##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938761_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1120177 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.949509764974643 31.0 31.0 34.0 30.0 34.0 2 32.17364130847179 34.0 31.0 34.0 30.0 34.0 3 32.26459479171595 34.0 31.0 34.0 30.0 34.0 4 35.91551424462384 37.0 35.0 37.0 35.0 37.0 5 35.82040427539577 37.0 35.0 37.0 35.0 37.0 6 35.838615683057235 37.0 35.0 37.0 35.0 37.0 7 35.76244022150071 37.0 35.0 37.0 33.0 37.0 8 35.736590735214165 37.0 35.0 37.0 33.0 37.0 9 37.37864283947983 39.0 37.0 39.0 34.0 39.0 10-11 37.30533478191393 39.0 37.0 39.0 33.5 39.0 12-13 37.23784767942924 39.0 37.0 39.0 33.0 39.0 14-15 38.46349326936725 40.0 38.0 41.0 33.5 41.0 16-17 38.39850487913964 40.0 38.0 41.0 33.0 41.0 18-19 38.412085322230325 40.0 38.0 41.0 33.5 41.0 20-21 38.47559492830151 40.0 38.0 41.0 34.0 41.0 22-23 38.424579776231795 40.0 38.0 41.0 34.0 41.0 24-25 38.360646576389264 40.0 38.0 41.0 34.0 41.0 26-27 38.20060401168744 40.0 38.0 41.0 33.0 41.0 28-29 38.04106717063464 40.0 37.0 41.0 33.0 41.0 30-31 37.82497899885465 40.0 37.0 41.0 33.0 41.0 32-33 37.62248823177052 40.0 37.0 41.0 32.0 41.0 34-35 37.59224970696595 40.0 37.0 41.0 32.0 41.0 36-37 37.51397993352836 40.0 37.0 41.0 32.0 41.0 38-39 37.424780190987676 40.0 37.0 41.0 32.0 41.0 40-41 37.276008166566534 40.0 36.0 41.0 31.0 41.0 42-43 37.10676348469929 40.0 36.0 41.0 31.0 41.0 44-45 36.865923421030786 40.0 36.0 41.0 30.5 41.0 46-47 36.58819900783537 39.0 36.0 41.0 30.0 41.0 48-49 36.32680594227519 39.0 35.0 41.0 30.0 41.0 50-51 36.10245836149109 39.0 35.0 41.0 29.0 41.0 52-53 35.907890895813786 39.0 35.0 41.0 28.5 41.0 54-55 35.673699781373834 39.0 35.0 40.0 28.0 41.0 56-57 35.427444055716194 38.0 35.0 40.0 28.0 41.0 58-59 35.13181175832034 38.0 34.0 40.0 27.0 41.0 60-61 34.793831242741106 38.0 34.0 40.0 26.0 41.0 62-63 34.43207189578075 37.0 34.0 40.0 26.0 41.0 64-65 34.07744490379645 37.0 33.0 39.5 26.0 41.0 66-67 33.69302351324835 36.0 33.0 39.0 25.5 40.5 68-69 33.255818500112035 36.0 32.5 39.0 24.5 40.0 70-71 32.803430172196 35.0 32.0 38.0 23.5 40.0 72-73 32.46311207960885 35.0 32.0 37.5 22.5 40.0 74-75 32.042467842135665 35.0 31.5 37.0 22.0 39.0 76-77 29.968478195856548 33.0 29.0 35.0 19.0 37.5 78-79 31.044307283581077 34.0 31.0 36.0 20.0 38.0 80-81 31.100716672454443 34.0 31.0 36.0 20.0 37.5 82-83 30.807863400159082 34.0 31.0 35.0 19.0 37.0 84-85 30.435338790209045 34.0 31.0 35.0 17.5 36.5 86-87 29.91295705946471 34.0 30.0 35.0 10.0 36.0 88-89 29.42923618321033 34.0 29.5 35.0 6.5 36.0 90-91 29.039483492340942 34.0 29.0 35.0 2.0 35.0 92-93 28.77306220356247 34.0 29.0 35.0 2.0 35.0 94-95 28.416828769024896 34.0 29.0 35.0 2.0 35.0 96-97 27.938823953714458 34.0 29.0 35.0 2.0 35.0 98-99 27.23911890710129 33.0 27.0 35.0 2.0 35.0 100-101 24.209513317984566 30.0 20.