##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938760_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1116218 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01660069986329 33.0 31.0 34.0 30.0 34.0 2 32.2197877117194 34.0 31.0 34.0 30.0 34.0 3 32.3019902922189 34.0 31.0 34.0 30.0 34.0 4 35.93998752931775 37.0 35.0 37.0 35.0 37.0 5 35.843271654820114 37.0 35.0 37.0 35.0 37.0 6 35.85558466177754 37.0 35.0 37.0 35.0 37.0 7 35.763645631946446 37.0 35.0 37.0 33.0 37.0 8 35.754914362606584 37.0 35.0 37.0 33.0 37.0 9 37.39247620088549 39.0 37.0 39.0 34.0 39.0 10-11 37.320935516180526 39.0 37.0 39.0 34.0 39.0 12-13 37.24781494295917 39.0 37.0 39.0 33.0 39.0 14-15 38.46182779707907 40.0 38.0 41.0 33.5 41.0 16-17 38.39638672732387 40.0 38.0 41.0 33.0 41.0 18-19 38.52035489483237 40.0 38.0 41.0 34.0 41.0 20-21 38.520064181011236 40.0 38.0 41.0 34.0 41.0 22-23 38.437328102574945 40.0 38.0 41.0 34.0 41.0 24-25 38.36719350521135 40.0 38.0 41.0 34.0 41.0 26-27 38.19383534399195 40.0 38.0 41.0 33.0 41.0 28-29 38.03244885855631 40.0 37.0 41.0 33.0 41.0 30-31 37.81597949504487 40.0 37.0 41.0 33.0 41.0 32-33 37.67415101709523 40.0 37.0 41.0 32.5 41.0 34-35 37.65354930667665 40.0 37.0 41.0 32.5 41.0 36-37 37.550341868703065 40.0 37.0 41.0 32.0 41.0 38-39 37.43395555348507 40.0 37.0 41.0 32.0 41.0 40-41 37.2709847001213 40.0 36.0 41.0 31.0 41.0 42-43 37.08217570402914 40.0 36.0 41.0 31.0 41.0 44-45 36.84498726951187 40.0 36.0 41.0 30.5 41.0 46-47 36.54369979699306 39.0 36.0 41.0 30.0 41.0 48-49 36.25925894404139 39.0 35.0 41.0 30.0 41.0 50-51 36.0390004461494 39.0 35.0 41.0 29.0 41.0 52-53 35.826447880252786 39.0 35.0 40.5 28.5 41.0 54-55 35.564039909766734 38.5 35.0 40.0 28.0 41.0 56-57 35.29526848698014 38.0 34.5 40.0 27.5 41.0 58-59 34.9913780283063 38.0 34.0 40.0 26.5 41.0 60-61 34.64579006968173 38.0 34.0 40.0 26.0 41.0 62-63 34.291271955836585 37.0 33.5 40.0 26.0 41.0 64-65 33.89803828642792 36.5 33.0 39.5 25.5 41.0 66-67 33.49643886767639 36.0 33.0 39.0 24.5 40.5 68-69 33.169225008018145 36.0 32.5 39.0 23.5 40.0 70-71 32.756884407884485 35.0 32.0 38.5 23.0 40.0 72-73 32.281822188855585 35.0 31.5 37.5 22.0 39.5 74-75 31.795328511097296 35.0 31.0 37.0 20.5 39.0 76-77 29.82591303849248 33.0 28.5 35.0 18.0 37.5 78-79 30.79558383756578 34.0 30.5 36.0 18.5 38.0 80-81 30.86497798817077 34.0 31.0 36.0 18.5 37.0 82-83 30.5527029666248 34.0 31.0 35.0 18.0 37.0 84-85 30.16741846126832 34.0 30.0 35.0 14.0 36.5 86-87 29.60363477385242 34.0 29.5 35.0 7.0 36.0 88-89 29.023086888045167 34.0 29.0 35.0 2.0 35.5 90-91 28.52727245036364 34.0 29.0 35.0 2.0 35.0 92-93 28.24748301855014 33.5 29.0 35.0 2.0 35.0 94-95 27.929005803525833 33.0 28.0 35.0 2.0 35.0 96-97 27.349411584475433 33.0 27.0 35.0 2.0 35.0 98-99 26.55602579424449 33.0 25.5 35.0 2.0 35.0 100-101 22.989699592731885 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05233512261601447 1101 2 0.06061483400081613 1101 3 0.10268693994856193 1101 4 0.11300463808490946 1101 5 0.03982828184837217 1101 6 0.029600480132060625 1101 7 0.1241068994091421 1101 8 0.11082466898367471 1101 9 0.18203939020738602 1101 10-11 0.2357971429837633 1101 12-13 0.22218784300609684 1101 14-15 0.35074774201302006 