##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938759_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 996746 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88871588147833 31.0 31.0 34.0 30.0 34.0 2 32.11895708635901 33.0 31.0 34.0 30.0 34.0 3 32.18789541166957 34.0 31.0 34.0 30.0 34.0 4 35.854667086700125 37.0 35.0 37.0 35.0 37.0 5 35.76286235410024 37.0 35.0 37.0 33.0 37.0 6 35.79646770591505 37.0 35.0 37.0 33.0 37.0 7 35.734360609423064 37.0 35.0 37.0 33.0 37.0 8 35.71934976413249 37.0 35.0 37.0 33.0 37.0 9 37.32719970784934 39.0 37.0 39.0 34.0 39.0 10-11 37.26159673577823 39.0 37.0 39.0 33.0 39.0 12-13 37.21310945817691 39.0 37.0 39.0 33.0 39.0 14-15 38.34933674175768 40.0 38.0 41.0 33.0 41.0 16-17 38.284005654399415 40.0 38.0 41.0 33.0 41.0 18-19 38.36522845338732 40.0 38.0 41.0 33.5 41.0 20-21 38.44701458546109 40.0 38.0 41.0 34.0 41.0 22-23 38.388217760592966 40.0 38.0 41.0 34.0 41.0 24-25 38.304877069985736 40.0 38.0 41.0 33.0 41.0 26-27 38.09835404405937 40.0 37.0 41.0 33.0 41.0 28-29 37.931403787925916 40.0 37.0 41.0 33.0 41.0 30-31 37.689198150782644 40.0 37.0 41.0 33.0 41.0 32-33 37.4381532506777 40.0 36.0 41.0 32.0 41.0 34-35 37.35030790191283 40.0 36.0 41.0 32.0 41.0 36-37 37.216518049733835 40.0 36.0 41.0 31.5 41.0 38-39 37.074739201361226 40.0 35.5 41.0 31.0 41.0 40-41 36.90551002963643 40.0 35.0 41.0 31.0 41.0 42-43 36.735608670614184 40.0 35.0 41.0 31.0 41.0 44-45 36.454776843849885 39.5 35.0 41.0 30.0 41.0 46-47 36.10050955810206 39.0 35.0 41.0 29.5 41.0 48-49 35.7951704847574 39.0 35.0 41.0 28.0 41.0 50-51 35.58557445929053 39.0 35.0 41.0 27.0 41.0 52-53 35.395423207115954 39.0 35.0 41.0 27.0 41.0 54-55 35.1900393881691 39.0 35.0 40.0 26.0 41.0 56-57 34.98908899559166 39.0 35.0 40.0 26.0 41.0 58-59 34.72889532538882 38.0 34.0 40.0 25.0 41.0 60-61 34.41486095755589 38.0 34.0 40.0 23.5 41.0 62-63 34.03470292331246 38.0 34.0 40.0 22.5 41.0 64-65 33.64839838835571 37.5 33.0 40.0 22.0 41.0 66-67 33.249597189253834 37.0 33.0 39.5 20.5 41.0 68-69 32.799608927449924 36.0 32.5 39.0 18.5 40.0 70-71 32.32625613747133 36.0 32.0 39.0 16.5 40.0 72-73 31.964765346437304 35.0 32.0 38.0 13.5 40.0 74-75 31.51200606774444 35.0 31.0 37.5 9.0 39.5 76-77 29.37843492725328 33.0 28.5 35.5 6.5 37.5 78-79 30.398234856222146 34.0 30.0 36.0 4.0 39.0 80-81 30.41926980394203 35.0 31.0 36.0 2.0 38.0 82-83 30.094044019238602 34.0 30.5 35.5 2.0 37.0 84-85 29.684463243394006 34.0 30.0 35.0 2.0 37.0 86-87 29.154592042506316 34.0 29.5 35.0 2.0 36.0 88-89 28.61738095763615 34.0 29.0 35.0 2.0 36.0 90-91 28.196279694124684 34.0 29.0 35.0 2.0 35.5 92-93 27.93899348479954 34.0 28.5 35.0 2.0 35.0 94-95 27.59029782913601 34.0 27.5 35.0 2.0 35.0 96-97 27.100862205617076 33.0 26.5 35.0 2.0 35.0 98-99 26.431609457173643 33.0 25.0 35.0 2.0 35.0 100-101 23.568702558124137 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08090782683389364 1101 2 0.13250578996404982 1101 3 0.01599197398854102 1101 4 0.06164573589560973 1101 5 0.008893837347201838 1101 6 0.15465248074266924 1101 7 0.19441564646530907 1101 8 0.10864233357160913 1101 9 0.24207801328873302 1101 10-11 0.1432169381527899 1101 12-13 0.11701917294426067 1101 14-15 0.22655253277121545 1101 16-17 0.12743800101746672 1101 18-19 0.1335262370236805 1101 20-21 0.23614138535197782 1101 22-23 0.08308903936594447 1101 24-25 0.19851498912526466 1101 26-27 0.22350719230225025 1101 