##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938761_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1120177 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49665097569402 33.0 31.0 34.0 30.0 34.0 2 31.566092679996107 33.0 31.0 34.0 28.0 34.0 3 31.574985917404124 33.0 31.0 34.0 28.0 34.0 4 35.14051350813309 37.0 35.0 37.0 32.0 37.0 5 35.175528510226506 37.0 35.0 37.0 32.0 37.0 6 35.25556943233078 37.0 35.0 37.0 32.0 37.0 7 35.16206099571764 37.0 35.0 37.0 32.0 37.0 8 35.13743363771975 37.0 35.0 37.0 32.0 37.0 9 36.6909497338367 39.0 37.0 39.0 32.0 39.0 10-11 36.584614752847095 39.0 37.0 39.0 32.0 39.0 12-13 36.47033281347501 39.0 37.0 39.0 32.0 39.0 14-15 37.61693687693999 40.0 37.0 41.0 32.0 41.0 16-17 37.548481177528195 40.0 37.0 41.0 32.0 41.0 18-19 37.489316866888004 40.0 37.0 41.0 32.0 41.0 20-21 37.38600953242211 40.0 37.0 41.0 31.5 41.0 22-23 37.28403457667851 40.0 37.0 41.0 31.0 41.0 24-25 37.28008520082094 40.0 37.0 41.0 31.0 41.0 26-27 37.19516112185842 40.0 37.0 41.0 31.0 41.0 28-29 37.08639259688424 40.0 36.5 41.0 31.0 41.0 30-31 36.878449120094416 39.5 36.0 41.0 30.0 41.0 32-33 36.68460921800751 39.0 36.0 41.0 30.0 41.0 34-35 36.358137151539445 39.0 35.0 41.0 30.0 41.0 36-37 36.11342403923666 39.0 35.0 41.0 29.0 41.0 38-39 35.9537823040466 39.0 35.0 41.0 29.0 41.0 40-41 35.77854749740443 39.0 35.0 40.0 27.5 41.0 42-43 35.52203937413462 38.5 35.0 40.0 27.0 41.0 44-45 35.497403088976114 39.0 35.0 41.0 26.5 41.0 46-47 35.32466878002316 39.0 35.0 41.0 26.0 41.0 48-49 35.11228180903554 39.0 35.0 41.0 24.5 41.0 50-51 33.96580808211559 38.0 33.0 40.0 23.5 40.5 52-53 34.06986574443146 38.0 33.5 39.5 23.5 40.5 54-55 34.60865604275038 38.0 34.0 40.0 24.0 41.0 56-57 34.53270063570311 38.0 34.0 40.0 23.0 41.0 58-59 34.32187948868795 38.0 34.0 40.0 23.0 41.0 60-61 34.04548209791845 38.0 34.0 40.0 22.5 41.0 62-63 33.688633582014276 37.0 33.0 40.0 21.5 41.0 64-65 33.29559167881504 37.0 33.0 40.0 20.0 41.0 66-67 32.902945695189246 36.0 32.0 39.0 19.0 41.0 68-69 32.48345484686795 36.0 32.0 39.0 18.0 41.0 70-71 32.076492822116506 35.0 31.5 39.0 14.5 40.0 72-73 31.661299062558864 35.0 31.0 38.0 12.0 40.0 74-75 31.18942140393884 35.0 31.0 37.0 7.5 39.0 76-77 30.73848373962329 35.0 30.5 37.0 7.0 39.0 78-79 30.307827691516607 34.5 30.0 36.0 2.0 39.0 80-81 29.859201715443184 34.0 30.0 36.0 2.0 37.5 82-83 29.388336396837285 34.0 29.0 35.0 2.0 37.0 84-85 28.959426054989525 34.0 29.0 35.0 2.0 36.5 86-87 28.56296281748331 34.0 29.0 35.0 2.0 36.0 88-89 28.185802779382186 34.0 28.5 35.0 2.0 36.0 90-91 27.768616477574525 34.0 27.0 35.0 2.0 35.5 92-93 27.3017237454438 34.0 26.0 35.0 2.0 35.0 94-95 26.77264887602584 33.0 25.0 35.0 2.0 35.0 96-97 26.170054821693356 33.0 24.5 35.0 2.0 35.0 98-99 25.45323283731053 33.0 21.0 35.0 2.0 35.0 100-101 23.35528224557369 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42085394944598065 1101 2 0.22855043262856256 1101 3 0.47173610585821635 1101 4 0.2717988254465453 1101 5 0.21447039319977534 1101 6 0.15284921279717878 1101 7 0.28485242419826307 1101 8 0.23369560069705386 1101 9 0.4021833149279246 1101 10-11 0.2698384637354536 