1101 16-17 0.34320259542442244 1101 18-19 0.3540217751695067 1101 20-21 0.2777559482927998 1101 22-23 0.14113805515130196 1101 24-25 0.17728149903135915 1101 26-27 0.16829535832821563 1101 28-29 0.0942012650915629 1101 30-31 0.0423407174610233 1101 32-33 0.22243317648814553 1101 34-35 0.2587866038300959 1101 36-37 0.346617217401608 1101 38-39 0.35323300485700315 1101 40-41 0.1616962449201651 1101 42-43 0.2547435580148374 1101 44-45 0.3278319833429393 1101 46-47 0.3293988360915705 1101 48-49 0.33163966841271275 1101 50-51 0.3028654363393528 1101 52-53 0.2833293631745306 1101 54-55 0.3665458320710471 1101 56-57 0.3596692611409651 1101 58-59 0.41605689201121265 1101 60-61 0.354009821033479 1101 62-63 0.19850870372577134 1101 64-65 0.4197846983765601 1101 66-67 0.47315085198682283 1101 68-69 0.49098718991695733 1101 70-71 0.5593495446528394 1101 72-73 0.5311804076299893 1101 74-75 0.22685306239814196 1101 76-77 0.20246511928208477 1101 78-79 0.10696044488909351 1101 80-81 0.46270061600430523 1101 82-83 0.4717827563879169 1101 84-85 0.5011023707130029 1101 86-87 0.5577271800253385 1101 88-89 0.4198271600854149 1101 90-91 0.28892258611884714 1101 92-93 0.3636195560394633 1101 94-95 0.4957726702049925 1101 96-97 0.10857404293392392 1101 98-99 -0.01892239960223563 1101 100-101 0.635335570560148 1102 1 0.33905950496142623 1102 2 0.21612549637276857 1102 3 0.21892781981642884 1102 4 0.10721370641166317 1102 5 -0.04643830562903162 1102 6 -0.04001902123569323 1102 7 0.12234681686734206 1102 8 0.099326908103464 1102 9 0.2226712858509643 1102 10-11 0.3448988506106474 1102 12-13 0.2443427736215611 1102 14-15 0.3037570062898496 1102 16-17 0.3558721551003359 1102 18-19 0.2650092855197457 1102 20-21 0.4123606450253803 1102 22-23 0.23283618798984662 1102 24-25 0.2132925277259048 1102 26-27 0.25357179099638927 1102 28-29 0.3212176650724885 1102 30-31 0.3617441101718626 1102 32-33 0.46141033443171153 1102 34-35 0.5046285172045515 1102 36-37 0.6355928580326307 1102 38-39 0.5052031268055828 1102 40-41 0.6069277651372715 1102 42-43 0.578208579625695 1102 44-45 0.6592308154458024 1102 46-47 0.7649850172760324 1102 48-49 0.6423016068445051 1102 50-51 0.6211819997565087 1102 52-53 0.3808591783658599 1102 54-55 0.4592969798096007 1102 56-57 0.4782888535474683 1102 58-59 0.50163808774019 1102 60-61 0.38942042748163175 1102 62-63 0.34709912037978796 1102 64-65 0.41838096752179155 1102 66-67 0.5880023070895462 1102 68-69 0.3896099036521079 1102 70-71 0.4485003265023124 1102 72-73 0.5242562602512564 1102 74-75 0.4982751310587048 1102 76-77 0.5195791041349658 1102 78-79 0.476841791048777 1102 80-81 0.579958962307046 1102 82-83 0.6145570973323764 1102 84-85 0.6997315872161636 1102 86-87 0.5726696738802239 1102 88-89 0.423803385965563 1102 90-91 0.49030940977678483 1102 92-93 0.5052745538501959 1102 94-95 0.7073701401947154 1102 96-97 0.7832002421527235 1102 98-99 1.081455627978169 1102 100-101 1.9363105522440165 1103 1 0.16159818022352823 1103 2 0.14351421318507107 1103 3 0.19729065798888712 1103 4 0.08109080175888295 1103 5 0.09886232475885492 1103 6 0.07506328173661814 1103 7 0.06975853318439817 1103 8 0.12267979940981633 1103 9 0.09593226307809744 