##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938760_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1116218 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46639366145323 33.0 31.0 34.0 30.0 34.0 2 31.542446905532792 33.0 31.0 34.0 28.0 34.0 3 31.553344418384224 33.0 31.0 34.0 28.0 34.0 4 35.11925269078262 37.0 35.0 37.0 32.0 37.0 5 35.1484270993659 37.0 35.0 37.0 32.0 37.0 6 35.19848721307128 37.0 35.0 37.0 32.0 37.0 7 35.10601513324458 37.0 35.0 37.0 32.0 37.0 8 35.078441666412836 37.0 35.0 37.0 32.0 37.0 9 36.639952948259214 39.0 37.0 39.0 32.0 39.0 10-11 36.516421075453 39.0 37.0 39.0 32.0 39.0 12-13 36.41257128983764 39.0 37.0 39.0 32.0 39.0 14-15 37.55813962863885 40.0 37.0 41.0 32.0 41.0 16-17 37.44208210224168 40.0 37.0 41.0 32.0 41.0 18-19 37.38576872976426 40.0 37.0 41.0 32.0 41.0 20-21 37.28998726055305 40.0 37.0 41.0 31.0 41.0 22-23 37.2735702165706 40.0 37.0 41.0 31.0 41.0 24-25 37.207997004169435 40.0 37.0 41.0 31.0 41.0 26-27 37.07524963761559 40.0 37.0 41.0 31.0 41.0 28-29 36.900267689644856 40.0 36.0 41.0 30.0 41.0 30-31 36.68620377023126 39.0 36.0 41.0 30.0 41.0 32-33 36.5102529255038 39.0 35.5 41.0 30.0 41.0 34-35 36.221032540238554 39.0 35.0 41.0 29.5 41.0 36-37 36.026481834193675 39.0 35.0 41.0 29.0 41.0 38-39 35.86709809374155 39.0 35.0 40.5 28.0 41.0 40-41 35.76359277488805 39.0 35.0 40.5 27.0 41.0 42-43 35.77978584828412 39.0 35.0 41.0 27.5 41.0 44-45 35.609991059094185 39.0 35.0 41.0 27.0 41.0 46-47 35.31371112094591 39.0 35.0 41.0 25.5 41.0 48-49 35.04879647165697 39.0 35.0 41.0 24.0 41.0 50-51 33.916570956569416 38.0 33.0 39.5 23.0 40.5 52-53 33.995808166505114 38.0 33.5 39.5 23.0 40.5 54-55 34.50762978199599 38.0 34.0 40.0 23.0 41.0 56-57 34.41334846777242 38.0 34.0 40.0 23.0 41.0 58-59 34.21573518792924 38.0 34.0 40.0 23.0 41.0 60-61 33.91970251330834 38.0 33.5 40.0 22.0 41.0 62-63 33.51946214807502 37.0 33.0 40.0 20.5 41.0 64-65 33.127975001299035 37.0 32.5 40.0 19.5 41.0 66-67 32.738261701567254 36.0 32.0 39.0 18.0 41.0 68-69 32.30219634515838 36.0 31.5 39.0 14.5 40.5 70-71 31.8754969907312 35.0 31.0 38.5 12.0 40.0 72-73 31.432511391144025 35.0 31.0 37.5 8.0 40.0 74-75 30.941041534897302 35.0 30.5 37.0 7.0 39.0 76-77 30.456475348005497 35.0 30.0 36.5 2.0 39.0 78-79 29.99692443590768 34.0 29.5 36.0 2.0 38.5 80-81 29.501878217337474 34.0 29.0 35.5 2.0 37.5 82-83 29.06144050714108 34.0 29.0 35.0 2.0 37.0 84-85 28.69902295071393 34.0 29.0 35.0 2.0 36.5 86-87 28.233222811314636 34.0 28.5 35.0 2.0 36.0 88-89 27.75706582405946 34.0 27.0 35.0 2.0 36.0 90-91 27.278468005353794 34.0 26.0 35.0 2.0 35.0 92-93 26.77278811128292 33.0 25.0 35.0 2.0 35.0 94-95 26.212743836777403 33.0 24.5 35.0 2.0 35.0 96-97 25.560748885970302 33.0 21.5 35.0 2.0 35.0 98-99 24.752196703511324 32.0 10.0 35.0 2.0 35.0 100-101 22.407371140762827 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04803282294600919 1101 2 0.14580810910383946 1101 3 0.36434480656654245 1101 4 0.20409606111402923 1101 5 0.10699926770332979 1101 6 0.18663776015204547 1101 7 0.35343647057226235 1101 8 0.2952537494519589 1101 9 0.48476710561396175 1101 10-11 0.2912295944244505 1101 12-13 