##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938759_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 996746 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.499229492769473 33.0 31.0 34.0 30.0 34.0 2 31.56726287339001 33.0 31.0 34.0 30.0 34.0 3 31.554263573668717 33.0 31.0 34.0 28.0 34.0 4 35.13135543057108 37.0 35.0 37.0 32.0 37.0 5 35.175265313329575 37.0 35.0 37.0 33.0 37.0 6 35.27692009799889 37.0 35.0 37.0 33.0 37.0 7 35.21162362327012 37.0 35.0 37.0 32.0 37.0 8 35.192600722751834 37.0 35.0 37.0 32.0 37.0 9 36.71937986207118 39.0 37.0 39.0 32.0 39.0 10-11 36.632217234882305 39.0 37.0 39.0 32.0 39.0 12-13 36.556016276965245 39.0 37.0 39.0 32.0 39.0 14-15 37.636697814689 40.0 37.0 41.0 32.0 41.0 16-17 37.57993109578568 40.0 37.0 41.0 32.0 41.0 18-19 37.60827031159393 40.0 37.0 41.0 32.0 41.0 20-21 37.52446962415701 40.0 37.0 41.0 32.0 41.0 22-23 37.43420891581205 40.0 37.0 41.0 32.0 41.0 24-25 37.42259863596142 40.0 37.0 41.0 32.0 41.0 26-27 37.29319405344992 40.0 37.0 41.0 31.5 41.0 28-29 37.17138468576749 40.0 36.0 41.0 31.0 41.0 30-31 36.95684908692887 39.0 36.0 41.0 31.0 41.0 32-33 36.71977314180343 39.0 35.5 41.0 30.0 41.0 34-35 36.40163792982365 39.0 35.0 41.0 30.0 41.0 36-37 36.12197089328676 39.0 35.0 41.0 30.0 41.0 38-39 35.92709727453133 39.0 35.0 41.0 29.5 41.0 40-41 35.739013750744924 39.0 35.0 40.5 28.5 41.0 42-43 35.492020534820305 38.5 35.0 40.0 27.0 41.0 44-45 35.37802108059626 39.0 35.0 41.0 26.5 41.0 46-47 35.13062254576391 39.0 35.0 41.0 24.5 41.0 48-49 34.88072889181396 39.0 35.0 41.0 24.0 41.0 50-51 33.7515515487396 38.0 34.0 40.0 21.5 40.5 52-53 33.85855774690844 38.0 33.5 39.5 22.0 40.5 54-55 34.423404257453754 39.0 34.5 40.0 22.0 41.0 56-57 34.39647011375014 39.0 34.0 41.0 21.5 41.0 58-59 34.22573704835535 39.0 34.0 40.5 19.5 41.0 60-61 33.97357952778341 38.0 34.0 40.0 18.5 41.0 62-63 33.608962564183855 38.0 33.5 40.0 14.0 41.0 64-65 33.186843990344585 37.5 33.0 40.0 10.0 41.0 66-67 32.775631906222856 37.0 32.5 40.0 7.5 41.0 68-69 32.33540390430461 36.5 32.0 39.0 2.0 41.0 70-71 31.88074996037105 36.0 31.5 39.0 2.0 40.5 72-73 31.386654674310204 35.5 31.0 38.5 2.0 40.0 74-75 30.8517801927472 35.0 31.0 37.5 2.0 40.0 76-77 30.32229926179789 35.0 30.0 37.0 2.0 39.0 78-79 29.82466044508832 35.0 29.5 36.5 2.0 39.0 80-81 29.317533253205934 34.5 29.0 36.0 2.0 38.5 82-83 28.78638138502688 34.0 29.0 36.0 2.0 37.0 84-85 28.296438109608665 34.0 28.5 35.0 2.0 37.0 86-87 27.834336430745648 34.0 27.0 35.0 2.0 36.5 88-89 27.364890854841654 34.0 26.0 35.0 2.0 36.0 90-91 26.84728055091267 34.0 25.5 35.0 2.0 36.0 92-93 26.340938413597847 34.0 24.5 35.0 2.0 35.0 94-95 25.78132593459116 33.0 21.5 35.0 2.0 35.0 96-97 25.14871491834429 33.0 14.0 35.0 2.0 35.0 98-99 24.425965592036487 32.5 2.0 35.0 2.0 35.0 100-101 22.381182367423598 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22410082775851592 1101 2 0.03195213069603753 1101 3 0.059334474728551356 1101 4 0.03266729643769395 1101 5 -0.0993707077578776 1101 6 -0.14495236228885489 1101 7 0.08672695483676307 1101 8 0.20495485597268726 1101 9 0.31797427998843375 1101 10-11 0.26908773651877027 1101 12-13 0.21826080524513713 1101 14-15 0.2550067615208249 1101 16-17 0.22183118907739185 1101 18-19 0.23334917432104874 1101 20-21 0.44055383451020447 1101 22-23 0.35011003777990624 1101 24-25 0.27095451321176256 1101 26-27 0.2226729983434339 1101 28-29 0.17026861747813626 1101 30-31 -0.03976591693420062 1101 32-33 