1101 12-13 0.2565673226692766 1101 14-15 0.5780592252804979 1101 16-17 0.43684352566989304 1101 18-19 0.5749364584426218 1101 20-21 0.7948323702528555 1101 22-23 0.6108327190591538 1101 24-25 0.6465113617119371 1101 26-27 0.5670099394341221 1101 28-29 0.5102093806189814 1101 30-31 0.5836420087535998 1101 32-33 0.4445389754681699 1101 34-35 0.609230569319628 1101 36-37 0.6920735373274951 1101 38-39 0.7774739431579505 1101 40-41 0.9673887295337309 1101 42-43 0.8586642075626116 1101 44-45 0.8915163739515322 1101 46-47 0.7973663056052445 1101 48-49 0.7204360982575722 1101 50-51 0.7275489291067103 1101 52-53 0.6010946565402193 1101 54-55 0.530783138215476 1101 56-57 0.4235869734590665 1101 58-59 0.4747839792693682 1101 60-61 0.22078428859957455 1101 62-63 0.28996674129219 1101 64-65 0.4186538236843873 1101 66-67 0.08944277729209915 1101 68-69 0.05236525594251873 1101 70-71 0.05614016690347512 1101 72-73 0.1644644091451859 1101 74-75 -0.30992753995171896 1101 76-77 -0.13977898819197065 1101 78-79 -0.3129797847021898 1101 80-81 -0.131091654743134 1101 82-83 -0.3844282376165289 1101 84-85 -0.7013770898699043 1101 86-87 -0.6434561516386452 1101 88-89 -0.5435276871330394 1101 90-91 -0.5657027505634176 1101 92-93 -0.75871398080319 1101 94-95 -0.7952888222375911 1101 96-97 -0.9024378008276592 1101 98-99 -0.9928154682192201 1101 100-101 -0.9831110247208734 1102 1 0.2792292804445289 1102 2 0.3233970684206007 1102 3 0.4835907747482544 1102 4 0.3602241822564807 1102 5 0.3729337428865094 1102 6 0.4172265270016098 1102 7 0.4770785542356606 1102 8 0.42844716145037154 1102 9 0.49648772588977863 1102 10-11 0.44094196554225107 1102 12-13 0.4794895059784139 1102 14-15 0.31684705567553806 1102 16-17 0.36655391818867855 1102 18-19 0.4752395101285316 1102 20-21 0.5654424846028689 1102 22-23 0.40174933899297827 1102 24-25 0.6995361220745622 1102 26-27 0.5959911138336835 1102 28-29 0.6362887237098889 1102 30-31 0.7384471977709524 1102 32-33 0.5747936354449195 1102 34-35 0.8658078307871264 1102 36-37 0.6455192647072394 1102 38-39 0.6483436569300878 1102 40-41 0.605224323994122 1102 42-43 0.47495379276061556 1102 44-45 0.6689302957597434 1102 46-47 0.5919893665015508 1102 48-49 0.6111267292810041 1102 50-51 0.48606390156837165 1102 52-53 0.489887500658007 1102 54-55 0.630408594557089 1102 56-57 0.668626173343192 1102 58-59 0.538359840608365 1102 60-61 0.5837072518211315 1102 62-63 0.5245616139250728 1102 64-65 0.3535627039158342 1102 66-67 0.5652422803764594 1102 68-69 0.49445861517389744 1102 70-71 0.3757612780187216 1102 72-73 0.24147519050602284 1102 74-75 0.21564151757981875 1102 76-77 0.22622555758331941 1102 78-79 0.18621987659298256 1102 80-81 -0.05020637986114451 1102 82-83 -0.09738425426951736 1102 84-85 -0.017543374627077668 1102 86-87 -0.05449599773592695 1102 88-89 -0.09481342847694307 1102 90-91 -0.33966648834943314 1102 92-93 -0.5477773802557664 1102 94-95 -0.5129958054249073 1102 96-97 -0.31760004118181584 1102 98-99 -0.6220943091950772 1102 100-101 -0.7253954624968593 1103 1 0.1355957400718566 1103 2 0.35115372376406384 1103 3 0.32044247393951864 1103 4 0.26610064088888663 