FastQCFastQC Report
Thu 26 May 2016
SRR938758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992321
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75440.7602378665774482No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA64890.6539214629137144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55330.5575816696411746No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA54810.5523414298397393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46890.47252854671018757No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT42190.4251648408125999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39800.40107989249446496No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30230.3046393253795899No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG28530.2875077721825901No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA25290.25485704726595526No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT24610.24800442598715539No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC21040.21202816427345586No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG17140.1727263657626917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.15226927576862728No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT14500.14612207138617442No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG12930.13030057813953347No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA12660.12757968439648057No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.12485879065342767No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG12280.12375027838773946No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11860.11951777700965716No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.11810694321696306No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA10810.10893652356445142No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG10710.10792878514109848No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT10570.1065179513484044No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA10170.1024869976549927No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA10110.10188235460098093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG801.0869777E-535.5792925
GTATGGT1703.6397978E-927.942041
GTAAGAC1109.558383E-525.8758473
GACATAT3250.024.8469221
GCTCTAT1151.2813069E-424.7833731
CATTCCG2151.6880222E-924.2699189
CATATAG3750.024.0357883
CGCCGGA901.9462095E-723.75794692-93
CATGGGG12950.023.4447844
GAGTACT47150.022.85417712-13
AGACGTG1050.002212452522.5900275
GTACATG52950.022.517221
GCCAAGC1503.060483E-522.1404638
GGGTATG1754.0900286E-621.6886187
ACATGGG52100.021.2155463
GTACTTT50800.021.16376714-15
AGTACTT53050.020.6256212-13
ATGGGAT11300.020.5709155
TACATGG55800.020.2338622
CCGTATC1201.4094985E-719.79828894-95
Produced by FastQC (version 0.11.5)