0.1313163169081264 1101 34-35 0.15298418907128308 1101 36-37 0.2192225277291655 1101 38-39 0.26654237232001066 1101 40-41 0.42113383002421045 1101 42-43 0.44064880157688435 1101 44-45 0.6239103239800201 1101 46-47 0.5562495417161344 1101 48-49 0.4354160444290187 1101 50-51 0.4509058265591648 1101 52-53 0.19648739756007672 1101 54-55 0.3148567337883392 1101 56-57 0.19979566320819941 1101 58-59 0.3275033215633769 1101 60-61 0.07754717534444211 1101 62-63 -0.0970999871031637 1101 64-65 -0.08472532689859236 1101 66-67 -0.24482865881928007 1101 68-69 -0.43759331226235787 1101 70-71 -0.5299083995416112 1101 72-73 -0.6064343762918902 1101 74-75 -0.45702880134902557 1101 76-77 -0.622901510045125 1101 78-79 -0.44398543251913125 1101 80-81 -0.5908988250631779 1101 82-83 -0.5647070653412136 1101 84-85 -0.7293137157994174 1101 86-87 -0.7223689782292801 1101 88-89 -0.8600520645891372 1101 90-91 -0.9914729623005094 1101 92-93 -0.9359757671913762 1101 94-95 -1.0916159313261922 1101 96-97 -1.0015860771016314 1101 98-99 -1.1291235129307715 1101 100-101 -0.6896853557382698 1102 1 0.29776394351011604 1102 2 0.2482489348724748 1102 3 0.2369048463400425 1102 4 0.11704047762255243 1102 5 0.06042047021704633 1102 6 0.06403721424688058 1102 7 0.24570462640063795 1102 8 0.24183822252236098 1102 9 0.3726004638717413 1102 10-11 0.3462446308202587 1102 12-13 0.19427834964627522 1102 14-15 0.06075895732674752 1102 16-17 0.06090462952607112 1102 18-19 0.2560248387948505 1102 20-21 0.31960703650670297 1102 22-23 0.373600862133074 1102 24-25 0.20540441532643428 1102 26-27 0.31065404739191393 1102 28-29 0.3253187506374502 1102 30-31 0.4436167264986608 1102 32-33 0.4017349971317685 1102 34-35 0.5574104135196905 1102 36-37 0.5727285419445778 1102 38-39 0.5585917250422767 1102 40-41 0.4630492518129543 1102 42-43 0.4216785595973036 1102 44-45 0.33022238415744454 1102 46-47 0.2074311101741273 1102 48-49 0.0760804520905296 1102 50-51 -0.04321444011219455 1102 52-53 0.044834590632262916 1102 54-55 -0.1899829679700744 1102 56-57 -0.026924054070761372 1102 58-59 -0.03846267871881537 1102 60-61 -0.15215870571166334 1102 62-63 -0.10920723326270121 1102 64-65 -0.1254618257786433 1102 66-67 -0.4005875787296844 1102 68-69 -0.4060159255359608 1102 70-71 -0.5500652806153532 1102 72-73 -0.38024542234208525 1102 74-75 -0.6568074570572371 1102 76-77 -0.629302637049129 1102 78-79 -0.9526440949294006 1102 80-81 -0.9051526037894355 1102 82-83 -1.0069712587132322 1102 84-85 -1.3721540877636507 1102 86-87 -1.2201742746189872 1102 88-89 -1.1273506501021124 1102 90-91 -1.193164680849339 1102 92-93 -1.3289280238449308 1102 94-95 -1.2205809483458907 1102 96-97 -1.0899595492969105 1102 98-99 -1.1387907910132746 1102 100-101 -0.9196150061730215 1103 1 0.045712773890766556 1103 2 0.008929087665798363 1103 3 0.023599567291533674 1103 4 0.306549789419968 1103 5 0.15760260709839713 1103 6 0.22688077561399211 1103 7 -0.030748971681376247 1103 8 0.03739098573852573 1103 9 0.20235938463257241 1103 10-11 0.11549159360578898 1103 12-13 0.23746962271307126 1103 14-15 0.19376484523613868 1103 16-17 0.2516743117011302 1103 18-19 0.28905352353754665 1103 20-21 0.43181054132895014 1103 22-23 0.20556365857371617 1103 24-25 0.21062423570048594 1103 26-27 0.24388386783140703 1103 28-29 0.40189593155268 1103 30-31 0.3002764559471558 1103 32-33 0.3736534433694203 1103 34-35 0.44701156710490153 1103 36-37 0.5406057313677266 1103 38-39 0.3588222021178282 1103 