##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938764_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5293978 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.527177105760547 33.0 31.0 34.0 30.0 34.0 2 31.600254477823672 33.0 31.0 34.0 28.0 34.0 3 31.62245082998078 33.0 31.0 34.0 28.0 34.0 4 35.17437907751033 37.0 35.0 37.0 33.0 37.0 5 35.20761344304793 37.0 35.0 37.0 33.0 37.0 6 35.25446346773636 37.0 35.0 37.0 32.0 37.0 7 35.15919597701388 37.0 35.0 37.0 32.0 37.0 8 35.12748692948856 37.0 35.0 37.0 32.0 37.0 9 36.69939637074427 39.0 37.0 39.0 32.0 39.0 10-11 36.57918893882823 39.0 37.0 39.0 32.0 39.0 12-13 36.47601642847779 39.0 37.0 39.0 32.0 39.0 14-15 37.64881134753488 40.0 37.0 41.0 32.0 41.0 16-17 37.53340266997709 40.0 37.0 41.0 32.0 41.0 18-19 37.46383683498496 40.0 37.0 41.0 32.0 41.0 20-21 37.34875277154533 40.0 37.0 41.0 31.5 41.0 22-23 37.35631287096395 40.0 37.0 41.0 31.5 41.0 24-25 37.29697554466604 40.0 37.0 41.0 31.0 41.0 26-27 37.17445822404249 40.0 37.0 41.0 31.0 41.0 28-29 37.016879745250165 40.0 36.5 41.0 30.5 41.0 30-31 36.81185065370502 39.5 36.0 41.0 30.0 41.0 32-33 36.66067539381539 39.0 36.0 41.0 30.0 41.0 34-35 36.384980538264415 39.0 35.5 41.0 30.0 41.0 36-37 36.19451440863563 39.0 35.0 41.0 29.5 41.0 38-39 36.04769088575736 39.0 35.0 41.0 29.0 41.0 40-41 35.96673361695119 39.0 35.0 41.0 28.5 41.0 42-43 36.00303193553128 39.0 35.0 41.0 28.5 41.0 44-45 35.861812893820115 39.0 35.0 41.0 27.5 41.0 46-47 35.606988827683075 39.0 35.0 41.0 26.5 41.0 48-49 35.37043401011489 39.0 35.0 41.0 26.0 41.0 50-51 34.241581944617074 38.0 33.5 40.0 24.5 40.5 52-53 34.31903815996213 38.0 33.5 39.5 24.0 40.5 54-55 34.82893893778932 38.0 34.0 40.0 24.5 41.0 56-57 34.73904613883926 38.0 34.0 40.0 24.5 41.0 58-59 34.54088834521035 38.0 34.0 40.0 24.0 41.0 60-61 34.24584764046999 38.0 34.0 40.0 23.0 41.0 62-63 33.85305397944608 37.0 33.0 40.0 22.5 41.0 64-65 33.45913479428891 37.0 33.0 40.0 22.0 41.0 66-67 33.074543471846695 36.0 32.5 39.0 20.5 41.0 68-69 32.64253742648722 36.0 32.0 39.0 19.5 40.5 70-71 32.21781295653288 35.0 31.5 38.5 18.0 40.0 72-73 31.78367042703993 35.0 31.0 37.5 17.0 40.0 74-75 31.302965652671773 35.0 31.0 37.0 12.0 39.0 76-77 30.830019977415848 35.0 30.5 36.5 8.0 39.0 78-79 30.382288611701824 34.5 30.0 36.0 7.0 38.5 80-81 29.90569700138535 34.0 30.0 35.5 2.0 37.5 82-83 29.484578987672407 34.0 29.0 35.0 2.0 37.0 84-85 29.122966982484627 34.0 29.0 35.0 2.0 36.5 86-87 28.681236775067823 34.0 29.0 35.0 2.0 36.0 88-89 28.22221172811825 34.0 28.5 35.0 2.0 36.0 90-91 27.758715563230524 34.0 27.0 35.0 2.0 35.0 92-93 27.27985732090311 33.5 26.0 35.0 2.0 35.0 94-95 26.73921708779296 33.0 25.0 35.0 2.0 35.0 96-97 26.114319137707035 33.0 24.5 35.0 2.0 35.0 98-99 25.322800170306714 32.5 20.5 35.0 2.0 35.0 100-101 22.912706097380834 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21077194397117083 1101 2 0.2884360606871539 1101 3 0.4930287545322649 1101 4 0.22055766233140872 1101 5 0.20986401351398598 1101 6 0.3742536169010293 1101 7 0.36096806341300436 1101 8 0.3962567612995542 1101 9 0.5542369094329374 1101 10-11 0.46937951082341556 1101 12-13 0.42032920688387065 1101 14-15 0.5504888628179216 1101 16-17 0.5986333875015717 1101 18-19 0.6188201664112682 1101 20-21 0.6878074762303967 1101 22-23 0.6269952943012171 1101 24-25 0.6315030531239145 1101 26-27 0.562183218420806 1101 28-29 0.6419805870954676 1101 30-31 0.5378750963478964 