0.25526001566131384 1101 14-15 0.26504639483741954 1101 16-17 0.6161390191663401 1101 18-19 0.5079314983981362 1101 20-21 0.611676026259282 1101 22-23 0.40704188717793954 1101 24-25 0.4165079030100145 1101 26-27 0.20248765902530863 1101 28-29 0.5144272263146021 1101 30-31 0.3892221372680851 1101 32-33 0.43693679387367723 1101 34-35 0.6154575223345233 1101 36-37 0.8582307464065195 1101 38-39 0.550131082951161 1101 40-41 0.71721415019924 1101 42-43 1.0079631522005386 1101 44-45 0.7706927134741406 1101 46-47 1.0004071460671042 1101 48-49 0.9660808851095837 1101 50-51 1.0616952764162235 1101 52-53 1.089604239897703 1101 54-55 0.9224972502758249 1101 56-57 0.9597462395306451 1101 58-59 0.885171017291178 1101 60-61 0.7474133757042907 1101 62-63 0.6162817354109862 1101 64-65 0.6044128588959339 1101 66-67 0.5540400435139929 1101 68-69 0.8047623175271141 1101 70-71 0.4224381131261197 1101 72-73 0.4408031700975066 1101 74-75 0.20551886263704233 1101 76-77 -0.05046171671527233 1101 78-79 -0.03818366032387743 1101 80-81 -0.06930640293700208 1101 82-83 -0.14246961490298204 1101 84-85 -0.1940450811884169 1101 86-87 -0.11416083141973132 1101 88-89 -0.43543539714547563 1101 90-91 -0.6454669009812406 1101 92-93 -0.552827729213913 1101 94-95 -0.5726734631973436 1101 96-97 -0.606541872642655 1101 98-99 -0.7671369372829808 1101 100-101 -0.18080846920236482 1102 1 0.2010056717219726 1102 2 0.10708109096978902 1102 3 0.25101283158919685 1102 4 0.36743085395751507 1102 5 0.29757112112226736 1102 6 0.2547616248771334 1102 7 0.25586460414778855 1102 8 0.1698864514387708 1102 9 0.4174912018375849 1102 10-11 0.4926701742979489 1102 12-13 0.3654953725115533 1102 14-15 0.3026922194100763 1102 16-17 0.5373237367944768 1102 18-19 0.635909007491172 1102 20-21 0.47842347175525646 1102 22-23 0.5644374852375194 1102 24-25 0.49152340127377414 1102 26-27 0.48331186319487784 1102 28-29 0.5321932220401138 1102 30-31 0.6091704619382838 1102 32-33 0.487195820799478 1102 34-35 0.5182294355789239 1102 36-37 0.4885219403580834 1102 38-39 0.644934937792037 1102 40-41 0.587304451971022 1102 42-43 0.6930332303638096 1102 44-45 0.7119579787220474 1102 46-47 0.595453337395881 1102 48-49 0.44867687680338264 1102 50-51 0.5771853033376786 1102 52-53 0.33784740873991126 1102 54-55 0.39794626640702546 1102 56-57 0.6899676371055463 1102 58-59 0.4668258416947637 1102 60-61 0.42008007416226434 1102 62-63 0.08681595947902565 1102 64-65 0.16620346109559847 1102 66-67 0.17307798066256908 1102 68-69 0.4901831116451447 1102 70-71 0.44518176100556417 1102 72-73 0.3123943107263649 1102 74-75 0.06648057867417023 1102 76-77 0.3274028609732973 1102 78-79 0.14677110208665312 1102 80-81 0.24610775835765608 1102 82-83 0.08944250235493101 1102 84-85 -0.1334921725002829 1102 86-87 -0.03927538851620227 1102 88-89 0.13320695494906687 1102 90-91 -0.012037037669422546 1102 92-93 -0.07323601634146826 1102 94-95 -0.4411536796094673 1102 96-97 -0.6538863589456554 1102 98-99 -0.6693838603899174 1102 100-101 -0.37008367067979364 1103 1 0.29654846842817406 1103 2 0.5178623186732771 1103 3 0.5106528977337597 1103 4 0.4787954632597291 1103 5 0.5087200051426493 