##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938758_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992321 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46752814865351 33.0 31.0 34.0 30.0 34.0 2 31.540183065761987 33.0 31.0 34.0 28.0 34.0 3 31.51869505936083 33.0 31.0 34.0 28.0 34.0 4 35.09733241561954 37.0 35.0 37.0 32.0 37.0 5 35.136151507425524 37.0 35.0 37.0 33.0 37.0 6 35.21150212481646 37.0 35.0 37.0 32.0 37.0 7 35.14373877001494 37.0 35.0 37.0 32.0 37.0 8 35.11988862474945 37.0 35.0 37.0 32.0 37.0 9 36.657547305760936 39.0 37.0 39.0 32.0 39.0 10-11 36.55723097666984 39.0 37.0 39.0 32.0 39.0 12-13 36.487710126058 39.0 37.0 39.0 32.0 39.0 14-15 37.56645531032801 40.0 37.0 41.0 32.0 41.0 16-17 37.46928161351015 40.0 37.0 41.0 32.0 41.0 18-19 37.50314867870377 40.0 37.0 41.0 32.0 41.0 20-21 37.42563293531025 40.0 37.0 41.0 32.0 41.0 22-23 37.4077742988408 40.0 37.0 41.0 32.0 41.0 24-25 37.3416087133095 40.0 37.0 41.0 32.0 41.0 26-27 37.176896387358525 40.0 36.5 41.0 31.0 41.0 28-29 37.00567709440796 39.5 36.0 41.0 31.0 41.0 30-31 36.777143686367616 39.0 36.0 41.0 30.0 41.0 32-33 36.568731287557156 39.0 35.0 41.0 30.0 41.0 34-35 36.291904534923674 39.0 35.0 41.0 30.0 41.0 36-37 36.054405781999975 39.0 35.0 41.0 30.0 41.0 38-39 35.859378164928486 39.0 35.0 40.5 29.0 41.0 40-41 35.72927258417387 39.0 35.0 40.5 28.5 41.0 42-43 35.68686493584234 39.0 35.0 41.0 27.5 41.0 44-45 35.48432865977844 39.0 35.0 41.0 26.5 41.0 46-47 35.133343948178066 39.0 35.0 41.0 24.5 41.0 48-49 34.83326463916414 39.0 35.0 41.0 23.5 41.0 50-51 33.720496694114104 38.0 33.5 40.0 21.5 40.5 52-53 33.80395607872856 38.0 33.5 39.5 22.0 40.5 54-55 34.34138247603346 38.5 34.0 40.0 22.0 41.0 56-57 34.29863925080694 39.0 34.0 40.0 20.5 41.0 58-59 34.148116385725984 38.5 34.0 40.0 19.5 41.0 60-61 33.87750133273407 38.0 34.0 40.0 17.5 41.0 62-63 33.47141348414475 38.0 33.5 40.0 13.0 41.0 64-65 33.053157697962654 37.5 33.0 40.0 8.0 41.0 66-67 32.64570385994048 37.0 32.0 40.0 6.5 41.0 68-69 32.182673751739614 36.5 32.0 39.0 2.0 41.0 70-71 31.70007638657249 36.0 31.0 39.0 2.0 40.5 72-73 31.191571074279395 35.5 31.0 38.5 2.0 40.0 74-75 30.63702521663857 35.0 30.5 37.5 2.0 40.0 76-77 30.07820705195194 35.0 29.5 37.0 2.0 39.0 78-79 29.540042486251927 35.0 29.0 36.5 2.0 39.0 80-81 28.977484100407025 34.0 29.0 36.0 2.0 38.5 82-83 28.489314445627976 34.0 28.5 35.5 2.0 37.0 84-85 28.045472180876953 34.0 27.5 35.0 2.0 37.0 86-87 27.52400281763663 34.0 26.0 35.0 2.0 36.0 88-89 26.953047451379142 34.0 25.5 35.0 2.0 36.0 90-91 26.389279779426214 34.0 24.5 35.0 2.0 36.0 92-93 25.84909167497211 33.0 21.5 35.0 2.0 35.0 94-95 25.269688941380863 33.0 18.5 35.0 2.0 35.0 96-97 24.595330543241552 32.5 4.5 35.0 2.0 35.0 98-99 23.801652388692773 32.0 2.0 35.0 2.0 35.0 100-101 21.51570157237426 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0587339644599254 1101 2 0.17755345673320022 1101 3 0.4011505594931606 1101 4 0.02354629820430887 1101 5 0.02284121656260396 1101 6 0.23582787652499348 1101 7 0.2151507917519595 1101 8 0.40153026936268077 1101 9 0.5089108332091001 1101 10-11 0.3392075727721746 1101 12-13 0.37596402090974124 1101 14-15 0.24892172332338447 