1103 5 0.11409666910564908 1103 6 0.37006526373001236 1103 7 0.3718651426348245 1103 8 0.222092427735177 1103 9 0.3908958871246213 1103 10-11 0.3343418759989234 1103 12-13 0.3836861388649808 1103 14-15 0.4795236550188946 1103 16-17 0.4992059641826856 1103 18-19 0.5442491680161581 1103 20-21 0.5372443309010393 1103 22-23 0.6688291960079127 1103 24-25 0.4705388296942772 1103 26-27 0.5339379421521144 1103 28-29 0.6301583813132581 1103 30-31 0.5378257244278615 1103 32-33 0.45368450628932067 1103 34-35 0.5822592855108937 1103 36-37 0.651220545318985 1103 38-39 0.6559628348370481 1103 40-41 0.8213168947404554 1103 42-43 0.6541665931926985 1103 44-45 0.6074980644252648 1103 46-47 0.4971951384929625 1103 48-49 0.5450867474739383 1103 50-51 0.5094428830485356 1103 52-53 0.49989094736550754 1103 54-55 0.5632448795038627 1103 56-57 0.5250805773115275 1103 58-59 0.42206100040767325 1103 60-61 0.5688748612985108 1103 62-63 0.6164296938602547 1103 64-65 0.534117765956573 1103 66-67 0.4098659496631072 1103 68-69 0.2600203218981747 1103 70-71 0.25993058597670426 1103 72-73 0.3679409295227529 1103 74-75 0.2960732798778132 1103 76-77 0.36989463102306175 1103 78-79 0.3067347152017952 1103 80-81 0.39610582077276746 1103 82-83 0.38382497153601847 1103 84-85 0.3617765580022301 1103 86-87 0.36950232510330494 1103 88-89 0.3432915219899648 1103 90-91 0.3159639037934987 1103 92-93 0.4299575999167793 1103 94-95 0.30303440478871835 1103 96-97 0.4023292124816429 1103 98-99 0.25063512781900954 1103 100-101 0.037694919412544436 1104 1 0.14593408593652057 1104 2 0.1988980846663182 1104 3 0.2067206694282433 1104 4 0.17117582885880722 1104 5 0.1488711051958802 1104 6 0.12758406072249073 1104 7 0.16885762383783032 1104 8 0.01720520969006145 1104 9 0.1061214510343973 1104 10-11 0.04486819178839596 1104 12-13 0.15060343209805893 1104 14-15 0.17078305351513023 1104 16-17 0.36151799425787345 1104 18-19 0.24584691237706835 1104 20-21 0.21205636097622715 1104 22-23 0.17822769224851243 1104 24-25 0.10775687480705187 1104 26-27 0.4117574910242965 1104 28-29 0.33410574973429874 1104 30-31 0.3587843710443934 1104 32-33 0.45227473185323674 1104 34-35 0.2678795862627723 1104 36-37 0.3800739287776267 1104 38-39 0.4040831355889267 1104 40-41 0.19349734586827338 1104 42-43 0.5286966683806611 1104 44-45 0.471689793748574 1104 46-47 0.5996387475155203 1104 48-49 0.6423611835641552 1104 50-51 0.6537098003417725 1104 52-53 0.743311999997097 1104 54-55 0.8569478870226632 1104 56-57 0.6674677953566359 1104 58-59 0.7204632560467701 1104 60-61 0.5557169665616684 1104 62-63 0.7926514983715336 1104 64-65 0.7211478411445427 1104 66-67 0.6067644459036998 1104 68-69 0.717257164003442 1104 70-71 0.7448929919917404 1104 72-73 0.8007416814024495 1104 74-75 0.702088317471798 1104 76-77 0.5371878641057712 1104 78-79 0.5835204294875069 1104 80-81 0.3697900312990825 1104 82-83 0.3617385053706066 1104 84-85 0.4308555053706513 1104 86-87 0.43106247547924426 1104 88-89 0.5829531761252973 1104 90-91 0.5058135278536469 1104 92-93 0.31012677284911305 1104 94-95 0.5492750062924792 1104 96-97 0.5682126711282613 1104 98-99 0.2943381353378065 