28-29 0.1280625115982943 1101 30-31 0.11146037393786656 1101 32-33 0.2410818565518369 1101 34-35 0.4266748647236298 1101 36-37 0.3407486283654322 1101 38-39 0.25096276876679724 1101 40-41 0.42385572197809296 1101 42-43 0.3508449887511276 1101 44-45 0.25108214060296774 1101 46-47 0.08755663658333646 1101 48-49 0.004091329272952748 1101 50-51 0.27806877969941723 1101 52-53 0.42911784772828554 1101 54-55 0.4179614663221116 1101 56-57 0.1734060467186609 1101 58-59 0.016227884932654035 1101 60-61 0.13853978476823414 1101 62-63 0.04816243116690799 1101 64-65 0.036801349442157516 1101 66-67 0.03128540352420117 1101 68-69 -0.13525205824822706 1101 70-71 -0.13608334761324414 1101 72-73 -0.25759041558759677 1101 74-75 -0.08842635244560526 1101 76-77 -0.02816735246683777 1101 78-79 -0.012219572836709602 1101 80-81 0.030910297669688447 1101 82-83 -0.06877289486951099 1101 84-85 -0.23104300939722222 1101 86-87 -0.07473950603571922 1101 88-89 0.27706919086638493 1101 90-91 0.22621906180297913 1101 92-93 0.11591273784540945 1101 94-95 0.09341274628236818 1101 96-97 -0.2228009218142084 1101 98-99 0.07547471842134712 1101 100-101 1.0572363820279698 1102 1 0.034606304761194195 1102 2 0.04795844344999978 1102 3 0.07795073869338154 1102 4 -0.029448242834170912 1102 5 -0.03394091282476097 1102 6 0.03274446649197671 1102 7 0.0634796952139638 1102 8 0.05858964304796643 1102 9 0.1748391730975456 1102 10-11 0.10568486086747697 1102 12-13 0.11468337337107215 1102 14-15 0.17368508801910565 1102 16-17 0.2740703269844147 1102 18-19 0.21921502783127522 1102 20-21 0.21074741896809002 1102 22-23 0.08643565923578933 1102 24-25 0.00404341555253751 1102 26-27 0.06487785139677271 1102 28-29 0.2469849132279549 1102 30-31 0.2467906928147343 1102 32-33 0.0749700203642476 1102 34-35 0.13341131510194515 1102 36-37 0.2355097793557519 1102 38-39 0.012878763757889544 1102 40-41 0.06876237818013209 1102 42-43 0.034550830123990295 1102 44-45 -0.06296109649116488 1102 46-47 -0.11318984924533737 1102 48-49 -0.18322017533912316 1102 50-51 -0.012250494009116153 1102 52-53 0.1719985643194022 1102 54-55 0.2115143728989466 1102 56-57 -0.013724127534850084 1102 58-59 -0.20522663807479802 1102 60-61 -0.19003082494268853 1102 62-63 -0.07872693196118519 1102 64-65 -0.09153094036895482 1102 66-67 -0.03845458307167604 1102 68-69 0.10392874740971791 1102 70-71 -0.19340744063076443 1102 72-73 -0.13074073569906375 1102 74-75 -0.17511438927164846 1102 76-77 -0.13349273298501174 1102 78-79 -0.22891982328204463 1102 80-81 -0.4222827701092804 1102 82-83 -0.25150759918528465 1102 84-85 -0.18500493994273626 1102 86-87 -0.0595755481175928 1102 88-89 -0.037708186159662205 1102 90-91 -0.18942429169532105 1102 92-93 -0.0363727962909941 1102 94-95 0.01497716981717545 1102 96-97 0.18554236907275623 1102 98-99 0.3926275782601465 1102 100-101 0.5254627837281802 1103 1 0.11056884378304588 1103 2 0.04812423860136761 1103 3 0.031679711614238215 1103 4 0.03633970953026022 1103 5 -0.004464280124281572 1103 6 -0.009395861651533721 1103 7 0.10079778411943607 1103 8 0.16074454371267421 1103 9 0.16906252098158348 1103 10-11 -0.027808050299050535 1103 12-13 -0.01912258521941368 1103 14-15 -0.023728398543852336 1103 16-17 -0.1835351983571627 1103 18-19 0.03227881315273606 1103 20-21 0.1391789968361863 1103 22-23 -0.04046744004278935 1103 24-25 0.02427206015536143 1103 26-27 -0.05059738077098075 1103 28-29 