1103 6 0.5259325011246858 1103 7 0.4597333894549607 1103 8 0.5080446443827924 1103 9 0.5864477462039659 1103 10-11 0.6249026371755733 1103 12-13 0.7044522526371253 1103 14-15 0.7764555763304983 1103 16-17 0.7459200115462323 1103 18-19 0.8168173200494095 1103 20-21 0.7209339432451003 1103 22-23 0.8338964092971821 1103 24-25 0.856821429527912 1103 26-27 0.9127583641643824 1103 28-29 0.8784843420488428 1103 30-31 0.9693280169398335 1103 32-33 1.0831566377015847 1103 34-35 1.0883116126415402 1103 36-37 1.2390805597162782 1103 38-39 1.2136795341675182 1103 40-41 1.1233939485840523 1103 42-43 1.3397041779601935 1103 44-45 1.3640669661562583 1103 46-47 1.1675034830314246 1103 48-49 1.4546999770358937 1103 50-51 1.394479419997218 1103 52-53 1.2685467112001803 1103 54-55 1.3492515302717791 1103 56-57 1.3890249931697767 1103 58-59 1.304646176950321 1103 60-61 1.3576972751057497 1103 62-63 1.2527918016050492 1103 64-65 0.9589002789378469 1103 66-67 1.0394873718472155 1103 68-69 0.9599765358013386 1103 70-71 1.0413398364338633 1103 72-73 1.1203723688630696 1103 74-75 0.9487512341600528 1103 76-77 0.9259171265420285 1103 78-79 0.7457688608940352 1103 80-81 0.9162604448509022 1103 82-83 0.6454034871366439 1103 84-85 0.4699099075279207 1103 86-87 0.5769417805301593 1103 88-89 0.445285472236467 1103 90-91 0.5734434757063838 1103 92-93 0.4324345367378619 1103 94-95 0.4858405201504645 1103 96-97 0.36113047098391604 1103 98-99 0.42694209463513744 1103 100-101 0.7105963256268559 1104 1 0.1325695753561078 1104 2 0.19073622717698768 1104 3 0.14827118776955928 1104 4 0.2590600903805509 1104 5 0.3554839718485141 1104 6 0.38486720733060764 1104 7 0.2826977138718689 1104 8 0.28744463845387713 1104 9 0.5608908401739328 1104 10-11 0.4458288979752538 1104 12-13 0.35782168645894075 1104 14-15 0.516458771355488 1104 16-17 0.582762108141381 1104 18-19 0.5636725261285136 1104 20-21 0.6164681547162232 1104 22-23 0.6157091413070503 1104 24-25 0.4530995548058243 1104 26-27 0.5006075523974758 1104 28-29 0.4610971478407393 1104 30-31 0.5274840593249763 1104 32-33 0.44874572455027106 1104 34-35 0.5765097832426989 1104 36-37 0.5903010921984873 1104 38-39 0.5385649908749244 1104 40-41 0.634609474429169 1104 42-43 0.6747371328312894 1104 44-45 0.6409348205816983 1104 46-47 0.7703964628698259 1104 48-49 0.5956639852441441 1104 50-51 0.6685170422398627 1104 52-53 0.7793249797601547 1104 54-55 0.6035170533171339 1104 56-57 0.6062191683427827 1104 58-59 0.6273461706920287 1104 60-61 0.539816993021276 1104 62-63 0.7042224810913424 1104 64-65 0.553670648869037 1104 66-67 0.5243554700168289 1104 68-69 0.6881589618463906 1104 70-71 0.7115062083018344 1104 72-73 0.25524853694114924 1104 74-75 0.37006221549386353 1104 76-77 0.3052538788814836 1104 78-79 0.10001149175007384 1104 80-81 0.24074405305519875 1104 82-83 0.30343946904687513 1104 84-85 0.15352585234951022 1104 86-87 0.13597398440569108 1104 88-89 0.26510335581239275 1104 90-91 0.03870087284798984 1104 92-93 -0.07141019822328687 1104 94-95 -0.04821778740856786 1104 96-97 0.0971052994571906 1104 98-99 0.3776382477591831 1104 100-101 0.5917899803731146 1105 1 0.1603533952904641 