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11154795859463817 1101 2 0.09525229139079272 1101 3 0.10590224582943364 1101 4 0.17745954842337852 1101 5 0.19556812559406467 1101 6 0.253663254251407 1101 7 0.09745653563152246 1101 8 0.07228701545580662 1101 9 0.25255366965387793 1101 10-11 0.13517839840822177 1101 12-13 0.0908637409246964 1101 14-15 0.18901564151322248 1101 16-17 0.20802943317735156 1101 18-19 0.1899226339222011 1101 20-21 0.24005385430630355 1101 22-23 0.1688622568422673 1101 24-25 0.16799888436896282 1101 26-27 0.28906348535052473 1101 28-29 0.17512473931512318 1101 30-31 0.22209450214311488 1101 32-33 0.23526338858865614 1101 34-35 0.09192131879601106 1101 36-37 0.13048234457243524 1101 38-39 0.11392559297692628 1101 40-41 0.3290215952774034 1101 42-43 0.3132755544851378 1101 44-45 0.3509291142242077 1101 46-47 0.49082906476257193 1101 48-49 0.40656863404186794 1101 50-51 0.5246446441044981 1101 52-53 0.4234795657205197 1101 54-55 0.6299013149694233 1101 56-57 0.37795723654599556 1101 58-59 0.4259315479216639 1101 60-61 0.2874105770413138 1101 62-63 0.2036332471200808 1101 64-65 0.3180372008834169 1101 66-67 0.5059677248206285 1101 68-69 0.34685993239732227 1101 70-71 0.09718523901625531 1101 72-73 0.2122597401656634 1101 74-75 0.27738732708621683 1101 76-77 0.36801472465294793 1101 78-79 0.30116245491184657 1101 80-81 0.45002522199169803 1101 82-83 0.35183384128179185 1101 84-85 0.3260198810934014 1101 86-87 0.5767391461715619 1101 88-89 0.6804790178293771 1101 90-91 0.7083897505430805 1101 92-93 0.6198800025494222 1101 94-95 0.6721879754871196 1101 96-97 0.6190585259410177 1101 98-99 0.7135627599595047 1101 100-101 1.0344448668320894 1102 1 -0.0337367280105525 1102 2 0.04315414126879347 1102 3 0.0981458310497203 1102 4 -0.0192937875591781 1102 5 0.04239146800161819 1102 6 0.1392519581483711 1102 7 0.10493441025874262 1102 8 0.014402657899381666 1102 9 -0.029269542768268764 1102 10-11 0.01847100298147808 1102 12-13 0.06615165859565053 1102 14-15 0.09390903491218694 1102 16-17 0.23844137549354372 1102 18-19 0.12471452728960486 1102 20-21 0.18617026180683638 1102 22-23 0.17967704249634409 1102 24-25 -0.04100928985604213 1102 26-27 0.04294540660907842 1102 28-29 -0.07008344159484636 1102 30-31 -0.10549007193185389 1102 32-33 -0.13943532381306056 1102 34-35 -0.06524310531293764 1102 36-37 -0.07141934189533572 1102 38-39 -0.07726436769320344 1102 40-41 -0.017637408440180025 1102 42-43 -0.16073664928740783 1102 44-45 -0.2301047154057798 1102 46-47 -0.4306253976363692 1102 48-49 -0.3393721074691598 1102 50-51 -0.37089722790815927 1102 52-53 -0.2935682057725657 1102 54-55 -0.19538623131466437 1102 56-57 -0.22830861703101846 1102 58-59 -0.1886283029293736 1102 60-61 -0.229890480297108 1102 62-63 -0.2526703022242387 1102 64-65 -0.24179229540457925 1102 66-67 -0.27470325983737354 1102 68-69 -0.11247970460915013 1102 70-71 -0.16845200514460856 1102 72-73 -0.22659698249376703 1102 74-75 -0.23513862357132354 1102 76-77 -0.17372080332919282 1102 78-79 -0.3531339340936164 1102 80-81 -0.2123200489535897 1102 82-83 -0.1605593385697297 1102 84-85 -0.1596473711265638 1102 86-87 -0.012311997514302675 1102 88-89 -0.09878533905855136 1102 90-91 -0.13507346915825025 1102 92-93 0.010050691954866409 1102 94-95 0.2461728823247249 1102 96-97 0.33279166961945705 1102 98-99 0.387067277880778 1102 100-101 0.8624351810625193 1103 1 0.010873261045261984 1103 2 -0.03894678685492181 1103 3 -0.05339026343981601 1103 