0.059178819383532755 1103 30-31 0.001707282582991354 1103 32-33 0.11965290441453647 1103 34-35 0.04843693699736917 1103 36-37 -0.2584834186800151 1103 38-39 -0.022528863255374176 1103 40-41 0.05553367031337331 1103 42-43 0.07636065872234354 1103 44-45 0.0629914075610074 1103 46-47 0.39969908763688267 1103 48-49 0.43811133424754445 1103 50-51 0.6931342489102335 1103 52-53 0.5787098866011817 1103 54-55 0.5103923105817927 1103 56-57 0.42930560433512 1103 58-59 0.42791995756173407 1103 60-61 0.4750755130132731 1103 62-63 0.5818653057716077 1103 64-65 0.617867245046746 1103 66-67 0.536359933602732 1103 68-69 0.7509563239427237 1103 70-71 0.7414672422314723 1103 72-73 0.7059085006615646 1103 74-75 0.9058040067911541 1103 76-77 0.8419968535524198 1103 78-79 0.8445143396814991 1103 80-81 0.7306768914940882 1103 82-83 0.7906992651702858 1103 84-85 0.7445659342122006 1103 86-87 0.6584412582751185 1103 88-89 0.6540878988932484 1103 90-91 0.8257176049575854 1103 92-93 0.7139233443905546 1103 94-95 0.36806030517801247 1103 96-97 0.6311070215464838 1103 98-99 0.7247125395491594 1103 100-101 0.9929963845152692 1104 1 -0.09618652980958586 1104 2 -0.0878115078865136 1104 3 0.023451682886410197 1104 4 -0.011198104640101292 1104 5 -0.040803220404825424 1104 6 0.018156071811830543 1104 7 -0.014108155210522 1104 8 -0.1363646490628838 1104 9 -0.17620998351920747 1104 10-11 0.0017421493666418542 1104 12-13 -0.14158009322856202 1104 14-15 -0.06826264426675266 1104 16-17 0.08111678681972734 1104 18-19 0.1153101266546841 1104 20-21 0.18639011459924149 1104 22-23 0.048717996915002004 1104 24-25 0.06673240060627705 1104 26-27 0.11088076758459664 1104 28-29 0.028922655067574965 1104 30-31 -0.05289446966847322 1104 32-33 -0.08551243937307618 1104 34-35 0.08494646752015456 1104 36-37 0.16142178035266141 1104 38-39 0.021938863002006315 1104 40-41 0.05522574956166437 1104 42-43 -0.04202253202329587 1104 44-45 -0.03335721022887839 1104 46-47 0.005506344266827057 1104 48-49 0.08012180999047303 1104 50-51 0.08038107260575345 1104 52-53 -0.021091306028459655 1104 54-55 -0.2618842001074597 1104 56-57 -0.012394375709035899 1104 58-59 -0.012340197922149798 1104 60-61 -0.14648398221127223 1104 62-63 -0.19676756900657466 1104 64-65 0.0040599497925910555 1104 66-67 -0.09898518440494541 1104 68-69 -0.13129993049637534 1104 70-71 -0.033724323100294384 1104 72-73 -0.1598552179053101 1104 74-75 -0.11781630439119795 1104 76-77 -0.2304181678303685 1104 78-79 -0.0010939808307419696 1104 80-81 -0.10634129836104833 1104 82-83 -0.07717590997975776 1104 84-85 -0.14720260379723626 1104 86-87 -0.1523242734836323 1104 88-89 0.023108389034419474 1104 90-91 -0.016829747245818538 1104 92-93 -0.06795932814699057 1104 94-95 0.17472845243923274 1104 96-97 0.3019923098010793 1104 98-99 0.17791126752531383 1104 100-101 0.5890783879957411 1105 1 -0.12297025086715863 1105 2 0.055345296136891875 1105 3 -0.0011838647503878974 1105 4 -0.09123230693064244 1105 5 -0.015470569432459058 1105 6 -0.1278639104130832 1105 7 -0.03713994396504461 1105 8 0.03125079419795895 1105 9 -0.02318843017921779 1105 10-11 0.08031369828391632 1105 12-13 0.03835470338995606 1105 14-15 -4.540760032796243E-4 1105 16-17 0.03672646792127665 1105 18-19 -0.06066318544964844 1105 20-21 0.09097067320856667 1105 22-23 -0.15419909622727346 1105 24-25 -0.05677072266939831 1105 26-27 -0.07051352332862848 1105 28-29 -0.08027300141001348 