1105 2 0.20583097721026533 1105 3 0.22017862265224863 1105 4 0.28215754152835615 1105 5 0.3866750270038466 1105 6 0.26378130734327243 1105 7 0.3739138971840461 1105 8 0.37791249616487477 1105 9 0.3651444462592295 1105 10-11 0.41609553563956325 1105 12-13 0.3864569093820478 1105 14-15 0.48012439804976736 1105 16-17 0.3546226306382323 1105 18-19 0.36263753176125135 1105 20-21 0.22894262885922245 1105 22-23 0.41232528111569877 1105 24-25 0.4295029493795681 1105 26-27 0.3614319767062071 1105 28-29 0.44467781279539764 1105 30-31 0.35006514957530754 1105 32-33 0.5465166557530168 1105 34-35 0.4755596856844164 1105 36-37 0.7057769850759144 1105 38-39 0.7174018234285526 1105 40-41 0.7125400376050521 1105 42-43 0.6777588423900553 1105 44-45 0.5219948781751711 1105 46-47 0.8206446602330004 1105 48-49 0.47562671505802 1105 50-51 0.6625864401055921 1105 52-53 0.9003613701361317 1105 54-55 0.7051180805198243 1105 56-57 0.5168827269962506 1105 58-59 0.5966680675756848 1105 60-61 0.8544197311462725 1105 62-63 0.7568893447142813 1105 64-65 0.7655717854798567 1105 66-67 0.671469627817757 1105 68-69 0.45794738536297785 1105 70-71 0.5874870667498016 1105 72-73 0.5331605948852491 1105 74-75 0.5573148703453192 1105 76-77 0.4565211626056431 1105 78-79 0.31855584424551964 1105 80-81 0.25401425216499973 1105 82-83 -0.008927220237293199 1105 84-85 0.03737711573739588 1105 86-87 0.2224348101286111 1105 88-89 0.14899925901379873 1105 90-91 0.30921489624249787 1105 92-93 0.2968828727036943 1105 94-95 0.3782669327890815 1105 96-97 0.30186325427195726 1105 98-99 0.34834235559598525 1105 100-101 1.022266311532352 1106 1 0.23270559371069766 1106 2 0.18033287437188505 1106 3 0.2543134323564402 1106 4 0.2325791155928627 1106 5 0.2758453830310543 1106 6 0.2780186358389187 1106 7 0.28014460832983445 1106 8 0.2044655524593466 1106 9 0.2517220669585001 1106 10-11 0.36065016480792167 1106 12-13 0.3176804924639427 1106 14-15 0.4877655258817981 1106 16-17 0.5393616808354693 1106 18-19 0.4966874313417122 1106 20-21 0.6430674749400467 1106 22-23 0.5259638522443808 1106 24-25 0.35149999789113906 1106 26-27 0.2818397019608554 1106 28-29 0.19324750019455195 1106 30-31 0.00372897441607023 1106 32-33 0.2136533046543576 1106 34-35 0.24902981518018663 1106 36-37 0.5019073080163778 1106 38-39 0.2773269082603491 1106 40-41 0.472369716043211 1106 42-43 0.5860206600933466 1106 44-45 0.46205569387634426 1106 46-47 0.4740460783260545 1106 48-49 0.3320447924546954 1106 50-51 0.3168299097024416 1106 52-53 0.22362925061025152 1106 54-55 0.23259010874382113 1106 56-57 0.24138149094297745 1106 58-59 0.4285005909729662 1106 60-61 0.4515423432448742 1106 62-63 0.354619017199326 1106 64-65 0.5002581976668026 1106 66-67 0.6595438286129678 1106 68-69 0.5461184024985286 1106 70-71 0.4725999094843445 1106 72-73 0.36124344335357605 1106 74-75 0.2963251190912466 1106 76-77 0.13907479541165557 1106 78-79 0.4562872713309112 1106 80-81 0.41737146059585584 1106 82-83 0.41654378029221917 1106 84-85 0.31238408714054344 1106 86-87 0.47723646195950664 1106 88-89 0.721735276259011 1106 90-91 0.6205185956293278 1106 92-93 0.579892074955854 1106 94-95 