##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938763_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2026282 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.960704877208602 31.0 31.0 34.0 30.0 34.0 2 32.18635165292886 34.0 31.0 34.0 30.0 34.0 3 32.27336125968646 34.0 31.0 34.0 30.0 34.0 4 35.922444161276665 37.0 35.0 37.0 35.0 37.0 5 35.81906713872995 37.0 35.0 37.0 35.0 37.0 6 35.83516114736251 37.0 35.0 37.0 35.0 37.0 7 35.76232577696491 37.0 35.0 37.0 33.0 37.0 8 35.731948465218565 37.0 35.0 37.0 33.0 37.0 9 37.37801549833636 39.0 37.0 39.0 34.0 39.0 10-11 37.308648549412176 39.0 37.0 39.0 34.0 39.0 12-13 37.24270881348203 39.0 37.0 39.0 33.0 39.0 14-15 38.47439250805169 40.0 38.0 41.0 33.0 41.0 16-17 38.40284002917659 40.0 38.0 41.0 33.0 41.0 18-19 38.40792002297805 40.0 38.0 41.0 33.5 41.0 20-21 38.45740499101309 40.0 38.0 41.0 34.0 41.0 22-23 38.410305673149146 40.0 38.0 41.0 34.0 41.0 24-25 38.33774913857005 40.0 38.0 41.0 33.0 41.0 26-27 38.172622813606395 40.0 38.0 41.0 33.0 41.0 28-29 38.00863453359404 40.0 37.0 41.0 33.0 41.0 30-31 37.79202820732751 40.0 37.0 41.0 32.5 41.0 32-33 37.57377428215816 40.0 36.5 41.0 32.0 41.0 34-35 37.5494185903048 40.0 37.0 41.0 32.0 41.0 36-37 37.47099095782325 40.0 37.0 41.0 32.0 41.0 38-39 37.37154453328806 40.0 36.5 41.0 31.0 41.0 40-41 37.22084389043579 40.0 36.0 41.0 31.0 41.0 42-43 37.06050490504283 40.0 36.0 41.0 31.0 41.0 44-45 36.828551751434404 40.0 36.0 41.0 30.5 41.0 46-47 36.56016314609714 39.0 35.5 41.0 30.0 41.0 48-49 36.303656154474055 39.0 35.0 41.0 30.0 41.0 50-51 36.068335996667784 39.0 35.0 41.0 29.0 41.0 52-53 35.85936483668117 39.0 35.0 40.5 28.5 41.0 54-55 35.61722257810117 38.5 35.0 40.0 28.0 41.0 56-57 35.34331943924883 38.0 34.5 40.0 28.0 41.0 58-59 35.02413237644119 38.0 34.0 40.0 27.0 41.0 60-61 34.6886351455523 37.5 34.0 40.0 26.0 41.0 62-63 34.30721488914179 37.0 33.5 40.0 26.0 41.0 64-65 33.93728760360108 36.5 33.0 39.5 26.0 41.0 66-67 33.53631404710697 36.0 33.0 39.0 25.0 40.5 68-69 33.087995155659485 35.5 32.5 39.0 23.5 40.0 70-71 32.6269189086218 35.0 32.0 38.0 23.0 40.0 72-73 32.29685182023036 35.0 32.0 37.0 22.0 39.5 74-75 31.88988131957941 35.0 31.0 37.0 21.5 39.0 76-77 29.81913894512215 33.0 28.5 35.0 18.5 37.5 78-79 30.890669215834716 34.0 30.5 36.0 20.0 38.0 80-81 30.952471324327018 34.0 31.0 35.5 20.0 37.0 82-83 30.66621748601626 34.0 31.0 35.0 18.0 37.0 84-85 30.297635521610516 34.0 30.5 35.0 16.5 36.5 86-87 29.78023690680764 34.0 30.0 35.0 8.5 36.0 88-89 29.284618083761295 34.0 29.0 35.0 4.0 35.5 90-91 28.892879668279143 34.0 29.0 35.0 2.0 35.0 92-93 28.62683550463361 34.0 29.0 35.0 2.0 35.0 94-95 28.26259449573159 34.0 29.0 35.0 2.0 35.0 96-97 27.77785816584266 33.0 28.5 35.0 2.0 35.0 98-99 27.06651862870025 33.0 27.0 35.0 2.0 35.0 100-101 24.080874231720955 30.0 19.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1366276334147969 1101 2 0.19274054700866117 1101 3 0.19884963759351848 1101 4 0.10970367296638273 1101 5 0.0662592883160329 1101 6 0.15043648194592407 1101 7 0.16901147520845683 1101 8 0.18526646938138214 1101 9 0.30647415032413505 1101 10-11 0.2716677180969711 1101 12-13 0.3172473578010866 1101 14-15 0.39428777315279007 1101 16-17 0.28492526872084767 1101 18-19 0.3400758378976434 1101 20-21 0.3604676779032374 1101 22-23 0.27115410273422214 1101 24-25 