1103 10-11 0.16240491526108514 1103 12-13 0.19018061871906156 1103 14-15 0.19354153282741038 1103 16-17 0.07257113858937458 1103 18-19 -0.005857728657687744 1103 20-21 0.13562204195149974 1103 22-23 0.20016010327780265 1103 24-25 0.17065866473244284 1103 26-27 0.3240484851113692 1103 28-29 0.18293482152787277 1103 30-31 0.23210077037516186 1103 32-33 0.36755892466280216 1103 34-35 0.35556780354838935 1103 36-37 0.1373669256713228 1103 38-39 0.012140331442417107 1103 40-41 0.14003152312204037 1103 42-43 0.26859453481863227 1103 44-45 0.3143931130459663 1103 46-47 0.48136460983225504 1103 48-49 0.25844406947031473 1103 50-51 0.41522143467378925 1103 52-53 0.3759852139731237 1103 54-55 0.3889512317800907 1103 56-57 0.18932148094710044 1103 58-59 0.433048360292716 1103 60-61 0.19196800036235828 1103 62-63 0.2299800181998748 1103 64-65 0.1629827990881907 1103 66-67 0.11357078937405163 1103 68-69 0.06534542613537297 1103 70-71 -0.004733873929140486 1103 72-73 0.14962118852469075 1103 74-75 0.41828595929269596 1103 76-77 0.26486798295108116 1103 78-79 0.2694353629390136 1103 80-81 0.5701019117836807 1103 82-83 0.47457111527870666 1103 84-85 0.4304757974685707 1103 86-87 0.4720938468633591 1103 88-89 0.3743724977985572 1103 90-91 0.24736622862630497 1103 92-93 0.4050936635846938 1103 94-95 0.6506167637163252 1103 96-97 0.6184444649961023 1103 98-99 0.8103686915289927 1103 100-101 1.094001116571281 1104 1 0.15656519241403544 1104 2 0.16888172340421193 1104 3 0.10598376101691542 1104 4 0.013991983259359131 1104 5 -0.11686938537003044 1104 6 -0.03837986822863115 1104 7 0.03648086575026355 1104 8 -0.013103821640456204 1104 9 0.1483669050541394 1104 10-11 0.09820632304838028 1104 12-13 0.01789466068917278 1104 14-15 0.007481558585247683 1104 16-17 0.3497617893533729 1104 18-19 0.23307496830572205 1104 20-21 0.2469316069340266 1104 22-23 0.039036969650005915 1104 24-25 0.03461318338710839 1104 26-27 0.21028006842741576 1104 28-29 0.09896660049802364 1104 30-31 -0.08246343809857848 1104 32-33 0.10579579788618787 1104 34-35 -0.0722279256584244 1104 36-37 0.07761584117444187 1104 38-39 0.26333764235128854 1104 40-41 0.2965095186226563 1104 42-43 0.16820157968403038 1104 44-45 0.16310703314399433 1104 46-47 0.3679395759880464 1104 48-49 0.26179061110280344 1104 50-51 0.420942835082883 1104 52-53 0.23383707021675804 1104 54-55 0.14791624131575531 1104 56-57 0.0179668562307711 1104 58-59 0.18291907706527866 1104 60-61 0.4849929643771489 1104 62-63 0.4525357417412792 1104 64-65 0.2681534694176122 1104 66-67 0.1681983054562295 1104 68-69 0.17470586118579234 1104 70-71 0.0740967798652008 1104 72-73 0.15443184212140437 1104 74-75 0.1635700036014356 1104 76-77 -0.003068623800633219 1104 78-79 -0.1909059777198152 1104 80-81 -0.13107761238816096 1104 82-83 -0.021122188673569298 1104 84-85 0.11383646967547634 1104 86-87 0.20112443675720115 1104 88-89 0.0749525430559217 1104 90-91 0.1327990556964025 1104 92-93 0.15115917147837976 1104 94-95 0.11068636255953024 1104 96-97 -0.14380203282831872 1104 98-99 -0.025841811209836862 1104 100-101 0.6721590035069234 1105 1 0.18122747193393707 1105 2 0.07718829990386666 1105 3 0.11652687333717893 1105 4 0.07353013163236 