4 0.08743155604557984 1103 5 0.08069966761217273 1103 6 0.03218372044636908 1103 7 0.0012613571154886927 1103 8 0.010982600193678138 1103 9 -0.04170789289690191 1103 10-11 -0.11686378957647747 1103 12-13 0.016364501667418097 1103 14-15 0.08538198714153111 1103 16-17 0.02276365330528307 1103 18-19 0.07268701048246129 1103 20-21 -0.06439649061296393 1103 22-23 0.015909577993873825 1103 24-25 0.016800345290427288 1103 26-27 0.1866651146693954 1103 28-29 0.2575074519907261 1103 30-31 0.061335901318848585 1103 32-33 -0.032754396542145514 1103 34-35 -0.1768258191864689 1103 36-37 -0.21085109708671723 1103 38-39 0.2778157949061111 1103 40-41 0.01646989431576884 1103 42-43 0.0011226895132807613 1103 44-45 -0.07963372460173446 1103 46-47 -0.26801364614042456 1103 48-49 -0.036721873661576865 1103 50-51 -0.13085892326185444 1103 52-53 -0.06696406545958666 1103 54-55 -0.10572120153795339 1103 56-57 -0.02290616951336233 1103 58-59 -0.2881990634791336 1103 60-61 -0.190127201589938 1103 62-63 -0.018979091683711147 1103 64-65 0.07182521955484589 1103 66-67 -0.13441515772955626 1103 68-69 -0.19336895514570074 1103 70-71 -0.21454016979063084 1103 72-73 -0.08948553658219538 1103 74-75 -0.033529330963624204 1103 76-77 -0.05478096791836151 1103 78-79 -0.13072891398190123 1103 80-81 0.06586122654138293 1103 82-83 0.1134139334572204 1103 84-85 -0.06499172207955795 1103 86-87 0.17800932801122826 1103 88-89 0.2304969285196563 1103 90-91 0.178768792450402 1103 92-93 0.23481954935356342 1103 94-95 0.11029176886053449 1103 96-97 0.10547686796503442 1103 98-99 0.28875349957868934 1103 100-101 0.8643939413848756 1104 1 -0.02390117504496203 1104 2 -0.04364603497521813 1104 3 -0.03177372208642737 1104 4 0.026341330481670866 1104 5 0.04498538189788803 1104 6 0.008687479844866175 1104 7 -0.017535635365717894 1104 8 -0.04258882837774536 1104 9 -0.03794849440065917 1104 10-11 -0.0031419850651985826 1104 12-13 -0.12508286675363678 1104 14-15 -0.047136809850954364 1104 16-17 0.10218094653836118 1104 18-19 0.05013061950501907 1104 20-21 -0.15838145301898976 1104 22-23 0.15218777348259493 1104 24-25 0.04170636032802122 1104 26-27 0.03675909963180857 1104 28-29 -0.060631645753638 1104 30-31 0.035020111845163626 1104 32-33 -0.04967168977523784 1104 34-35 0.06800500788120445 1104 36-37 0.16602860216139703 1104 38-39 0.22706391520686253 1104 40-41 0.18846237551877465 1104 42-43 0.26240088500200187 1104 44-45 0.19950161374413256 1104 46-47 0.1389412410776245 1104 48-49 0.2485224872406846 1104 50-51 0.33013731733964846 1104 52-53 0.06555473153289171 1104 54-55 -0.015495637628177406 1104 56-57 0.19184946958438331 1104 58-59 0.3222332673479329 1104 60-61 0.343237460064195 1104 62-63 0.08910361508321074 1104 64-65 0.37398687369018546 1104 66-67 0.29509612046593503 1104 68-69 0.28642427793700875 1104 70-71 0.06600494299132009 1104 72-73 0.0933152152975012 1104 74-75 0.175117285577727 1104 76-77 0.227173919299684 1104 78-79 0.35235379278501355 1104 80-81 0.3713123543609349 1104 82-83 0.3032635575173721 1104 84-85 0.4096323380708178 1104 86-87 0.41485143327438934 1104 88-89 0.46874880821890486 1104 90-91 0.4151409729015292 1104 92-93 0.5365112786768691 