1101 32-33 0.4593447692330912 1101 34-35 0.5596137840410762 1101 36-37 0.6321473045292763 1101 38-39 0.6733059172979594 1101 40-41 0.67841229444889 1101 42-43 0.7010661591646183 1101 44-45 0.6775583170689288 1101 46-47 0.6021646227461162 1101 48-49 0.5753801788093043 1101 50-51 0.599965638761276 1101 52-53 0.5749295781902433 1101 54-55 0.6269822574368575 1101 56-57 0.6450942042256784 1101 58-59 0.5267563870655678 1101 60-61 0.41849471535060445 1101 62-63 0.29516606562661707 1101 64-65 0.3024120099022056 1101 66-67 0.3263495128432581 1101 68-69 0.29375124536554864 1101 70-71 0.22021112289748856 1101 72-73 0.17777338537806386 1101 74-75 0.1434447885019985 1101 76-77 0.02531394291650102 1101 78-79 -0.045626173785365154 1101 80-81 0.06561521158040406 1101 82-83 0.12708796861786098 1101 84-85 0.12217783055623599 1101 86-87 -0.045557491163872754 1101 88-89 -0.09330420420000252 1101 90-91 -0.2443512855682961 1101 92-93 -0.2570750247760323 1101 94-95 -0.320435202638361 1101 96-97 -0.3844765560682397 1101 98-99 -0.478348106332696 1101 100-101 -0.1339857267473512 1102 1 0.20529919444796363 1102 2 0.22221240462103253 1102 3 0.2745151085965425 1102 4 0.396469022078044 1102 5 0.3702371750268867 1102 6 0.36460038367752645 1102 7 0.3531246797086496 1102 8 0.3601428236444235 1102 9 0.4882781415184567 1102 10-11 0.484120730264749 1102 12-13 0.4161657514388182 1102 14-15 0.5436369563307082 1102 16-17 0.6884023413608347 1102 18-19 0.6975259475133342 1102 20-21 0.5539080396492579 1102 22-23 0.5089762203974644 1102 24-25 0.44736928517180985 1102 26-27 0.5122393458079628 1102 28-29 0.6394565669069223 1102 30-31 0.6777332368699476 1102 32-33 0.6677129995138031 1102 34-35 0.7155847934835791 1102 36-37 0.7795787098250813 1102 38-39 0.7418166279764264 1102 40-41 0.7905600823938599 1102 42-43 0.8205990543669017 1102 44-45 0.8440481972050691 1102 46-47 0.8723531528144406 1102 48-49 0.8086001221513115 1102 50-51 0.8254471714773501 1102 52-53 0.7442730364049694 1102 54-55 0.5968832451885575 1102 56-57 0.7986476341866791 1102 58-59 0.6268480385918238 1102 60-61 0.5819252483481705 1102 62-63 0.5470444985605099 1102 64-65 0.5152756807499799 1102 66-67 0.47088679211567097 1102 68-69 0.5666211141219222 1102 70-71 0.595496934164963 1102 72-73 0.5111256034541292 1102 74-75 0.33946409045266535 1102 76-77 0.3274155118491393 1102 78-79 0.30358634937682893 1102 80-81 0.31776079055613593 1102 82-83 0.2665618538598409 1102 84-85 0.3008553127678226 1102 86-87 0.1497918383180057 1102 88-89 0.14215936699955378 1102 90-91 0.016339410569361945 1102 92-93 -0.038516390378124044 1102 94-95 -0.02474854436101026 1102 96-97 -0.32380413002543307 1102 98-99 -0.42892400004326703 1102 100-101 -0.026521593333132643 1103 1 0.1994465870043065 1103 2 0.3198081420065506 1103 3 0.40635032502170887 1103 4 0.2872074161654439 1103 5 0.3347185756351294 1103 6 0.28631687264253003 1103 7 0.3113236447975609 1103 8 0.350534784303008 1103 9 0.48037970516536177 1103 10-11 0.40428963815270436 1103 12-13 0.4826562092009894 1103 14-15 0.4817371496915186 1103 16-17 0.4887564367841932 1103 18-19 0.5455172849139345 1103 20-21 0.5667046796591961 1103 22-23 0.5691338414801592 1103 24-25 0.5095312614820315 1103 26-27 0.4838872568307906 1103 28-29 0.5053107700236623 1103 30-31 0.6407638768115902 1103 32-33 0.5213918860132694 1103 34-35 0.5424251349357974 1103 36-37 0.7018548298535876 1103 38-39 0.6709422147581208 1103 40-41 0.7227872860236815 