686 1101 30-31 0.1464647256755569 1101 32-33 0.16790009506161852 1101 34-35 0.1854244636924065 1101 36-37 0.22610063057528862 1101 38-39 0.26808084031925006 1101 40-41 0.29681778476156495 1101 42-43 0.21047103969720382 1101 44-45 0.27719638122997026 1101 46-47 0.3275392341171681 1101 48-49 0.2864648862079733 1101 50-51 0.3239682596575122 1101 52-53 0.35111088462353734 1101 54-55 0.31980585849353815 1101 56-57 0.15608945310206224 1101 58-59 0.28031779650778077 1101 60-61 0.2965652137006245 1101 62-63 0.2619825912947107 1101 64-65 0.21164523457168372 1101 66-67 0.21390359460518482 1101 68-69 0.13318526946856935 1101 70-71 0.10453418254540736 1101 72-73 0.10805943810927232 1101 74-75 0.08656192862672896 1101 76-77 0.07110535841216503 1101 78-79 0.08572183165378533 1101 80-81 0.21201023399784447 1101 82-83 0.21747901939352587 1101 84-85 0.2026641118538528 1101 86-87 0.25284229320724094 1101 88-89 0.31639573122399867 1101 90-91 0.3566422290945255 1101 92-93 0.3024286987542517 1101 94-95 0.27918832066861654 1101 96-97 0.23661831408859157 1101 98-99 0.2469495470103027 1101 100-101 0.9962828230138889 1102 1 0.07453318460317604 1102 2 0.07948042613868722 1102 3 0.11085051777220656 1102 4 0.13569946658240184 1102 5 0.0904003850461308 1102 6 0.08956724287706663 1102 7 0.07809288230981082 1102 8 0.09359444437008335 1102 9 0.1550956974985951 1102 10-11 0.16721483561190809 1102 12-13 0.1648596500362416 1102 14-15 0.204251840093292 1102 16-17 0.239453564898092 1102 18-19 0.16071528947347957 1102 20-21 0.2507166664174534 1102 22-23 0.19316052762506075 1102 24-25 0.11301008921908817 1102 26-27 0.13146199260843616 1102 28-29 0.08712812780607493 1102 30-31 0.22991046149468275 1102 32-33 0.21988688601759776 1102 34-35 0.22870292462061315 1102 36-37 0.25465231641424424 1102 38-39 0.1915562469039429 1102 40-41 0.24692290177861764 1102 42-43 0.2774990841240239 1102 44-45 0.16848920153936575 1102 46-47 0.11061773471217151 1102 48-49 0.1234926926339952 1102 50-51 0.11361950916167274 1102 52-53 0.15577915118442576 1102 54-55 0.16251922620476478 1102 56-57 0.12034768396284079 1102 58-59 0.022339732247566246 1102 60-61 -0.05184335432793574 1102 62-63 -0.1055898402800679 1102 64-65 -0.11532922000983348 1102 66-67 -0.1413697493059587 1102 68-69 -0.04712004588248675 1102 70-71 -0.2066206766374563 1102 72-73 -0.1288454409069928 1102 74-75 -0.18895703210313997 1102 76-77 -0.30280511362274254 1102 78-79 -0.26634242856041723 1102 80-81 -0.16094760019493748 1102 82-83 -0.13210134554102027 1102 84-85 -0.010076229280617355 1102 86-87 -0.15388940056507394 1102 88-89 -0.04007884235791437 1102 90-91 -0.028275184630189187 1102 92-93 -0.03864048014301247 1102 94-95 -0.10055305180262408 1102 96-97 -0.016155942158935233 1102 98-99 -0.03111867988378947 1102 100-101 0.44599793606426275 1103 1 0.10287784805090183 1103 2 0.12840566106188334 1103 3 0.08043003105188262 1103 4 0.09857634780210134 1103 5 0.08831671234820249 1103 6 0.1370364482603108 1103 7 0.16021520215850416 1103 8 0.1477252471937618 1103 9 0.1337798447059413 