FastQCFastQC Report
Thu 26 May 2016
SRR938763_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938763_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026282
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58610.2892489791647954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.24572098059401407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.15555584069739553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.043.809781
GTATCAA68600.036.0024951
ATCAACG84550.029.0267853
TCAACGC85550.028.6874894
TATCAAC85650.028.6539942
CAACGCA86000.028.5373825
AACGCAG89800.027.3088676
ACGCAGA99650.024.3725557
CGCAGAG99550.024.1110978
TACATGG58050.022.1182042
GCAGAGT108800.021.5836289
GTACATG60250.021.5581991
ACATGGG57650.021.3676533
GAGTACT62300.021.12810912-13
AGAGTAC90000.019.96369710-11
CATGGGG37000.019.7197694
CAGAGTA101950.019.6902110-11
GTACTTT68550.019.0295614-15
TGGTATC25300.018.5394782
AGTACTT67750.018.27537312-13
Produced by FastQC (version 0.11.5)