0.3023302732435411 1101 26-27 0.34173606908263565 1101 28-29 0.3861503474836212 1101 30-31 0.3936962251794611 1101 32-33 0.35756688420117655 1101 34-35 0.35629776384347167 1101 36-37 0.240604603013999 1101 38-39 0.2859761488254833 1101 40-41 0.48725940902072296 1101 42-43 0.30496841789951645 1101 44-45 0.45680482895514274 1101 46-47 0.5772283813609533 1101 48-49 0.5008577586106213 1101 50-51 0.5471151378419918 1101 52-53 0.5819432384816068 1101 54-55 0.4523570564311612 1101 56-57 0.4837562257081487 1101 58-59 0.4626113853446796 1101 60-61 0.5125694071962954 1101 62-63 0.3932717883104928 1101 64-65 0.4055978109942515 1101 66-67 0.277382960858354 1101 68-69 0.42457645782425146 1101 70-71 0.3728258329426595 1101 72-73 0.39253067506778905 1101 74-75 0.456792087600558 1101 76-77 0.27255408538928094 1101 78-79 0.5187787391208474 1101 80-81 0.4834776495317392 1101 82-83 0.44365455256631847 1101 84-85 0.4747598160280333 1101 86-87 0.5642090279487171 1101 88-89 0.7995545418343433 1101 90-91 0.8506081306014401 1101 92-93 0.8353283156977618 1101 94-95 0.6979485783797124 1101 96-97 0.36651819265387786 1101 98-99 0.6151016745845759 1101 100-101 1.2293520281192123 1102 1 0.13790363869348 1102 2 0.16517301091178638 1102 3 0.23743545501316987 1102 4 0.18989793627952878 1102 5 0.17571919361024158 1102 6 0.18770748023811734 1102 7 0.20736926126652122 1102 8 0.16122614179250405 1102 9 0.23075318308302428 1102 10-11 0.27856202798673735 1102 12-13 0.2795421295756526 1102 14-15 0.4086561747984945 1102 16-17 0.47283281413304934 1102 18-19 0.3289750771462039 1102 20-21 0.3520353692555105 1102 22-23 0.28420283352136977 1102 24-25 0.18587902343761442 1102 26-27 0.20325796009335306 1102 28-29 0.35426477070983253 1102 30-31 0.25343995674288067 1102 32-33 0.3884990958142822 1102 34-35 0.4354343497773314 1102 36-37 0.37121320416289194 1102 38-39 0.4480530778737659 1102 40-41 0.49019470633480466 1102 42-43 0.39998229385045647 1102 44-45 0.3964856820835436 1102 46-47 0.47278031429062395 1102 48-49 0.5967740565457191 1102 50-51 0.6952967090266 1102 52-53 0.6628240217450738 1102 54-55 0.7239302787568889 1102 56-57 0.5806477215938841 1102 58-59 0.4338915631121054 1102 60-61 0.404759090475288 1102 62-63 0.5721720949398943 1102 64-65 0.5832002795550295 1102 66-67 0.6590055738066596 1102 68-69 0.7830587393022768 1102 70-71 0.6493211364669591 1102 72-73 0.7937761144405613 1102 74-75 0.8836619447655885 1102 76-77 0.6964279986015178 1102 78-79 0.5870110594127276 1102 80-81 0.6876457717177331 1102 82-83 0.774595982468508 1102 84-85 0.7967740607105327 1102 86-87 0.7411856231979002 1102 88-89 0.868606804681729 1102 90-91 1.0459558056518965 1102 92-93 0.9773845763715734 1102 94-95 0.8491503521726003 1102 96-97 1.0138968023418116 1102 98-99 1.095825746933592 1102 100-101 1.5677796791050866 1103 1 0.03584156566140706 1103 2 0.06249456533345921 1103 3 -0.08615132225404665 1103 4 0.005967258956872001 1103 5 0.021322128706422916 1103 6 0.10862327065147781 1103 7 0.10449994281774622 1103 8 0.0659532699369123 1103 9 0.1848958291330689 1103 10-11 0.0795124574213304 1103 12-13 0.0720951804876151 1103 14-15 0.047366573082634034 1103 16-17 -0.0653740615188596 1103 18-19 0.07351787495486661 1103 20-21 0.13371850384613282 1103 22-23 -0.005651862303622579 1103 24-25 0.11225201604231216 1103 26-27 0.04706346005107065 1103 28-29 