1104 94-95 0.453336881642489 1104 96-97 0.38884152961917096 1104 98-99 0.6083023717591409 1104 100-101 1.226236046648033 1105 1 -0.09146659272900592 1105 2 -0.10178701760658981 1105 3 -0.024024934293578326 1105 4 -0.10881890220677093 1105 5 -0.01954692402792091 1105 6 -0.03518802077420702 1105 7 0.004974930377066755 1105 8 -0.16261808735776384 1105 9 0.09234994027849552 1105 10-11 0.02155435790815119 1105 12-13 0.009078289356509117 1105 14-15 -0.043412428751004484 1105 16-17 0.04364655859057365 1105 18-19 -0.09950519957349968 1105 20-21 0.10214884005500835 1105 22-23 -0.10792014926816051 1105 24-25 -0.06687955939307955 1105 26-27 0.011071029410253175 1105 28-29 0.08104268727304742 1105 30-31 0.11416375061089212 1105 32-33 0.16695024685105864 1105 34-35 0.06899543818880005 1105 36-37 0.1532347154206306 1105 38-39 0.07719032368436274 1105 40-41 0.10677457160235804 1105 42-43 0.007667484112474199 1105 44-45 0.18034191020745993 1105 46-47 0.4219578903364649 1105 48-49 0.40312645345796483 1105 50-51 0.363022110970455 1105 52-53 0.40393215508257185 1105 54-55 0.32350431741121355 1105 56-57 0.22843746095195172 1105 58-59 0.10141226936077885 1105 60-61 0.2799126058403161 1105 62-63 0.20129719124993528 1105 64-65 0.32750884559325755 1105 66-67 0.4928112569732619 1105 68-69 0.34644106899469307 1105 70-71 0.3986905265454226 1105 72-73 0.15215351966006807 1105 74-75 0.11894851729087463 1105 76-77 0.09958609048200984 1105 78-79 0.07825663637052216 1105 80-81 0.23049832526796266 1105 82-83 0.22187232068563745 1105 84-85 0.3287296155330992 1105 86-87 0.30500704885427865 1105 88-89 0.38800301549422045 1105 90-91 0.4560667982745308 1105 92-93 0.5202420234320435 1105 94-95 0.5928077125836886 1105 96-97 0.422024187275273 1105 98-99 0.8678906536413997 1105 100-101 1.3280039496161287 1106 1 -0.08894228248646385 1106 2 -0.04536060176602774 1106 3 -0.06958202789768819 1106 4 -0.06498394532466989 1106 5 -0.011328299501876415 1106 6 -0.10265571458982237 1106 7 0.0034238896149574316 1106 8 -0.07557667181812633 1106 9 -0.07269833629511879 1106 10-11 -0.06937239754499558 1106 12-13 0.1110609390224866 1106 14-15 0.09190384084119785 1106 16-17 -0.09376628134443621 1106 18-19 -0.19698131922029916 1106 20-21 -0.007012961697220987 1106 22-23 -0.05303058784127046 1106 24-25 -0.04800096874232196 1106 26-27 0.04886647086362217 1106 28-29 -0.06951015042653808 1106 30-31 -0.08398944475661807 1106 32-33 -0.19222316191351752 1106 34-35 -0.236689848418429 1106 36-37 -0.28797146810772745 1106 38-39 -0.10005821254241454 1106 40-41 -0.22924456707134766 1106 42-43 -0.2449079829623031 1106 44-45 -0.24066334304035308 1106 46-47 -0.14647791428743062 1106 48-49 -0.0010172499527740797 1106 50-51 -0.1206154407237392 1106 52-53 -0.2129947379351691 1106 54-55 -0.12500568611387308 1106 56-57 -0.09504094570618093 1106 58-59 -0.10721389026572581 1106 60-61 -0.3120248194664086 1106 62-63 -0.2117404928501756 1106 64-65 -0.33022400397125296 1106 66-67 -0.3555134642435078 1106 68-69 -0.22194916735847414 1106 70-71 -0.18441052324435958 1106 72-73 -0.14550057417618234 1106 74-75 -0.0024053762169415904 1106 76-77 -0.16100681290465957 1106 78-79 -0.154276099703214 1106 80-81 -0.22636700759536055 1106 82-83 0.0417912435548935 1106 84-85 -0.21719202423682304 1106 86-87 0.198439199418722 1106 88-89 0.43131995571381054 1106 90-91 0.5390913277606408 1106 92-93 0.4075349242393749 1106 94-95 0.430140163210897 1106 96-97 0.46809456875907784 1106 98-99 0.37098155804260813 1106 100-101 0.42988213617090665 1107 1 -0.12830407418470458 1107 2 -0.022383418975763902 1107 3 -0.15173240257142595 1107 4 -0.028467990117121644 1107 5 -0.09221572898743347 1107 6 -0.17003805019646023 1107 7 -0.0022762030010241574 1107 8 -0.05154703470437738 1107 9 -0.06743606467770036 1107 10-11 -0.12594392234081653 1107 12-13 -0.12538344929105705 1107 14-15 -0.1799324814624157 1107 16-17 -0.14624586373055593 1107 18-19 -0.13483178651001992 1107 20-21 -0.10036987000739828 1107 22-23 -0.3644560319769923 1107 24-25 -0.31826281937799905 1107 26-27 -0.282020958621942 1107 28-29 -0.2327037178257072 1107 30-31 -0.3306931578154675 1107 32-33 -0.23711665353597766 1107 34-35 -0.2273524736604955 1107 36-37 -0.19731726250025616 1107 38-39 -0.11827409197324101 1107 40-41 9.945505509492136E-4 1107 42-43 0.038265377313862814 1107 44-45 -0.0010868506601227068 1107 46-47 -0.1887360602838939 1107 48-49 -0.11579370865445782 1107 50-51 -0.00410453729693927 1107 52-53 -0.07486642270404786 1107 54-55 -0.1655083382987712 1107 56-57 -0.08919350948491456 1107 58-59 -0.026473804385453548 1107 60-61 0.030473286115814346 1107 62-63 -0.08665419159564891 1107 64-65 -0.021998020978919897 1107 66-67 -0.21045999956387362 1107 68-69 -0.32891220516263076 1107 70-71 -0.2648432924621744 1107 72-73 -0.17883390750952088 1107 74-75 -0.23038803176969225 1107 76-77 -0.2344260265107856 1107 78-79 -0.2556527099583299 1107 80-81 -0.0954711584752097 1107 82-83 -0.22010990993504365 1107 84-85 -0.19071227345800779 1107 86-87 -0.11965459531058542 1107 88-89 -0.05065781645087952 1107 90-91 0.10745876061405468 1107 92-93 0.2501424850712368 1107 94-95 0.3003725451255228 1107 96-97 0.40248035641589297 1107 98-99 0.23924374546367488 1107 100-101 0.5543832728215783 1108 1 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750.0 10 1268.0 11 1980.0 12 2693.0 13 3712.0 14 4161.0 15 5331.0 16 6914.0 17 9226.0 18 8761.0 19 6176.0 20 5727.0 21 6171.0 22 6521.0 23 7369.0 24 8573.0 25 10677.0 26 12833.0 27 15387.0 28 19215.0 29 23854.0 30 28677.0 31 34797.0 32 43234.0 33 54250.0 34 70945.0 35 98030.0 36 147172.0 37 236989.0 38 215536.0 39 22528.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.334818635947315 20.568244755884002 14.889381998204016 23.207554609964674 2 15.763937306336409 21.859045490132363 38.8934962956747 23.483520907856526 3 18.341208576858836 25.648000271385683 31.14695266908712 24.863838482668363 4 13.156581504530088 17.961447164153522 37.371326138637016 31.51064519267937 5 13.419129298316248 37.81866615722337 34.576499963844995 14.185704580615385 6 30.628373908766203 37.12815028339271 17.973231016169766 14.270244791671317 7 27.165260490083266 33.43498393557447 21.90394910804275 17.49580646629952 8 25.493381849475572 35.24666191146578 20.7532381043353 18.50671813472335 9 26.142386426430825 16.683256306815796 