1103 42-43 0.7401971491157724 1103 44-45 0.7880150346962722 1103 46-47 0.7787655440523906 1103 48-49 0.6336346584008155 1103 50-51 0.6915129794648536 1103 52-53 0.6914332201284665 1103 54-55 0.6519325486870713 1103 56-57 0.6235400035274026 1103 58-59 0.6251525450191906 1103 60-61 0.5822728199850005 1103 62-63 0.4579555933504338 1103 64-65 0.4141377891032363 1103 66-67 0.28687793838459896 1103 68-69 0.32063456104962285 1103 70-71 0.24324789875989694 1103 72-73 0.27174331149082676 1103 74-75 0.0497094390092343 1103 76-77 0.12406454017040858 1103 78-79 0.20765306843985343 1103 80-81 0.2156520765628258 1103 82-83 0.16464439881441706 1103 84-85 0.12586910308525034 1103 86-87 0.18491213763253 1103 88-89 0.2653883489612099 1103 90-91 0.21334639985751025 1103 92-93 0.04648440254921482 1103 94-95 -0.00344261757367903 1103 96-97 0.08333024146755363 1103 98-99 0.2394115474622467 1103 100-101 0.6805222633490935 1104 1 0.1246392245626069 1104 2 0.12514381487769555 1104 3 0.16112614703479977 1104 4 0.24907817738677096 1104 5 0.23794680278346192 1104 6 0.34408498556621225 1104 7 0.3408539844709111 1104 8 0.33800237104949815 1104 9 0.36187951553466746 1104 10-11 0.3058226138953657 1104 12-13 0.3669766438568516 1104 14-15 0.46776318087589885 1104 16-17 0.5612843568681924 1104 18-19 0.5034566483610163 1104 20-21 0.5289989129598638 1104 22-23 0.4615712697184975 1104 24-25 0.4128645540422795 1104 26-27 0.4572024357409745 1104 28-29 0.40515000093193976 1104 30-31 0.436461600644229 1104 32-33 0.4181363684021804 1104 34-35 0.4437312461521046 1104 36-37 0.5075135546536274 1104 38-39 0.5216781802125823 1104 40-41 0.5512305699458935 1104 42-43 0.4741156215844953 1104 44-45 0.5603787492711803 1104 46-47 0.6694543272812084 1104 48-49 0.5900146488240452 1104 50-51 0.718021567584799 1104 52-53 0.6822683524095581 1104 54-55 0.5998741615885663 1104 56-57 0.6000304607793154 1104 58-59 0.5652591621995313 1104 60-61 0.4599373841118677 1104 62-63 0.5315778760563177 1104 64-65 0.5758792829210435 1104 66-67 0.3612892698668375 1104 68-69 0.39951762927244516 1104 70-71 0.47086741461173176 1104 72-73 0.330667843425136 1104 74-75 0.25105045963451644 1104 76-77 0.3563385004021633 1104 78-79 0.21715391949121 1104 80-81 0.11202414887781842 1104 82-83 0.13712739437745824 1104 84-85 0.18456424160569185 1104 86-87 0.23800876757245604 1104 88-89 0.297555170451993 1104 90-91 0.21567457262220913 1104 92-93 0.23792466712322025 1104 94-95 0.24864081806601135 1104 96-97 0.0682525298302501 1104 98-99 0.08100273281495873 1104 100-101 0.5863385288738172 1105 1 0.05826625847289435 1105 2 0.0536087894649242 1105 3 0.21607106974267865 1105 4 0.2483604750192896 1105 5 0.18884821901097837 1105 6 0.1293589474731789 1105 7 0.11133286473279469 1105 8 0.10059974134294691 1105 9 0.20672778213877052 1105 10-11 0.19438332293911742 1105 12-13 0.22001831977187436 1105 14-15 0.2720774186287471 1105 16-17 0.333637867645173 1105 18-19 0.30412213680310884 1105 20-21 0.34227338792872786 1105 22-23 0.3083476223784274 1105 24-25 0.28064136975171294 1105 26-27 0.296682611386764 1105 28-29 0.3483168316750067 1105 30-31 0.42080665689010033 1105 32-33 0.48269662823853565 1105 34-35 0.41233051552580946 1105 36-37 0.5230877934095091 1105 38-39 0.5554634731291159 1105 40-41 0.5718231432218985 1105 42-43 0.5012731752001898 1105 44-45 0.5312366456631423 1105 46-47 0.6647863926134079 1105 48-49 0.6305502427366676 1105 50-51 0.7762878983220531 