1103 10-11 0.10071753519249427 1103 12-13 0.11857322093100464 1103 14-15 0.1726717367855315 1103 16-17 0.1561927286498488 1103 18-19 0.1255284140856716 1103 20-21 0.1756211365796858 1103 22-23 0.07876657786884067 1103 24-25 0.14227259141071613 1103 26-27 0.22170186172043316 1103 28-29 0.21613288906541328 1103 30-31 0.15689199840282697 1103 32-33 0.19406169380770422 1103 34-35 0.14663527873942428 1103 36-37 0.04768589703297721 1103 38-39 0.20574855191799912 1103 40-41 0.13472138977724057 1103 42-43 0.27786618076303427 1103 44-45 0.2618556288788696 1103 46-47 0.24980051938362635 1103 48-49 0.31653745674088896 1103 50-51 0.2643364414756988 1103 52-53 0.2855287529620938 1103 54-55 0.19687670488851694 1103 56-57 0.21260982927761773 1103 58-59 7.870755046397448E-4 1103 60-61 0.0892940538260234 1103 62-63 0.23213102389659213 1103 64-65 0.17274978002622987 1103 66-67 0.1186609613763423 1103 68-69 0.12467962683533784 1103 70-71 0.07147117314101337 1103 72-73 0.11415019046037145 1103 74-75 0.19312948348567005 1103 76-77 0.11496131452501501 1103 78-79 0.047478101246259286 1103 80-81 0.1042788860354591 1103 82-83 0.07396992848443418 1103 84-85 0.06503056953410535 1103 86-87 0.07984965997840021 1103 88-89 0.34855137385722657 1103 90-91 0.46357342031709337 1103 92-93 0.32386490565080095 1103 94-95 0.20088267803538784 1103 96-97 0.2772739567218174 1103 98-99 0.3734699231858478 1103 100-101 0.8585953621988374 1104 1 0.0372042290545096 1104 2 0.06121723765371456 1104 3 -0.010520033147152219 1104 4 0.005869185942664501 1104 5 0.09469930206537214 1104 6 0.09855796384042748 1104 7 -0.016737005328430143 1104 8 0.020284921810002743 1104 9 -0.04141639697942168 1104 10-11 0.09245621071844567 1104 12-13 0.09686447024359524 1104 14-15 0.10440461417594094 1104 16-17 0.16554371249173272 1104 18-19 0.17015987179308212 1104 20-21 0.060147086933646676 1104 22-23 0.046577116168833754 1104 24-25 0.05028212256762288 1104 26-27 0.12175204834132103 1104 28-29 0.13155979301799192 1104 30-31 0.06491284814811848 1104 32-33 0.10355137563161776 1104 34-35 0.11050919313588992 1104 36-37 0.08647043608270621 1104 38-39 0.18690223450373367 1104 40-41 0.24046211366585624 1104 42-43 0.21440211999760805 1104 44-45 0.18711812203489586 1104 46-47 0.19562120985374065 1104 48-49 0.18085238861506525 1104 50-51 0.2911946028117569 1104 52-53 0.1465407132389842 1104 54-55 0.13559696190197457 1104 56-57 0.08860148853437977 1104 58-59 0.08681325192853961 1104 60-61 0.09101851622325796 1104 62-63 -0.033829713731286404 1104 64-65 0.12586903584262643 1104 66-67 -0.013316759717945104 1104 68-69 0.007672059378059259 1104 70-71 0.011195803958848671 1104 72-73 0.017172166782479792 1104 74-75 -0.01460288939752985 1104 76-77 -0.05966373459765961 1104 78-79 -0.03256855925705793 1104 80-81 -0.050483719400961746 1104 82-83 -0.006108367473405707 1104 84-85 0.05895003521222009 1104 86-87 0.2511708508810315 1104 88-89 0.37248292552401807 1104 90-91 0.36246075403193956 1104 92-93 0.30380204749293327 1104 94-95 0.37593894651109494 