0.5050281912135155 1106 96-97 0.5914349751275765 1106 98-99 0.4359470441387572 1106 100-101 0.8913128115746538 1107 1 0.035268081548686325 1107 2 0.08756603410447994 1107 3 0.1673077462811179 1107 4 0.09972169036911538 1107 5 0.14956272180683783 1107 6 0.08357661741241884 1107 7 0.03426590207668312 1107 8 0.115998126729977 1107 9 0.02576913987847007 1107 10-11 -0.013352167233747991 1107 12-13 7.308244198114267E-4 1107 14-15 0.16744382058016072 1107 16-17 0.23450009409715022 1107 18-19 0.04891742703616586 1107 20-21 0.1135913569160536 1107 22-23 0.2647656087835202 1107 24-25 0.020378043756664965 1107 26-27 0.05126904125146581 1107 28-29 -0.07672194730157145 1107 30-31 -0.0500811250710953 1107 32-33 -0.05506738202618777 1107 34-35 0.13647164820783786 1107 36-37 0.062110220157236995 1107 38-39 0.07537355522264733 1107 40-41 0.06375602441511319 1107 42-43 -0.051384016554216316 1107 44-45 0.2038770766452771 1107 46-47 0.3505352064458549 1107 48-49 0.22068482297655834 1107 50-51 0.24528166080219194 1107 52-53 0.02515902091487021 1107 54-55 -0.0658687058986942 1107 56-57 0.14032954402527054 1107 58-59 0.1948628051561272 1107 60-61 0.1362750548025744 1107 62-63 0.039070949101258634 1107 64-65 0.06156239426925936 1107 66-67 -0.055790417924598046 1107 68-69 0.18782555812783386 1107 70-71 0.4193429420884911 1107 72-73 0.29004913770289775 1107 74-75 0.476765694164758 1107 76-77 0.32302032798899205 1107 78-79 0.4338160885935345 1107 80-81 0.22832516144792336 1107 82-83 0.10345875883345101 1107 84-85 0.3151413203768847 1107 86-87 0.30443018230143437 1107 88-89 0.28406529539646286 1107 90-91 0.2301666902348316 1107 92-93 0.3108538352949033 1107 94-95 0.25840329291473907 1107 96-97 0.3189898401618123 1107 98-99 0.33489509722105026 1107 100-101 0.8737995157194653 1108 1 0.03868024167307382 1108 2 -0.004075555880035608 1108 3 -0.02087041321328087 1108 4 0.056487032942285964 1108 5 0.15685366940383716 1108 6 0.09331212264333999 1108 7 0.22119246146652927 1108 8 0.20284191631370874 1108 9 0.30606808297171995 1108 10-11 0.4004009408227347 1108 12-13 0.2737115930061478 1108 14-15 0.3471644641070242 1108 16-17 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2316 60-61 -2.588870625511781 2316 62-63 -2.9554381008783963 2316 64-65 -2.8670686390505153 2316 66-67 -2.707223665959887 2316 68-69 -2.799062209811524 2316 70-71 -2.6797293747705417 2316 72-73 -2.699460808976905 2316 74-75 -2.7981458403434765 2316 76-77 -2.6906091796021805 2316 78-79 -2.4669252281370184 2316 80-81 -2.4460605989537996 2316 82-83 -2.5045976235301985 2316 84-85 -2.402426574545917 2316 86-87 -2.6989749361165174 2316 88-89 -2.5837945624163368 2316 90-91 -2.896642101173974 2316 92-93 -2.743154949689785 2316 94-95 -2.9220345242762065 2316 96-97 -3.1616909560008466 2316 98-99 -3.6873330286488795 2316 100-101 -3.521545539849175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6075.0 3 1349.0 4 1252.0 5 1390.0 6 1578.0 7 1953.0 8 2368.0 9 2809.0 10 3254.0 11 4149.0 12 5001.0 13 6282.0 14 6382.0 15 7201.0 16 8586.0 17 10421.0 18 9390.0 19 7449.0 20 7983.0 21 8222.0 22 9207.0 23 10536.0 24 12385.0 25 13920.0 26 16283.0 27 18716.0 28 21678.0 29 25564.0 30 30224.0 31 36078.0 32 44389.0 33 55545.0 34 71808.0 35 98000.0 36 141037.0 37 202805.0 38 178404.0 39 26543.