1101 16-17 0.42310373604678375 1101 18-19 0.4787312209231871 1101 20-21 0.5488465506842104 1101 22-23 0.49261317792748116 1101 24-25 0.4789298174677725 1101 26-27 0.4207424318221129 1101 28-29 0.5305276136939057 1101 30-31 0.5063611209330432 1101 32-33 0.485369606562152 1101 34-35 0.2804927751468611 1101 36-37 0.46723595598512446 1101 38-39 0.392287352199709 1101 40-41 0.43046399449060857 1101 42-43 0.5310564727450071 1101 44-45 0.609902879802263 1101 46-47 0.7136205030894089 1101 48-49 0.6136741978509761 1101 50-51 0.5546229755901635 1101 52-53 0.5785572019418979 1101 54-55 0.5493730909614172 1101 56-57 0.6332164845128645 1101 58-59 0.5252202046988756 1101 60-61 0.2835410520939021 1101 62-63 0.22584169121189035 1101 64-65 0.14768638218490082 1101 66-67 -0.1417609910874802 1101 68-69 0.23921265427862437 1101 70-71 0.07765873438374982 1101 72-73 -0.15840994729815705 1101 74-75 -0.15722941804007107 1101 76-77 0.06774516448128054 1101 78-79 -0.11341206766199363 1101 80-81 -0.047651672191456385 1101 82-83 0.011474676943631579 1101 84-85 -0.005013906466675877 1101 86-87 -0.207683237058351 1101 88-89 -0.3464716762343052 1101 90-91 -0.5221541894469226 1101 92-93 -0.8086854913121009 1101 94-95 -0.8017575157812011 1101 96-97 -0.7854617254696663 1101 98-99 -1.0542859270448801 1101 100-101 -0.5624152399692406 1102 1 0.10453508129841182 1102 2 0.018189195564815464 1102 3 0.019181057774950716 1102 4 0.09530317105654262 1102 5 0.0344176770436988 1102 6 0.17121232669681063 1102 7 0.14207304261106657 1102 8 0.1483623140362198 1102 9 0.27278540365583837 1102 10-11 0.18773635284090773 1102 12-13 0.0971356274389521 1102 14-15 0.3266923418800829 1102 16-17 0.18292439755881418 1102 18-19 0.3903560061465541 1102 20-21 0.4553381743955427 1102 22-23 0.37567804047044007 1102 24-25 0.2728838552684607 1102 26-27 0.329483840756815 1102 28-29 0.26882765664922914 1102 30-31 0.39932181680932644 1102 32-33 0.349598558452179 1102 34-35 0.5253971995454805 1102 36-37 0.6307292979095322 1102 38-39 0.669832455292493 1102 40-41 0.6817687625318456 1102 42-43 0.4876447459408837 1102 44-45 0.4535508609019203 1102 46-47 0.5317691285189667 1102 48-49 0.22558356211214203 1102 50-51 0.4029101319475501 1102 52-53 0.37451940125461647 1102 54-55 0.20963404456966117 1102 56-57 0.27239711166063785 1102 58-59 0.1655874727401141 1102 60-61 0.35628052803891563 1102 62-63 0.134153546881997 1102 64-65 0.08231911414366522 1102 66-67 -0.06424810448953622 1102 68-69 0.31970019723394927 1102 70-71 0.032829481806427196 1102 72-73 -0.079345299531834 1102 74-75 -0.2443159730228892 1102 76-77 -0.17522090081081387 1102 78-79 -0.14810923261044806 1102 80-81 -0.05558766875503096 1102 82-83 -0.028087178726469375 1102 84-85 0.08038665195256911 1102 86-87 -0.06504470613051438 1102 88-89 -0.14490917623430377 1102 90-91 -0.3495919042235549 1102 92-93 -0.5330948555732711 1102 94-95 -0.3171677391488963 1102 96-97 -0.5756518027892525 1102 98-99 -0.9063048273885208 1102 100-101 -0.8068095698661466 1103 1 0.12444229779325866 1103 2 0.11222012339986875 1103 3 0.32410889282649435 1103 4 0.1819629648709764 1103 5 0.2947578832292663 1103 6 0.14211954319166153 1103 7 0.24560912508528787 