1104 96-97 0.3318236481921879 1104 98-99 0.33522607310311514 1104 100-101 0.8439622761238432 1105 1 -0.08926833549449142 1105 2 -0.08261217073017946 1105 3 -0.10493198423974803 1105 4 -0.07780795602346302 1105 5 -0.06015091325170374 1105 6 -0.15827584631644953 1105 7 -0.09197947182389754 1105 8 -0.08381794641458384 1105 9 -0.05317979795864858 1105 10-11 -0.00786722612757984 1105 12-13 -0.046205914038914386 1105 14-15 -0.06623940398358741 1105 16-17 -0.10167415196856666 1105 18-19 -0.1542961748266336 1105 20-21 -0.06494401929772664 1105 22-23 -0.10222357870210175 1105 24-25 -0.09162659938004936 1105 26-27 -0.1471558705156184 1105 28-29 -0.1143306000267259 1105 30-31 -0.1820626694764229 1105 32-33 -0.18100129008650612 1105 34-35 1.5897696674471717E-4 1105 36-37 -0.03832522552320938 1105 38-39 -0.05710249942680434 1105 40-41 0.009047488493763467 1105 42-43 -0.025882827174669387 1105 44-45 -0.034279263248677694 1105 46-47 0.00570664465968207 1105 48-49 -0.0659726282206492 1105 50-51 -0.04691854295888476 1105 52-53 -0.08881012345648998 1105 54-55 -0.11328086717018238 1105 56-57 -0.08082666848316222 1105 58-59 -0.13802822119134106 1105 60-61 -0.11004194289806435 1105 62-63 -0.14564414975954065 1105 64-65 -0.07955114865263369 1105 66-67 -0.11645727496003389 1105 68-69 -0.18818229497929906 1105 70-71 -0.22068190056348413 1105 72-73 -0.391573805445951 1105 74-75 -0.24612091092694044 1105 76-77 -0.3486705938771806 1105 78-79 -0.3729453480307079 1105 80-81 -0.3118440561137348 1105 82-83 -0.30407538614706553 1105 84-85 -0.26681630369058595 1105 86-87 -0.24387236627717712 1105 88-89 -0.043437127567845124 1105 90-91 0.1120600051622418 1105 92-93 0.028129891520695338 1105 94-95 -0.04519875128213258 1105 96-97 -0.1358765300822462 1105 98-99 -0.0160131309427598 1105 100-101 0.28616901000888717 1106 1 -0.04825927922038531 1106 2 -0.08997251610860246 1106 3 -0.13688175240401534 1106 4 0.003266773197658779 1106 5 -0.05667128211289452 1106 6 -0.11412480089070698 1106 7 -0.09959997722683056 1106 8 -0.113234186722174 1106 9 -0.11852730603140316 1106 10-11 -0.11576810008588723 1106 12-13 -0.07200729733454381 1106 14-15 -0.01524811269154469 1106 16-17 -0.16846228055569412 1106 18-19 -0.22645547950614997 1106 20-21 -0.24094830388853694 1106 22-23 -0.17750031942555466 1106 24-25 -0.22535039573746474 1106 26-27 -0.2399643387795507 1106 28-29 -0.17794226219418618 1106 30-31 -0.15199308861561178 1106 32-33 -0.14640257975687376 1106 34-35 -0.14478761120250283 1106 36-37 -0.15920024829364365 1106 38-39 -0.14621969464444362 1106 40-41 -0.08155248654145453 1106 42-43 -0.0635133317204506 1106 44-45 -0.04510457406235702 1106 46-47 -0.060882890452944594 1106 48-49 -0.04648249629106971 1106 50-51 -0.03950839299820075 1106 52-53 -0.04371529038601807 1106 54-55 -0.029395211433858037 1106 56-57 -0.10244512847989284 1106 58-59 -0.10662446562443506 1106 60-61 -0.1082976063223029 1106 62-63 -0.17225802773471344 1106 64-65 -0.16537050864574354 1106 66-67 -0.19484523993818215 1106 68-69 -0.17949218563269653 1106 