1105 52-53 0.7539113148884979 1105 54-55 0.6680270893697511 1105 56-57 0.5796658821665304 1105 58-59 0.5990735652576475 1105 60-61 0.6247351124496276 1105 62-63 0.6146145722938314 1105 64-65 0.5904945737932579 1105 66-67 0.4197426080488498 1105 68-69 0.5368322190597681 1105 70-71 0.2918680063440533 1105 72-73 0.4047488237350656 1105 74-75 0.3542232007449151 1105 76-77 0.31745561130165 1105 78-79 0.37894273038748594 1105 80-81 0.3908885584292001 1105 82-83 0.28193730654412974 1105 84-85 0.35884286829980994 1105 86-87 0.4003439761739216 1105 88-89 0.42941938183182415 1105 90-91 0.5290274397763959 1105 92-93 0.4676280295636843 1105 94-95 0.49399634192926456 1105 96-97 0.4278829402387281 1105 98-99 0.4769405325459459 1105 100-101 1.0060667570094068 1106 1 0.09145706853599123 1106 2 0.10093474305660877 1106 3 0.19429685661000562 1106 4 0.23025004433308283 1106 5 0.21303278863018704 1106 6 0.20575285664624943 1106 7 0.21184701323619493 1106 8 0.1563375735664465 1106 9 0.13578408927037344 1106 10-11 0.24149355698163077 1106 12-13 0.23313280340156695 1106 14-15 0.3502683311738508 1106 16-17 0.3904326174277415 1106 18-19 0.30597494720808527 1106 20-21 0.42941319496356556 1106 22-23 0.4250717522503962 1106 24-25 0.297433350829003 1106 26-27 0.25974305689735644 1106 28-29 0.20406892682321853 1106 30-31 0.13537659808572045 1106 32-33 0.26405629117217444 1106 34-35 0.29376566677267846 1106 36-37 0.4630912081234797 1106 38-39 0.5362327754458249 1106 40-41 0.6556483676355 1106 42-43 0.6462323322312287 1106 44-45 0.7015033281947893 1106 46-47 0.6607420907583759 1106 48-49 0.6163760511962408 1106 50-51 0.5909648503489109 1106 52-53 0.380444241071757 1106 54-55 0.3900987641347484 1106 56-57 0.5985456381687086 1106 58-59 0.6263068223529373 1106 60-61 0.6162509890769812 1106 62-63 0.5615209111136821 1106 64-65 0.5971051536026337 1106 66-67 0.6339355811925387 1106 68-69 0.7014545106345409 1106 70-71 0.6885392555009346 1106 72-73 0.6796139347541903 1106 74-75 0.4823330476557395 1106 76-77 0.5940960624172895 1106 78-79 0.6623492092660825 1106 80-81 0.5772694371674163 1106 82-83 0.651923661893683 1106 84-85 0.8287132600171567 1106 86-87 0.8241932672859313 1106 88-89 0.9589706939634013 1106 90-91 1.0278277576803099 1106 92-93 0.9991658691334102 1106 94-95 1.0267980078605206 1106 96-97 1.0492142281710208 1106 98-99 1.05128924925139 1106 100-101 1.3571723585946778 1107 1 -0.002616205518993553 1107 2 0.0011843012278838216 1107 3 0.03654953124933513 1107 4 0.06290924061863734 1107 5 0.04875851563927114 1107 6 0.02246338160964001 1107 7 0.0018981379722688985 1107 8 0.027310636755387918 1107 9 0.14443765038014078 1107 10-11 0.1046732185789736 1107 12-13 0.08921987652361452 1107 14-15 0.17567047037140782 1107 16-17 0.15705353243920683 1107 18-19 0.1326423739229412 1107 20-21 0.12233599340689949 1107 22-23 0.09892887035864106 1107 24-25 0.05401498609025879 1107 26-27 0.1125941944271247 1107 28-29 0.07531174288019571 1107 30-31 -0.002868289711152272 1107 32-33 0.13455163635035916 1107 34-35 0.1635939073774253 1107 36-37 0.1307584640303574 1107 38-39 -0.09557807452606681 1107 40-41 -0.0063705604044272945 1107 42-43 0.0305749913017479 1107 44-45 0.2889171364311096 1107 46-47 0.3374294416902117 1107 48-49 0.2869751896099402 1107 50-51 0.2550671996281082 1107 52-53 0.16233783080910058 1107 54-55 0.218997767389709 1107 56-57 0.34742389089095127 1107 58-59 0.4525355139556737 1107 60-61 