FastQCFastQC Report
Thu 26 May 2016
SRR938762_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938762_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022488
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58180.2876654892389967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50260.24850580077607382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.14941992239261742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39500.048.35861
GTATCAA65950.036.384911
TCAACGC81350.028.9815624
ATCAACG81800.028.6481533
CAACGCA82800.028.4167445
TATCAAC83000.028.348272
AACGCAG85850.027.4617566
ACGCAGA98350.023.6832267
CGCAGAG98600.023.478848
GTACATG59600.022.8015581
TACATGG58800.022.0246122
GAGTACT62150.021.19510312-13
GCAGAGT107400.020.6711449
ACATGGG58800.020.6531163
CAGAGTA99400.019.7928710-11
GTACTTT67050.019.68205814-15
AGAGTAC89100.019.5505510-11
GTGGTAT23600.018.7247371
AGTACTT67650.018.52464312-13
TAGGACA7850.018.129034
Produced by FastQC (version 0.11.5)