0.043056539566890706 1103 30-31 0.048881061009971916 1103 32-33 0.20646554799856887 1103 34-35 0.045885432290795336 1103 36-37 0.01676874405708162 1103 38-39 0.1962121824509495 1103 40-41 0.12306835906598934 1103 42-43 0.21613985852405904 1103 44-45 0.1525632211038257 1103 46-47 0.18581558054946612 1103 48-49 0.19147533756697044 1103 50-51 0.29554871994569254 1103 52-53 0.2648257495850288 1103 54-55 0.19789213589705668 1103 56-57 0.27203873847057025 1103 58-59 -0.0038693454192610943 1103 60-61 0.1684640562238826 1103 62-63 0.02846646554100829 1103 64-65 -0.015838073558605004 1103 66-67 0.02725572180654723 1103 68-69 0.14320692125286882 1103 70-71 0.11681078822088153 1103 72-73 0.21262739452212998 1103 74-75 0.16503146054754936 1103 76-77 0.10579642082875296 1103 78-79 0.19152878533572704 1103 80-81 0.3029660211562728 1103 82-83 0.2884789004624757 1103 84-85 0.08109300281673626 1103 86-87 0.14195786546667222 1103 88-89 0.2802430902604307 1103 90-91 0.4728475319883607 1103 92-93 0.44186709946425395 1103 94-95 0.17442253688585652 1103 96-97 0.07921893114399836 1103 98-99 0.5041483326560652 1103 100-101 0.9036701216012553 1104 1 0.0012828357068990215 1104 2 -0.07515439048989236 1104 3 -0.03611391285820531 1104 4 -0.0223343824415565 1104 5 -0.01203474574205643 1104 6 -0.053280678801783665 1104 7 -0.008821018610717601 1104 8 -0.11306586269652996 1104 9 -0.10675397417042376 1104 10-11 -0.01503108501770356 1104 12-13 -0.13446132308340708 1104 14-15 -0.11019159328359507 1104 16-17 -0.04341873183369671 1104 18-19 -0.04838738907156426 1104 20-21 -0.07085467242833232 1104 22-23 -0.0032031201683366817 1104 24-25 -0.15777277904509646 1104 26-27 -0.20416783350290757 1104 28-29 -0.08436349872354754 1104 30-31 -0.22262346797426602 1104 32-33 -0.08517208851075964 1104 34-35 -0.07346955980079173 1104 36-37 -0.1639443699289913 1104 38-39 -0.007603156380284304 1104 40-41 -0.07482035252645147 1104 42-43 -0.056612256330481614 1104 44-45 -0.08658183085677962 1104 46-47 -0.1866397764060892 1104 48-49 -0.20189054310614551 1104 50-51 -0.16167073405892296 1104 52-53 -0.2269229264020538 1104 54-55 -0.18395486736560684 1104 56-57 -0.13352120676486834 1104 58-59 -0.36693137788922314 1104 60-61 -0.2424607459957855 1104 62-63 -0.5538034387049251 1104 64-65 -0.37977038143164066 1104 66-67 -0.40185913692578623 1104 68-69 -0.2862339236540663 1104 70-71 -0.18826961750674087 1104 72-73 -0.37470921032696936 1104 74-75 -0.42722108853567775 1104 76-77 -0.3002388684682451 1104 78-79 -0.13506377110351053 1104 80-81 -0.20858879724383428 1104 82-83 -0.1807848406728958 1104 84-85 -0.23433365309094256 1104 86-87 -0.06891211625517002 1104 88-89 -0.03370942567876867 1104 90-91 0.011267890531197366 1104 92-93 -0.07290157062123725 1104 94-95 0.052754315573270816 1104 96-97 0.10415162919353804 1104 98-99 0.05707150983955245 1104 100-101 0.5564162509524593 1105 1 -0.013860767426258747 1105 2 -0.11901862025490573 1105 3 -0.09564393896786072 1105 4 -0.11371819445199804 1105 5 0.001542277756641397 1105 6 -0.1577193210994352 1105 7 -0.046941123049357714 1105 8 -0.13969771648243068 1105 9 -0.14591846503204664 1105 10-11 -0.11059244272004776 1105 12-13 -0.14177202804425093 1105 14-15 -0.19687566639063903 1105 16-17 -0.10477643418357019 1105 18-19 -0.20948399481568458 1105 20-21 -0.13247347138395327 1105 22-23 -0.17944332904561833 1105 