0.43662832392608664 1107 62-63 0.3603798718654474 1107 64-65 0.2840554203497945 1107 66-67 0.09000433945231379 1107 68-69 0.36456541627117645 1107 70-71 0.33657133589077404 1107 72-73 0.32475298029595834 1107 74-75 0.3075201723312304 1107 76-77 0.22807710692365646 1107 78-79 0.2333819673430142 1107 80-81 0.22660181530140022 1107 82-83 7.769391890164457E-4 1107 84-85 0.11530403546646184 1107 86-87 0.006511905858403111 1107 88-89 0.12110797381314597 1107 90-91 0.17909176718064046 1107 92-93 0.1900680314569847 1107 94-95 0.1480412972571159 1107 96-97 0.35670727548821546 1107 98-99 0.6810176753782216 1107 100-101 1.1229912841461989 1108 1 0.24069979872364655 1108 2 0.3297017159298008 1108 3 0.20874243114855418 1108 4 0.2888356086457762 1108 5 0.34757501229009335 1108 6 0.27338370226677 1108 7 0.2119658886264233 1108 8 0.3241865883902193 1108 9 0.3384148002273619 1108 10-11 0.3939577816686963 1108 12-13 0.47194837230568254 1108 14-15 0.6444430941428223 1108 16-17 0.6511786776622728 1108 18-19 0.7033815205456619 1108 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-2.1921771730831026 2316 50-51 -2.3977922455468743 2316 52-53 -2.62664618779651 2316 54-55 -2.4917609446009052 2316 56-57 -2.173162947662213 2316 58-59 -1.981669550173386 2316 60-61 -1.8086557648063177 2316 62-63 -2.216578771242208 2316 64-65 -2.3772795800805717 2316 66-67 -2.139895359218926 2316 68-69 -2.2301769840898302 2316 70-71 -2.0478835751235813 2316 72-73 -1.9520996828054145 2316 74-75 -1.8998528357471542 2316 76-77 -1.8316864807318396 2316 78-79 -1.7402544721666011 2316 80-81 -1.7872010372592086 2316 82-83 -1.7434896705851486 2316 84-85 -1.7847730069792753 2316 86-87 -1.863241007923211 2316 88-89 -1.830616550561075 2316 90-91 -2.0002693806647294 2316 92-93 -1.7487754848129775 2316 94-95 -1.758601253531939 2316 96-97 -1.9915638840690058 2316 98-99 -2.2391909972080555 2316 100-101 -2.3169465638446027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24851.0 3 5501.0 4 5174.0 5 6145.0 6 7021.0 7 8626.0 8 10708.0 9 12714.0 10 14841.0 11 18407.0 12 22374.0 13 26551.0 14 27951.0 15 30198.0 16 35328.0 17 42383.0 18 39611.0 19 32339.0 20 34220.0 21 37921.0 22 42154.0 23 48016.0 24 55061.0 25 64499.0 26 73564.0 27 85953.0 28 100292.0 29 118078.0 30 139862.0 31 168806.0 32 206229.0 33 258302.0 34 338452.0 35 462427.0 36 680630.0 37 992764.0 38 880161.0 39 135841.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23677527948141 19.061713783910644 13.904666616912047 23.796844319695897 2 15.382279251303771 20.713066194475658 39.88179834989225 24.02285620432832 3 18.784520975390386 24.693671591416255 30.40264739517226 26.1191600380211 4 12.907231015469476 16.678262261513744 37.523881028250806 32.89062569476597 5 13.811026833619291 37.20538092544752 34.51096069208322 14.472631548849964 6 31.586389799977706 35.96218392042489 18.04364683768533 14.407779441912073 7 28.04820593964013 32.801728333250644 21.550273569445967 17.59979215766326 8 25.7454300439952 34.78698157170733 20.777108406355673 18.690479977941802 9 26.582536151742158 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22.519233648252442 34-35 22.795877214293913 26.74722301188809 28.169427295345383 22.287472478472615 36-37 22.746251995405046 26.554898696863244 28.186640757299003 22.512208550432707 38-39 22.491139425803127 26.796994984720175 28.383474596558617 22.32839099291808 40-41 22.665882582624988 26.553268275207813 28.393625984330324 22.387223157836875 