1105 30-31 -0.26556890345629114 1105 32-33 -0.12996857240819537 1105 34-35 -0.12934278192489757 1105 36-37 -0.2023261083298351 1105 38-39 -0.1518673212101831 1105 40-41 -0.04318323317291828 1105 42-43 -0.05183252397817739 1105 44-45 0.06442785449011268 1105 46-47 0.0059670432234923965 1105 48-49 0.08614301372576705 1105 50-51 -0.09680479915250828 1105 52-53 -0.10196213762429807 1105 54-55 0.12187223914767742 1105 56-57 -0.04968150646540437 1105 58-59 0.09977918576267797 1105 60-61 -0.021178078104050257 1105 62-63 -0.18473147969309878 1105 64-65 -0.25194019341897445 1105 66-67 -0.33182664920649074 1105 68-69 -0.30949091574979803 1105 70-71 -0.18658423692063764 1105 72-73 -0.3508020358707782 1105 74-75 -0.06549617179414113 1105 76-77 -0.27448152209786514 1105 78-79 -0.3098454708627969 1105 80-81 -0.2549780506598047 1105 82-83 -0.14200125505644223 1105 84-85 -0.14560411004589113 1105 86-87 -0.39935631917654746 1105 88-89 -0.22314441728036627 1105 90-91 -0.18779276075403928 1105 92-93 -0.3263651096819622 1105 94-95 -0.5218768017402269 1105 96-97 -0.5122464996896348 1105 98-99 -0.34773097314592505 1105 100-101 0.21310275923380217 1106 1 -0.019194287009952404 1106 2 0.042135038161781324 1106 3 -0.13899414900191687 1106 4 -0.057492536722165255 1106 5 -0.07340053853647532 1106 6 -0.015950732788724054 1106 7 -0.0631959805485991 1106 8 0.02893571449988741 1106 9 -0.033177707704830084 1106 10-11 0.024549414718968876 1106 12-13 0.0875403462934159 1106 14-15 0.13893809108083843 1106 16-17 0.0015319588453195365 1106 18-19 0.010206860703611653 1106 20-21 -0.0068099229271041395 1106 22-23 -0.08751526512531882 1106 24-25 -0.17441491452115798 1106 26-27 -0.08950883171631574 1106 28-29 -0.06702565977841601 1106 30-31 0.006257477221218721 1106 32-33 0.12576085251964741 1106 34-35 0.1674933464565953 1106 36-37 0.1514572722586749 1106 38-39 0.04734820052507871 1106 40-41 0.14051489738697853 1106 42-43 0.15020983191473647 1106 44-45 0.33220046174664475 1106 46-47 0.10389703351817303 1106 48-49 -0.03550833209310866 1106 50-51 -0.10249184275864565 1106 52-53 -0.11349886315862534 1106 54-55 0.008366583360292168 1106 56-57 -0.1306144676907337 1106 58-59 0.11451464155260993 1106 60-61 0.00856937159991844 1106 62-63 -0.07674811012373794 1106 64-65 -0.11200448588399325 1106 66-67 -0.21812656910579165 1106 68-69 -0.24720308724158002 1106 70-71 -0.356377055829487 1106 72-73 -0.2940677498053361 1106 74-75 -0.09132175994093572 1106 76-77 -0.09931157299783067 1106 78-79 0.049649852358353286 1106 80-81 -0.02155232604515689 1106 82-83 0.062358299600631995 1106 84-85 0.22825000496853676 1106 86-87 0.37430994062943057 1106 88-89 0.496095749193465 1106 90-91 0.36575392015274844 1106 92-93 0.4317270739308725 1106 94-95 0.67954784638885 1106 96-97 0.7185441662480727 1106 98-99 0.7229070352710032 1106 100-101 1.1727673958351623 1107 1 -0.06818498665617767 1107 2 0.05410946718570386 1107 3 0.008720631733382334 1107 4 0.06369188294985406 1107 5 0.058839951507273724 1107 6 -0.02538573708596914 1107 7 -0.0023055408410002087 1107 8 0.06775293588112419 1107 9 -0.15301772605096176 1107 10-11 -0.07849137281828433 1107 12-13 -0.018657364858064795 1107 14-15 -0.039216649722163766 1107 16-17 -0.13925200175257402 1107 18-19 0.10613589146179692 1107 20-21 0.1145812063384497 1107 22-23 -0.15700808499131824 1107 24-25 -0.1511245816924216 1107 26-27 -0.0744858306717191 1107 