1105 5 -0.030579814204429567 1105 6 -0.01285076469348212 1105 7 -0.017862831595046202 1105 8 0.15288323768341883 1105 9 0.026798895080787588 1105 10-11 0.14489388655865554 1105 12-13 0.09942968589849244 1105 14-15 0.17777433083129068 1105 16-17 0.1265619101243587 1105 18-19 0.05340540032172214 1105 20-21 0.08330315302990954 1105 22-23 0.11317233704378538 1105 24-25 -0.01509771294787754 1105 26-27 -0.023039250709921077 1105 28-29 0.05884352447399266 1105 30-31 0.2188009237468833 1105 32-33 0.11754093863957848 1105 34-35 0.2659949552490488 1105 36-37 0.3189922408354988 1105 38-39 0.2905500726726018 1105 40-41 0.2007400743315202 1105 42-43 0.3219435323431057 1105 44-45 0.4964893350000352 1105 46-47 0.3234685821272336 1105 48-49 0.41275515572801424 1105 50-51 0.3350201220211133 1105 52-53 0.26967310087700724 1105 54-55 0.4413126331202122 1105 56-57 0.6655006282787852 1105 58-59 0.6702243257333933 1105 60-61 0.6301827403989151 1105 62-63 0.6171373435801186 1105 64-65 0.34578129349454656 1105 66-67 0.2615385322589461 1105 68-69 0.2968582468997525 1105 70-71 0.20565518010414507 1105 72-73 0.24759350967003968 1105 74-75 0.26933470795600556 1105 76-77 0.4294319199934584 1105 78-79 0.2512351425284791 1105 80-81 0.25097344906544095 1105 82-83 0.3332124183824412 1105 84-85 0.4714038108632508 1105 86-87 0.23566266629349641 1105 88-89 0.18064095391102697 1105 90-91 0.2474357200796753 1105 92-93 0.2635289449678524 1105 94-95 0.3792289294624389 1105 96-97 0.5039119217397143 1105 98-99 0.8613582498249635 1105 100-101 1.806644963879755 1106 1 0.07486265919052215 1106 2 0.09058122698455406 1106 3 0.024592081805636212 1106 4 0.04333533966215697 1106 5 -0.01422286583041199 1106 6 0.05428776413679515 1106 7 -0.07320010651272213 1106 8 0.03973031486390255 1106 9 0.14126933476483572 1106 10-11 0.09694081585140424 1106 12-13 0.0791076540712865 1106 14-15 -0.05139214000944747 1106 16-17 0.035867088388620516 1106 18-19 -0.057291082017911776 1106 20-21 -0.11860983132594782 1106 22-23 -0.09932778045178026 1106 24-25 0.029473835580915875 1106 26-27 -0.002711735756655287 1106 28-29 0.030480096611562146 1106 30-31 -0.02396213314289497 1106 32-33 -0.20579195604367584 1106 34-35 -0.45800327761774895 1106 36-37 -0.4442283594258143 1106 38-39 -0.26321276175892905 1106 40-41 -0.1344990369634047 1106 42-43 -0.28088858060302613 1106 44-45 -0.488341988375943 1106 46-47 -0.2875608042952962 1106 48-49 -0.27584244861588303 1106 50-51 -0.16246860623829207 1106 52-53 -0.26083422927924715 1106 54-55 -0.11061962050727203 1106 56-57 -0.3277895920175524 1106 58-59 -0.20295331815167117 1106 60-61 -0.17538482581374382 1106 62-63 -0.05153849713976655 1106 64-65 -0.11676190570586442 1106 66-67 -0.1010817440279439 1106 68-69 -0.2376948469932998 1106 70-71 -0.2179797564952537 1106 72-73 -0.15105638803048294 1106 74-75 -0.2333676898933561 1106 76-77 -0.07110699296896072 1106 78-79 -0.18154643166135287 1106 80-81 -0.20653624040163265 1106 82-83 -0.08469656799120528 1106 84-85 -0.0871669418289649 1106 86-87 0.06314707071634373 1106 88-89 0.3338394963394542 1106 90-91 0.1323856356395332 1106 92-93 0.3177382625223153 1106 94-95 0.666342643230724 1106 96-97 0.6836558440861182 1106 