40-41 0.7821405634925682 1103 42-43 0.6479305856795037 1103 44-45 0.8192586343946928 1103 46-47 0.8514372882250356 1103 48-49 0.9095981234539963 1103 50-51 1.0442283223840647 1103 52-53 0.9114409384050504 1103 54-55 1.0533874074904972 1103 56-57 1.103034257174734 1103 58-59 0.8836795909731734 1103 60-61 1.0430192732018142 1103 62-63 0.9274498565524567 1103 64-65 0.8204431430584123 1103 66-67 0.5697389068275882 1103 68-69 0.5148027098390529 1103 70-71 0.5912096404683389 1103 72-73 0.799206013858413 1103 74-75 0.7556439769617462 1103 76-77 0.7556584338130286 1103 78-79 0.7109220933309892 1103 80-81 0.44646837104242465 1103 82-83 0.5124813118751455 1103 84-85 0.6362556531377948 1103 86-87 0.42269151638749847 1103 88-89 0.3024994579919671 1103 90-91 0.40535417943988605 1103 92-93 0.40427758177639816 1103 94-95 0.355211432494297 1103 96-97 0.4070790438876024 1103 98-99 0.19758739209001774 1103 100-101 0.09307923048210398 1104 1 0.09748744885734695 1104 2 -4.7483354940425215E-4 1104 3 0.009077938809184616 1104 4 -0.020212064456082146 1104 5 0.02775661039003552 1104 6 -0.020279808813157274 1104 7 0.05076205016490576 1104 8 0.057846794282632175 1104 9 0.010933529160055855 1104 10-11 0.05895326659180711 1104 12-13 0.03112999577997755 1104 14-15 0.10050133588371324 1104 16-17 0.2218333575974185 1104 18-19 0.12221143860291761 1104 20-21 0.28295338866248443 1104 22-23 0.3572124830030958 1104 24-25 0.26043068551368975 1104 26-27 0.29496169775185876 1104 28-29 0.280666887866559 1104 30-31 0.279037970788508 1104 32-33 0.11297009533743108 1104 34-35 0.19736186719352133 1104 36-37 0.13148312311802357 1104 38-39 0.2916348596992222 1104 40-41 0.03190291733749717 1104 42-43 0.3362112506166568 1104 44-45 0.30051784787711 1104 46-47 0.3083069250931203 1104 48-49 0.3588244591541354 1104 50-51 0.462162614026866 1104 52-53 0.5199948052244991 1104 54-55 0.5108058299491844 1104 56-57 0.6356459615965591 1104 58-59 0.33840826665644386 1104 60-61 0.5187439598629382 1104 62-63 0.5070328867930627 1104 64-65 0.12387383084823256 1104 66-67 -0.01077378658848005 1104 68-69 -0.068521503890139 1104 70-71 0.016768994070798016 1104 72-73 -0.04651925216580466 1104 74-75 -0.08145414354148528 1104 76-77 -0.22128184243587512 1104 78-79 -0.34804902220044553 1104 80-81 -0.4094869450430565 1104 82-83 -0.44832584819429044 1104 84-85 -0.31679018735710684 1104 86-87 -0.3422978941427459 1104 88-89 -0.431449502265874 1104 90-91 -0.31113395756446494 1104 92-93 -0.5525207076004435 1104 94-95 -0.5503652590849839 1104 96-97 -0.5340737599078729 1104 98-99 -0.528610441092038 1104 100-101 -0.3798087228426894 1105 1 0.14065415132690262 1105 2 0.17217934001458346 1105 3 0.10660290606007194 1105 4 0.1964480734482308 1105 5 0.17802344722573338 1105 6 0.08286726524828225 1105 7 0.1560667748828024 1105 8 0.17635752040736463 1105 9 0.19263425069360096 1105 10-11 0.14070706064515548 1105 12-13 0.21827170361997617 1105 14-15 0.40573782450471185 1105 16-17 0.256313647053247 1105 18-19 0.3034082871061443 1105 20-21 0.30983872674706703 1105 22-23 0.476023321591029 1105 24-25 0.2989851332857043 1105 26-27 0.4857990009939144 1105 28-29 0.44868721969617553 1105 30-31 0.34502954236690897 1105 32-33 0.2519242718498873 1105 34-35 0.11708494236695088 1105 36-37 0.07722542233751994 1105 38-39 0.015511386060680366 1105 40-41 0.10376879549816209 1105 42-43 -0.07520397445715332 1105 44-45 -0.09356456616436759 1105 46-47 -0.08808635491496375 1105 48-49 -0.03759304045623679 