70-71 -0.18600758283479735 1106 72-73 -0.08432283166528265 1106 74-75 0.020087301190734763 1106 76-77 -0.039968046249665434 1106 78-79 -0.06336414465496532 1106 80-81 -0.1465705455206887 1106 82-83 0.021417995320160088 1106 84-85 -0.06106030541782914 1106 86-87 0.0806465729168302 1106 88-89 0.2515601179263314 1106 90-91 0.23309862955306215 1106 92-93 0.26651895477258947 1106 94-95 0.21814284875420853 1106 96-97 0.1883071562742984 1106 98-99 0.3064566161734632 1106 100-101 0.798976212391036 1107 1 -0.1307535889695366 1107 2 -0.10516552557751169 1107 3 -0.1607688242204901 1107 4 -0.12164619667660048 1107 5 -0.06401068437346424 1107 6 -0.08135275262126385 1107 7 -0.08984408599286553 1107 8 -0.06724048854777465 1107 9 -0.13819622271763876 1107 10-11 -0.12677702302863025 1107 12-13 -0.12990793653950305 1107 14-15 -0.11496706114387223 1107 16-17 -0.0451039794588155 1107 18-19 -0.10622001348301069 1107 20-21 -0.07531118995547104 1107 22-23 -0.09545413452455165 1107 24-25 -0.13282309862934483 1107 26-27 -0.14357169869515474 1107 28-29 -0.11014646190030675 1107 30-31 -0.17717830416954428 1107 32-33 -0.08789480482504075 1107 34-35 -0.11605456917499168 1107 36-37 -0.08109559156297763 1107 38-39 -0.051216288505834484 1107 40-41 -0.06982180722936704 1107 42-43 -0.1458402938879857 1107 44-45 -0.08277928934049328 1107 46-47 -0.13119280215030216 1107 48-49 -0.32997648137796887 1107 50-51 -0.2510315136707888 1107 52-53 -0.2339926665664862 1107 54-55 -0.25910883738858814 1107 56-57 -0.21526868818995837 1107 58-59 -0.21242451554359576 1107 60-61 -0.26675779347731066 1107 62-63 -0.2590036950672072 1107 64-65 -0.2991663256852135 1107 66-67 -0.28430520781264335 1107 68-69 -0.2307542469651338 1107 70-71 -0.27106075511986916 1107 72-73 -0.275831910587371 1107 74-75 -0.2194762655553646 1107 76-77 -0.3208092996913514 1107 78-79 -0.2680288294720192 1107 80-81 -0.2463716700444678 1107 82-83 -0.2160475011958205 1107 84-85 -0.28363313030708426 1107 86-87 -0.34702329047239644 1107 88-89 -0.23506131448812795 1107 90-91 -0.07729717400331992 1107 92-93 0.06873100324990489 1107 94-95 0.03172099917258109 1107 96-97 0.02186300619285575 1107 98-99 0.08796956967746894 1107 100-101 0.4902845608302826 1108 1 -0.155404681069367 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10608.0 13 14321.0 14 16577.0 15 20995.0 16 26542.0 17 35463.0 18 33463.0 19 24611.0 20 23787.0 21 25865.0 22 27977.0 23 31977.0 24 37623.0 25 45675.0 26 55634.0 27 69603.0 28 86746.0 29 106396.0 30 130905.0 31 160519.0 32 197387.0 33 249417.0 34 330033.0 35 459576.0 36 703642.0 37 1152345.0 38 1077800.0 39 122337.