##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938762_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2022488 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.405803149388277 33.0 31.0 34.0 30.0 34.0 2 31.480017186752157 33.0 31.0 34.0 28.0 34.0 3 31.492310955615064 33.0 31.0 34.0 28.0 34.0 4 35.051777810300976 37.0 35.0 37.0 32.0 37.0 5 35.07948427876951 37.0 35.0 37.0 32.0 37.0 6 35.12950484749477 37.0 35.0 37.0 32.0 37.0 7 35.0302370150033 37.0 35.0 37.0 32.0 37.0 8 35.00157133194362 37.0 35.0 37.0 32.0 37.0 9 36.551148881971116 39.0 37.0 39.0 32.0 39.0 10-11 36.43054000814838 39.0 37.0 39.0 32.0 39.0 12-13 36.32147187029045 39.0 37.0 39.0 32.0 39.0 14-15 37.46178765955595 40.0 37.0 41.0 32.0 41.0 16-17 37.33572708466008 40.0 37.0 41.0 32.0 41.0 18-19 37.263536297866786 40.0 37.0 41.0 31.0 41.0 20-21 37.145609022154886 40.0 37.0 41.0 31.0 41.0 22-23 37.13624407165827 40.0 37.0 41.0 31.0 41.0 24-25 37.06636207483061 40.0 37.0 41.0 31.0 41.0 26-27 36.92691748974531 40.0 36.0 41.0 30.0 41.0 28-29 36.75148974925933 39.5 36.0 41.0 30.0 41.0 30-31 36.5263521959092 39.0 36.0 41.0 30.0 41.0 32-33 36.338424752087526 39.0 35.5 41.0 30.0 41.0 34-35 36.042924605733134 39.0 35.0 41.0 28.5 41.0 36-37 35.83277082484544 39.0 35.0 41.0 27.5 41.0 38-39 35.66020663657831 39.0 35.0 40.0 27.0 41.0 40-41 35.552266564746 38.5 35.0 40.5 27.0 41.0 42-43 35.56784885744687 39.0 35.0 41.0 26.5 41.0 44-45 35.40572725276985 39.0 35.0 41.0 26.0 41.0 46-47 35.1209359462207 39.0 35.0 41.0 24.5 41.0 48-49 34.845847540257346 39.0 34.0 40.5 24.0 41.0 50-51 33.704660299591396 37.5 32.5 39.5 23.0 40.5 52-53 33.77152472598107 37.5 33.0 39.5 23.0 40.5 54-55 34.27127503352307 38.0 34.0 40.0 23.0 41.0 56-57 34.16448132201526 38.0 34.0 40.0 22.5 41.0 58-59 33.950108974688604 38.0 33.5 40.0 22.0 41.0 60-61 33.644565999897154 37.5 33.0 40.0 21.0 41.0 62-63 33.22799418340183 37.0 32.5 40.0 19.5 41.0 64-65 32.81699199204148 36.0 32.0 39.5 18.0 41.0 66-67 32.42192364058526 36.0 32.0 39.0 15.5 41.0 68-69 31.975326182405038 35.0 31.0 39.0 12.0 40.0 70-71 31.5441300516987 35.0 31.0 38.0 8.0 40.0 72-73 31.104791227438678 35.0 31.0 37.5 7.0 40.0 74-75 30.619196998943877 35.0 30.0 37.0 3.5 39.0 76-77 30.1446156911685 34.5 30.0 36.0 2.0 39.0 78-79 29.68598750647717 34.0 29.0 36.0 2.0 38.5 80-81 29.2029495354237 34.0 29.0 35.0 2.0 37.0 82-83 28.774918318427602 34.0 29.0 35.0 2.0 37.0 84-85 28.40107926474718 34.0 28.5 35.0 2.0 36.5 86-87 27.944378656387578 34.0 27.0 35.0 2.0 36.0 88-89 27.457398758360988 34.0 26.0 35.0 2.0 36.0 90-91 26.965456160926543 33.0 25.5 35.0 2.0 35.0 92-93 26.454064498775764 33.0 25.0 35.0 2.0 35.0 94-95 25.896724479947473 33.0 23.5 35.0 2.0 35.0 96-97 25.242442476790963 32.5 19.0 35.0 2.0 35.0 98-99 24.416573794257367 32.0 4.5 35.0 2.0 35.0 100-101 21.99755449723311 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13105498796770476 1101 2 0.3132896833559613 1101 3 0.3820315057110477 1101 4 -3.975830043145834E-4 1101 5 0.06059507445782231 1101 6 0.1812769051202352 1101 7 0.2808784457369313 1101 8 0.25156762600203564 1101 9 0.2539316552929378 1101 10-11 0.19617226675534738 1101 12-13 0.18983179766571112 1101 14-15 0.19154951197555903 1101 16-17 0.4363365784608746 