21.174510813916015 35.99984645283737 10-11 24.21836013415737 27.012516771903012 28.212371794814565 20.556751299125047 12-13 25.01783200333519 24.718281128785897 28.43175676701093 21.83213010086799 14-15 22.450351463254815 26.037740565305036 26.65416265687295 24.8577453145672 16-17 21.648843976433607 29.048582137553346 27.344576127668102 21.957997758344945 18-19 21.470736452879844 27.75195092545932 29.59978967543867 21.17752294622217 20-21 22.450648424311513 26.961989042803058 29.40883449669115 21.17852803619428 22-23 22.12511951236278 26.97725448746046 29.191681314649383 21.705944685527374 24-25 21.833513810763836 27.1240616438295 29.46561123822396 21.576813307182704 26-27 21.79092552502957 27.18847501506461 29.424777378534607 21.595822081371214 28-29 21.643052838970984 27.353043313690605 29.425974645078412 21.577929202259998 30-31 21.947201201238734 27.03920898215193 29.354021730494377 21.659568086114962 32-33 21.801197489325347 27.22047497850786 29.170523943983852 21.80780358818294 34-35 22.10000093741672 27.198610480586773 29.173301121909255 21.52808746008725 36-37 22.201944218127434 26.929630911059277 29.107557003984418 21.76086786682887 38-39 21.90146488015124 27.265341288282073 29.144307323507945 21.68888650805874 40-41 21.97692954274677 27.02634296068041 29.28611572011369 21.710611776459135 42-43 21.910811762673568 27.034696217827026 28.98405685378523 22.070435165714176 44-45 21.979959014582366 27.134962186019873 28.864054021119408 22.021024778278353 46-47 22.040709562832628 27.22053073928641 28.53847393372968 22.200285764151285 48-49 21.995462322232257 27.36805315947978 28.411638701579616 22.22484581670835 50-51 21.915165517361434 27.48079643303982 28.29897618172964 22.305061867869103 52-53 22.10878280843117 27.61733190379735 28.086811280717246 22.187074007054242 54-55 21.850787866560374 27.41629224667173 28.165772016386697 22.5671478703812 56-57 21.79909960658003 27.641345965860754 28.046728329540777 22.512826098018436 58-59 21.95440716458842 27.441759152133237 28.07273142791847 22.531102255359873 60-61 22.00497870192198 27.388395437662894 28.204297288406284 22.40232857200884 62-63 21.770666600010536 27.325190572561304 28.311552549284624 22.592590278143543 64-65 21.620154671984874 27.34907072721543 28.146757164269577 22.88401743653012 66-67 21.56433622993817 27.41393655560535 27.95626882540204 23.06545838905444 68-69 21.561845383082993 27.23469727991816 28.070910760300794 23.13254657669806 70-71 21.54839832791371 27.130291945552298 28.04971622294186 23.27159350359213 72-73 21.60078273344302 27.044877729144588 27.88456645690815 23.46977308050424 74-75 21.366667946226357 27.20364728074224 27.746150831520378 23.68353394151103 76-77 21.2746887848154 27.094821554471928 28.012538860011027 23.61795080070165 78-79 21.160683866779127 27.097834886243227 27.951440439836595 23.79004080714105 80-81 21.08648901021892 27.044029648885846 27.813640165795228 24.055841175100007 82-83 21.110949430313244 26.93623418441907 27.95959924190552 23.993217143362163 84-85 20.972578440728565 26.824555405083306 27.79199180129569 24.410874352892446 86-87 20.839852655860238 26.662226076694058 27.993911325292586 24.50400994215312 