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5396095326987 19.724590564500406 14.39993028973777 23.335869613063128 2 15.387086555951287 21.09837767601798 39.9618086682478 23.55272709978293 3 18.378356544335784 25.39434767583288 30.894524956128738 25.3327708237026 4 12.71756180029361 17.244498365033614 37.91479868915538 32.123141145517394 5 13.507183908045977 37.42869971264368 34.7116558908046 14.352460488505747 6 30.543769685295572 36.59909899801902 18.421262856871014 14.435868459814397 7 27.259314204431163 33.20318112589442 22.09222966177189 17.445275007902524 8 25.507923179509746 34.6364856915775 21.082707307106837 18.77288382180592 9 26.497335319133665 16.53905507007124 21.313678695113854 35.649930915681246 10-11 24.275758802613023 26.63643814538955 28.429816537233414 20.65798651476401 12-13 25.115888651732547 24.544603720736067 28.46791768505489 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21.900327929592038 27.122155908870376 29.19202878837817 21.785487373159416 40-41 22.04846510971464 26.896594191791955 29.253192509765974 21.801748188727434 42-43 21.85186267611175 26.995146318598433 28.95001764770681 22.202973357583005 44-45 21.97783914335217 27.069458065833242 28.81702791264264 22.135674878171947 46-47 21.96233250484211 27.180792914219005 28.565409059859576 22.291465521079306 48-49 22.067310611597758 27.2006386974171 28.485140164846907 22.24691052613824 50-51 21.90775770915798 27.37319462253539 28.3797489191235 22.339298749183133 52-53 22.040761254545334 27.467994342087803 28.209099883738446 22.282144519628414 54-55 21.900194778532175 27.233330829830305 28.24900350835218 22.61747088328534 56-57 21.846840872109308 27.533329526514123 28.161229884370115 22.458599717006457 58-59 21.9444484400397 27.241858575106804 28.24380403043685 22.56988895441665 60-61 22.02992108788269 27.11582300067359 28.416585519732074 22.437670391711645 62-63 21.905301086820153 27.074633053740243 28.49515722221198 22.524908637227625 64-65 21.798544808429764 27.051718902871023 28.402910383140465 22.746825905558744 66-67 21.726765504096534 27.16415869349975 28.213742202460935 22.89533359994278 68-69 21.68783751441831 26.93803680650403 28.376673738611558 22.997451940466103 70-71 21.699917358755226 26.86551250842945 28.37415384947597 23.060416283339354 72-73 21.758891484884497 26.752364510171017 28.304533975804762 23.184210029139727 74-75 21.534293503758413 27.053181818386278 28.157022455105306 23.255502222750003 76-77 21.507191235597293 26.86041177581694 28.32074080070518 23.311656187880587 78-79 21.445675983842715 26.84129927306604 28.283747037051693 23.42927770603956 80-81 21.53923081177601 26.774682232875293 28.185731495042216 23.500355460306483 82-83 21.527606327363486 26.644496160634983 28.30876814282663 23.5191293691749 84-85 21.44345048038265 26.48405050448723 28.2349153011903 23.83758371393982 86-87 21.429217064550812 26.30802155486232 28.538472227406224 23.724289153180635 88-89 21.577451892201115 26.162061713981437 28.41431801787054 23.84616837594691 90-91 