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.96939988955616 19.798357522228578 14.396694343345379 23.835548244869887 2 15.693700869027314 21.261495403726755 39.062780581507916 23.98202314573801 3 18.69810746799282 24.84032522388457 30.645170122439513 25.816397185683094 4 13.269910937115565 17.291194487228946 37.046511834296616 32.392382741358865 5 13.729472173017687 37.44204265886897 34.534125414635156 14.294359753478195 6 31.59622282885262 36.47084237094926 17.653133420985696 14.279801379212417 7 27.990504390076815 32.88755629096431 21.421297636426225 17.700641682532652 8 25.718246174744674 35.26219518757927 20.50055127332904 18.51900736434701 9 26.316272467055306 16.61393740578834 20.764093724207743 36.305696402948605 10-11 24.731883273938564 26.491190106498948 27.989607473374317 20.78731914618817 12-13 25.448434748176947 24.047516947477124 28.152412016720035 22.351636287625894 14-15 22.620676684843595 25.409292497017038 26.265585857153457 25.704444960985906 16-17 22.31684011689633 28.53555650242687 26.859181991930026 22.28842138874677 18-19 22.089833982371502 27.395405910613913 28.848299731481642 21.66646037553295 20-21 22.961284732155182 26.60800642607773 28.583451301482782 21.847257540284307 22-23 22.715057494248875 26.594835912393712 28.3535857124757 22.336520880881718 24-25 22.596994932515045 26.69035282059532 28.501180436792765 22.211471810096864 26-27 22.388368021216344 26.88887241917949 28.479220078484985 22.243539481119182 28-29 22.233366018866217 27.07375166661285 28.535282153944518 22.15760016057641 30-31 22.44685107651719 26.72315164822134 28.429719778674944 22.400277496586522 32-33 22.316505476573386 26.876524404348178 28.32520537976303 22.48176473931541 34-35 22.799785327407808 26.82043982199246 28.146913628272728 22.232861222327006 36-37 22.714936895136464 26.61897195012045 28.23292028926934 22.43317086547374 38-39 22.433750884744178 26.905174308766465 28.392875892581294 22.268198913908066 40-41 22.64567618768497 26.63746206221419 28.379909251456702 22.336952498644138 42-43 22.500443752903276 26.75019921672892 28.035949650096114 22.71340738027169 44-45 22.581914271487406 26.821179985463985 27.951054144492694 22.645851598555915 46-47 22.582471004078492 26.917843488585646 27.713950841709263 22.785734665626602 48-49 22.652942765929765 26.976498318799813 27.64433330645729 22.726225608813124 50-51 22.377689258675545 27.066631557789023 27.702802154834117 22.852877028701318 52-53 22.688216081773124 27.03429677293147 27.45087209571291 22.8266150495825 54-55 22.384340702967435 26.818413643254875 27.633833661793673 23.16341199198402 56-57 22.388381548742892 27.175386490848336 27.500447986289828 22.93578397411895 58-59 22.540527098221812 26.818439393032463 27.536107642495583 23.10492586625014 60-61 22.54916122707208 26.86098524308752 27.75295035339662 22.836903176443776 62-63 22.33331086320963 26.89402844188952 27.709033046376636 23.063627648524214 64-65 22.153077812150144 26.88955329960549 27.566583791563353 23.39078509668101 66-67 22.176222969682538 27.066043487940295 27.284940932150953 23.472792610226218 68-69 22.15929695490444 26.825956005582835 27.5122163441689 23.50253069534382 70-71 22.13836882958758 26.737281518602323 27.45336555247283 23.670984099337268 72-73 22.176510941156412 26.6024738662076 27.412625679272786 23.80838951336321 74-75 21.99911969985957 26.858623835212043 27.208090906189874 23.934165558738506 76-77 22.054288727435416 26.605088496725195 