88-89 20.88680239804137 26.519163327719035 27.943039576101807 24.650994698137787 90-91 20.731420181646456 26.513131096100732 28.036087573466546 24.719361148786263 92-93 20.69753262207669 26.494518277022294 27.93009497606182 24.8778541248392 94-95 20.528139749343186 26.17510447009714 28.029007915713322 25.267747864846356 96-97 20.515373909658923 26.010933986325373 28.068956959480513 25.404735144535195 98-99 20.294230680817428 25.995792623566576 27.934238875961846 25.775737819654154 100-101 20.252900129489795 25.677857423746758 28.39714310072931 25.672099346034134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 394.0 1 270.5 2 184.0 3 245.5 4 222.0 5 215.5 6 360.5 7 641.5 8 926.5 9 1065.0 10 991.5 11 923.0 12 953.5 13 1000.5 14 1105.5 15 1203.0 16 1287.5 17 1373.0 18 1511.5 19 1693.0 20 1881.5 21 2198.0 22 2595.5 23 3087.5 24 3613.5 25 4519.0 26 6131.0 27 7951.0 28 9384.0 29 10994.5 30 13167.0 31 15724.5 32 18366.0 33 20866.5 34 23735.5 35 26257.0 36 29162.5 37 32102.5 38 33570.0 39 33931.5 40 34659.0 41 35847.5 42 36845.5 43 38677.0 44 40649.5 45 41642.5 46 41976.0 47 42540.0 48 43642.0 49 43864.0 50 42577.5 51 40849.0 52 38833.5 53 37326.5 54 36129.5 55 33580.5 56 29975.0 57 26484.0 58 23706.0 59 20982.5 60 17536.5 61 14036.5 62 11237.5 63 8636.5 64 6216.0 65 4493.0 66 3238.5 67 2449.0 68 1777.5 69 1198.0 70 876.0 71 620.5 72 441.0 73 326.5 74 265.0 75 187.5 76 112.5 77 55.0 78 31.0 79 18.5 80 8.0 81 6.0 82 4.5 83 1.5 84 2.0 85 2.0 86 1.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3887778449298637 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.6781481855099685E-4 16-17 0.0020086111391324764 18-19 3.124506216428297E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7854321236733121E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006918549479234086 36-37 0.016916969371804636 38-39 0.010667956938948042 40-41 0.0077219939348870765 42-43 0.0036155000504384575 44-45 0.0020086111391324764 46-47 0.0020086111391324764 48-49 4.4635803091832804E-5 50-51 6.695370463774921E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.927160618366561E-5 60-61 4.90993834010161E-4 62-63 0.0 64-65 0.0 66-67 0.0013390740927549842 68-69 7.588086525611578E-4 70-71 0.0017407963205814795 72-73 0.0 74-75 0.0 76-77 0.09784168037729753 78-79 0.035485463458007085 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1523866317555172 88-89 0.32874268977134863 90-91 0.28316953481458734 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.3390740927549842E-4 100-101 0.0010266234711121544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1120177.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.61376515915302 #Duplication Level Percentage of deduplicated Percentage of total 1 82.7203386697033 55.103132140292196 2 10.64380339813361 14.180476399269343 3 2.9594120316231236 5.914127342511439 4 1.1885024255245524 3.166824858599051 5 0.6517231076681478 2.17068650214997 6 0.3883257869356266 1.5520705665703871 7 0.26222364825170424 1.222739316667076 8 0.17310394415960165 0.9224884387496672 9 0.13758812384110175 0.8248736673215653 >10 0.8052834999298357 9.854209338542121 >50 0.05171690754206129 