FastQCFastQC Report
Thu 26 May 2016
SRR938761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120177
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.31477168340360495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29330.26183362093669127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.17452599008906627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.042.413761
GTATCAA45850.033.5709151
TCAACGC56150.027.1238484
ATCAACG56200.027.0152953
CAACGCA57150.026.649245
TATCAAC58150.026.3541372
AACGCAG58600.026.1517646
GTACATG44000.023.6104241
GTATAGG2853.6379788E-1223.4090161
ACGCAGA67100.022.6268187
TACATGG45600.022.5782852
CGCAGAG68100.022.0158798
GAGTACT35600.021.05732212-13
ACATGGG45800.021.0293873
TATATGG3001.6916601E-1020.5597652
GCAGAGT70900.020.477239
AGTACTT36850.019.31300412-13
AGAGTAC61500.018.97824510-11
CAGAGTA69150.018.6283210-11
GTACTTT39900.018.55016514-15
Produced by FastQC (version 0.11.5)