24-25 -0.16403909758295754 1105 26-27 -0.19842370817654142 1105 28-29 -0.13188308096897572 1105 30-31 -0.3620490554416307 1105 32-33 -0.2802112530016245 1105 34-35 -0.2802580715501435 1105 36-37 -0.29658144564701416 1105 38-39 -0.21308618510090582 1105 40-41 -0.2382668277222777 1105 42-43 -0.21692557225737374 1105 44-45 -0.07013274469489517 1105 46-47 -0.013010533586246709 1105 48-49 -0.18961900263617082 1105 50-51 -0.2927412301424752 1105 52-53 -0.3371056940260715 1105 54-55 -0.16776687780946276 1105 56-57 -0.22882146786147928 1105 58-59 -0.20949012462551053 1105 60-61 -0.5043406415571425 1105 62-63 -0.31620552747777 1105 64-65 -0.442955760021718 1105 66-67 -0.40316461995450936 1105 68-69 -0.5050328792676453 1105 70-71 -0.5733871109793327 1105 72-73 -0.5002966776898958 1105 74-75 -0.5170383209116558 1105 76-77 -0.5540247692515337 1105 78-79 -0.7340193846805363 1105 80-81 -0.5963172567738617 1105 82-83 -0.5583305325788999 1105 84-85 -0.6124015382084345 1105 86-87 -0.6104264765684846 1105 88-89 -0.5211767886030536 1105 90-91 -0.4156250598604494 1105 92-93 -0.3112827716656206 1105 94-95 -0.32244150688103446 1105 96-97 -0.5491120783782648 1105 98-99 -0.49880316378969525 1105 100-101 -0.23366207802926553 1106 1 -0.10871677659204693 1106 2 -0.1057445383376816 1106 3 -0.08484285500919242 1106 4 0.024923167368001486 1106 5 -0.016691436459552733 1106 6 -0.0580429846337438 1106 7 -0.14550890188328935 1106 8 -0.040833947947980676 1106 9 -0.06000310953022847 1106 10-11 -0.0885023062359096 1106 12-13 -0.05476937162358553 1106 14-15 -0.05016771624632099 1106 16-17 -0.10781066283895058 1106 18-19 -0.3043151376380706 1106 20-21 -0.1972696810045491 1106 22-23 -0.18890323474068538 1106 24-25 -0.19655050468376345 1106 26-27 -0.2728533531359574 1106 28-29 -0.22001956243486376 1106 30-31 -0.24642403455256812 1106 32-33 -0.22546398729672745 1106 34-35 -0.12517163426880273 1106 36-37 -0.14836868065668085 1106 38-39 -0.03380091867675361 1106 40-41 0.04176937553468463 1106 42-43 -0.09090428078506108 1106 44-45 -0.014271974827558154 1106 46-47 -0.07985126465674597 1106 48-49 0.10979088962304218 1106 50-51 0.036316429025511354 1106 52-53 -0.03542908750655016 1106 54-55 -0.05069319188207544 1106 56-57 0.03455508745477687 1106 58-59 -0.07885746335190191 1106 60-61 0.04813416121665881 1106 62-63 -0.06008352731466715 1106 64-65 0.010894178280338451 1106 66-67 -0.05038045847835804 1106 68-69 0.07424663848571811 1106 70-71 0.05992509933111023 1106 72-73 0.13862302674821336 1106 74-75 0.23233099642699884 1106 76-77 0.008958737410907247 1106 78-79 -0.024661350779418 1106 80-81 -0.039231485268818034 1106 82-83 0.046873618763548563 1106 84-85 -0.06780843046363572 1106 86-87 0.23208440914125816 1106 88-89 0.2903041090986811 1106 90-91 0.27254083340946167 1106 92-93 0.43389589835933506 1106 94-95 0.29198164074349364 1106 96-97 0.45776226236283435 1106 98-99 0.46398488806599403 1106 100-101 0.9504332215633795 1107 1 -0.05890726755025355 1107 2 -0.03536383904503282 1107 3 -0.05757602788352756 1107 4 0.020590366945953065 1107 5 0.011060943372513066 1107 6 -0.0530565849472282 1107 7 -0.12873174460469272 1107 8 -0.021796564463443246 1107 9 -0.0177536586732856 1107 10-11 -0.012806116080540164 1107 12-13 -0.09407444309047008 1107 14-15 0.0017814583268105366 1107 16-17 0.01997321299194965 1107 18-19 -0.03779278731853708 