1105 50-51 -0.03419391536731098 1105 52-53 -0.03830283052110417 1105 54-55 -0.11707419667131802 1105 56-57 -0.3349461760887209 1105 58-59 -0.3321077372355319 1105 60-61 -0.1351389688977278 1105 62-63 -0.1323082768429984 1105 64-65 -0.3524351860044064 1105 66-67 -0.3406384516629757 1105 68-69 -0.30895107539272004 1105 70-71 -0.1818317447323743 1105 72-73 -0.24887613537677566 1105 74-75 -0.3340951332176161 1105 76-77 -0.26095776309666974 1105 78-79 -0.2535646680635253 1105 80-81 0.021668308925796964 1105 82-83 -0.08848731922975261 1105 84-85 -0.32045682453904334 1105 86-87 -0.1659023185375723 1105 88-89 0.042424918043213466 1105 90-91 0.3906305984165144 1105 92-93 0.2266628601365177 1105 94-95 -0.35584920885984417 1105 96-97 -0.17254670345303325 1105 98-99 -0.15112951139628095 1105 100-101 0.2276199556936902 1106 1 0.02390314583004738 1106 2 0.06480202334558527 1106 3 0.11036848649346354 1106 4 0.14221057699766249 1106 5 0.24018836990101278 1106 6 0.1398754406052376 1106 7 0.0967223291669157 1106 8 0.191143304016812 1106 9 0.26668607457377647 1106 10-11 0.3088581331045006 1106 12-13 0.3338921873603766 1106 14-15 0.22607068740537528 1106 16-17 0.30352008223314186 1106 18-19 0.2909529464238929 1106 20-21 0.25664963824215903 1106 22-23 0.14740914842942487 1106 24-25 0.18800010660665833 1106 26-27 0.07157614728999562 1106 28-29 0.25829707843922023 1106 30-31 0.37159468457743117 1106 32-33 0.4423997055167632 1106 34-35 0.4348129432823882 1106 36-37 0.40904627225254586 1106 38-39 0.4490288662368158 1106 40-41 0.6550999928128789 1106 42-43 0.46744223050738753 1106 44-45 0.4088003115867096 1106 46-47 0.7307032981091552 1106 48-49 0.843292665595385 1106 50-51 0.6158698685218553 1106 52-53 0.7606762034390471 1106 54-55 0.6056984529665428 1106 56-57 0.5976452722081902 1106 58-59 0.25221890832523286 1106 60-61 0.5438839721117077 1106 62-63 0.45932172998039533 1106 64-65 0.49136421472370984 1106 66-67 0.4327337877236843 1106 68-69 0.35893776263858257 1106 70-71 0.3461738326495585 1106 72-73 0.24407902942299486 1106 74-75 0.2926133638135724 1106 76-77 -0.02918914816064344 1106 78-79 0.1548413492365306 1106 80-81 0.11419944860192288 1106 82-83 0.3308869841272717 1106 84-85 0.20166834445647908 1106 86-87 0.25445576124950264 1106 88-89 0.056220038257830396 1106 90-91 0.24719578300836176 1106 92-93 0.4816087603793626 1106 94-95 0.512748562730259 1106 96-97 0.4767245497202701 1106 98-99 0.5824026962891473 1106 100-101 0.7508862417760866 1107 1 2.6251124737441955E-4 1107 2 0.11583983173999357 1107 3 0.09578099573753462 1107 4 0.10314088588386028 1107 5 0.10188965823301999 1107 6 0.08135085268317965 1107 7 -0.04376550547382152 1107 8 0.22998472041409457 1107 9 0.10955943260005085 1107 10-11 0.02638342478594069 1107 12-13 0.007983217426371425 1107 14-15 0.0800968958675341 1107 16-17 -0.029551664405062184 1107 18-19 0.15137452879897495 1107 20-21 0.1985274239563708 1107 22-23 -0.12653997260772343 1107 24-25 -0.020866525205157416 1107 26-27 0.20946880347145935 1107 28-29 -0.10587683533605485 1107 30-31 0.06559821284194811 1107 32-33 0.20936909040581497 1107 34-35 0.2579457981154505 1107 36-37 0.34853664105822446 1107 38-39 0.30446101552217186 1107 40-41 0.11664096286470738 1107 42-43 0.044442343066812384 1107 44-45 0.0743883714514979 1107 46-47 0.2604189856315742 1107 48-49 0.17141600547540037 1107 50-51 0.060307066149896116 1107 52-53 0.14181923911407068 1107 54-55 0.1738495013996939 1107 56-57 -0.08808619046468635 1107 58-59 -0.08815289543425564 1107 60-61 0.13266600552397279 1107 62-63 0.08954141020644357 1107 64-65 0.13244272334291907 1107 66-67 0.12196511206418137 1107 68-69 -0.05373190491138047 1107 70-71 0.0052429136281766375 1107 72-73 -0.28883145217353956 1107 74-75 -0.2046136769571305 1107 76-77 -0.17804793237160865 1107 78-79 -0.001711450074228793 1107 80-81 -0.004822147027830681 1107 82-83 -0.4372995152641721 1107 84-85 -0.2917385136734154 1107 86-87 -0.09151403285090964 1107 88-89 -0.0696950330280508 1107 90-91 0.002915011418782143 1107 92-93 0.032212110442323194 1107 94-95 -0.30258981508858795 1107 96-97 -0.13689471961128774 1107 98-99 0.07326434392766501 1107 100-101 0.4036761901144672 1108 1 0.09076763303454172 1108 2 0.05282566074377115 1108 3 -0.012974909679559943 1108 4 0.04849579230436518 1108 5 0.06416615955970428 1108 6 -0.09052046661180668 1108 7 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2316 92-93 -2.494426654385517 2316 94-95 -2.7868282542312386 2316 96-97 -3.012589080265272 2316 98-99 -3.0087390621352768 2316 100-101 -3.445098023281176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4839.0 3 1052.0 4 912.0 5 976.0 6 1099.0 7 1405.0 8 1666.0 9 2044.0 10 2633.0 11 3678.0 12 5080.0 13 6928.0 14 7536.0 15 8052.0 16 9940.0 17 12639.0 18 10445.0 19 7326.0 20 7501.0 21 7816.0 22 8616.0 23 9703.0 24 11015.0 25 12652.0 26 14409.0 27 16283.0 28 18682.0 29 21775.0 30 25341.0 31 30121.0 32 36400.0 33 44828.0 34 58021.0 35 81806.0 36 109281.0 37 171167.0 38 188329.0 39 34744.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.74724996457888 23.028751900430983 15.476567296609922 20.747430838380215 2 14.080406965243073 23.653649763671364 41.068364095720376 21.197579175365185 3 17.505090790789584 29.95925359166837 32.21064828760659 20.325007329935456 4 11.212092606614702 19.18170844622618 41.69416599215491 27.912032955004207 5 10.81580871717399 40.35771109896821 36.4283332261901 12.398146957667706 6 25.34650782339894 41.03304997139617 19.514638136435263 14.105804068769634 7 23.523047307049186 36.37987759223238 24.641237647313872 15.455837453404552 8 22.50430841096168 35.63931599396572 23.076923076923077 18.779452518149526 9 25.214998072846406 18.643203571657995 24.303715391533373 31.83808296396223 10-11 21.29082702420037 28.61838770579751 30.431583839117867 19.659201430884256 12-13 23.9318143835998 27.557901615057208 29.762702371470684 18.747581629872307 14-15 21.789137700870228 28.97148420640577 28.97248793022112 20.266890162502886 16-17 17.968368517064086 31.888128031475908 30.7666983667355 19.376805084724495 18-19 17.722840998457755 31.238918111588497 33.294722900720835 17.74351798923291 20-21 18.656451616950694 30.398823579600347 32.5199436884092 18.424781115039764 22-23 18.830814205631498 29.80990089467851 31.85314502786835 19.50613987182164 24-25 18.683066956638733 30.619765090495783 32.41738105422116 18.27978689864432 26-27 18.855993895153276 29.87172793268603 33.07682266825982 18.195455503900877 28-29 18.03523264458682 29.405398296146778 34.13228330487888 18.427085754387516 30-31 18.345164675925947 30.659029958736276 33.110409188945276 17.885396176392494 32-33 17.856485034150513 31.001810285856536 32.66695206983377 18.474752610159182 34-35 18.527019419004635 30.00248039207301 32.60578520005897 18.864714988863394 36-37 19.229158340015655 29.61436748616873 32.36116690212293 18.79530727169268 38-39 18.815088190135747 29.855194130164538 