42-43 22.43994380398554 26.66400204245721 28.09809255315589 22.79796160040136 44-45 22.561827304515354 26.748922928723672 27.98876849794067 22.7004812688203 46-47 22.56043757446782 26.836348203413795 27.763726778793576 22.83948744332481 48-49 22.70329782050268 26.85888510234573 27.649194004983517 22.788623072168075 50-51 22.4237844241169 27.017026819913198 27.72919057765405 22.82999817831585 52-53 22.701456694600903 26.96997812473566 27.535768731382603 22.792796449280832 54-55 22.40198326318253 26.728186589381302 27.73325474319192 23.136575404244248 56-57 22.468600647293616 27.087263101182785 27.570349026428804 22.873787225094798 58-59 22.621370937206393 26.689820459848967 27.640744167866643 23.048064435077997 60-61 22.58004091759793 26.649032918361975 27.936649636459087 22.834276527581007 62-63 22.409946660411574 26.702381252620377 27.911647690586772 22.976024396381277 64-65 22.304207658726277 26.648878320293996 27.796436267019438 23.25047775396029 66-67 22.25539127531547 26.828281621100714 27.56255359026308 23.35377351332074 68-69 22.232061790465004 26.580929626724974 27.814853220327535 23.372155362482484 70-71 22.165853288372247 26.537825647738426 27.75658409572727 23.53973696816205 72-73 22.28630455448779 26.381859860703127 27.755555831663486 23.5762797531456 74-75 22.0733292791694 26.64723804075383 27.614006289138775 23.665426390937995 76-77 22.180684023004517 26.393339541937046 27.74258715570057 23.683389279357865 78-79 22.076408594874348 26.43934340069885 27.743029725724284 23.74121827870252 80-81 22.062031238237402 26.45777524693279 27.68154056402826 23.79865295080155 82-83 22.12288131736091 26.33952818538208 27.709560318356154 23.828030178900864 84-85 22.039213641093962 26.188776393113077 27.662926728664456 24.109083237128505 86-87 22.00250032830439 26.0605322181572 27.945933363453584 23.991034090084828 88-89 22.05353491226122 25.920872425568803 27.785389636836094 24.240203025333884 90-91 21.96656676214504 25.926598630422827 27.978441489462604 24.128393117969534 92-93 22.091453229134615 25.7528374978802 27.927411574434313 24.228297698550872 94-95 21.961953509610556 25.556956337681218 27.995793796228853 24.485296356479374 96-97 22.07738042164732 25.347006296443148 28.022567787783874 24.55304549412566 98-99 21.90101795362108 25.329535451951678 27.89262163843866 24.87682495598859 100-101 22.163151217525947 24.82696009715439 28.30658101144458 24.703307673875077 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4309.0 1 2874.0 2 1301.5 3 1203.5 4 1213.0 5 1281.0 6 1609.0 7 2390.5 8 3398.5 9 3907.0 10 3747.5 11 3576.0 12 3860.0 13 4200.0 14 4497.0 15 4837.0 16 5169.0 17 5521.5 18 5838.0 19 6380.5 20 7113.0 21 8067.5 22 9502.0 23 11449.5 24 13865.0 25 17666.0 26 23989.5 27 31740.5 28 38916.0 29 47136.5 30 56859.5 31 67195.5 32 77482.5 33 87329.5 34 98551.5 35 110074.0 36 122852.0 37 135331.0 38 142811.5 39 147543.5 40 151714.0 41 159635.0 42 168730.5 43 177323.5 44 186318.5 45 192076.0 46 196093.0 47 203028.5 48 217248.0 49 222441.5 50 214867.0 51 207110.0 52 197528.5 53 192217.5 54 186747.0 55 171587.5 56 154344.5 57 136598.5 58 121423.5 59 107652.5 60 91106.5 61 73738.0 62 58603.0 63 46451.5 64 34912.0 65 25972.0 66 19217.5 67 14530.5 68 10858.0 69 7525.0 70 5512.5 71 4127.5 72 3170.5 73 2516.5 74 1953.0 75 1483.5 76 1012.0 77 590.5 78 380.0 79 278.0 80 201.5 81 153.5 82 122.0 83 93.5 84 81.5 85 65.0 86 50.5 87 39.0 88 25.0 89 21.5 90 15.0 91 9.5 92 9.5 93 8.5 94 5.5 95 3.5 96 4.0 97 4.5 98 3.