FastQCFastQC Report
Thu 26 May 2016
SRR938759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996746
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76900.771510495151222No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA69030.6925535693145495No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA55450.5563102334998083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54970.5514945633090076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47740.4789585310600694No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT42060.42197310046892583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.3977944230526132No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31260.3136205211759064No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG27060.27148340700639884No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT25020.25101680869549514No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA23940.24018155076619319No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC21860.21931364660605612No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG16000.16052233969336221No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.1537001402563943No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT14320.14366749402555917No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12600.12641134250852273No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG12400.12440481326235571No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA12260.1230002427900388No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11700.1173819609007711No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG11650.11688032858922935No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11320.11356955533305375No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA11060.11096106731303662No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA11040.11076041438841991No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG10980.11015845561456981No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT10560.10594474419761905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG2700.035.0971763
GACATAT2550.031.603331
ATGGGCG1102.786841E-630.1516655
CATTCCG2200.030.1319899
GTATGGT1354.298763E-728.0918481
TGTTCGC1703.767127E-927.8517028
TACGCCG909.078479E-426.322885
GTTCGCA1658.66894E-825.8274179
GTGTAAG5900.025.7111841
ACGCCGA950.001249585624.919946
CATGGGG13500.024.5680224
GGGCGTA1351.37627485E-524.5507567
TTAGGCA2552.0008883E-1124.1551154
GTACATG57150.023.8061831
TACTCCC2202.2319E-923.6905945
GAGTACT52300.023.67632312-13
AAGCATC5800.022.8736765
CTGTTCG2103.564128E-822.5466147
CCTTTAG2955.456968E-1222.4972861
ACATGGG56100.022.3814873
Produced by FastQC (version 0.11.5)