1107 20-21 -0.02628790972762829 1107 22-23 -0.14360908579083542 1107 24-25 -0.09152344282251335 1107 26-27 0.015574757183038912 1107 28-29 -0.08027917399779483 1107 30-31 -0.1340097987684814 1107 32-33 0.13264246408702718 1107 34-35 -0.08524058937313583 1107 36-37 -0.07582043538101857 1107 38-39 0.004805359186192959 1107 40-41 -0.08919949180350528 1107 42-43 -0.11526784900419784 1107 44-45 -0.1944722455862049 1107 46-47 -0.36105464605903137 1107 48-49 -0.45043763332664355 1107 50-51 -0.17025225631315521 1107 52-53 -0.26406343169085034 1107 54-55 -0.21718048157881498 1107 56-57 -0.20132868339796062 1107 58-59 -0.022661675871724185 1107 60-61 -0.12952847920724508 1107 62-63 -0.19089733351064098 1107 64-65 -0.0803786547274683 1107 66-67 -0.24960398153370988 1107 68-69 0.06904597943767499 1107 70-71 -0.12619613913128802 1107 72-73 -0.10574594951469862 1107 74-75 -0.1825522761422249 1107 76-77 -0.18618405505948488 1107 78-79 -0.18329679235866791 1107 80-81 -0.11442951922593636 1107 82-83 -0.03669275186109999 1107 84-85 -0.13254505694935403 1107 86-87 -0.17701182373868107 1107 88-89 0.13372036322378378 1107 90-91 0.19672866566742542 1107 92-93 0.3852554586680661 1107 94-95 0.5416607609331905 1107 96-97 0.5010182102561771 1107 98-99 0.3592172799057529 1107 100-101 0.861167144536438 1108 1 0.008985057210271208 1108 2 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5 57.0 6 123.0 7 381.0 8 832.0 9 1543.0 10 2396.0 11 3670.0 12 5072.0 13 6806.0 14 7723.0 15 9800.0 16 11983.0 17 15610.0 18 15114.0 19 11601.0 20 10975.0 21 11875.0 22 12794.0 23 14275.0 24 16521.0 25 19938.0 26 23583.0 27 29253.0 28 35517.0 29 43711.0 30 52861.0 31 64081.0 32 78904.0 33 99660.0 34 130145.0 35 181768.0 36 273384.0 37 426597.0 38 367112.0 39 40592.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.31635162422395 20.15398075067226 14.915256588346965 23.614411036756824 2 16.289637868766537 21.54645799548138 38.290178760902975 23.87372537484911 3 18.62435732045194 24.70287946100296 31.230302593617274 25.442460624927826 4 13.75845020584499 17.952190267692256 36.36453366313277 31.924825863329982 5 13.938583079749018 36.79769153553158 34.602784804879086 14.660940579840318 6 31.31819756578798 36.22783008485492 17.678980517025764 14.774991832331333 7 27.136005748459496 32.96925107166722 21.847107164748046 18.04763601512524 8 25.356885171955334 34.92796165588008 20.8225212482764 18.892631923888185 9 25.641347058306792 16.84982643087191 21.230707275690154 36.27811923513114 10-11 24.4501999228143 26.447355303950783 28.12212219227136 20.98032258096356 12-13 25.04814236123106 24.188217632096617 28.203181985528175 22.560458021144143 14-15 22.577564666627513 25.900185413938964 26.22852156936095 25.29372835007257 16-17 21.95971114020163 28.603905998418256 27.19602910313055 22.24035375824957 18-19 21.863612136526047 27.31357462937008 29.145182304518624 21.677630929585252 20-21 22.651264730180696 26.650288558058556 29.056616996054846 21.64182971570591 22-23 22.4548211946807 26.547341386835594 28.854152580933945 22.14368483754976 24-25 22.3470869306444 26.776801057305942 28.940961820713994 21.93515019133566 26-27 22.110394417355945 26.8958140039876 28.931983733738676 22.06180784491778 28-29 22.00742542252263 27.0133426640517 29.02286058899995 21.95637132442572 30-31 22.419140080206013 26.75898517580475 