FastQCFastQC Report
Thu 26 May 2016
SRR938761_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938761_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120177
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.33182256018468514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28020.25013904052663105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.16033180470586345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.041.6448821
GTATCAA41250.038.8358731
TCAACGC54050.029.1873464
ATCAACG53900.029.1806833
CAACGCA54450.028.9729295
TATCAAC54700.028.7539062
AACGCAG56650.027.993756
AGTGCGT909.122113E-426.3016198
ATAACGC950.00124557124.9340323
ACGCAGA64200.024.3351427
CGCAGAG65450.023.9427138
GTACATG43500.022.8807531
TACATGG44700.022.0445842
GCAGAGT69150.021.9085069
AGAGTAC59100.020.86773110-11
ACATGGG45650.020.7556023
AGTACTT36850.020.55594612-13
TAATACC3455.4569682E-1119.2244994
GAGTACT35650.018.99028612-13
CCGATCG1250.006147533718.9371669
Produced by FastQC (version 0.11.5)