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27835400902686036 2 0.1721012063140421 3 0.20015194623022614 4 0.16858778030433827 5 0.2432764170912686 6 0.20054862336035395 7 0.2465442810680362 8 0.2531555665701671 9 0.15232401796909623 10-11 0.21157058076176366 12-13 0.21663293651768106 14-15 0.17784358000732153 16-17 0.2919827018548245 18-19 0.23795905460884045 20-21 0.20290035206039767 22-23 0.24901879078454803 24-25 0.2375340433979892 26-27 0.34906642981893765 28-29 0.3201751121746256 30-31 0.29591169438180515 32-33 0.19643073696188387 34-35 0.363497921600732 36-37 0.40119169365645263 38-39 0.3814617287793791 40-41 0.3434657265292753 42-43 0.3766260456692491 44-45 0.38568350680716845 46-47 0.39152032743619264 48-49 0.41176975045986214 50-51 0.40394009948662424 52-53 0.4034017519528793 54-55 0.3976593782595999 56-57 0.3752282310202271 58-59 0.349992009789236 60-61 0.317086697375773 62-63 0.38551350232282794 64-65 0.41886271533429115 66-67 0.424973432076975 68-69 0.42962022131561556 70-71 0.4297902257999561 72-73 0.42099721608212204 74-75 0.4161709776655664 76-77 0.400218890218282 78-79 0.3729048364009069 80-81 0.38490904193406167 82-83 0.3945992975414707 84-85 0.39555321159249246 86-87 0.39045307706227717 88-89 0.3992933102479837 90-91 0.420817766904207 92-93 0.4204022003869302 94-95 0.42910076316901963 96-97 0.4296957788642114 98-99 0.4272401585348484 100-101 0.39919886331223886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5293978.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.74275327388515 #Duplication Level Percentage of deduplicated Percentage of total 1 72.67694308012547 35.4247430525475 2 14.273200331453111 13.914301643695095 3 5.316579578411913 7.774341799545245 4 2.529446595203483 4.931687652378888 5 1.382817936093691 3.370117674085893 6 0.8718528997910224 2.549790647138107 7 0.5750802640677501 1.9621696796894543 8 0.3760701099861268 1.4664554067789302 9 0.2832628864273997 1.2426311686301366 >10 1.5105813842486384 13.473545703312178 >50 0.12302767989234449 4.131798878929468 >100 0.07500049220089897 6.74428566885959 >500 0.004621995243181039 1.4848793544757681 >1k 0.0013982463278211944 1.0227132037820794 >5k 7.768035154399904E-5 0.2805309833846562 >10k+ 3.884017577199952E-5 0.22600748276731178 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11938 0.2255015037841109 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9070 0.17132674144093535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5748 0.10857619733213852 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.8889387148945462E-5 0.0 10-11 0.0 0.0 0.0 3.7778774297890924E-5 0.0 12-13 0.0 0.0 0.0 3.7778774297890924E-5 0.0 14-15 9.444693574472731E-6 0.0 0.0 3.7778774297890924E-5 3.7778774297890924E-5 16-17 3.7778774297890924E-5 0.0 0.0 5.666816144683639E-5 5.666816144683639E-5 18-19 3.7778774297890924E-5 0.0 0.0 1.1333632289367278E-4 5.666816144683639E-5 20-21 3.7778774297890924E-5 0.0 0.0 1.9833856506392734E-4 5.666816144683639E-5 22-23 3.7778774297890924E-5 0.0 0.0 4.155665172768002E-4 8.500224217025459E-5 24-25 7.555754859578185E-5 2.8334080723418195E-5 0.0 9.539140510217458E-4 1.1333632289367278E-4 26-27 7.555754859578185E-5 5.666816144683639E-5 0.0 0.001992830344213746 1.227810164681455E-4 28-29 8.500224217025458E-5 5.666816144683639E-5 0.0 0.006649064276428803 1.3222571004261824E-4 30-31 9.44469357447273E-5 