28.79270999791737 22.02916474607187 32-33 22.190642763445563 26.81226009015527 28.675574278407446 22.32152286799172 34-35 22.526110783309928 26.935682446283586 28.56406053620048 21.974146234206007 36-37 22.649979342810138 26.649922085127365 28.567264696837846 22.13283387522465 38-39 22.23659330749993 26.820048182209895 28.636826469515448 22.306532040774727 40-41 22.449419757893963 26.61767195406826 28.76500947369304 22.167898814344735 42-43 22.34913372963876 26.67804255718174 28.462601451474796 22.510222261704705 44-45 22.278064515492225 26.878403468095126 28.4703900126441 22.373142003768553 46-47 22.530385526976524 26.79182382254926 28.056868529465106 22.620922121009105 48-49 22.295901283163904 26.841397328750883 28.15486306066707 22.707838327418145 50-51 22.21421080554219 27.05279391987761 28.055569949784704 22.6774253247955 52-53 22.369689904958932 27.09583365000528 27.88303898470203 22.651437460333753 54-55 22.210728812672667 27.038684645079016 27.812984569768666 22.937601972479644 56-57 22.144844597148865 27.21407977764201 27.654245559107764 22.986830066101362 58-59 22.07684851786927 27.003926422513857 27.878044013834273 23.0411810457826 60-61 22.100580969545984 27.1278527815669 27.870153405176413 22.901412843710702 62-63 21.919505774615775 27.03110919408059 28.00298773813319 23.046397293170447 64-65 21.873386823749115 27.072292997717 27.76908643515562 23.285233743378267 66-67 21.774202503377523 27.244224279924246 27.51886786633025 23.46270535036798 68-69 21.92174385533214 26.941635093859702 27.611067434777908 23.52555361603025 70-71 21.925244534660514 26.781465137632566 27.507487366418648 23.785802961288276 72-73 21.839827822583434 26.893541965037443 27.39014115508108 23.87648905729805 74-75 21.76259276843006 27.200902934537247 27.119324950821255 23.917179346211437 76-77 21.8471935877711 26.875352433249788 27.36981489335058 23.907639085628528 78-79 21.82502930051018 26.99218784712588 27.23441691737898 23.94836593498496 80-81 21.856637392188595 26.912178786609857 27.101737838498746 24.129445982702798 82-83 21.8830597123204 26.86699087293871 27.14168116777428 24.10826824696661 84-85 21.80491165592943 26.81206268426606 27.021288251092397 24.361737408712113 86-87 21.767310778106157 26.68687860701706 27.109347371636343 24.43646324324044 88-89 21.776437761748525 26.549671351413977 26.949901978526132 24.723988908311366 90-91 21.602903509099697 26.580757744212764 27.020208706419584 24.796130040267954 92-93 21.629047684379568 26.46929203338923 26.986594166063753 24.915066116167445 94-95 21.601243460002966 26.27746934470852 27.03523671316152 25.086050482126993 96-97 21.676104313219977 26.088693478992557 27.014305017761597 25.22089719002587 98-99 21.46020930987752 26.22024223724484 26.936629623378433 25.38291882949921 100-101 21.541823544130253 25.867027133200377 27.159011441259107 25.43213788141026 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 905.0 1 629.0 2 397.5 3 451.0 4 444.0 5 453.0 6 682.0 7 1219.0 8 1758.0 9 2009.0 10 1804.5 11 1576.0 12 1634.0 13 1732.0 14 1932.0 15 2118.5 16 2237.0 17 2382.0 18 2582.5 19 2884.5 20 3277.0 21 3880.0 22 4661.5 23 5708.5 24 6900.0 25 8523.0 26 11274.0 27 14633.0 28 17150.5 29 19924.0 30 23093.0 31 26632.5 32 31106.0 33 34718.5 34 38067.0 35 41857.0 36 46489.0 37 50409.5 38 53613.5 39 55699.0 40 57247.0 41 60074.5 42 61770.0 43 64196.0 44 67898.5 45 71136.5 46 72696.5 47 73623.5 48 75663.0 49 76390.0 50 75598.0 51 75319.0 52 75405.5 53 74775.0 54 72472.5 55 68045.0 56 62827.0 57 56642.5 58 51036.5 59 45762.5 60 38366.5 61 30538.5 62 24153.0 63 18407.5 64 13233.5 65 9515.5 66 6904.0 67 5400.5 68 4166.0 69 3092.0 70 2270.5 71 1492.5 72 1041.5 73 755.5 74 492.0 75 341.5 76 220.5 77 110.5 78 69.5 79 43.0 80 26.0 81 21.5 82 13.0 83 10.0 84 7.0 85 3.5 86 4.0 87 1.5 88 1.0 89 3.0 90 2.