1101 18-19 0.47164298832044693 1101 20-21 0.5633446720739741 1101 22-23 0.33671739101728804 1101 24-25 0.34771943533417016 1101 26-27 0.47551819208624835 1101 28-29 0.45901137562014327 1101 30-31 0.44963358148289245 1101 32-33 0.3287835330657245 1101 34-35 0.37628064930200367 1101 36-37 0.2824526503873699 1101 38-39 0.2829457075167241 1101 40-41 0.3609329800131107 1101 42-43 0.45328308618856994 1101 44-45 0.42663718325357536 1101 46-47 0.28983434663196306 1101 48-49 0.29644061387895704 1101 50-51 0.1573400091991104 1101 52-53 0.3315016721503312 1101 54-55 0.2900456871373507 1101 56-57 0.25082096386089603 1101 58-59 0.08032700783437718 1101 60-61 0.0182587905662146 1101 62-63 -7.151973738572792E-4 1101 64-65 -0.05916971763991086 1101 66-67 -0.1868187207671781 1101 68-69 -0.02652401275613414 1101 70-71 -0.21997650941444036 1101 72-73 -0.20762993305148214 1101 74-75 -0.25019825126637585 1101 76-77 -0.37483301910926414 1101 78-79 -0.5380175651091825 1101 80-81 -0.5894700331705742 1101 82-83 -0.5350756089103825 1101 84-85 -0.6524211097116535 1101 86-87 -0.7633569654564418 1101 88-89 -0.6822334604506146 1101 90-91 -0.6016576131543339 1101 92-93 -0.6850645393459764 1101 94-95 -0.6740784263642006 1101 96-97 -0.5809578308792247 1101 98-99 -0.61200460947747 1101 100-101 -0.5543642561793476 1102 1 0.12576291738059098 1102 2 0.07535177376870905 1102 3 0.23293880193744698 1102 4 0.39862347367120066 1102 5 0.37408576033507757 1102 6 0.38346585716575277 1102 7 0.2815794936633722 1102 8 0.30419220922904344 1102 9 0.510010441863102 1102 10-11 0.37383197248346534 1102 12-13 0.3231015693203787 1102 14-15 0.44594648457019304 1102 16-17 0.5376646436527253 1102 18-19 0.5495341800738771 1102 20-21 0.5718739015662138 1102 22-23 0.6008983286829164 1102 24-25 0.4585587579536181 1102 26-27 0.5241062815623962 1102 28-29 0.571742546173887 1102 30-31 0.668987692007299 1102 32-33 0.6229534782998556 1102 34-35 0.5890531816834041 1102 36-37 0.5277399258066637 1102 38-39 0.4967075587319201 1102 40-41 0.43744871573486677 1102 42-43 0.6277800843013566 1102 44-45 0.6073460268329569 1102 46-47 0.48817833122012644 1102 48-49 0.400565299458961 1102 50-51 0.5707950818416947 1102 52-53 0.5321955693879161 1102 54-55 0.3744684022118463 1102 56-57 0.4797947138223364 1102 58-59 0.2019258738192562 1102 60-61 0.19231090366487535 1102 62-63 -0.0015286933876907938 1102 64-65 -0.027979814973406292 1102 66-67 0.06795821980358596 1102 68-69 0.09960824974986338 1102 70-71 0.0738032325438418 1102 72-73 -0.20599060258028956 1102 74-75 -0.3614966200680598 1102 76-77 -0.1820578788295748 1102 78-79 -0.39144456779279935 1102 80-81 -0.4647479986734062 1102 82-83 -0.37164325022061107 1102 84-85 -0.4598203901560609 1102 86-87 -0.6278993634329382 1102 88-89 -0.6972727611973717 1102 90-91 -0.7005579493769183 1102 92-93 -0.7296537112401253 1102 94-95 -0.9306388328598345 1102 96-97 -1.2327724233320225 1102 98-99 -1.375014797105937 1102 100-101 -0.9063647665422394 1103 1 0.33589777759204154 1103 2 0.42470102646732855 1103 3 0.5110610617054512 1103 4 0.5157811065024163 1103 5 0.4694762957505674 1103 6 0.41443143060451604 1103 7 0.5771179336105376 1103 8 0.48616999580698916 1103 9 0.5990428231097411 1103 10-11 0.5477160793011038 