FastQCFastQC Report
Thu 26 May 2016
SRR938762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022488
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55090.27238727745232605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51070.25251076891432733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31570.15609486928970653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.045.5972251
GTATCAA66450.037.5093571
ATCAACG83200.029.834913
TCAACGC84050.029.5896574
TATCAAC85050.029.2975542
CAACGCA85400.029.1219065
AACGCAG88650.028.0445566
CTATACG856.438906E-427.9188464
CGCAGAG98900.024.8975338
ACGCAGA99200.024.8700687
GCAGAGT110350.021.9271989
GAGTACT63950.020.29143712-13
AGAGTAC94950.020.2124810-11
AGTACTT67450.019.37919212-13
GTACTTT67700.019.06234214-15
GTACATG59300.019.0106531
CAGAGTA105600.018.84794810-11
TACATGG59900.017.986452
GTGGTAT23750.016.8236051
ACATGGG60050.016.7559573
Produced by FastQC (version 0.11.5)