32.91523034045026 18.41448733924946 40-41 17.907764884639356 29.756079375339112 34.090246147382125 18.245909592639407 42-43 17.835558438037474 30.25895471731772 32.794430158017455 19.11105668662735 44-45 17.87016673151006 30.67574541342198 32.17307339532765 19.281014459740316 46-47 18.395174031694356 30.221820170188963 31.309678898735804 20.073326899380877 48-49 18.40201417889195 30.142217360951847 31.62160050606497 19.83416795409124 50-51 18.719221753564366 30.237839521448194 31.59640410794934 19.446534617038104 52-53 17.980902265871308 30.57865277557264 32.390946595396684 19.049498363159366 54-55 18.072362021782187 30.084417318905952 32.080445925037424 19.762774734274437 56-57 18.338194212094216 31.068926387416408 30.829073765766786 19.763805634722598 58-59 17.879939584893 29.756354619347547 31.726797969101238 20.636907826658216 60-61 18.396416941063656 29.8212197428844 31.44559284355191 20.33677047250003 62-63 19.129000609227916 29.335357038295694 31.601896517927376 19.93374583454902 64-65 18.890189995770704 29.071102102500223 32.03050135070353 20.00820655102554 66-67 17.969575849412703 29.611448602724234 31.51692776268013 20.902047785182933 68-69 17.801237695516818 29.763426223516216 31.72115299781954 20.71418308314743 70-71 18.192258441807237 29.426289839582974 31.391726071043262 20.98972564756652 72-73 18.112446661306542 28.74298374067754 31.055936001129155 22.08863359688676 74-75 18.082990404719613 29.30633320765445 30.966000165331582 21.64467622229436 76-77 18.508050900844147 28.518835832178407 31.80214186720423 21.170971399773215 78-79 18.06538146240397 28.29387546978346 32.09129280074329 21.549450267069282 80-81 18.061985661507578 29.117832013496 31.255152202875358 21.565030122121065 82-83 18.19288176710608 28.813891575123325 31.087529360304416 21.90569729746618 84-85 18.08531224110928 27.806652597121186 31.205919287573042 22.902115874196493 86-87 18.32862117798844 27.83764986921879 31.683272604486373 22.150456348306395 88-89 19.24637856473221 26.895696615961533 31.81212890999083 22.04579590931543 90-91 18.599640010544636 26.91962033259961 32.387810763089945 22.092928893765812 92-93 18.7588273187432 27.48407428204474 31.671890389526077 22.08520800968598 94-95 18.522609042810593 26.849019663193214 31.65240789431235 22.97596339968384 96-97 18.789352837840223 26.183993971212033 31.043217235478103 23.983435955469638 98-99 18.9727964192285 26.029597818471416 31.23179749285509 23.765808269444992 100-101 19.58766783406924 25.59428921915736 31.74716771739618 23.07087522937722 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4395.0 1 2665.5 2 1052.0 3 1201.0 4 992.0 5 982.0 6 1702.0 7 2844.0 8 3952.5 9 4122.5 10 3276.5 11 2647.5 12 2556.5 13 2489.0 14 2411.0 15 2328.0 16 2367.5 17 2394.0 18 2363.5 19 2489.0 20 2661.0 21 2954.0 22 3349.5 23 3744.0 24 4248.0 25 5256.5 26 7325.0 27 9931.5 28 11152.5 29 12579.5 30 15770.0 31 18887.0 32 23934.5 33 30701.5 34 36864.0 35 41909.5 36 48552.0 37 54331.5 38 53174.0 39 49617.0 40 45836.0 41 41521.0 42 37745.5 43 36541.0 44 36542.5 45 35462.0 46 33742.0 47 32825.0 48 44981.5 49 43980.0 50 26482.5 51 18603.5 52 13982.0 53 11249.5 54 9143.0 55 7580.0 56 6520.5 57 5617.0 58 4837.0 59 4160.0 60 3411.5 61 2757.0 62 2198.5 63 1636.5 64 1237.5 65 964.0 66 729.0 67 561.0 68 400.5 69 300.0 70 261.0 71 197.0 72 151.5 73 136.5 74 110.5 75 87.0 76 68.5 77 52.5 78 41.5 79 30.5 80 19.0 81 17.0 82 15.0 83 7.0 84 4.0 85 6.5 86 5.0 87 2.0 88 2.5 89 2.5 90 1.