1104 100-101 0.20545807730728072 1105 1 0.19153278830421527 1105 2 0.2153718242129763 1105 3 0.2546573233906564 1105 4 0.4741039748908591 1105 5 0.43232093261631377 1105 6 0.4400403970555047 1105 7 0.23321700434982517 1105 8 0.19182010479350708 1105 9 0.2669351688616288 1105 10-11 0.32084807364575596 1105 12-13 0.36386413099341297 1105 14-15 0.49525078909142195 1105 16-17 0.23148975553779394 1105 18-19 0.28585925925161604 1105 20-21 0.38741699692672427 1105 22-23 0.4298873439075592 1105 24-25 0.3570889503962107 1105 26-27 0.3901167401129797 1105 28-29 0.4653046935058782 1105 30-31 0.40252628678130264 1105 32-33 0.4234683841071458 1105 34-35 0.5841190983364228 1105 36-37 0.5329646364575922 1105 38-39 0.5795146709590924 1105 40-41 0.5040670659366171 1105 42-43 0.6000366155346626 1105 44-45 0.6950644677841638 1105 46-47 0.7678601577568571 1105 48-49 0.660309544577089 1105 50-51 0.9349111650691384 1105 52-53 0.7514892105026618 1105 54-55 0.7559983535754284 1105 56-57 0.6986410597042578 1105 58-59 0.6453029541881605 1105 60-61 0.8066192049082233 1105 62-63 0.5899068262035314 1105 64-65 0.5405769624759316 1105 66-67 0.5973962116105227 1105 68-69 0.693503696848893 1105 70-71 0.6152473507497263 1105 72-73 0.5812063838669843 1105 74-75 0.5494812074704853 1105 76-77 0.46113094397655985 1105 78-79 0.559434513224911 1105 80-81 0.4293105264191297 1105 82-83 0.3656933095844508 1105 84-85 0.4925449174934151 1105 86-87 0.44111765597879327 1105 88-89 0.5356935251579564 1105 90-91 0.6064591448861094 1105 92-93 0.3379924063440747 1105 94-95 0.37161446233829665 1105 96-97 0.1335862591922563 1105 98-99 0.07369525052688175 1105 100-101 0.2882379052119113 1106 1 0.3262833235164493 1106 2 0.26925329512236473 1106 3 0.3675350886538773 1106 4 0.4010700738169959 1106 5 0.3647044970868194 1106 6 0.40287216415865146 1106 7 0.34446720854726465 1106 8 0.2002949433700607 1106 9 0.20985274187832914 1106 10-11 0.3733298248429975 1106 12-13 0.2866207534387968 1106 14-15 0.4343010775471754 1106 16-17 0.38130490311881005 1106 18-19 0.5316437643200018 1106 20-21 0.36777363312856437 1106 22-23 0.48197918546754437 1106 24-25 0.19270786208834068 1106 26-27 0.1880012546389409 1106 28-29 0.19185379976592998 1106 30-31 0.3875850526549698 1106 32-33 0.581129872040151 1106 34-35 0.6439467986863576 1106 36-37 0.6862971386715273 1106 38-39 0.6144311434590719 1106 40-41 0.7069486142260857 1106 42-43 0.4762293435264695 1106 44-45 0.5724557272739688 1106 46-47 0.76421168687326 1106 48-49 0.7073574944347953 1106 50-51 0.7741484553906091 1106 52-53 0.6172588413996678 1106 54-55 0.5001054304138464 1106 56-57 0.43361212815123906 1106 58-59 0.37762719392885913 1106 60-61 0.3929938620714708 1106 62-63 0.13810859906719486 1106 64-65 0.3135824908529301 1106 66-67 0.22398916658391244 1106 68-69 0.572136476771334 1106 70-71 0.5449316400136652 1106 72-73 0.3663528471673345 1106 74-75 0.30924786353321565 1106 76-77 0.02223283634946327 1106 78-79 0.10720639674209309 1106 80-81 0.09675844532480227 1106 82-83 0.11431035555681746 1106 84-85 0.0630106596113933 1106 86-87 0.18921281628453102 1106 88-89 0.2680719309563031 1106 