1103 8 0.29223860269601687 1103 9 0.24574416654243691 1103 10-11 0.30499923943884966 1103 12-13 0.42560985424307063 1103 14-15 0.5766717233233791 1103 16-17 0.5532079027134529 1103 18-19 0.30633538758985424 1103 20-21 0.40103405068420983 1103 22-23 0.43940484459414364 1103 24-25 0.5114714841344323 1103 26-27 0.43265909848877726 1103 28-29 0.6181317803605708 1103 30-31 0.5345486209330446 1103 32-33 0.6110779398954804 1103 34-35 0.5082219418135239 1103 36-37 0.6155901226517955 1103 38-39 0.6361623521997117 1103 40-41 0.564026494490605 1103 42-43 0.8246189727450144 1103 44-45 1.046757046468926 1103 46-47 0.8220371697560793 1103 48-49 0.9953825311843119 1103 50-51 0.9534771422568298 1103 52-53 0.9913905352752366 1103 54-55 0.7836855909614115 1103 56-57 0.7627373178462094 1103 58-59 0.8193452046988696 1103 60-61 0.49527021876057375 1103 62-63 0.3595916912118895 1103 64-65 0.45106138218490344 1103 66-67 0.5014890089125288 1103 68-69 0.6245043209452916 1103 70-71 0.30722123438374993 1103 72-73 0.41986088603517757 1103 74-75 0.17687474862659514 1103 76-77 0.3485368311479462 1103 78-79 0.39396293233800606 1103 80-81 0.26305666114187787 1103 82-83 0.34547467694363476 1103 84-85 0.3983402601999906 1103 86-87 0.15625426294165123 1103 88-89 0.2395908237656954 1103 90-91 0.1793874772197448 1103 92-93 0.2771061753545645 1103 94-95 0.3499508175521342 1103 96-97 0.32110077453033625 1103 98-99 0.5183807396217865 1103 100-101 1.0198347600307613 1104 1 0.018058611992653795 1104 2 0.11710531977448824 1104 3 0.16108573069154986 1104 4 0.27469811089312657 1104 5 0.17204187114467828 1104 6 0.0057831887102892665 1104 7 0.2002244322353448 1104 8 0.12520537006761856 1104 9 0.16375725818392795 1104 10-11 0.09589500983160093 1104 12-13 0.23741448667006182 1104 14-15 0.30405138092660167 1104 16-17 0.30652361268324313 1104 18-19 0.21615411870767787 1104 20-21 0.1831161252058635 1104 22-23 -0.029148529021163938 1104 24-25 0.06679172582627757 1104 26-27 0.2087396624364075 1104 28-29 0.16516853766168538 1104 30-31 0.19268255849598148 1104 32-33 0.04382567403445847 1104 34-35 0.35374460042379496 1104 36-37 0.36803372788845223 1104 38-39 0.3995097842238735 1104 40-41 0.2775199486698625 1104 42-43 -0.08300288022578428 1104 44-45 0.08373690548202717 1104 46-47 0.09075418889000986 1104 48-49 0.2828805170856228 1104 50-51 0.258970596436086 1104 52-53 0.2944937578331377 1104 54-55 0.366588914224252 1104 56-57 0.47663761996101073 1104 58-59 0.25761257630008316 1104 60-61 0.400800933765602 1104 62-63 0.45525382615650045 1104 64-65 0.6339999521748325 1104 66-67 0.28353482965773935 1104 68-69 0.19816595236522971 1104 70-71 0.26446796147337537 1104 72-73 0.1314550451489751 1104 74-75 -0.09384982337743253 1104 76-77 -0.18017464921459236 1104 78-79 -0.40295448961567004 1104 80-81 -0.20389852516225204 1104 82-83 -0.14953992527187765 1104 84-85 -0.2586219754495538 1104 86-87 -0.12360877834737494 1104 88-89 0.13449666465189836 1104 90-91 0.012006812567175729 1104 92-93 -0.1543394439807848 1104 94-95 -0.19080900117898025 1104 96-97 -0.43838059505677407 1104 98-99 -0.36910214053934354 1104 100-101 -0.230145602507001 1105 1 0.16844737393538978 1105 2 0.2628749457348931 