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15821483106027012 2 0.08898957206750767 3 0.08327096371593165 4 0.09671470966525073 5 0.11978979599617154 6 0.03772274982794012 7 0.04053189077257396 8 0.04404331695336625 9 0.04594951973722493 10-11 0.04404331695336625 12-13 0.046701968204537564 14-15 0.04574886681260823 16-17 0.04298988909912856 18-19 0.04710327405377097 20-21 0.050514373772254914 22-23 0.06360697710349478 24-25 0.07905725229898088 26-27 0.08186639324361472 28-29 0.07118162500777529 30-31 0.05944342891769819 32-33 0.04961143561147976 34-35 0.2961135535030991 36-37 0.4715845360804056 38-39 0.45011467314641845 40-41 0.42854448375012294 42-43 0.4560339344226112 44-45 0.46270564416611654 46-47 0.4628561338595791 48-49 0.4794100001404571 50-51 0.4684744157488468 52-53 0.47589857395966473 54-55 0.46661837619614227 56-57 0.44845928651833067 58-59 0.42929693221743553 60-61 0.3975937701279965 62-63 0.45212120239258546 64-65 0.4871351377382001 66-67 0.48853970821051707 68-69 0.4999769249136691 70-71 0.4972179472001894 72-73 0.4869344848135834 74-75 0.4815670190800866 76-77 0.4636085823268917 78-79 0.4414865973879002 80-81 0.44665341019678034 82-83 0.4562345873472279 84-85 0.45467952718144844 86-87 0.4646118469499752 88-89 0.4751461254923521 90-91 0.47880804136660693 92-93 0.4812660396931616 94-95 0.4857807304970373 96-97 0.4854295878789582 98-99 0.4796608162962279 100-101 0.4672704982011465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 996746.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.22501779348463 #Duplication Level Percentage of deduplicated Percentage of total 1 79.50513384151758 27.210646203993154 2 9.998656050176757 6.844083624564645 3 3.375493211023649 3.465789456272128 4 1.7428881479827025 2.3860151150704585 5 0.9916715098289378 1.6969987534593582 6 0.6442017918906564 1.322869067403144 7 0.4481771081956915 1.073720865184102 8 0.3428421558389272 0.9387023107155131 9 0.2546081690226304 0.784257220364946 >10 1.9978690710602234 14.364695107694542 >50 0.3639030401885186 8.85782278687685 >100 0.31107829602645787 20.614947385195425 >500 0.016140854982498034 3.648188607451044 >1k 0.006162871902408341 4.216479206563372 >5k 0.0011738803623634934 2.5747842891913306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7690 0.771510495151222 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6903 0.6925535693145495 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5545 0.5563102334998083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5497 0.5514945633090076 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4774 0.4789585310600694 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4206 0.42197310046892583 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3965 0.3977944230526132 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3126 0.3136205211759064 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2706 0.27148340700639884 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2502 0.25101680869549514 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2394 0.24018155076619319 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2186 0.21931364660605612 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1600 0.16052233969336221 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1532 0.1537001402563943 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1432 0.14366749402555917 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1260 