FastQCFastQC Report
Thu 26 May 2016
SRR938760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116218
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33670.3016435857511705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29650.26562911545952494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19360.17344282210105913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.047.8574031
GTATCAA42500.037.3924671
ATCAACG53900.029.056353
TATCAAC54750.028.951982
TCAACGC54250.028.8688934
CAACGCA54500.028.6493845
AACGCAG56650.027.7209326
GTATAGG2800.025.4894121
GTACATG47250.023.7649421
ACGCAGA66350.023.5967797
CGCAGAG67900.022.84858
TACATGG47550.022.7562262
ACATGGG47250.022.298063
GAGTACT37100.021.35802812-13
GCAGAGT73200.020.6108449
AGTACTT38700.020.4137112-13
CATGGGG19650.020.0461714
AGAGTAC64300.019.73928510-11
GTACTTT40750.019.50319914-15
CCTATTC3455.2750693E-1119.2586063
Produced by FastQC (version 0.11.5)