90-91 0.23713862798715724 1106 92-93 0.07476471115281313 1106 94-95 0.002903528532659294 1106 96-97 0.16839010068345672 1106 98-99 0.0869414309600387 1106 100-101 0.6627880260108192 1107 1 -0.062956383480266 1107 2 0.07800971509373866 1107 3 0.024030836738408112 1107 4 0.023036900456723686 1107 5 -0.05183166524373206 1107 6 0.028737281612556842 1107 7 -0.14292859847471107 1107 8 -0.05791841017039445 1107 9 0.033697147031148234 1107 10-11 -0.042987426988275956 1107 12-13 0.017836548673280106 1107 14-15 0.06978562751770312 1107 16-17 -0.09450127689297716 1107 18-19 0.09448370706649456 1107 20-21 0.02758250742342483 1107 22-23 -0.10951277378142521 1107 24-25 -0.06427201498499358 1107 26-27 0.07703047077095704 1107 28-29 -0.020194697329294797 1107 30-31 0.04355457357775805 1107 32-33 0.17918914626765314 1107 34-35 0.018784720720539383 1107 36-37 0.28357338688235245 1107 38-39 0.12470309765625842 1107 40-41 0.15176632913725996 1107 42-43 0.1476994117255117 1107 44-45 0.1749479391119806 1107 46-47 0.2295606807269266 1107 48-49 0.3185050906291238 1107 50-51 0.4634681489159078 1107 52-53 0.5692374555278121 1107 54-55 0.469142224217002 1107 56-57 0.2429368734572961 1107 58-59 0.28722136754172567 1107 60-61 0.22505162076473795 1107 62-63 0.12033896026952107 1107 64-65 0.2283000121125056 1107 66-67 0.3406684627024603 1107 68-69 0.18695082382867412 1107 70-71 0.4297131504961129 1107 72-73 0.1009491256888424 1107 74-75 0.3027479056313851 1107 76-77 0.29858629260946046 1107 78-79 0.3304908961948172 1107 80-81 0.18140945147113285 1107 82-83 0.08479953632637205 1107 84-85 0.24356972334802762 1107 86-87 0.3840894686378178 1107 88-89 0.3503022921586272 1107 90-91 0.22230323184335177 1107 92-93 0.34578432890140576 1107 94-95 0.21347858957500776 1107 96-97 0.23634076162477058 1107 98-99 0.429763692473891 1107 100-101 0.2662358663745472 1108 1 -0.07342141770509159 1108 2 -0.09341314379648225 1108 3 -0.11514306300990285 1108 4 -0.04685701434581091 1108 5 -0.02533153587154402 1108 6 -0.050476135459867066 1108 7 -0.05805103161280556 1108 8 0.029826514911341917 1108 9 0.02001192133548102 1108 10-11 -0.006758508775028815 1108 12-13 0.08565933229240841 1108 14-15 -0.13368978000470122 1108 16-17 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2316 64-65 -3.116988057111733 2316 66-67 -2.942187721749473 2316 68-69 -3.156772991171735 2316 70-71 -3.0634376751015253 2316 72-73 -2.868281274924538 2316 74-75 -2.95121715365282 2316 76-77 -2.913100089821601 2316 78-79 -2.8842247312867677 2316 80-81 -2.4847232465764577 2316 82-83 -2.5261194373585596 2316 84-85 -2.812645534462977 2316 86-87 -2.8335451872947104 2316 88-89 -2.6752255152840725 2316 90-91 -2.9901971104442246 2316 92-93 -3.0429667321900844 2316 94-95 -2.992088375566425 2316 96-97 -2.8394820977087285 2316 98-99 -3.1770964351309203 2316 100-101 -3.549171182011225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4267.0 3 1217.0 4 1213.0 5 1324.0 6 1529.0 7 1807.0 8 2147.0 9 2729.0 10 3349.0 11 4178.0 12 5450.0 13 6780.0 14 7094.0 15 6936.0 16 8245.0 17 10303.0 18 9166.0 19 6929.0 20 7236.0 21 7810.0 22 8802.0 23 9957.0 24 11607.0 25 13177.0 26 15269.0 27 18013.0 28 21044.0 29 24544.0 30 29423.0 31 35336.0 32 43803.0 33 54569.0 34 71067.0 35 97129.0 36 141639.0 37 207345.0 38 187749.0 39 29993.