1103 12-13 0.5782182556061102 1103 14-15 0.5775303848980968 1103 16-17 0.6885513364878122 1103 18-19 0.7093593328741079 1103 20-21 0.703510702650668 1103 22-23 0.6495327548089733 1103 24-25 0.7332820421196189 1103 26-27 0.6395816975336928 1103 28-29 0.5649711133439794 1103 30-31 0.6676564696272109 1103 32-33 0.6556235712311249 1103 34-35 0.6757636412917094 1103 36-37 0.8508857610892164 1103 38-39 0.8321893336733339 1103 40-41 0.9803060800641461 1103 42-43 1.016489811730544 1103 44-45 0.9139785305808843 1103 46-47 0.8863919719453861 1103 48-49 0.846524932781918 1103 50-51 1.0105923889892026 1103 52-53 1.0023403678700333 1103 54-55 1.0683926483284694 1103 56-57 1.0184680226844236 1103 58-59 1.0327852921666008 1103 60-61 0.8114797171865789 1103 62-63 0.9408013224743783 1103 64-65 0.851734879959217 1103 66-67 0.8370942865330662 1103 68-69 0.7768149048554207 1103 70-71 0.7616078538669022 1103 72-73 0.8257728339398902 1103 74-75 0.6212584132553403 1103 76-77 0.5127354899993968 1103 78-79 0.41043557133211195 1103 80-81 0.5002533231655484 1103 82-83 0.5531771591904437 1103 84-85 0.6398828488389512 1103 86-87 0.5067370057419929 1103 88-89 0.5919067086309759 1103 90-91 0.5937140002213077 1103 92-93 0.46653030832526454 1103 94-95 0.2525558071104008 1103 96-97 -0.014639349507046262 1103 98-99 0.16686573911463043 1103 100-101 0.6355066582369631 1104 1 0.13843740946777316 1104 2 0.1340685123700851 1104 3 0.060471105327117414 1104 4 0.29363779338400064 1104 5 0.27990400082557443 1104 6 0.188524951191809 1104 7 0.20273502053468206 1104 8 0.3448927322683204 1104 9 0.38293206664628343 1104 10-11 0.4000636694702777 1104 12-13 0.4197851776190973 1104 14-15 0.37718779526090884 1104 16-17 0.46991398145004126 1104 18-19 0.4325657910079528 1104 20-21 0.4887046062574356 1104 22-23 0.28666117272577907 1104 24-25 0.40278730863733614 1104 26-27 0.3991093068538376 1104 28-29 0.5122583247494461 1104 30-31 0.46160396404150816 1104 32-33 0.22780039855317824 1104 34-35 0.48297611070334767 1104 36-37 0.4642132427362 1104 38-39 0.5351149108624043 1104 40-41 0.49293215730641293 1104 42-43 0.4167933014634926 1104 44-45 0.48722541854768764 1104 46-47 0.5624519251318958 1104 48-49 0.601489290692335 1104 50-51 0.5954093907979043 1104 52-53 0.5370645406543773 1104 54-55 0.5050079797467006 1104 56-57 0.4712885355049323 1104 58-59 0.6694000916133476 1104 60-61 0.5775019003975572 1104 62-63 0.7724988434872486 1104 64-65 0.8791231659470924 1104 66-67 0.6209119799046974 1104 68-69 0.5015042335074114 1104 70-71 0.46536011474571737 1104 72-73 0.3691697378658354 1104 74-75 0.34272372871443224 1104 76-77 0.40801903080160784 1104 78-79 0.2189356777264102 1104 80-81 -0.012282318924032865 1104 82-83 -0.07461489315554815 1104 84-85 0.08736635772287116 1104 86-87 0.12662795158729878 1104 88-89 0.32186361894058635 1104 90-91 0.10197737930418782 1104 92-93 0.1258637480528968 1104 94-95 0.20522364411433713 1104 96-97 0.19293220065786798 1104 98-99 0.3239367041109027 1104 100-101 0.4494791553111206 1105 1 0.12092094808424392 1105 2 0.2571981118743061 1105 3 0.20657227505366293 1105 4 0.42628168524239385 1105 5 0.2950593188207691 1105 6 0.35753262025990296 1105 7 0.4300725369960645 1105 8 0.35510576193753707 1105 9 