FastQCFastQC Report
Thu 26 May 2016
SRR938764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5293978
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113850.2150556726907441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94020.17759801797438524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61770.11667974441903611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA219600.028.9634191
GGTATCA151100.028.3668481
GTACATG193250.024.912181
ATCAACG263050.023.8163813
TACATGG200350.023.6181552
TCAACGC265400.023.5876164
CAACGCA267850.023.3718595
TATCAAC273100.023.1137312
AACGCAG275400.022.8614376
ACATGGG199550.022.476063
GAGTACT136900.020.17142712-13
ACGCAGA309200.020.1310447
CGCAGAG311900.019.8046678
AGAGTAC242150.019.339710-11
CATGGGG103400.019.2324074
AGTACTT146700.018.6945412-13
GCAGAGT327300.018.4959499
GTACTTT151850.017.95114914-15
GAGTACA162400.017.517251
AGTACAT150950.017.0414412
Produced by FastQC (version 0.11.5)