0.12641134250852273 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1240 0.12440481326235571 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1226 0.1230002427900388 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1170 0.1173819609007711 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1165 0.11688032858922935 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1132 0.11356955533305375 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1106 0.11096106731303662 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1104 0.11076041438841991 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1098 0.11015845561456981 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1056 0.10594474419761905 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5048969346252705E-4 0.0 14-15 0.0 0.0 0.0 2.5081615577087846E-4 0.0 16-17 0.0 0.0 0.0 4.013058492334055E-4 0.0 18-19 0.0 0.0 0.0 6.019587738501082E-4 0.0 20-21 0.0 0.0 0.0 0.0010032646230835138 0.0 22-23 0.0 0.0 0.0 0.003310773256175596 0.0 24-25 0.0 0.0 0.0 0.008929055145443272 0.0 26-27 0.0 0.0 0.0 0.01640337658741545 0.0 28-29 0.0 0.0 0.0 0.04268890971220351 0.0 30-31 0.0 0.0 0.0 0.11106139377534496 0.0 32-33 0.0 0.0 0.0 0.2374727362838677 0.0 34-35 0.0 0.0 0.0 0.46661837619614227 0.0 36-37 0.0 0.0 0.0 0.8037654527833571 0.0 38-39 0.0 0.0 0.0 1.1976973070370986 0.0 40-41 0.0 0.0 0.0 1.6092364554259562 0.0 42-43 0.0 0.0 0.0 2.034720982075674 0.0 44-45 0.0 0.0 0.0 2.513679513135744 0.0 46-47 0.0 0.0 0.0 3.100539154408445 0.0 48-49 0.0 0.0 0.0 3.6927662614146435 0.0 50-51 0.0 0.0 0.0 4.260965180697991 0.0 52-53 0.0 0.0 0.0 4.791341023691091 5.016323115417569E-5 54-55 0.0 0.0 0.0 5.3670644276475645 1.0032646230835138E-4 56-57 0.0 0.0 0.0 6.029821037656534 1.0032646230835138E-4 58-59 0.0 0.0 0.0 6.7491617724074136 1.0032646230835138E-4 60-61 0.0 0.0 0.0 7.53381503412103 1.0032646230835138E-4 62-63 0.0 0.0 0.0 8.277585262443992 1.0032646230835138E-4 64-65 0.0 0.0 0.0 9.019248635058482 1.0032646230835138E-4 66-67 0.0 0.0 0.0 9.84995174297163 1.0032646230835138E-4 68-69 0.0 0.0 0.0 10.737289138857843 1.0032646230835138E-4 70-71 0.0 0.0 0.0 11.712311862801556 1.0032646230835138E-4 72-73 0.0 0.0 0.0 12.666767661972058 1.0032646230835138E-4 74-75 0.0 0.0 0.0 13.65704000818664 1.0032646230835138E-4 76-77 0.0 0.0 0.0 14.665471444079033 1.0032646230835138E-4 78-79 0.0 0.0 0.0 15.766704857606653 1.0032646230835138E-4 80-81 0.0 0.0 0.0 16.919154930142682 1.0032646230835138E-4 82-83 0.0 0.0 0.0 18.043714246156995 1.0032646230835138E-4 84-85 0.0 0.0 0.0 19.25661101223381 1.0032646230835138E-4 86-87 0.0 1.0032646230835138E-4 0.0 20.534519325886436 1.0032646230835138E-4 88-89 0.0 1.0032646230835138E-4 0.0 21.765123712560673 1.0032646230835138E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 270 0.0 35.097176 3 GACATAT 255 0.0 31.60333 1 ATGGGCG 110 2.786841E-6 30.151665 5 CATTCCG 220 0.0 30.131989 9 GTATGGT 135 4.298763E-7 28.091848 1 TGTTCGC 170 3.767127E-9 27.851702 8 TACGCCG 90 9.078479E-4 26.32288 5 GTTCGCA 165 8.66894E-8 25.827417 9 GTGTAAG 590 0.0 25.711184 1 ACGCCGA 95 0.0012495856 24.91994 6 CATGGGG 1350 0.0 24.568022 4 GGGCGTA 135 1.37627485E-5 24.550756 7 TTAGGCA 255 2.0008883E-11 24.155115 4 GTACATG 5715 0.0 23.806183 1 TACTCCC 220 2.2319E-9 23.690594 5 GAGTACT 5230 0.0 23.676323 12-13 AAGCATC 580 0.0 22.873676 5 CTGTTCG 210 3.564128E-8 22.546614 7 CCTTTAG 295 5.456968E-12 22.497286 1 ACATGGG 5610 0.0 22.381487 3 >>END_MODULE