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38089466323048815 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.46757361512366E-4 16-17 0.0019493831559476915 18-19 9.87029446049464E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.87029446049464E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007674153943034582 36-37 0.01742106972277304 38-39 0.011005378323451524 40-41 0.007155963483858614 42-43 0.0033065486442657045 44-45 0.001974058892098928 46-47 0.0014558684329229593 48-49 7.40272084537098E-5 50-51 3.454603061173124E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.93514723024732E-4 60-61 6.909206122346248E-4 62-63 0.0 64-65 0.0 66-67 9.623537098982274E-4 68-69 6.415691399321516E-4 70-71 0.0017519772667377986 72-73 0.0 74-75 0.0 76-77 0.09685226439360366 78-79 0.0355330600577807 80-81 2.46757361512366E-5 82-83 0.0 84-85 0.0 86-87 0.15079342362020687 88-89 0.3243132002357026 90-91 0.28093325608182873 92-93 0.0 94-95 2.46757361512366E-5 96-97 0.0 98-99 1.974058892098928E-4 100-101 9.376779737469909E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2026282.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.162007250081544 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26909625411848 42.834255367809696 2 13.458541589247734 15.117174206217104 3 4.320841994544062 7.280014782721213 4 1.875920526457781 4.214218488299947 5 1.0268694818457291 2.8835525642153663 6 0.6382076328814212 2.150581302296626 7 0.426764024995217 1.6777546986098753 8 0.30161972854368224 1.355165550499033 9 0.24356787153860357 1.2311334510514185 >10 1.3221589302612047 13.569564087157952 >50 0.08021106314513907 3.082200734350984 >100 0.03470798808100492 3.356474261148258 >500 8.781849058656127E-4 0.3106213091617608 >1k 4.3909245293280295E-4 0.41897872679090825 >5k 1.756369811731212E-4 0.5183104696698828 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5418 0.2673862769347998 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5091 0.25124834549189107 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3236 0.1597013643708033 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.87029446049464E-5 2 4.93514723024732E-5 0.0 0.0 0.0 9.87029446049464E-5 3 4.93514723024732E-5 0.0 0.0 0.0 9.87029446049464E-5 4 4.93514723024732E-5 0.0 0.0 0.0 9.87029446049464E-5 5 4.93514723024732E-5 0.0 0.0 0.0 9.87029446049464E-5 6 4.93514723024732E-5 0.0 0.0 0.0 1.974058892098928E-4 7 4.93514723024732E-5 0.0 0.0 0.0 1.974058892098928E-4 8 4.93514723024732E-5 0.0 0.0 0.0 1.974058892098928E-4 9 4.93514723024732E-5 0.0 0.0 0.0 2.46757361512366E-4 10-11 4.93514723024732E-5 0.0 0.0 4.93514723024732E-5 2.46757361512366E-4 12-13 4.93514723024732E-5 0.0 0.0 4.93514723024732E-5 7.649478206883345E-4 14-15 4.93514723024732E-5 0.0 0.0 4.93514723024732E-5 0.0010117051822007006 16-17 4.93514723024732E-5 0.0 0.0 4.93514723024732E-5 0.00123378680756183 18-19 