28-29 0.040725792185533294 1107 30-31 -0.1681265229302653 1107 32-33 -0.22362832852113002 1107 34-35 -0.30053293985039176 1107 36-37 -0.28165770982123206 1107 38-39 -0.20862717946816645 1107 40-41 -0.14746943686611047 1107 42-43 -0.08480687842569523 1107 44-45 -0.08015887842370972 1107 46-47 -0.038598987453646316 1107 48-49 -0.2357525965593581 1107 50-51 -0.07264255666941466 1107 52-53 -0.34004679190745435 1107 54-55 -0.38119421398120323 1107 56-57 -0.38154939466912197 1107 58-59 -0.6353146783886174 1107 60-61 -0.635267368847515 1107 62-63 -0.4681461507482254 1107 64-65 -0.2944764591137954 1107 66-67 -0.42255131156036896 1107 68-69 -0.36911335739471696 1107 70-71 -0.5680124390417234 1107 72-73 -0.33152005669492013 1107 74-75 -0.2927315930058434 1107 76-77 -0.2720608316309807 1107 78-79 -0.0999268926545831 1107 80-81 -0.31023305597723194 1107 82-83 -0.004648745356867323 1107 84-85 -0.21361883910931212 1107 86-87 -0.11295138342703837 1107 88-89 -0.16816526663954434 1107 90-91 -0.04780991103336518 1107 92-93 -0.1514443456538359 1107 94-95 -0.10623158553321588 1107 96-97 -0.1651414500252848 1107 98-99 -0.096583417691253 1107 100-101 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43.0 6 118.0 7 276.0 8 563.0 9 909.0 10 1555.0 11 2404.0 12 3242.0 13 4590.0 14 5318.0 15 7045.0 16 9076.0 17 11904.0 18 10708.0 19 7065.0 20 6411.0 21 6698.0 22 7129.0 23 7749.0 24 8823.0 25 10021.0 26 11661.0 27 13808.0 28 16843.0 29 20303.0 30 24716.0 31 29823.0 32 36446.0 33 45063.0 34 59064.0 35 82386.0 36 113372.0 37 194429.0 38 211626.0 39 25546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.206677556906875 23.906326477584702 16.013285824492566 20.873710141015852 2 14.182550017757784 24.26816862069173 40.56078479371876 20.988496567831724 3 17.29979352814057 29.879929289909363 32.64873899669525 20.17153818525482 4 11.365483282601584 20.073218252192635 40.91152610594876 27.649772359257025 5 10.522540346286817 40.64997501871089 36.611032299101275 12.216452335901023 6 25.07399076595241 41.701697323089334 19.073264402365297 14.151047508592962 7 23.179827157570735 36.49084119725587 24.389663966547147 15.939667678626249 8 22.438514927574328 36.42452540567005 22.677893866642055 18.45906580011357 9 24.615398506740934 18.75171809066703 24.171353584564173 32.46152981802786 10-11 21.287318935817147 29.018877427147938 30.12738450919291 19.566419127841996 12-13 23.798741103550956 27.73805964608837 29.576241088501988 18.88695816185869 14-15 21.653041476526834 29.208989866510425 28.643498910703798 20.494469746258943 16-17 17.941941917839006 32.21376787602611 30.50492311828928 19.33936708784561 18-19 17.622236814185392 31.293958776756508 33.1348536509142 17.948950758143894 20-21 18.467703139868703 30.5099446147487 32.41614835482076 18.606203890561833 22-23 18.724579782612622 29.9097764124461 31.913747333824265 19.451896471117013 24-25 18.64045604396707 30.77664721002141 32.41216919857216 18.170727547439366 26-27 18.810437378886967 29.97142196420447 33.15177560548365 18.066365051424913 28-29 17.990491057902414 29.41702299281863 34.20635748726355 18.386128462015396 30-31 18.33736980133354 30.765360483011722 33.043122320029376 17.854147395625365 32-33 17.848980582816484 30.948656929649076 32.69699602506556 18.50536646246887 34-35 18.390803444444163 30.046930928008308 32.7913994245882 18.770866202959333 36-37 19.18493778512954 29.687789268668602 