0.3706228250319086 1105 10-11 0.3515362854746158 1105 12-13 0.41270097280074935 1105 14-15 0.3891035129772291 1105 16-17 0.33514831408619017 1105 18-19 0.30227452135113 1105 20-21 0.43418063073648483 1105 22-23 0.30132768446177494 1105 24-25 0.29068489946543963 1105 26-27 0.5470835883628595 1105 28-29 0.5547217223790071 1105 30-31 0.491273187064202 1105 32-33 0.5751557320414449 1105 34-35 0.49929495801917056 1105 36-37 0.5371747424650692 1105 38-39 0.5666231609204999 1105 40-41 0.5886785358020319 1105 42-43 0.5455414616618057 1105 44-45 0.7547782886383274 1105 46-47 0.7623047409967896 1105 48-49 0.6487191303390958 1105 50-51 0.661187530080575 1105 52-53 0.6881250410804327 1105 54-55 0.5694041374661225 1105 56-57 0.46509440737727203 1105 58-59 0.6458754189043887 1105 60-61 0.5905950911946789 1105 62-63 0.5345253366315674 1105 64-65 0.4302301455737094 1105 66-67 0.40694188152372845 1105 68-69 0.34440995819560527 1105 70-71 0.26315002875919546 1105 72-73 0.40707352592068347 1105 74-75 0.4244426844817255 1105 76-77 0.34015397541389447 1105 78-79 0.4054543856046351 1105 80-81 0.29344701719805144 1105 82-83 0.17177367296111257 1105 84-85 0.4513674422375509 1105 86-87 0.4158889322984294 1105 88-89 0.4537607449766803 1105 90-91 0.5284294669949468 1105 92-93 0.49119413770352693 1105 94-95 0.5641399040879982 1105 96-97 0.36552961331570444 1105 98-99 0.45638615798029747 1105 100-101 0.7339752433117397 1106 1 0.06297584569426462 1106 2 0.07210582221390993 1106 3 0.07330326165384449 1106 4 0.030238902048289162 1106 5 0.08723485358584782 1106 6 0.08582315151074482 1106 7 0.14149524024258398 1106 8 0.09318594612915376 1106 9 -0.02863297246070573 1106 10-11 0.15833764623388902 1106 12-13 0.24366828694149234 1106 14-15 0.21738345678923565 1106 16-17 0.40784599349854034 1106 18-19 0.27064790303873565 1106 20-21 0.3022174014582859 1106 22-23 0.22092826463354243 1106 24-25 0.22687569803668595 1106 26-27 0.16795659973863764 1106 28-29 0.20222288799140387 1106 30-31 -0.10398567830035432 1106 32-33 0.04279470335991675 1106 34-35 0.10139639065373984 1106 36-37 0.30545099807070386 1106 38-39 0.27011174686614936 1106 40-41 0.33761208744537186 1106 42-43 0.3462483347487364 1106 44-45 0.44811386869576353 1106 46-47 0.5554455718274127 1106 48-49 0.5158619335151258 1106 50-51 0.47805018863120097 1106 52-53 0.2297878555099615 1106 54-55 0.2951760512277062 1106 56-57 0.5702942813221341 1106 58-59 0.5259219738114425 1106 60-61 0.5446269668414843 1106 62-63 0.4823160910636304 1106 64-65 0.5447462200893938 1106 66-67 0.5842799906369791 1106 68-69 0.46422248596899607 1106 70-71 0.3420632306492273 1106 72-73 0.23298247991155208 1106 74-75 0.09453809072622477 1106 76-77 0.14577158098258636 1106 78-79 0.3463677783668011 1106 80-81 0.33771717483656616 1106 82-83 0.2078917512365841 1106 84-85 0.4004746676426336 1106 86-87 0.4359113697157291 1106 88-89 0.539177765800634 1106 90-91 0.765147450430824 1106 92-93 0.7797453386570936 1106 94-95 0.8753643786677081 1106 96-97 0.8784532475609446 1106 98-99 1.0737638233555948 1106 100-101 1.7482712681012593 1107 1 -0.028425365438479844 1107 2 -0.06318090487933503 1107 3 0.05511334714327276 1107 4 0.042702858753415285 1107 5 0.039995213960267506 1107 6 -0.10020414579815906 1107 7 -0.07026082420029667 1107 8 0.03353112286323068 1107 9 0.04011510100323079 1107 10-11 0.11128212492786105 1107 12-13 0.20598792414792655 1107 14-15 0.12127515717435244 1107 16-17 0.19124822691473042 1107 18-19 0.09411160027115528 1107 20-21 0.03344464882356846 1107 22-23 0.09362276186128327 1107 24-25 -0.02993692365210876 1107 26-27 0.012236397154836709 1107 28-29 0.016713072899854353 1107 30-31 -0.040999410844470674 1107 32-33 -0.003950133523446198 1107 34-35 0.16633528775818007 1107 36-37 0.09740266201257697 1107 38-39 -0.12786573168345683 1107 40-41 -0.18539636200036824 1107 42-43 -0.12113565363937084 1107 44-45 0.009330161630693112 1107 46-47 0.07989770399071006 1107 48-49 0.06915626140278164 1107 50-51 -0.0199559684804953 1107 52-53 -0.006050641265154866 1107 54-55 -0.1379855846599085 1107 56-57 -0.07071181921281067 1107 58-59 0.09859310874114868 1107 60-61 0.009295758713157909 1107 62-63 -0.060910500791663935 1107 64-65 -0.015133447005176492 1107 66-67 -0.24522083655420346 1107 68-69 -0.04247088557640666 1107 70-71 -0.0270998528229498 1107 72-73 -0.06854309278177695 1107 74-75 -0.26368348725800317 1107 76-77 -0.3181107452194709 1107 78-79 -0.2533889903591273 1107 80-81 -0.4107615932728734 1107 82-83 -0.5557132513191227 1107 84-85 -0.2845037649081199 1107 86-87 -0.37644694453107874 1107 88-89 -0.17105292104117353 1107 90-91 -0.12123154944821835 1107 92-93 0.02454369129806011 1107 94-95 0.01471487751861389 1107 96-97 0.11941998823261102 1107 98-99 0.3630273598319924 1107 100-101 0.6652329305214195 1108 1 0.11863391078577123 1108 2 0.22526429478081766 1108 3 -0.03979089643021183 1108 4 0.09376559911927984 1108 5 0.11129947969769205 1108 6 0.15593608626650024 1108 7 0.14995125186306524 1108 8 0.1964735294565898 1108 9 0.23528449096132675 1108 10-11 0.2702178351384603 1108 12-13 0.37489740703038166 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25 26997.0 26 31122.0 27 35796.0 28 41991.0 29 48622.0 30 56974.0 31 67794.0 32 82401.0 33 102490.0 34 132449.0 35 180226.0 36 258341.0 37 358353.0 38 296144.0 39 45509.0 40 9.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.56592578196462 19.433982002009913 14.372313774446543 23.62777844157892 2 16.015574508084153 21.000823594726022 38.98837012931378 23.995231767876042 3 18.66301529474359 24.44746413607041 31.003288414457135 25.886232154728866 4 13.496667581195828 17.276182194018187 36.852768398626424 32.374381826159556 5 14.042704188943537 36.454325231392 34.6219226202158 14.881047959448662 6 31.35577981006015 35.68785701653441 18.082728016252535 14.873635157152915 7 27.11683466273084 32.66313328106275 22.161652886991465 18.05837916921494 8 25.286561000343983 34.35673833459064 21.191883605132176 19.164817059933206 9 25.873084645722898 16.739942610551438 21.481281582146387 35.90569116157927 10-11 24.430633205583916 26.17483264628076 28.337828220260114 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22.57850505331295 26.543494231876576 28.641041042034825 22.23695967277565 38-39 22.22737280110538 26.743680504408214 28.702436278957116 22.326510415529295 40-41 22.45347608440041 26.583078243526835 28.73305120483422 22.230394467238533 42-43 22.245346285676284 26.693884519248734 28.46415330402116 