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.624888781582676 19.68845429873155 14.336467568933065 23.35018935075271 2 15.37188517016626 21.137503775415922 39.89546789243326 23.595143161984556 3 18.443085094025708 25.462890984504476 30.84450687210609 25.249517049363718 4 12.772080087794869 17.198643040219057 37.97047647304848 32.0588003989376 5 13.529281890431017 37.46412223156815 34.60954350874966 14.397052369251176 6 30.53418685108747 36.600586912529316 18.433090006947932 14.432136229435288 7 27.1576006773146 33.25896923862837 22.082474484601452 17.500955599455576 8 25.580903098120082 34.68601268756492 20.978780525489896 18.7543036888251 9 26.380978494345154 16.592105274909592 21.346231307925002 35.68068492282025 10-11 24.263152771042158 26.72861354561035 28.423936002515028 20.58429768083246 12-13 25.11659001177623 24.538630447510045 28.501730707998234 21.843048832715496 14-15 22.595703197393107 25.720126843606444 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21.80999674920692 40-41 21.966536574956944 27.014336985046178 29.194874870782446 21.824251569214436 42-43 21.886489223027525 27.023482127763188 28.90386394383338 22.18616470537591 44-45 21.89218423608825 27.161485964703004 28.833954144576516 22.112375654632228 46-47 22.007508863634122 27.245595986901645 28.53439933126986 22.212495818194373 48-49 22.014510655589913 27.31866838541148 28.397772808129414 22.269048150869196 50-51 21.901242861941554 27.37821293916466 28.36683375392921 22.353710444964577 52-53 22.0453991951019 27.478438947990195 28.22628643087838 22.249875426029526 54-55 21.885174734100282 27.30954691656785 28.274795630795396 22.530482718536472 56-57 21.828735816688802 27.596668014789294 28.17583763701305 22.398758531508854 58-59 21.923847803195116 27.26544873240308 28.26154102021906 22.549162444182745 60-61 21.92749541216566 27.205913656845677 28.39935557452682 22.467235356461845 62-63 21.8291114606669 27.154894867209656 28.521092025046052 22.49490164707739 64-65 21.75892989310594 27.122831728202897 28.421215533057314 22.697022845633853 66-67 21.663810737098974 27.26364566137946 28.16164860403359 22.91089499748798 68-69 21.670773112440646 27.045790608950316 28.346409357312822 22.937026921296212 70-71 21.619030614716813 26.909853377380532 28.381866598717703 23.08924940918495 72-73 21.70958550663789 26.81283653699507 28.30633860309224 23.171239353274807 74-75 21.512837902087114 27.035351550936266 28.161931058734456 23.289879488242164 76-77 21.55326665751825 26.859886613444406 28.333275034598053 23.253571694439287 78-79 21.517650824906724 26.905750181354836 28.254770147872566 23.321828845865873 80-81 21.48867493842809 26.83266384763794 28.230266612085536 23.448394601848435 82-83 21.57037541927499 26.75395957023192 28.251600868145864 23.424064142347223 84-85 21.497886407224108 26.573378909989692 28.24869321358923 23.68004146919697 86-87 21.33177271473641 26.442305320677974 28.453664650867644 23.772257313717972 88-89 21.55538332920708 