32.34701654096258 18.780256405239285 38-39 18.77272636057152 29.719507545352386 33.02978006100498 18.47798603307112 40-41 17.85966552821579 29.628133917140577 34.22175310732876 18.290447447314875 42-43 17.773249578365775 30.206718657352088 32.980926381309835 19.0391053829723 44-45 17.855440857928 30.621624577241313 32.20811716748919 19.3148173973415 46-47 18.41970480515565 30.227147431379464 31.25716404066692 20.095983722797968 48-49 18.448080052490806 29.962849111007216 31.641661968044016 19.947408868457963 50-51 18.70593458190054 30.17218589555477 31.526848709671757 19.59503081287293 52-53 17.907119767724176 30.579505711585497 32.41884090831566 19.09453361237467 54-55 17.913189518693827 30.075515728179496 31.946905229617172 20.064389523509497 56-57 18.302105049832154 31.09357850445349 30.544592102702197 20.05972434301216 58-59 17.705437431464006 30.022352892783367 31.48105973579844 20.791149939954188 60-61 18.239789072058517 30.285576407452908 30.83436628564062 20.640268234847955 62-63 18.726686638321098 29.93224954000317 31.223601599605118 20.117462222070618 64-65 18.540932193357186 29.63232358093235 31.521320376505145 20.305423849205315 66-67 17.728760997799313 30.143563951648055 30.921053621655137 21.206621428897492 68-69 17.6778385927316 30.349404232500067 30.945899104028683 21.026858070739646 70-71 18.119897364124096 29.978253835677254 30.591410390502922 21.310438409695728 72-73 18.080132751974926 29.372327553860263 30.15045959552383 22.397080098640977 74-75 18.09758955641658 29.876066721110494 29.922969342239647 22.10337438023328 76-77 18.34930518070323 29.226246218350262 30.916910407853276 21.507538193093232 78-79 17.95812477262178 28.977506805665325 31.06614981245217 21.99821860926072 80-81 17.900766996573847 29.829695659371254 30.21590276349518 22.053634580559724 82-83 18.061271377060955 29.57784631189892 29.986626482574298 22.374255828465827 84-85 17.96932217435535 28.546941748449456 30.024499722095698 23.459236355099495 86-87 18.04581988844194 28.735620057542377 30.457570911911535 22.760989142104147 88-89 18.752214277662365 27.964716491940028 30.60000885711065 22.683060373286953 90-91 18.037265368619508 27.877849831892366 31.113896696800623 22.97098810268751 92-93 17.98983893589741 28.422938241036334 30.584973503781303 23.002249319284953 94-95 17.91871646272795 27.85445231505936 30.43339548560791 23.79343573660478 96-97 18.23735346685789 27.478428545701988 29.782527234885936 24.501690752554186 98-99 18.116195390288475 27.37805664145289 30.019207549384568 24.486540418874064 100-101 18.38781265707472 27.319689507483908 30.50276953144203 23.78972830399934 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3800.0 1 2369.5 2 1022.5 3 1089.0 4 890.0 5 877.5 6 1562.5 7 2813.5 8 3783.5 9 3841.0 10 3064.5 11 2301.5 12 2163.5 13 2087.0 14 1977.0 15 1891.5 16 1848.0 17 1958.5 18 2076.5 19 2202.0 20 2416.5 21 2706.5 22 3086.5 23 3590.0 24 4111.5 25 4929.5 26 6929.5 27 9744.5 28 10981.5 29 12316.0 30 15787.5 31 19092.0 32 24228.0 33 31030.0 34 36933.0 35 42219.0 36 49036.5 37 54492.0 38 53095.5 39 49437.5 40 45915.5 41 41670.5 42 38068.0 43 37341.0 44 37480.0 45 36507.0 46 34546.0 47 33267.0 48 44891.0 49 44528.0 50 29899.0 51 21747.5 52 14150.5 53 10343.0 54 8490.0 55 7257.5 56 6164.0 57 5226.5 58 4516.0 59 3940.5 60 3177.0 61 2465.0 62 1991.0 63 1442.0 64 1004.5 65 779.0 66 532.5 67 407.5 68 301.0 69 201.5 70 154.0 71 113.0 72 85.0 73 69.0 74 58.0 75 43.0 76 30.5 77 19.0 78 14.5 79 12.5 80 8.5 81 3.0 82 1.0 83 1.