FastQCFastQC Report
Thu 26 May 2016
SRR938763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026282
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.2673862769347998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50910.25124834549189107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32360.1597013643708033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.045.956521
GTATCAA68650.033.238941
ATCAACG85050.026.500463
TCAACGC85400.026.391854
CAACGCA86700.026.0508545
TATCAAC86850.026.0058612
AACGCAG90500.024.9045776
ACGCAGA100600.022.2627167
CGCAGAG101450.021.7955578
GAGTACT62650.020.67796712-13
GCAGAGT110150.019.9017649
AGAGTAC92300.019.8707410-11
GTGGTAT24600.019.3649831
CAGAGTA105450.018.6078110-11
GTACTTT67900.018.58994714-15
GTACATG60300.018.5653321
TACATGG58500.018.4932442
TGGTATC24450.018.4365162
ACATGGG58150.018.114963
AGTACTT68600.017.77768912-13
Produced by FastQC (version 0.11.5)