21.395406164279425 26.181139021513534 28.565683609933306 23.857771204273735 92-93 21.521004154588848 26.087498268171604 28.423532354153057 23.967965223086487 94-95 21.42886705162787 25.826574918980004 28.470274073420942 24.27428395597119 96-97 21.563714214892617 25.63283693683035 28.44863459096211 24.354814257314924 98-99 21.41407643498348 25.565209958905683 28.417879965001227 24.602833641109616 100-101 21.718251077111272 25.070473209386833 28.799571853890644 24.41170385961125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1177.0 1 781.0 2 379.5 3 385.5 4 372.5 5 382.0 6 508.0 7 802.0 8 1143.5 9 1286.5 10 1201.5 11 1134.0 12 1174.0 13 1199.0 14 1250.5 15 1376.0 16 1495.0 17 1580.5 18 1699.5 19 1836.5 20 2005.5 21 2316.5 22 2719.5 23 3194.0 24 3743.0 25 4652.5 26 6129.5 27 7931.5 28 9413.5 29 11154.0 30 13361.5 31 15662.5 32 18249.0 33 20686.5 34 23402.5 35 26063.0 36 28983.5 37 31786.5 38 33272.5 39 33884.5 40 34448.5 41 35466.0 42 36559.5 43 38260.5 44 39853.0 45 40714.0 46 41209.0 47 41637.5 48 42803.5 49 42963.5 50 41516.5 51 39754.5 52 37991.0 53 36981.5 54 35597.5 55 32803.5 56 29632.0 57 26461.0 58 23611.0 59 20778.5 60 17623.5 61 14380.0 62 11448.0 63 9002.5 64 6698.5 65 4814.5 66 3548.5 67 2783.0 68 2122.0 69 1490.0 70 1078.0 71 785.0 72 609.0 73 462.0 74 324.0 75 235.5 76 173.5 77 120.5 78 77.0 79 48.5 80 36.0 81 27.5 82 19.0 83 14.5 84 14.0 85 11.5 86 9.0 87 6.0 88 4.5 89 5.0 90 3.5 91 3.5 92 3.5 93 2.0 94 1.5 95 1.0 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2722586448166935 2 0.16385688100353157 3 0.19377935134534652 4 0.16322976336163725 5 0.23454199806847764 6 0.1903749984322059 7 0.23740882157428028 8 0.24305288035132924 9 0.14674552820327214 10-11 0.20237982186275444 12-13 0.20833743946075048 14-15 0.17017285153975298 16-17 0.2849801741236927 18-19 0.22898752752598509 20-21 0.19404811604901553 22-23 0.2442623215178397 24-25 0.2285395863532034 26-27 0.34155514424601646 28-29 0.3142755268236133 30-31 0.28766782116038264 32-33 0.18714982198817792 34-35 0.36063743820651517 36-37 0.3997427025903542 38-39 0.3799884968706829 40-41 0.3412863795423475 42-43 0.374075673389965 44-45 0.3854533791786192 46-47 0.3903807320792175 48-49 0.41035890838527955 50-51 0.4035502025589983 52-53 0.40319184962077304 54-55 0.3979957320165058 56-57 0.37434443809363405 58-59 0.3477815265476815 60-61 0.3157537326937928 62-63 0.3856325556477319 64-65 0.4193177318409128 66-67 0.4237075553341731 68-69 0.4285901141174932 70-71 0.42890367293844034 72-73 0.4190041730199656 74-75 0.41609255539688483 76-77 0.39848846730656556 78-79 0.3717911734087786 80-81 0.3833928497838236 82-83 0.39244126147401315 84-85 0.3938298791096363 86-87 0.3903807320792175 88-89 0.3981301143683402 90-91 0.4197208788964163 92-93 0.4189145847854093 94-95 0.4267983494263665 96-97 0.42773902588920804 98-99 0.42558890825985607 100-101 0.39848846730656556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1116218.