FastQCFastQC Report
Thu 26 May 2016
SRR938764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5293978
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119380.2255015037841109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90700.17132674144093535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57480.10857619733213852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA213650.031.6354121
GGTATCA141650.030.7030351
TCAACGC252350.026.3563754
ATCAACG254000.026.2411943
CAACGCA254650.026.0065485
TATCAAC261200.025.6994742
GTACATG188650.025.4220851
AACGCAG261200.025.3352726
TACATGG194700.023.9756362
ACATGGG196300.022.813543
ACGCAGA290750.022.5649937
CGCAGAG292750.022.1839718
GAGTACT128500.021.3319512-13
GCAGAGT306750.020.2577259
AGAGTAC229000.020.03138710-11
CATGGGA98300.019.0144464
GTACTTT146600.018.7146514-15
AGTACTT139200.018.61808812-13
CATGGGG99900.018.4249884
GAGTACA158450.017.0685581
Produced by FastQC (version 0.11.5)