22.596615891053816 44-45 22.22132607837332 26.796978666178944 28.49788726504866 22.483807990399075 46-47 22.506607699924803 26.79601627045285 28.121899344565804 22.575476685056547 48-49 22.265347297517344 26.927522072227717 28.111930917799572 22.69519971245537 50-51 22.27066908881695 27.013147669342235 28.06908638453861 22.647096857302206 52-53 22.396581311986306 26.992038052934365 27.908538132112028 22.7028425029673 54-55 22.282006155657573 26.905276784940902 27.9052422862552 22.907474773146323 56-57 22.123785546508415 27.172740857200566 27.82279016238702 22.880683433904004 58-59 22.180360757237434 26.774283736055953 28.059342781890738 22.986012724815872 60-61 22.116687531123087 26.822821086128872 28.158138030778872 22.90235335196916 62-63 22.04132632360003 26.63772277141726 28.33586664797184 22.98508425701087 64-65 21.987378304804356 26.72535475244905 28.087343004571157 23.19992393817544 66-67 21.893420759948203 26.860174019082123 27.8759721791908 23.370433041778877 68-69 21.974841405719324 26.58718415209162 28.064633376101817 23.37334106608724 70-71 21.99366915762556 26.385064177362892 27.991385089004194 23.62988157600735 72-73 21.89148369107577 26.446990038289826 27.950903425430162 23.710622845204238 74-75 21.743119471020123 26.74841557498908 27.79572106573449 23.712743888256302 76-77 21.836197679070835 26.45425402747429 27.94655648094005 23.76299181251483 78-79 21.796781465449325 26.48617464352577 27.955941485250346 23.761102405774555 80-81 21.806676622886105 26.323281323750347 27.887489757154604 23.982552296208944 82-83 21.899848583332155 26.189350501665437 28.007541795939172 23.903259119063236 84-85 21.766283184478972 26.132589499997273 27.964359337221268 24.13676797830249 86-87 21.691612690917058 25.97328209700968 28.1285729282507 24.20653228382256 88-89 21.882938391566302 25.81093675347319 28.011168816663872 24.29495603829663 90-91 21.711591480843218 25.84027052696854 28.095919703814477 24.352218288373763 92-93 21.76537832815954 25.658619050397004 28.05835690605129 24.517645715392167 94-95 21.665220938252087 25.38184360412894 28.227330076430786 24.725605381188185 96-97 21.739954031024343 25.099269253170487 28.262230711823978 24.8985460039812 98-99 21.626107087220163 25.18715747792032 28.158539234488366 25.02819620037115 100-101 21.97448095329219 24.618913203025315 28.446770209197215 24.959835634485287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2202.0 1 1492.5 2 759.5 3 748.0 4 736.0 5 755.0 6 983.0 7 1477.5 8 2093.0 9 2412.5 10 2210.5 11 1972.0 12 2013.0 13 2173.5 14 2334.5 15 2470.5 16 2625.5 17 2807.0 18 3004.5 19 3322.0 20 3768.0 21 4256.5 22 5042.5 23 6104.5 24 7228.0 25 8862.0 26 11497.5 27 14856.0 28 17514.5 29 20139.0 30 23178.5 31 26734.0 32 31193.5 33 34673.5 34 37901.5 35 42219.0 36 46994.5 37 50941.0 38 53700.0 39 55691.5 40 57486.0 41 59483.0 42 61168.5 43 64175.0 44 67549.0 45 70236.5 46 72068.5 47 73051.0 48 74607.5 49 74921.5 50 73546.0 51 72929.5 52 73046.5 53 72625.0 54 70597.5 55 66082.0 56 60719.0 57 54954.5 58 50189.0 59 45244.5 60 38082.0 61 30898.0 62 24890.0 63 19022.0 64 13844.0 65 10248.0 66 7576.5 67 5929.0 68 4607.0 69 3555.0 70 2719.0 71 1819.0 72 1262.5 73 939.5 74 671.0 75 487.5 76 344.0 77 238.5 78 166.0 79 116.5 80 80.5 81 64.5 82 51.5 83 43.5 84 37.0 85 29.0 86 23.0 87 17.5 88 13.0 89 12.0 90 9.0 91 6.0 92 4.5 93 3.5 94 3.5 95 2.0 96 2.5 97 2.