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.17839067779201 #Duplication Level Percentage of deduplicated Percentage of total 1 83.77876651230659 57.11901473779557 2 9.966881515942493 13.590518836663826 3 2.7817085766632785 5.689572422745413 4 1.1284469088278064 3.077427768368357 5 0.5852249021453253 1.9949846006418293 6 0.37910637572970746 1.5508117555765084 7 0.2558820275063464 1.2211937387127247 8 0.18049051587299045 0.984444232385997 9 0.128114812371343 0.7861195556518902 >10 0.7534114586047461 9.407244876995932 >50 0.04589613557649876 2.1438261626822603 >100 0.015543366322081238 1.6294553295949226 >500 1.317230327271939E-4 0.05954180488274834 >1k 3.951690981815818E-4 0.7458441773019985 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3626 0.3248469385012605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2844 0.25478893907820876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1835 0.16439441041086958 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.958823455633219E-5 0.0 10-11 0.0 0.0 0.0 8.958823455633219E-5 0.0 12-13 0.0 0.0 0.0 8.958823455633219E-5 0.0 14-15 0.0 0.0 0.0 1.7917646911266437E-4 0.0 16-17 0.0 0.0 0.0 1.7917646911266437E-4 0.0 18-19 0.0 0.0 0.0 1.7917646911266437E-4 0.0 20-21 0.0 0.0 0.0 4.479411727816609E-4 0.0 22-23 0.0 0.0 0.0 7.614999937288235E-4 0.0 24-25 0.0 0.0 0.0 0.001433411752901315 0.0 26-27 0.0 0.0 0.0 0.0030459999749152945 0.0 28-29 0.0 0.0 0.0 0.009227588159302216 0.0 30-31 0.0 0.0 0.0 0.02947452916903329 0.0 32-33 0.0 0.0 0.0 0.06450352888055917 0.0 34-35 0.0 0.0 0.0 0.12833514600194584 0.0 36-37 0.0 0.0 0.0 0.2334221451365235 0.0 38-39 0.0 0.0 0.0 0.3832584674319891 0.0 40-41 0.0 0.0 0.0 0.5533417307371857 0.0 42-43 0.0 0.0 0.0 0.7299201410477165 0.0 44-45 0.0 0.0 0.0 0.9471268157295438 0.0 46-47 0.0 0.0 0.0 1.2031699900915411 0.0 48-49 0.0 0.0 0.0 1.462796693835792 0.0 50-51 0.0 0.0 0.0 1.7405650150777 0.0 52-53 0.0 0.0 0.0 2.0272473656579626 0.0 54-55 0.0 0.0 0.0 2.3467638042031216 0.0 56-57 0.0 0.0 0.0 2.717166359976277 0.0 58-59 0.0 0.0 0.0 3.1136838861226033 0.0 60-61 0.0 0.0 0.0 3.5324192944389 0.0 62-63 0.0 0.0 0.0 3.9678629084999524 0.0 64-65 0.0 0.0 0.0 4.438066757568862 0.0 66-67 0.0 0.0 0.0 4.950914606286585 0.0 68-69 0.0 0.0 0.0 5.505868925245785 0.0 70-71 0.0 0.0 0.0 6.10418394973025 0.0 72-73 0.0 0.0 0.0 6.714369415293429 0.0 74-75 0.0 0.0 0.0 7.336559704287155 0.0 76-77 0.0 0.0 0.0 8.02791211035837 0.0 78-79 0.0 0.0 0.0 8.773510192453447 0.0 80-81 0.0 0.0 0.0 9.56869536237545 0.0 82-83 0.0 0.0 0.0 10.369972532247285 0.0 84-85 0.0 0.0 0.0 11.176445819723387 0.0 86-87 0.0 0.0 0.0 12.02032219512676 0.0 88-89 0.0 0.0 0.0 12.919519305368663 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2435 0.0 44.103966 1 GTATCAA 4230 0.0 39.992428 1 ACGTCGT 30 0.0039613736 31.650919 22-23 CTACCGT 60 0.003979739 31.619654 4 ATCAACG 5335 0.0 31.38258 3 TCAACGC 5420 0.0 30.890419 4 TATCAAC 5440 0.0 30.689663 2 CAACGCA 5505 0.0 30.327295 5 AACGCAG 5655 0.0 29.689272 6 TTTCGCG 35 0.00837311 27.156214 94-95 GTCCTAT 220 7.6397555E-11 25.919506 1 ACGCAGA 6370 0.0 25.910074 7 CGCAGAG 6420 0.0 25.560534 8 GTACATG 4455 0.0 24.746197 1 TACATGG 4445 0.0 23.901472 2 ACATGGG 4450 0.0 22.915365 3 GCAGAGT 6945 0.0 22.739523 9 GAGTACT 3430 0.0 22.064316 12-13 TACACCG 165 6.395165E-5 20.121597 5 AGAGTAC 6010 0.0 19.775965 10-11 >>END_MODULE