1105 3 0.35772310602446566 1105 4 0.4951304775613323 1105 5 0.3858594060719085 1105 6 0.3843631980139932 1105 7 0.43067003879087196 1105 8 0.416827435183329 1105 9 0.3006832528368477 1105 10-11 0.4909154830936657 1105 12-13 0.502960108050182 1105 14-15 0.6551082715467302 1105 16-17 0.49398201438857825 1105 18-19 0.630954676929953 1105 20-21 0.5645746598212682 1105 22-23 0.481884540025618 1105 24-25 0.48130397144407766 1105 26-27 0.49099412386949126 1105 28-29 0.5014693438123459 1105 30-31 0.46187349402948286 1105 32-33 0.6295373307584242 1105 34-35 0.6748006220165692 1105 36-37 0.8040089043776888 1105 38-39 0.7626395095601097 1105 40-41 0.7461127889068493 1105 42-43 0.6815402925419676 1105 44-45 0.8072088231186711 1105 46-47 0.8227681342230824 1105 48-49 0.9358876073264426 1105 50-51 0.8346700356578438 1105 52-53 0.7160783525341188 1105 54-55 0.5149901594893294 1105 56-57 0.57485406911524 1105 58-59 0.6253629711963313 1105 60-61 0.8143971223138706 1105 62-63 0.6975739247144261 1105 64-65 0.7580766106112975 1105 66-67 0.6927250495216555 1105 68-69 0.7399053361737202 1105 70-71 0.6586628587492278 1105 72-73 0.6715207845123352 1105 74-75 0.5618722105555314 1105 76-77 0.5378540900311961 1105 78-79 0.5704553181248109 1105 80-81 0.6076835646951757 1105 82-83 0.6224518343040408 1105 84-85 0.5615813769512563 1105 86-87 0.5962745675101786 1105 88-89 0.5020451384865048 1105 90-91 0.3430372234126402 1105 92-93 0.3555858707860331 1105 94-95 0.5120802591764999 1105 96-97 0.5963038202156135 1105 98-99 0.6800837853070654 1105 100-101 1.0706748615536057 1106 1 -0.0631700282504788 1106 2 0.10609885103406214 1106 3 0.17575501608693145 1106 4 -0.10212053413498978 1106 5 0.04158293293105686 1106 6 0.08223485134275421 1106 7 0.15265286265984201 1106 8 0.15835391084711148 1106 9 0.13377001147285483 1106 10-11 0.10425271856409069 1106 12-13 -0.01243785947462328 1106 14-15 0.20778106725976642 1106 16-17 0.2739186382999321 1106 18-19 0.38774691840496445 1106 20-21 0.31779846420309354 1106 22-23 0.21441776705935212 1106 24-25 0.09545359149029764 1106 26-27 0.20504080823032922 1106 28-29 0.043544305211469236 1106 30-31 0.10197804439228975 1106 32-33 0.12203917249985352 1106 34-35 0.10540683246959048 1106 36-37 0.22449966539533506 1106 38-39 0.03285022496313417 1106 40-41 0.012692498466748248 1106 42-43 -0.022922776757965835 1106 44-45 -0.12731651217917772 1106 46-47 -0.22496879445863982 1106 48-49 -0.18260852249362358 1106 50-51 -0.23597613806126105 1106 52-53 -0.38448123410846335 1106 54-55 -0.3913979080445529 1106 56-57 -0.08445353702456515 1106 58-59 0.10297244127939109 1106 60-61 0.14314695832319302 1106 62-63 -0.030631966839798963 1106 64-65 -0.03657678878925452 1106 66-67 -0.10869687577933007 1106 68-69 -0.330036434521908 1106 70-71 -0.22753820895621502 1106 72-73 -0.10395024716561707 1106 74-75 -0.22748807443503338 1106 76-77 -0.13306456050215587 1106 78-79 -0.010249791320045887 1106 80-81 -0.054520873649376966 1106 82-83 0.017911058653375278 1106 84-85 0.19047047888786395 1106 86-87 0.09028209594363545 1106 88-89 0.09107491919312949 1106 90-91 -0.05190278122956116 1106 92-93 -0.029972353472476954 1106 94-95 0.3177649328602854 1106 