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27120062022617686 2 0.16272037213570612 3 0.19213958253398786 4 0.15990206122360182 5 0.2323870401208808 6 0.1903101526436745 7 0.23644145231022387 8 0.2443525004845517 9 0.1460082828674385 10-11 0.20190478262417377 12-13 0.20835723129135997 14-15 0.1690739327007132 16-17 0.28264691805340747 18-19 0.22791235349727662 20-21 0.1951062255993608 22-23 0.24121280324036534 24-25 0.22929678692778402 26-27 0.3409167322624411 28-29 0.3124616808604056 30-31 0.28756660113681765 32-33 0.18756600780820454 34-35 0.3543407921332537 36-37 0.3939701990815273 38-39 0.3763681168936478 40-41 0.3390873023721278 42-43 0.3713989897591481 44-45 0.37985392249546107 46-47 0.3853174901408562 48-49 0.4050456665255863 50-51 0.39676378796808687 52-53 0.3970851743001689 54-55 0.39152271855259463 56-57 0.3701876105074542 58-59 0.34499586647732894 60-61 0.3114975218641594 62-63 0.3792605938823865 64-65 0.41221505393357094 66-67 0.41809889601322725 68-69 0.4224499725091076 70-71 0.42215330820257024 72-73 0.4141433719260634 74-75 0.40897646858720543 76-77 0.3930802061619154 78-79 0.36788846213179016 80-81 0.379087539703573 82-83 0.3880616349763262 84-85 0.38956967853455743 86-87 0.3842544430424309 88-89 0.3931049281874602 90-91 0.4126106063422873 92-93 0.4118936676014888 94-95 0.42071943072097334 96-97 0.421312759334048 98-99 0.41906305500947344 100-101 0.3932532603407288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2022488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.34541239828744 #Duplication Level Percentage of deduplicated Percentage of total 1 77.94865148393923 45.47946216720815 2 12.483360179545116 14.566935955838387 3 3.9927380288778838 6.9887384067961635 4 1.7838328776048733 4.1631385957392055 5 0.9847568650341733 2.8728022701231763 6 0.597721097756581 2.092457036865888 7 0.41009124436359934 1.674885994132475 8 0.2885000436583676 1.3466123219337112 9 0.21652333737309679 1.1369829071598172 >10 1.1903266972753384 12.687616537645694 >50 0.07183200026893874 2.8580257252797634 >100 0.030645189565435185 3.101567045415609 >500 5.955569300968E-4 0.22745057617719994 >1k 2.552386843271988E-4 0.26502807206679735 >5k 1.701591228847992E-4 0.5382963876179716 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5818 0.2876654892389967 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5026 0.24850580077607382 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3022 0.14941992239261742 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4833215326864733E-4 2 0.0 0.0 0.0 0.0 1.4833215326864733E-4 3 0.0 0.0 0.0 0.0 1.4833215326864733E-4 4 0.0 0.0 0.0 4.944405108954911E-5 1.4833215326864733E-4 5 0.0 0.0 0.0 4.944405108954911E-5 1.9777620435819645E-4 6 0.0 0.0 0.0 4.944405108954911E-5 2.4722025544774557E-4 7 0.0 0.0 0.0 4.944405108954911E-5 2.4722025544774557E-4 8 0.0 0.0 0.0 4.944405108954911E-5 2.4722025544774557E-4 9 0.0 0.0 0.0 9.888810217909822E-5 2.9666430653729466E-4 10-11 0.0 0.0 0.0 9.888810217909822E-5 3.213863320820692E-4 12-13 0.0 0.0 0.0 9.888810217909822E-5 7.416607663432366E-4 