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38204316847020203 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.5048969346252708E-4 16-17 0.0020065292461670273 18-19 2.508161557708784E-4 20-21 5.016323115417569E-5 22-23 0.0 24-25 0.0 26-27 1.5048969346252708E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00687236266812207 36-37 0.016002070738182043 38-39 0.010634605004685245 40-41 0.007022852361584596 42-43 0.00290946740694219 44-45 0.001906202783858676 46-47 0.001454733703471095 48-49 0.0 50-51 3.511426180792298E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.022852361584596E-4 60-61 7.524484673126353E-4 62-63 0.0 64-65 0.0 66-67 0.0012540807788543923 68-69 6.52122005004284E-4 70-71 0.0018560395527045003 72-73 0.0 74-75 0.0 76-77 0.09867107568026358 78-79 0.03345887517983519 80-81 1.0032646230835138E-4 82-83 0.0 84-85 0.0 86-87 0.14843300098520587 88-89 0.3204427206128743 90-91 0.27489450672488275 92-93 0.0 94-95 5.016323115417569E-5 96-97 5.016323115417569E-5 98-99 2.508161557708784E-4 100-101 7.022852361584596E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 996746.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.989620447516614 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6988470443539 24.85556626158529 2 10.819092321663998 6.921973139133453 3 3.7289080225250624 3.5785905697282945 4 1.8646637288818955 2.385995397967314 5 1.0980175704519206 1.7562582661730632 6 0.6947111078882086 1.3334126797210535 7 0.4866708715673877 1.0897891525011985 8 0.384858856309203 0.984919099135739 9 0.28682472187026486 0.8257872588834863 >10 2.1633747997542296 14.481790399206979 >50 0.4005014582248353 9.087712786281573 >100 0.34687122009997157 21.56109281161793 >500 0.018503980095747507 3.978106200261635 >1k 0.006899789188244833 4.528305045239941 >5k 0.0012545071251354241 2.6307009325631214 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7443 0.7467298589610593 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6871 0.6893431225206823 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6004 0.6023600796993416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5903 0.5922271070061982 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4652 0.4667187026584506 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4305 0.4319054202374527 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3746 0.37582292780708426 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3010 0.30198265154813764 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2956 0.29656502258348666 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2588 0.25964488445401335 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2409 0.24168644770081846 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2322 0.2329580454799919 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1863 0.1869081992804586 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1719 0.17246118870805602 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1586 0.15911776922104529 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1564 0.15691058705026154 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1454 0.1458746761963429 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1394 0.13985508845784184 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1362 