96-97 0.4414486870551855 1106 98-99 0.387256485148626 1106 100-101 0.6383924141063098 1107 1 0.05983287679227445 1107 2 0.04630550122126209 1107 3 -0.017015739165653798 1107 4 0.16953705711827638 1107 5 0.17549291757666907 1107 6 0.10177999461462406 1107 7 0.09844425756811859 1107 8 0.24855656147913407 1107 9 0.1663732146287913 1107 10-11 0.057932016671429665 1107 12-13 0.15085617416456643 1107 14-15 0.2248601433430153 1107 16-17 0.20485412449951923 1107 18-19 0.24387218837493663 1107 20-21 -0.013173505743658609 1107 22-23 0.14215214586990754 1107 24-25 0.14791800032678282 1107 26-27 0.0822017873994767 1107 28-29 0.11442471460983228 1107 30-31 0.10043772790065475 1107 32-33 0.19972906845288918 1107 34-35 0.15633970432579503 1107 36-37 0.29758178114051503 1107 38-39 0.35071779871589825 1107 40-41 0.17660451215498085 1107 42-43 0.21128678432499015 1107 44-45 0.3475843281176054 1107 46-47 0.47963088908876017 1107 48-49 0.47320048996743225 1107 50-51 0.24147027277695088 1107 52-53 0.18399554018200348 1107 54-55 0.27721748497515364 1107 56-57 0.2647481127431561 1107 58-59 0.4233280309991656 1107 60-61 0.35035952199904585 1107 62-63 0.20245479229137686 1107 64-65 0.22948532722906378 1107 66-67 -0.14155858676951993 1107 68-69 0.20970353586187684 1107 70-71 0.30674822162614035 1107 72-73 -0.21607728864588083 1107 74-75 0.13394197139401598 1107 76-77 0.1714303542097717 1107 78-79 -0.06279565451184865 1107 80-81 -0.1498770974449748 1107 82-83 -0.270824145431245 1107 84-85 -0.15943255628676312 1107 86-87 -0.32002836708779014 1107 88-89 -0.155851276332438 1107 90-91 0.04136294336302271 1107 92-93 0.05589273080108015 1107 94-95 0.2152270687788267 1107 96-97 0.31943737904456526 1107 98-99 0.4726972263735014 1107 100-101 0.5456134646431252 1108 1 0.1595619559129169 1108 2 -0.0027884236086812564 1108 3 -0.006087688370087108 1108 4 0.12648433239234436 1108 5 0.020971558442944627 1108 6 0.07688022695234054 1108 7 0.15353220200837114 1108 8 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-2.004186496674034 2316 94-95 -2.1343507910269537 2316 96-97 -2.4024872504920083 2316 98-99 -2.614685837679378 2316 100-101 -2.637521361506323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6198.0 3 1196.0 4 899.0 5 993.0 6 1224.0 7 1402.0 8 1811.0 9 2112.0 10 2701.0 11 3483.0 12 4834.0 13 6138.0 14 6933.0 15 8252.0 16 10165.0 17 12317.0 18 10761.0 19 7789.0 20 7905.0 21 8373.0 22 9268.0 23 10227.0 24 11686.0 25 13158.0 26 14878.0 27 16885.0 28 19599.0 29 22514.0 30 26425.0 31 30916.0 32 37087.0 33 45776.0 34 58805.0 35 82114.0 36 109889.0 37 168238.0 38 178860.0 39 30509.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.609597636460265 23.11736260005537 15.4405063342299 20.83253342925446 2 14.067718768768012 23.60981715225872 41.05630781809098 21.26615626088229 3 17.485596117767514 29.830323983852303 32.407371879663714 20.276708018716462 4 11.251232680647645 19.2230553926561 41.62357541482164 27.902136511874616 5 10.825239531089798 40.25677934855625 36.49832568147037 12.419655438883586 6 25.235633071726355 41.16932564768173 19.484318221516794 14.110723059075116 7 23.432992023533405 36.38646667582573 24.584104445647696 15.59643685499317 8 