98-99 0.4280886774147987 1106 100-101 1.1931771529630062 1107 1 0.066753374998612 1107 2 -0.01810616451498248 1107 3 -0.08754280887799837 1107 4 -6.325327098863909E-4 1107 5 -0.02576126254410127 1107 6 -0.030532973566742783 1107 7 0.1322026609864011 1107 8 -0.048330305961748365 1107 9 -0.13017602543753526 1107 10-11 -0.14709347833826314 1107 12-13 0.002134190621681853 1107 14-15 -0.09229863310601871 1107 16-17 -0.044309101803008843 1107 18-19 0.004473930308812157 1107 20-21 -0.07783893986394474 1107 22-23 -0.07566184771938111 1107 24-25 0.02194066680821294 1107 26-27 -0.016320547258125373 1107 28-29 -0.06559710898350346 1107 30-31 -0.11653055067948515 1107 32-33 -0.16283075343338282 1107 34-35 -0.08624794893317755 1107 36-37 -0.24948257801565177 1107 38-39 -0.11985118957672114 1107 40-41 -0.3117736586195221 1107 42-43 -0.18272448883519132 1107 44-45 -0.16653517703688436 1107 46-47 -0.26384737268524816 1107 48-49 -0.37415554895955694 1107 50-51 -0.4876053187337206 1107 52-53 -0.6111032108266912 1107 54-55 -0.5398210111518296 1107 56-57 -0.6417525766018741 1107 58-59 -0.45516086679587886 1107 60-61 -0.5801445964094896 1107 62-63 -0.4125230722623101 1107 64-65 -0.38134674253189615 1107 66-67 -0.6239578163917159 1107 68-69 -0.7880283350810444 1107 70-71 -0.7258538446317147 1107 72-73 -0.6369082344344399 1107 74-75 -0.5108568992209079 1107 76-77 -0.5931602621941359 1107 78-79 -0.681194403689112 1107 80-81 -0.4794686219187483 1107 82-83 -0.509374073595307 1107 84-85 -0.6938467396369639 1107 86-87 -0.7426097166434396 1107 88-89 -0.7030525754849783 1107 90-91 -0.4741727335860979 1107 92-93 -0.1646400369259311 1107 94-95 -0.1952111523126554 1107 96-97 0.0612309344489006 1107 98-99 0.31423156936098096 1107 100-101 0.959798695695909 1108 1 0.12513876059867357 1108 2 0.08187021090472513 1108 3 0.05502818214801408 1108 4 -0.027381924502606125 1108 5 0.06212133837139078 1108 6 0.07857069980416753 1108 7 -0.05282555446977 1108 8 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24.0 6 64.0 7 188.0 8 393.0 9 771.0 10 1300.0 11 1984.0 12 2848.0 13 3636.0 14 4331.0 15 5550.0 16 7244.0 17 9596.0 18 8901.0 19 6150.0 20 6064.0 21 6283.0 22 7115.0 23 7748.0 24 9350.0 25 10999.0 26 13455.0 27 16417.0 28 19829.0 29 24380.0 30 29687.0 31 35849.0 32 43622.0 33 55321.0 34 72949.0 35 100336.0 36 150098.0 37 236638.0 38 199584.0 39 17495.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.34220340763876 20.5606897536453 14.883566804393489 23.213540034322445 2 15.72613951754944 21.821095879120385 38.95224767921678 23.50051692411339 3 18.356270907654242 25.656457788711524 31.08738615575094 24.8998851478833 4 13.174487420915987 17.858608264693814 37.40702980958916 31.559874504801034 5 13.372307670249869 37.79683282733604 34.661113241835636 14.16974626057845 6 30.616029672633445 37.13101829454031 18.033829669049794 14.219122363776451 7 27.110564423795353 33.598992311537714 21.83498205547662 17.45546120919032 8 25.604496612668854 35.21014712179879 20.676874947366912 18.508481318165448 9 26.136561137698912 16.67362468621721 21.155096943428614 36.034717232655275 10-11 24.264391006057956 27.012599689308004 28.210618355912555 20.51239094872149 12-13 25.01446849988085 