14-15 0.0 0.0 0.0 9.888810217909822E-5 8.89992919611884E-4 16-17 0.0 0.0 0.0 9.888810217909822E-5 0.001112491149514855 18-19 0.0 0.0 0.0 1.2361012772387278E-4 0.0012855453283282768 20-21 0.0 0.0 0.0 2.22498229902971E-4 0.001384433430507375 22-23 0.0 0.0 0.0 9.394369707014332E-4 0.0014833215326864734 24-25 0.0 0.0 0.0 0.002175538247940161 0.0015080435582312478 26-27 0.0 0.0 0.0 0.004227466368156449 0.0017305417881342188 28-29 0.0 0.0 0.0 0.01280600923219322 0.001779985839223768 30-31 0.0 0.0 0.0 0.039851905178176585 0.0018541519158580917 32-33 0.0 0.0 0.0 0.09602034721590437 0.0019035959669476406 34-35 0.0 0.0 0.0 0.18647823868423447 0.0020766501457610627 36-37 0.0 0.0 0.0 0.32289437564030044 0.0020766501457610627 38-39 0.0 0.0 0.0 0.4990388076468192 0.002175538247940161 40-41 0.0 0.0 0.0 0.6851956599989715 0.0022249822990297098 42-43 0.0 0.0 0.0 0.8794366147042652 0.0023485924267535826 44-45 0.0 0.0 0.0 1.1341476438920775 0.0024722025544774554 46-47 0.0 0.0 0.0 1.4315783332212602 0.002620534707746103 48-49 0.0 0.0 0.0 1.726907650379137 0.0027935888865595246 50-51 0.0 7.416607663432366E-5 0.0 2.0259947154198192 0.0028183109121042993 52-53 0.0 9.888810217909822E-5 0.0 2.3296306331607406 0.0029171990142833975 54-55 0.0 9.888810217909822E-5 0.0 2.685751411133218 0.002966643065372947 56-57 0.0 9.888810217909822E-5 0.0 3.0981889632966917 0.0030408091420072703 58-59 0.0 9.888810217909822E-5 0.0 3.535150764800582 0.0031396972441863685 60-61 0.0 9.888810217909822E-5 0.0 3.9842758028725016 0.003213863320820692 62-63 0.0 9.888810217909822E-5 0.0 4.433524451072145 0.00331275142299979 64-65 0.0 9.888810217909822E-5 0.0 4.933156587332039 0.0034610835762684377 66-67 0.0 9.888810217909822E-5 0.0 5.497560430519242 0.003510527627357987 68-69 0.0 9.888810217909822E-5 0.0 6.131556775614985 0.003559971678447536 70-71 0.0 9.888810217909822E-5 0.0 6.790670698664219 0.003658859780626634 72-73 0.0 9.888810217909822E-5 0.0 7.4563359584828195 0.003782469908350507 74-75 0.0 9.888810217909822E-5 0.0 8.150975432239894 0.003881358010529605 76-77 0.0 9.888810217909822E-5 0.0 8.901560849804795 0.003930802061619155 78-79 0.0 9.888810217909822E-5 0.0 9.726188733876295 0.0041780223170669005 80-81 0.0 9.888810217909822E-5 0.0 10.58982797425745 0.004252188393701223 82-83 0.0 9.888810217909822E-5 0.0 11.47245373025699 0.0043016324447907725 84-85 0.0 9.888810217909822E-5 0.0 12.363262476711853 0.0043263544703355476 86-87 0.0 9.888810217909822E-5 0.0 13.3071246899858 0.00444996459805942 88-89 0.0 9.888810217909822E-5 0.0 14.297736253564917 0.004548852700238518 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3950 0.0 48.3586 1 GTATCAA 6595 0.0 36.38491 1 TCAACGC 8135 0.0 28.981562 4 ATCAACG 8180 0.0 28.648153 3 CAACGCA 8280 0.0 28.416744 5 TATCAAC 8300 0.0 28.34827 2 AACGCAG 8585 0.0 27.461756 6 ACGCAGA 9835 0.0 23.683226 7 CGCAGAG 9860 0.0 23.47884 8 GTACATG 5960 0.0 22.801558 1 TACATGG 5880 0.0 22.024612 2 GAGTACT 6215 0.0 21.195103 12-13 GCAGAGT 10740 0.0 20.671144 9 ACATGGG 5880 0.0 20.653116 3 CAGAGTA 9940 0.0 19.79287 10-11 GTACTTT 6705 0.0 19.682058 14-15 AGAGTAC 8910 0.0 19.55055 10-11 GTGGTAT 2360 0.0 18.724737 1 AGTACTT 6765 0.0 18.524643 12-13 TAGGACA 785 0.0 18.12903 4 >>END_MODULE