0.13664464166397458 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1323 0.13273190963394887 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1292 0.12962178930238996 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1223 0.12269926340311374 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1166 0.11698065505153771 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1088 0.1091551909914863 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1062 0.10654670297146918 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1051 0.1054431118860773 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0032646230835138E-4 0.0 14-15 0.0 0.0 0.0 1.5048969346252705E-4 0.0 16-17 0.0 0.0 0.0 4.013058492334055E-4 0.0 18-19 0.0 0.0 0.0 6.019587738501082E-4 0.0 20-21 0.0 0.0 0.0 0.0010032646230835138 0.0 22-23 0.0 0.0 0.0 0.003310773256175596 0.0 24-25 0.0 0.0 0.0 0.008678238989672394 0.0 26-27 0.0 0.0 0.0 0.016102397200490396 0.0 28-29 0.0 0.0 0.0 0.04263874648104933 0.0 30-31 0.0 0.0 0.0 0.11176367901150344 0.0 32-33 0.0 0.0 0.0 0.24098416246466 0.0 34-35 0.0 0.0 0.0 0.47253763747233496 0.0 36-37 0.0 0.0 0.0 0.8153029959488174 0.0 38-39 0.0 0.0 0.0 1.2155554173279852 0.0 40-41 0.0 0.0 0.0 1.6330639902241897 0.0 42-43 0.0 0.0 0.0 2.064869083999334 0.0 44-45 0.0 0.0 0.0 2.5496465498732874 0.0 46-47 0.0 0.0 0.0 3.143629369969882 0.0 48-49 0.0 0.0 0.0 3.7421770441015063 0.0 50-51 0.0 0.0 0.0 4.315994245274123 0.0 52-53 0.0 0.0 0.0 4.853392940628806 0.0 54-55 0.0 0.0 0.0 5.4364401763337895 0.0 56-57 0.0 0.0 0.0 6.105366863774723 0.0 58-59 0.0 5.016323115417569E-5 0.0 6.832633389047961 0.0 60-61 0.0 1.0032646230835138E-4 0.0 7.625864563288943 0.0 62-63 0.0 1.0032646230835138E-4 0.0 8.377159276285031 0.0 64-65 0.0 1.0032646230835138E-4 0.0 9.1251933792561 0.0 66-67 0.0 1.0032646230835138E-4 0.0 9.963872440922763 0.0 68-69 0.0 1.0032646230835138E-4 0.0 10.857931709783635 0.0 70-71 0.0 1.0032646230835138E-4 0.0 11.840629408093939 0.0 72-73 0.0 1.0032646230835138E-4 0.0 12.80556932257566 5.016323115417569E-5 74-75 0.0 1.0032646230835138E-4 0.0 13.80783068103609 1.0032646230835138E-4 76-77 0.0 1.0032646230835138E-4 0.0 14.823385295752377 1.0032646230835138E-4 78-79 0.0 1.0032646230835138E-4 0.0 15.932494336571203 1.0032646230835138E-4 80-81 0.0 1.0032646230835138E-4 0.0 17.091214813001507 1.0032646230835138E-4 82-83 0.0 1.0032646230835138E-4 0.0 18.22274681814625 1.0032646230835138E-4 84-85 0.0 1.5048969346252705E-4 0.0 19.44091072349425 1.0032646230835138E-4 86-87 0.0 2.0065292461670276E-4 0.0 20.727196296749625 1.0032646230835138E-4 88-89 0.0 2.0065292461670276E-4 0.0 21.96522484163468 1.0032646230835138E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 85 3.670284E-7 39.076397 5 TAAGGCG 60 0.00397116 31.633272 5 TGGGCGA 65 0.005873909 29.199945 6 CATTCCG 225 3.6379788E-12 27.415504 9 ATGGGAC 355 0.0 24.059109 5 CTCCCGC 160 1.8762839E-6 23.724955 7 CCTTTAG 315 0.0 22.686241 1 GAGTACT 5545 0.0 22.59112 12-13 CTTAGAG 170 3.1772506E-6 22.329369 3 TACTCCC 215 4.4696208E-8 22.069725 5 GTACATG 5335 0.0 22.056892 1 GGGCGTA 130 2.9621852E-4 21.89996 7 GTACTTT 5865 0.0 21.277624 14-15 GGGTATG 225 7.2293915E-8 21.08885 7 CGTCCGA 90 5.2193664E-6 21.08885 10-11 ACATGGG 5250 0.0 20.78758 3 GCGTGTA 80 5.030429E-5 20.760378 58-59 TGGGTAT 300 1.6916601E-10 20.561626 6 GTATAGG 580 0.0 20.53496 1 CCTCGTC 185 6.610726E-6 20.51888 8 >>END_MODULE