24.69199564959533 28.40825000134382 21.88528584918 14-15 22.44247987400311 26.010420903443592 26.604435692669355 24.942663529883944 16-17 21.68182919743931 29.003788355588483 27.351327089139016 21.963055357833188 18-19 21.505566117012982 27.754524653786266 29.59130743277747 21.14860179642328 20-21 22.432535579967354 26.91279839601225 29.47148316905837 21.183182854962023 22-23 22.171117111531977 26.968925424961792 29.17817128912094 21.68178617438529 24-25 21.900713349832582 27.14725018757629 29.45971309226514 21.49232337032599 26-27 21.76525369860952 27.222760508566367 29.39079275020124 21.62119304262287 28-29 21.618268116084852 27.32826383376724 29.45495413978273 21.59851391036518 30-31 21.97326149551432 27.020573042183514 29.30991078803603 21.69625467426614 32-33 21.794577761691713 27.172066746818274 29.191743906656225 21.841611584833785 34-35 22.09182727240962 27.212739631891726 29.095704029695224 21.59972906600343 36-37 22.15501390661066 26.95145051683847 29.040981491304784 21.85255408524609 38-39 21.95399389362908 27.23203985493449 29.10243515620975 21.711531095226682 40-41 22.05149179814527 27.067173362708925 29.13464911239343 21.746685726752375 42-43 21.898936394205226 27.109180196557865 28.936421084320617 22.055462324916295 44-45 21.96025958446238 27.200463012529024 28.76465336550888 22.074624037499717 46-47 22.048514912245732 27.291687477350884 28.43645055359033 22.223347056813054 48-49 22.042224908942433 27.35583877110473 28.35840150798767 22.243534811965173 50-51 21.89041198978335 27.50213444160051 28.261305821359105 22.34614774725703 52-53 22.109346023805383 27.612661684366312 28.057736033642172 22.220256258186126 54-55 21.91726884891661 27.38183759803193 28.103157268562235 22.59773628448923 56-57 21.858508521982813 27.653653932556082 27.936353785584412 22.55148375987669 58-59 21.899986830470528 27.432488243731257 28.063505800416227 22.60401912538198 60-61 22.042572672732266 27.4257280711853 28.099496006712144 22.43220324937029 62-63 21.837084273644095 27.362259218479696 28.18307324165994 22.617583266216272 64-65 21.676154281700637 27.29821571915943 28.148203893237007 22.877426105902927 66-67 21.685742021523218 27.3942821868335 27.90340130995701 23.01657448168627 68-69 21.56401628358669 27.252431429042407 28.02862528399521 23.15492700337569 70-71 21.572095044200616 27.107396183987444 28.033962914933692 23.286545856878252 72-73 21.55013626370476 27.095871953328114 27.853878005909237 23.500113777057887 74-75 21.418015981695273 27.1819618899414 27.74753418809736 23.652487940265964 76-77 21.230189321954892 27.133054250779875 27.948608770482984 23.68814765678224 78-79 21.19178175593599 27.090446977594603 27.904161156676317 23.8136101097931 80-81 21.130708809557404 27.027129933860568 27.80382591868411 24.038335337897916 82-83 21.15336547205123 26.914145545209966 27.852012581940738 24.080476400798062 84-85 21.00521582701587 26.764574661938795 27.81450397682173 24.4157055342236 86-87 20.90004990711358 26.717927953498943 27.97026917108006 24.41175296830741 88-89 20.966096102548704 26.544627260312275 27.841814776888423 24.6474618602506 90-91 20.69595529496304 26.533205685169804 27.978555828009537 24.792283191857617 92-93 