2.3829323373834645 >100 0.017173453285406554 1.7936471156716114 >500 4.02501700927939E-4 0.1598004365393693 >1k 4.02501700927939E-4 0.7519915397328037 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3526 0.31477168340360495 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2933 0.26183362093669127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1955 0.17452599008906627 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.927160618366562E-5 0.0 14-15 0.0 0.0 0.0 8.927160618366562E-5 0.0 16-17 0.0 0.0 0.0 2.6781481855099685E-4 0.0 18-19 0.0 0.0 0.0 3.570864247346625E-4 0.0 20-21 0.0 0.0 0.0 4.0172222782649527E-4 0.0 22-23 0.0 0.0 0.0 7.588086525611577E-4 0.0 24-25 0.0 0.0 0.0 0.0017854321236733123 0.0 26-27 0.0 0.0 0.0 0.0033476852318874606 0.0 28-29 0.0 0.0 0.0 0.01031087051421338 0.0 30-31 0.0 0.0 0.0 0.028209827554038336 0.0 32-33 0.0 0.0 0.0 0.07007821085417751 0.0 34-35 0.0 0.0 0.0 0.13756754512902872 0.0 36-37 8.927160618366562E-5 0.0 0.0 0.2462557256576416 0.0 38-39 8.927160618366562E-5 0.0 0.0 0.4090871353366477 0.0 40-41 8.927160618366562E-5 0.0 0.0 0.5849968353215608 0.0 42-43 8.927160618366562E-5 0.0 0.0 0.7616653439590351 0.0 44-45 8.927160618366562E-5 0.0 0.0 0.980603958124475 0.0 46-47 8.927160618366562E-5 0.0 0.0 1.2402950605127583 0.0 48-49 8.927160618366562E-5 0.0 0.0 1.5154301507708157 0.0 50-51 8.927160618366562E-5 0.0 0.0 1.8045808831997086 0.0 52-53 8.927160618366562E-5 0.0 0.0 2.1023016898222333 0.0 54-55 8.927160618366562E-5 0.0 0.0 2.4347937870532963 0.0 56-57 8.927160618366562E-5 0.0 0.0 2.8186170578399663 0.0 58-59 8.927160618366562E-5 0.0 0.0 3.2217676313654002 0.0 60-61 8.927160618366562E-5 0.0 0.0 3.6571452547231376 0.0 62-63 8.927160618366562E-5 0.0 0.0 4.099396791757017 0.0 64-65 8.927160618366562E-5 0.0 0.0 4.5656177550512105 0.0 66-67 8.927160618366562E-5 0.0 0.0 5.094596657492522 0.0 68-69 8.927160618366562E-5 0.0 0.0 5.659730560438216 0.0 70-71 8.927160618366562E-5 0.0 0.0 6.269187815854101 0.0 72-73 8.927160618366562E-5 0.0 0.0 6.894356873958312 0.0 74-75 8.927160618366562E-5 0.0 0.0 7.5413528397744285 0.0 76-77 8.927160618366562E-5 0.0 0.0 8.239188985312143 0.0 78-79 8.927160618366562E-5 0.0 0.0 8.97367112518825 0.0 80-81 8.927160618366562E-5 0.0 0.0 9.776312136385588 0.0 82-83 8.927160618366562E-5 0.0 0.0 10.588103487216753 0.0 84-85 8.927160618366562E-5 0.0 0.0 11.41194650488271 0.0 86-87 8.927160618366562E-5 0.0 0.0 12.277211547817888 0.0 88-89 8.927160618366562E-5 0.0 0.0 13.194789751976696 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2910 0.0 42.41376 1 GTATCAA 4585 0.0 33.570915 1 TCAACGC 5615 0.0 27.123848 4 ATCAACG 5620 0.0 27.015295 3 CAACGCA 5715 0.0 26.64924 5 TATCAAC 5815 0.0 26.354137 2 AACGCAG 5860 0.0 26.151764 6 GTACATG 4400 0.0 23.610424 1 GTATAGG 285 3.6379788E-12 23.409016 1 ACGCAGA 6710 0.0 22.626818 7 TACATGG 4560 0.0 22.578285 2 CGCAGAG 6810 0.0 22.015879 8 GAGTACT 3560 0.0 21.057322 12-13 ACATGGG 4580 0.0 21.029387 3 TATATGG 300 1.6916601E-10 20.559765 2 GCAGAGT 7090 0.0 20.47723 9 AGTACTT 3685 0.0 19.313004 12-13 AGAGTAC 6150 0.0 18.978245 10-11 CAGAGTA 6915 0.0 18.62832 10-11 GTACTTT 3990 0.0 18.550165 14-15 >>END_MODULE