8.500224217025459E-5 0.0 0.023290614354649755 1.3222571004261824E-4 32-33 1.1333632289367278E-4 1.1333632289367278E-4 0.0 0.061494399863391946 1.511150971915637E-4 34-35 1.511150971915637E-4 1.1333632289367278E-4 0.0 0.11946592902350558 1.7000448434050915E-4 36-37 1.7000448434050915E-4 1.227810164681455E-4 0.0 0.21021998958061405 1.7000448434050915E-4 38-39 1.7000448434050915E-4 1.511150971915637E-4 0.0 0.34422130201523315 1.7000448434050915E-4 40-41 1.7000448434050915E-4 1.511150971915637E-4 0.0 0.49014181774083687 1.7000448434050915E-4 42-43 1.7000448434050915E-4 1.7000448434050915E-4 0.0 0.6493699067128726 2.077832586384001E-4 44-45 1.7000448434050915E-4 1.888938714894546E-4 0.0 0.8442233798478196 2.45562032936291E-4 46-47 1.888938714894546E-4 1.888938714894546E-4 0.0 1.0754767020187845 2.644514200852365E-4 48-49 1.888938714894546E-4 1.888938714894546E-4 0.0 1.3186208934000105 2.644514200852365E-4 50-51 1.888938714894546E-4 1.888938714894546E-4 0.0 1.5696136251416233 2.644514200852365E-4 52-53 1.888938714894546E-4 1.888938714894546E-4 0.0 1.826462066899409 2.927855008086547E-4 54-55 1.888938714894546E-4 2.0778325863840007E-4 0.0 2.1183503218184887 3.2111958153207285E-4 56-57 1.888938714894546E-4 2.172279522128728E-4 0.0 2.454996979586995 3.2111958153207285E-4 58-59 1.888938714894546E-4 2.2667264578734556E-4 0.0 2.8184665671070035 3.2111958153207285E-4 60-61 1.888938714894546E-4 2.2667264578734556E-4 0.0 3.2036211710740012 3.2111958153207285E-4 62-63 1.888938714894546E-4 2.2667264578734556E-4 0.0 3.5960670784804925 3.2111958153207285E-4 64-65 1.888938714894546E-4 2.45562032936291E-4 0.0 4.0213521854454255 3.2111958153207285E-4 66-67 1.888938714894546E-4 2.45562032936291E-4 0.0 4.500623160882044 3.305642751065456E-4 68-69 1.888938714894546E-4 2.45562032936291E-4 0.0 5.023793072052811 3.400089686810183E-4 70-71 1.888938714894546E-4 2.45562032936291E-4 0.0 5.5767043232895945 3.400089686810183E-4 72-73 1.9833856506392734E-4 2.644514200852365E-4 0.0 6.138682480357871 3.872324365533819E-4 74-75 2.0778325863840007E-4 2.644514200852365E-4 0.0 6.729929742813439 3.966771301278547E-4 76-77 2.0778325863840007E-4 2.644514200852365E-4 0.0 7.373576165220181 4.0612182370232744E-4 78-79 2.0778325863840007E-4 2.644514200852365E-4 0.0 8.072776275231972 4.1556651727680014E-4 80-81 2.2667264578734556E-4 2.644514200852365E-4 0.0 8.808310121424759 4.4390059800021836E-4 82-83 2.2667264578734556E-4 2.644514200852365E-4 0.0 9.549605230697974 4.816793722981093E-4 84-85 2.2667264578734556E-4 2.738961136597092E-4 0.0 10.318629960305842 4.91124065872582E-4 86-87 2.2667264578734556E-4 2.8334080723418193E-4 0.0 11.121221130877386 4.91124065872582E-4 88-89 2.2667264578734556E-4 2.8334080723418193E-4 0.0 11.961572186359671 5.005687594470548E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 21365 0.0 31.635412 1 GGTATCA 14165 0.0 30.703035 1 TCAACGC 25235 0.0 26.356375 4 ATCAACG 25400 0.0 26.241194 3 CAACGCA 25465 0.0 26.006548 5 TATCAAC 26120 0.0 25.699474 2 GTACATG 18865 0.0 25.422085 1 AACGCAG 26120 0.0 25.335272 6 TACATGG 19470 0.0 23.975636 2 ACATGGG 19630 0.0 22.81354 3 ACGCAGA 29075 0.0 22.564993 7 CGCAGAG 29275 0.0 22.183971 8 GAGTACT 12850 0.0 21.33195 12-13 GCAGAGT 30675 0.0 20.257725 9 AGAGTAC 22900 0.0 20.031387 10-11 CATGGGA 9830 0.0 19.014446 4 GTACTTT 14660 0.0 18.71465 14-15 AGTACTT 13920 0.0 18.618088 12-13 CATGGGG 9990 0.0 18.424988 4 GAGTACA 15845 0.0 17.068558 1 >>END_MODULE