20.74009968514116 26.4142331066296 27.909689401096294 24.935977807132947 94-95 20.548666207974083 26.15318182143958 28.02109985880869 25.27705211177765 96-97 20.492780304913694 26.053569308848857 27.975954507074114 25.47769587916334 98-99 20.294019626990426 26.01682646221437 27.911841593667187 25.777312317128015 100-101 20.39749937959314 25.530033515408935 28.4099756047959 25.662491500202023 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 391.0 1 272.0 2 194.0 3 238.5 4 221.5 5 224.5 6 332.5 7 590.5 8 887.5 9 1044.0 10 991.0 11 885.0 12 895.5 13 967.0 14 1086.0 15 1194.5 16 1284.5 17 1392.5 18 1479.5 19 1632.5 20 1858.0 21 2149.5 22 2596.0 23 3070.0 24 3532.0 25 4444.5 26 6016.0 27 7781.5 28 9228.0 29 10985.5 30 13241.5 31 15514.0 32 18118.5 33 20732.0 34 23444.0 35 26160.0 36 29075.5 37 31922.0 38 33423.5 39 33964.0 40 34650.5 41 35755.5 42 36778.0 43 38636.5 44 40489.0 45 41463.5 46 41900.0 47 42287.0 48 43527.0 49 43765.0 50 42323.5 51 40632.5 52 38863.5 53 37551.5 54 35881.0 55 33232.0 56 30012.0 57 26443.0 58 23687.5 59 20834.0 60 17434.0 61 14115.5 62 11275.5 63 8616.5 64 6289.0 65 4613.0 66 3304.5 67 2527.0 68 1849.0 69 1243.0 70 881.0 71 623.0 72 434.0 73 317.0 74 257.0 75 185.5 76 108.5 77 60.0 78 34.0 79 25.0 80 19.0 81 9.5 82 5.0 83 5.5 84 3.0 85 2.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23839429215439994 2 0.0 3 0.0 4 0.0 5 0.02418882333020969 6 0.0034043529131406233 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0030907940921934608 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.92735290059827E-4 26-27 0.001926147042961142 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007077470529950243 36-37 0.0180968233803791 38-39 0.015274793991854638 40-41 0.00882444110379872 42-43 0.009765117566640209 44-45 0.004434617610538443 46-47 4.031470555034948E-4 48-49 0.002553264684855467 50-51 8.958823455633219E-5 52-53 0.0 54-55 0.0 56-57 0.001478205870179481 58-59 4.4794117278166087E-4 60-61 0.0029116176230807958 62-63 8.062941110069896E-4 64-65 0.002329294098464637 66-67 0.006674323474446749 68-69 1.7917646911266437E-4 70-71 4.479411727816609E-5 72-73 0.0 74-75 3.1355882094716265E-4 76-77 0.05894905833806657 78-79 0.14119105766077952 80-81 0.001388617635623149 82-83 0.0013438235183449829 84-85 0.0 86-87 0.10271291091883486 88-89 0.3518129971027165 90-91 0.38590132035140084 92-93 3.1355882094716265E-4 94-95 1.7917646911266437E-4 96-97 4.479411727816609E-5 98-99 0.0 100-101 0.0013438235183449829 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1116218.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.42861954554031 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52634661210202 54.821112815787146 2 10.786007145138006 14.32999130119704 3 2.938709570313842 5.856432600036494 4 1.2390365919050335 3.292299614666494 5 0.6573175149248224 2.183234755978052 6 0.38774430403005994 1.5454391312017899 7 0.2619891059433503 1.2182502250650948 8 0.19302864183232382 1.0258100967737445 9 0.13049927595857524 0.7801998078258603 >10 0.807807642724686 9.80666943974795 >50 0.052099928648436995 2.3622325837850737 >100 0.018739582806294293 1.9275371193946016 >500 2.6963346897331534E-4 0.1103340954233833 >1k 4.0445020345997304E-4 0.7404564131173158 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3367 0.3016435857511705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2965 0.26562911545952494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1936 0.17344282210105913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 8.958823455633219E-5 0.0 0.0 8.958823455633219E-5 0.0 16-17 8.958823455633219E-5 0.0 0.0 8.958823455633219E-5 0.0 18-19 8.958823455633219E-5 0.0 0.0 8.958823455633219E-5 0.0 20-21 8.958823455633219E-5 0.0 0.0 3.1355882094716265E-4 0.0 22-23 8.958823455633219E-5 0.0 0.0 5.823235246161592E-4 0.0 24-25 8.958823455633219E-5 0.0 0.0 0.0012542352837886506 0.0 26-27 8.958823455633219E-5 0.0 0.0 0.00286682350580263 0.0 28-29 8.958823455633219E-5 0.0 0.0 0.009317176393858546 0.0 30-31 8.958823455633219E-5 0.0 0.0 0.029474529169033287 0.0 32-33 8.958823455633219E-5 0.0 0.0 0.0651306465224535 0.0 34-35 8.958823455633219E-5 0.0 0.0 0.13088841068680132 0.0 36-37 8.958823455633219E-5 0.0 0.0 0.23875264509262528 0.0 38-39 8.958823455633219E-5 0.0 0.0 0.39199332030123146 0.0 40-41 8.958823455633219E-5 0.0 0.0 0.5642714953530583 0.0 42-43 8.958823455633219E-5 0.0 0.0 0.7435823468175572 0.0 44-45 8.958823455633219E-5 0.0 0.0 0.9629391391287365 0.0 46-47 8.958823455633219E-5 0.0 0.0 1.2213564017064766 0.0 48-49 8.958823455633219E-5 0.0 0.0 1.4836707524874173 0.0 50-51 8.958823455633219E-5 0.0 0.0 1.7652913678152475 0.0 52-53 8.958823455633219E-5 0.0 0.0 2.05493013013587 0.0 54-55 8.958823455633219E-5 0.0 0.0 2.3798666568716866 0.0 56-57 8.958823455633219E-5 0.0 0.0 2.7537183596752604 0.0 58-59 8.958823455633219E-5 0.0 0.0 3.154132974024787 0.0 60-61 8.958823455633219E-5 0.0 0.0 3.577437382303457 0.0 62-63 8.958823455633219E-5 0.0 0.0 4.018838613962505 0.0 64-65 8.958823455633219E-5 0.0 0.0 4.496926227672372 0.0 66-67 8.958823455633219E-5 0.0 0.0 5.015866076339927 0.0 68-69 8.958823455633219E-5 0.0 0.0 5.576733218779844 0.0 70-71 8.958823455633219E-5 0.0 0.0 6.183021596139822 0.0 72-73 8.958823455633219E-5 0.0 0.0 6.802927385152362 0.0 74-75 8.958823455633219E-5 0.0 0.0 7.438511115212261 0.0 76-77 8.958823455633219E-5 0.0 0.0 8.140882874133906 0.0 78-79 8.958823455633219E-5 0.0 0.0 8.897545103196688 0.0 80-81 8.958823455633219E-5 0.0 0.0 9.70348086126545 0.0 82-83 8.958823455633219E-5 0.0 0.0 10.513582472241087 0.0 84-85 8.958823455633219E-5 0.0 0.0 11.328701024351874 0.0 86-87 8.958823455633219E-5 0.0 0.0 12.180864311451707 0.0 88-89 8.958823455633219E-5 0.0 0.0 13.091170362778596 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2575 0.0 47.857403 1 GTATCAA 4250 0.0 37.392467 1 ATCAACG 5390 0.0 29.05635 3 TATCAAC 5475 0.0 28.95198 2 TCAACGC 5425 0.0 28.868893 4 CAACGCA 5450 0.0 28.649384 5 AACGCAG 5665 0.0 27.720932 6 GTATAGG 280 0.0 25.489412 1 GTACATG 4725 0.0 23.764942 1 ACGCAGA 6635 0.0 23.596779 7 CGCAGAG 6790 0.0 22.8485 8 TACATGG 4755 0.0 22.756226 2 ACATGGG 4725 0.0 22.29806 3 GAGTACT 3710 0.0 21.358028 12-13 GCAGAGT 7320 0.0 20.610844 9 AGTACTT 3870 0.0 20.41371 12-13 CATGGGG 1965 0.0 20.046171 4 AGAGTAC 6430 0.0 19.739285 10-11 GTACTTT 4075 0.0 19.503199 14-15 CCTATTC 345 5.2750693E-11 19.258606 3 >>END_MODULE