26.210787110293037 28.36940219516833 23.864427365331554 90-91 21.402143847416262 26.208420290615607 28.58769711999275 23.801738741975374 92-93 21.574233262569418 26.172841278164093 28.395019643602833 23.85790581566366 94-95 21.454273549512244 25.833591999239253 28.505580903817552 24.206553547430953 96-97 21.55527356094156 25.6172071139795 28.51584560032332 24.311673724755615 98-99 21.397507225887246 25.630459783519417 28.431646066982314 24.540386923611024 100-101 21.66334049394786 25.16707851412016 28.776431861296352 24.39314913063562 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 879.0 1 526.5 2 218.5 3 280.0 4 277.5 5 290.0 6 410.5 7 715.5 8 1093.5 9 1305.5 10 1276.5 11 1197.5 12 1258.5 13 1363.5 14 1493.0 15 1615.5 16 1740.0 17 1820.5 18 1898.0 19 2054.0 20 2181.5 21 2449.5 22 2852.5 23 3251.5 24 3780.0 25 4681.0 26 6100.5 27 7959.5 28 9553.5 29 11114.0 30 13257.5 31 15792.0 32 18314.0 33 20683.5 34 23613.0 35 26297.0 36 29115.0 37 31921.0 38 33324.0 39 33776.5 40 34313.5 41 35644.0 42 36729.5 43 38177.0 44 40026.5 45 40877.5 46 41106.0 47 41562.0 48 43006.0 49 43487.0 50 41792.0 51 40061.0 52 38404.0 53 37169.5 54 35758.0 55 32972.5 56 29774.5 57 26521.5 58 23651.0 59 20769.0 60 17602.5 61 14382.5 62 11401.0 63 8784.0 64 6559.0 65 4862.0 66 3518.5 67 2693.0 68 2036.5 69 1417.5 70 1026.0 71 755.0 72 563.5 73 422.5 74 317.0 75 229.0 76 157.5 77 102.5 78 67.0 79 40.0 80 27.5 81 24.5 82 22.5 83 19.0 84 16.5 85 9.0 86 4.5 87 3.5 88 2.5 89 3.0 90 3.5 91 3.5 92 3.5 93 1.5 94 0.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1680091628376587 2 0.09775240877111385 3 0.0906106802764206 4 0.10757228545131706 5 0.1292652857539478 6 0.037404802990955895 7 0.041154210450669845 8 0.04445725987946548 9 0.04624269200313879 10-11 0.045349975941302134 12-13 0.04896547599174059 14-15 0.04735858708043461 16-17 0.04418944506091448 18-19 0.04883156858246509 20-21 0.052000710601985224 22-23 0.06681979722847371 24-25 0.08275477893225802 26-27 0.08529901970849249 28-29 0.07467569857263628 30-31 0.06302575396566792 32-33 0.0510187229339649 34-35 0.30097922024822865 36-37 0.47501421650328474 38-39 0.45211604951717455 40-41 0.4293964257434316 42-43 0.4586328767685821 44-45 0.46760467319004057 46-47 0.4667119571282038 48-49 0.482959389453631 50-51 0.472291432514683 52-53 0.48005806225266184 54-55 0.4716218954683054 56-57 0.45046452480277677 58-59 0.43015523439599274 60-61 0.3976603697451385 62-63 0.456267179204715 64-65 0.49295780934620154 66-67 0.4942968834389565 68-69 0.5060807354552004 70-71 0.5012600687212825 72-73 0.49233290810291586 74-75 0.48483409318348797 76-77 0.46689050034057117 78-79 0.4427871666709815 80-81 0.4479202840265422 82-83 0.4603736730891636 84-85 0.45818651873766375 86-87 0.4672922225683977 88-89 0.478942167175366 90-91 0.4825576672258045 92-93 0.48541435862368176 94-95 0.4894762167050386 96-97 0.49085992660088545 98-99 0.48519117960822267 100-101 0.4715326238621218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1120177.