4.93514723024732E-5 0.0 0.0 9.87029446049464E-5 0.00123378680756183 20-21 4.93514723024732E-5 0.0 0.0 1.4805441690741961E-4 0.00123378680756183 22-23 4.93514723024732E-5 0.0 0.0 4.1948751457102215E-4 0.0013078140160155398 24-25 4.93514723024732E-5 0.0 0.0 0.001357165488318013 0.0013818412244692496 26-27 4.93514723024732E-5 0.0 0.0 0.0034546030611731244 0.0014311926967717228 28-29 4.93514723024732E-5 0.0 0.0 0.01226384086716459 0.0014311926967717228 30-31 4.93514723024732E-5 0.0 0.0 0.03792660646445065 0.0014558684329229593 32-33 4.93514723024732E-5 0.0 0.0 0.09542107169683194 0.0017026257944353254 34-35 4.93514723024732E-5 0.0 0.0 0.18992914115606813 0.0018260044751915083 36-37 4.93514723024732E-5 0.0 0.0 0.3354419572399103 0.0018260044751915083 38-39 4.93514723024732E-5 0.0 0.0 0.5204112754295799 0.0019493831559476915 40-41 4.93514723024732E-5 0.0 0.0 0.7161638903173398 0.001974058892098928 42-43 4.93514723024732E-5 0.0 0.0 0.9253894571436749 0.001974058892098928 44-45 4.93514723024732E-5 0.0 0.0 1.1945523870813637 0.001998734628250165 46-47 4.93514723024732E-5 0.0 0.0 1.5049978236000714 0.002146789045157584 48-49 4.93514723024732E-5 0.0 0.0 1.8124574960444795 0.002270167725913767 50-51 4.93514723024732E-5 0.0 0.0 2.1250990730806474 0.0023688706705187134 52-53 4.93514723024732E-5 0.0 0.0 2.4446745319753127 0.0025662765597286065 54-55 4.93514723024732E-5 0.0 0.0 2.815427467647642 0.0025662765597286065 56-57 4.93514723024732E-5 0.0 0.0 3.257986795520071 0.0026156280320310797 58-59 4.93514723024732E-5 0.0 0.0 3.7111813656736823 0.002664979504333553 60-61 4.93514723024732E-5 0.0 0.0 4.175726774456862 0.002689655240484789 62-63 4.93514723024732E-5 0.0 0.0 4.652289266745695 0.0028623853935434455 64-65 4.93514723024732E-5 0.0 0.0 5.173046002481392 0.0029364126019971555 66-67 4.93514723024732E-5 0.0 0.0 5.767607865045438 0.002985764074299628 68-69 4.93514723024732E-5 0.0 0.0 6.403945748913527 0.003010439810450865 70-71 4.93514723024732E-5 0.0 0.0 7.079888189304352 0.003010439810450865 72-73 4.93514723024732E-5 0.0 0.0 7.762468402719858 0.003035115546602102 74-75 4.93514723024732E-5 0.0 0.0 8.481001163707717 0.0031091427550558118 76-77 4.93514723024732E-5 0.0 0.0 9.264998652704806 0.003281872908114468 78-79 4.93514723024732E-5 0.0 0.0 10.111104969594557 0.0033805758527194144 80-81 4.93514723024732E-5 0.0 0.0 10.986501385295828 0.003405251588870651 82-83 4.93514723024732E-5 0.0 0.0 11.874877238212648 0.0036520089503830166 84-85 4.93514723024732E-5 0.0 0.0 12.796614686405938 0.0036520089503830166 86-87 9.87029446049464E-5 0.0 0.0 13.751318918097283 0.0037753876311391998 88-89 9.87029446049464E-5 0.0 0.0 14.769809927739574 0.003898766311895383 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4250 0.0 45.95652 1 GTATCAA 6865 0.0 33.23894 1 ATCAACG 8505 0.0 26.50046 3 TCAACGC 8540 0.0 26.39185 4 CAACGCA 8670 0.0 26.050854 5 TATCAAC 8685 0.0 26.005861 2 AACGCAG 9050 0.0 24.904577 6 ACGCAGA 10060 0.0 22.262716 7 CGCAGAG 10145 0.0 21.795557 8 GAGTACT 6265 0.0 20.677967 12-13 GCAGAGT 11015 0.0 19.901764 9 AGAGTAC 9230 0.0 19.87074 10-11 GTGGTAT 2460 0.0 19.364983 1 CAGAGTA 10545 0.0 18.60781 10-11 GTACTTT 6790 0.0 18.589947 14-15 GTACATG 6030 0.0 18.565332 1 TACATGG 5850 0.0 18.493244 2 TGGTATC 2445 0.0 18.436516 2 ACATGGG 5815 0.0 18.11496 3 AGTACTT 6860 0.0 17.777689 12-13 >>END_MODULE