FastQCFastQC Report
Thu 26 May 2016
SRR938760_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938760_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116218
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36260.3248469385012605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.25478893907820876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.16439441041086958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.044.1039661
GTATCAA42300.039.9924281
ACGTCGT300.003961373631.65091922-23
CTACCGT600.00397973931.6196544
ATCAACG53350.031.382583
TCAACGC54200.030.8904194
TATCAAC54400.030.6896632
CAACGCA55050.030.3272955
AACGCAG56550.029.6892726
TTTCGCG350.0083731127.15621494-95
GTCCTAT2207.6397555E-1125.9195061
ACGCAGA63700.025.9100747
CGCAGAG64200.025.5605348
GTACATG44550.024.7461971
TACATGG44450.023.9014722
ACATGGG44500.022.9153653
GCAGAGT69450.022.7395239
GAGTACT34300.022.06431612-13
TACACCG1656.395165E-520.1215975
AGAGTAC60100.019.77596510-11
Produced by FastQC (version 0.11.5)