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.36458152485784 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9295302015455 57.37807209806576 2 9.92059859325534 13.564351426079895 3 2.742988898729508 5.625698645669205 4 1.1187786481581794 3.0593933640112034 5 0.5731223734149128 1.9590635610521914 6 0.36259068682271356 1.4873016341667358 7 0.24719241028056943 1.182944397946647 8 0.16793484063822867 0.9184636082940956 9 0.12811961684944875 0.788295959193392 >10 0.7462442928392624 9.37209944770185 >50 0.04676329302611858 2.192656739387289 >100 0.015349017896648911 1.5742613899968068 >500 3.935632717979768E-4 0.15165896050206054 >1k 3.935632717979768E-4 0.7457387679329588 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3717 0.33182256018468514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2802 0.25013904052663105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1796 0.16033180470586345 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.927160618366562E-5 0.0 14-15 0.0 0.0 0.0 8.927160618366562E-5 0.0 16-17 0.0 0.0 0.0 2.6781481855099685E-4 0.0 18-19 0.0 0.0 0.0 3.570864247346625E-4 0.0 20-21 0.0 0.0 0.0 4.0172222782649527E-4 0.0 22-23 0.0 0.0 0.0 7.588086525611577E-4 0.0 24-25 0.0 0.0 0.0 0.0017854321236733123 0.0 26-27 0.0 0.0 0.0 0.0033030494287956277 0.0 28-29 0.0 0.0 0.0 0.010221598908029712 0.0 30-31 0.0 0.0 0.0 0.027942012735487336 0.0 32-33 0.0 0.0 0.0 0.06798032810886137 0.0 34-35 0.0 0.0 0.0 0.13448767471569223 0.0 36-37 0.0 0.0 0.0 0.24143505892372366 0.0 38-39 0.0 0.0 0.0 0.40207931425122995 0.0 40-41 0.0 0.0 0.0 0.576114310506286 0.0 42-43 0.0 0.0 0.0 0.7505510289891687 0.0 44-45 0.0 0.0 0.0 0.9668114949690987 0.0 46-47 0.0 0.0 0.0 1.223511998550229 0.0 48-49 0.0 0.0 0.0 1.495344039379491 0.0 50-51 0.0 0.0 0.0 1.7812809939857719 0.0 52-53 0.0 0.0 0.0 2.0757880227856846 0.0 54-55 0.0 0.0 0.0 2.4048877989817683 0.0 56-57 0.0 0.0 0.0 2.7846938474901735 0.0 58-59 0.0 0.0 0.0 3.1819971308105774 0.0 60-61 0.0 0.0 0.0 3.6144734269673453 0.0 62-63 0.0 0.0 0.0 4.053288007163154 0.0 64-65 0.0 0.0 0.0 4.515893470406908 0.0 66-67 0.0 0.0 0.0 5.042506675284352 0.0 68-69 0.0 0.0 0.0 5.604203621391976 0.0 70-71 0.0 0.0 0.0 6.2092419323017705 0.0 72-73 0.0 0.0 0.0 6.826331910046359 0.0 74-75 0.0 0.0 0.0 7.4649363448812105 0.0 76-77 0.0 0.0 0.0 8.152059897676885 0.0 78-79 0.0 0.0 0.0 8.878820043618106 0.0 80-81 0.0 0.0 0.0 9.670703826270312 0.0 82-83 0.0 0.0 0.0 10.47383583130166 0.0 84-85 0.0 0.0 0.0 11.290224669851282 0.0 86-87 0.0 0.0 0.0 12.147365996623748 0.0 88-89 0.0 0.0 0.0 13.05655266980129 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2390 0.0 41.644882 1 GTATCAA 4125 0.0 38.835873 1 TCAACGC 5405 0.0 29.187346 4 ATCAACG 5390 0.0 29.180683 3 CAACGCA 5445 0.0 28.972929 5 TATCAAC 5470 0.0 28.753906 2 AACGCAG 5665 0.0 27.99375 6 AGTGCGT 90 9.122113E-4 26.301619 8 ATAACGC 95 0.001245571 24.934032 3 ACGCAGA 6420 0.0 24.335142 7 CGCAGAG 6545 0.0 23.942713 8 GTACATG 4350 0.0 22.880753 1 TACATGG 4470 0.0 22.044584 2 GCAGAGT 6915 0.0 21.908506 9 AGAGTAC 5910 0.0 20.867731 10-11 ACATGGG 4565 0.0 20.755602 3 AGTACTT 3685 0.0 20.555946 12-13 TAATACC 345 5.4569682E-11 19.224499 4 GAGTACT 3565 0.0 18.990286 12-13 CCGATCG 125 0.0061475337 18.937166 9 >>END_MODULE