27.40436162532573 23.93626115051366 78-79 21.936404070885956 26.66338123651678 27.366846106056936 24.033368586540327 80-81 21.899188431849424 26.660108158733514 27.30308426109156 24.137619148325502 82-83 21.940697378227508 26.576047867783526 27.25767623799524 24.225578515993725 84-85 21.81254214328348 26.421589370385245 27.18192926216233 24.58393922416894 86-87 21.720739828996596 26.31633691167169 27.41886756271217 24.544055696619544 88-89 21.748577656502306 26.228020728140976 27.250313984523515 24.7730876308332 90-91 21.621568139002484 26.226841574893626 27.362819058721204 24.788771227382682 92-93 21.665145684763804 26.113786440545468 27.329996047901773 24.89107182678896 94-95 21.526983468495597 25.875105517579243 27.34036598184054 25.257545032084614 96-97 21.505730542430673 25.772976976053837 27.42696690528427 25.29432557623122 98-99 21.294124722439186 25.756774825259072 27.289645984454236 25.65945446784751 100-101 21.284502725323016 25.42980532435932 27.674642165635742 25.61104978468192 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 644.0 1 472.5 2 383.0 3 495.0 4 471.5 5 507.0 6 827.5 7 1607.0 8 2447.5 9 2864.5 10 2736.5 11 2593.5 12 2785.0 13 3114.0 14 3522.5 15 3853.0 16 4190.0 17 4569.0 18 5034.5 19 5616.5 20 6354.5 21 7395.0 22 8970.0 23 10935.5 24 13254.5 25 17009.5 26 23275.5 27 31232.5 28 38406.0 29 46779.0 30 56754.5 31 67156.5 32 77793.5 33 87634.0 34 98671.0 35 109725.0 36 122269.0 37 134544.5 38 142186.0 39 146791.5 40 151313.0 41 159699.5 42 168622.5 43 177531.5 44 186831.0 45 192903.5 46 196759.5 47 204271.0 48 219710.0 49 225146.0 50 218687.0 51 212307.5 52 202556.0 53 196563.5 54 190847.5 55 175487.5 56 157395.0 57 139293.5 58 124125.5 59 109848.0 60 92294.5 61 73764.5 62 57686.0 63 44811.0 64 33536.5 65 24699.5 66 17559.5 67 13240.0 68 9651.5 69 6414.5 70 4567.0 71 3343.0 72 2565.0 73 2030.5 74 1539.5 75 1125.0 76 728.5 77 384.5 78 206.0 79 118.0 80 72.0 81 49.0 82 25.0 83 11.5 84 11.5 85 14.0 86 11.0 87 5.5 88 3.0 89 2.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3935481665984066 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.6435439652517474E-4 16-17 0.0022186886851220024 18-19 1.4161842670991503E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.608859913129368E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007836219611281966 36-37 0.01746627262755619 38-39 0.011423886421266481 40-41 0.007722924869914035 42-43 0.0034177246979326167 44-45 0.002077070258412087 46-47 0.0016994211205189807 48-49 3.776491378931068E-5 50-51 2.737956249725024E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.776491378931068E-4 60-61 5.664737068396601E-4 62-63 0.0 64-65 0.0 66-67 0.001170712327468631 68-69 8.402693318121626E-4 70-71 0.0019637755170441554 72-73 0.0 74-75 0.0 76-77 0.10010534522701528 78-79 0.034743720686165824 80-81 1.888245689465534E-5 82-83 0.0 84-85 0.0 86-87 0.15354269823888989 88-89 0.32848866136787164 90-91 0.28502124559637504 92-93 0.0 94-95 4.720614223663835E-5 96-97 0.0 98-99 1.7938334049922574E-4 100-101 6.60885991312937E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5295921.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.50273972050647 #Duplication Level Percentage of deduplicated Percentage of total 1 70.61553191365041 32.83815700805603 2 15.311195186097942 14.240250490979683 3 5.7343823406874606 7.999934683405728 4 2.6685159729595096 4.963732149222007 5 1.480887408747753 3.4432660862186015 6 0.9256230692479671 2.582640520112073 7 0.625930565223467 2.037524031038747 8 0.43568549518815297 1.6208455346187718 9 0.32236703107363845 1.3491855126440846 >10 1.6540737418142535 14.117824377774 >50 0.1341268729960341 4.308716512871658 >100 0.08447925095269428 7.174980847121735 >500 0.005411038482671458 1.6338602482147704 >1k 0.0016273753435134132 1.0823761855112326 >5k 1.2205315076160529E-4 0.3922549458827048 >10k+ 4.068438358720177E-5 0.21445086632809815 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11335 0.21403264890091828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9513 0.17962881243885626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6188 0.11684464326412725 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.776491378931068E-5 2 0.0 0.0 0.0 0.0 3.776491378931068E-5 3 0.0 0.0 0.0 0.0 3.776491378931068E-5 4 0.0 0.0 0.0 0.0 3.776491378931068E-5 5 0.0 1.888245689465534E-5 0.0 1.888245689465534E-5 3.776491378931068E-5 6 0.0 3.776491378931068E-5 0.0 1.888245689465534E-5 3.776491378931068E-5 7 0.0 3.776491378931068E-5 0.0 1.888245689465534E-5 3.776491378931068E-5 8 0.0 3.776491378931068E-5 0.0 1.888245689465534E-5 3.776491378931068E-5 9 0.0 3.776491378931068E-5 0.0 1.888245689465534E-5 7.552982757862136E-5 10-11 9.44122844732767E-6 9.44122844732767E-5 0.0 4.720614223663835E-5 7.552982757862136E-5 12-13 1.888245689465534E-5 1.1329474136793204E-4 0.0 5.664737068396602E-5 7.552982757862136E-5 14-15 1.888245689465534E-5 1.1329474136793204E-4 0.0 7.552982757862136E-5 7.552982757862136E-5 16-17 1.888245689465534E-5 1.1329474136793204E-4 0.0 9.44122844732767E-5 7.552982757862136E-5 18-19 3.776491378931068E-5 1.1329474136793204E-4 0.0 1.227359698152597E-4 7.552982757862136E-5 20-21 5.664737068396602E-5 2.5491316807784705E-4 0.0 1.9826579739388105E-4 9.44122844732767E-5 22-23 5.664737068396602E-5 2.6435439652517474E-4 0.0 4.5317896547172816E-4 1.0385351292060437E-4 24-25 5.664737068396602E-5 2.737956249725024E-4 0.0 8.402693318121626E-4 1.1329474136793204E-4 26-27 5.664737068396602E-5 2.832368534198301E-4 0.0 0.0017371860343082912 1.1329474136793204E-4 28-29 7.552982757862136E-5 2.832368534198301E-4 0.0 0.006797684482075923 1.1329474136793204E-4 30-31 7.552982757862136E-5 3.021193103144855E-4 0.0 0.024556635191499268 1.1329474136793204E-4 32-33 7.552982757862136E-5 3.493254525511238E-4 0.0 0.06399264641598695 1.1329474136793204E-4 34-35 8.497105602594903E-5 3.587666809984514E-4 0.0 0.12433153742285809 1.1329474136793204E-4 36-37 9.44122844732767E-5 3.587666809984514E-4 0.0 0.22330393523619405 1.1329474136793204E-4 38-39 1.3217719826258737E-4 3.587666809984514E-4 0.0 0.36627245761407695 1.1329474136793204E-4 40-41 1.3217719826258737E-4 3.587666809984514E-4 0.0 0.5221754629647988 1.1329474136793204E-4 42-43 1.3217719826258737E-4 3.682079094457791E-4 0.0 0.686962664284456 1.1329474136793204E-4 44-45 1.3217719826258737E-4 3.776491378931068E-4 0.0 0.89122364174239 1.1329474136793204E-4 46-47 1.510596551572427E-4 3.870903663404344E-4 0.0 1.1328246777095052 1.1329474136793204E-4 48-49 1.510596551572427E-4 4.154140516824175E-4 0.0 1.3856418930720453 1.1329474136793204E-4 50-51 1.510596551572427E-4 4.342965085770728E-4 0.0 1.6477964833689929 1.227359698152597E-4 52-53 1.510596551572427E-4 4.531789654717281E-4 0.0 1.913925830842265 1.510596551572427E-4 54-55 1.510596551572427E-4 4.909438792610388E-4 0.0 2.221294464173465 1.7938334049922574E-4 56-57 1.510596551572427E-4 4.909438792610388E-4 0.0 2.5765962143317473 1.888245689465534E-4 58-59 1.510596551572427E-4 4.909438792610388E-4 0.0 2.9529141390137807 1.888245689465534E-4 60-61 1.510596551572427E-4 5.098263361556942E-4 0.0 3.3504087391031705 2.0770702584120874E-4 62-63 1.510596551572427E-4 5.098263361556942E-4 0.0 3.7542478447091643 2.2658948273586408E-4 64-65 1.510596551572427E-4 5.098263361556942E-4 0.0 4.186741078652798 2.2658948273586408E-4 66-67 1.510596551572427E-4 5.098263361556942E-4 0.0 4.671925808560966 2.2658948273586408E-4 68-69 1.510596551572427E-4 5.098263361556942E-4 0.0 5.202258492904256 2.2658948273586408E-4 70-71 1.6994211205189805E-4 5.098263361556942E-4 0.0 5.7746707324372855 2.2658948273586408E-4 72-73 1.9826579739388105E-4 5.098263361556942E-4 0.0 6.347262355310814 2.832368534198301E-4 74-75 2.0770702584120874E-4 5.192675646030218E-4 0.0 6.953899047965406 2.832368534198301E-4 76-77 2.0770702584120874E-4 5.287087930503495E-4 0.0 7.605107780119832 2.926780818671578E-4 78-79 2.0770702584120874E-4 5.287087930503495E-4 0.0 8.314663304078742 3.021193103144854E-4 80-81 2.454719396305194E-4 5.287087930503495E-4 0.0 9.060454262818498 3.021193103144854E-4 82-83 2.454719396305194E-4 5.287087930503495E-4 0.0 9.818887026449223 3.021193103144854E-4 84-85 2.5491316807784705E-4 5.287087930503495E-4 0.0 10.605152531542672 3.021193103144854E-4 86-87 2.6435439652517474E-4 5.475912499450048E-4 0.0 11.423414359844113 3.021193103144854E-4 88-89 2.6435439652517474E-4 5.664737068396602E-4 0.0 12.289061713722694 3.021193103144854E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15810 0.0 29.648634 1 GTATCAA 22550 0.0 28.328506 1 GTACATG 18830 0.0 24.13457 1 ATCAACG 26790 0.0 23.60989 3 TCAACGC 26875 0.0 23.517561 4 CAACGCA 27065 0.0 23.369997 5 TATCAAC 27560 0.0 23.070772 2 TACATGG 19270 0.0 22.875496 2 AACGCAG 27780 0.0 22.80266 6 ACATGGG 19380 0.0 21.79078 3 GAGTACT 13500 0.0 20.756565 12-13 ACGCAGA 30765 0.0 20.451405 7 CGCAGAG 30975 0.0 20.082968 8 AGAGTAC 24080 0.0 19.282867 10-11 GTACTTT 14970 0.0 18.686644 14-15 GCAGAGT 32665 0.0 18.681124 9 CATGGGG 9740 0.0 18.220032 4 AGTACTT 14475 0.0 18.211042 12-13 CATGGGA 10345 0.0 17.475555 4 GAGTACA 16080 0.0 16.589888 1 >>END_MODULE