22.477827471003213 35.61829002546848 23.163587246969495 18.740295256558813 9 25.124965308439513 18.6479626592658 24.44122618897439 31.7858458433203 10-11 21.327832590537827 28.609736233963673 30.498474942453758 19.56395623304475 12-13 23.875218321483338 27.63496799466384 29.7359540831861 18.75385960066672 14-15 21.878988944897102 28.954648488308184 28.929512930427503 20.236849636367214 16-17 17.998572957545488 31.938109613435223 30.748065356927373 19.31525207209192 18-19 17.699012695217313 31.223254918973737 33.34341731997937 17.734315065829577 20-21 18.61562322349774 30.398522152068086 32.57439978835958 18.411454836074594 22-23 18.816430623232453 29.833401606533215 31.888062931895337 19.462104838339 24-25 18.699916619235754 30.579428132195126 32.47849953006783 18.242155718501287 26-27 18.81243537463121 29.784223952026174 33.14835835762447 18.254982315718156 28-29 18.102320982761835 29.356739453168636 34.14832513876512 18.39261442530441 30-31 18.417568378858597 30.536762164270975 33.12743704859644 17.918232408273987 32-33 17.872303691801164 30.972894684898755 32.67464383667449 18.480157786625597 34-35 18.61010198673889 29.873598744280695 32.66078789913791 18.85551136984251 36-37 19.2634591003421 29.663394747998854 32.30426921134989 18.768876940309156 38-39 18.869486795724335 29.806747328973636 32.894682934009225 18.429082941292805 40-41 17.91068320883561 29.698242605219704 34.1528070890387 18.23826709690598 42-43 17.794202142079687 30.22647819278717 32.853246905637626 19.126072759495514 44-45 17.923017974612474 30.61399981284349 32.174682782359746 19.2882994301843 46-47 18.369170097236285 30.239463708131286 31.30237541442529 20.08899078020714 48-49 18.425341307936844 30.1068435637593 31.62745561339802 19.840359514905835 50-51 18.802840237883835 30.17982945418763 31.590690050313796 19.426640257614736 52-53 18.027531127962728 30.512410458570503 32.42842883154348 19.031629581923294 54-55 17.98153089027908 30.06704082448275 32.211669312482485 19.739758972755688 56-57 18.38090368237138 31.120835413319092 30.765783741162274 19.732477163147255 58-59 17.999785606077463 29.71929565528693 31.723575443401014 20.55734329523459 60-61 18.41295034941175 29.80101853841002 31.436796279681044 20.34923483249719 62-63 19.09959189619555 29.327529100411343 31.73780999803738 19.83506900535573 64-65 18.95763590069504 29.076211255803784 32.00816072086368 19.9579921226375 66-67 18.090471332628947 29.582619837019227 31.48993211158475 20.83697671876708 68-69 17.868892362673204 29.71568329275618 31.69820315160741 20.71722119296321 70-71 18.191101909986756 29.438683620711032 31.421440452773584 20.948774016528624 72-73 18.130665674896836 28.779591805754794 31.02448305262705 22.065259466721315 74-75 18.095088710840358 29.29570195788733 30.970291418842166 21.638917912430145 76-77 18.552415507571766 28.52901270105621 31.7368565366477 21.18171525472432 78-79 18.130604033159035 28.29194354406782 32.03869599278597 21.538756429987167 80-81 18.14655819945645 29.130304809856135 31.144262300057207 21.57887469063021 82-83 18.252934430533557 28.6953741648704 31.075193154150494 21.976498250445545 84-85 18.067908760250305 27.741720078714266 31.276779797346176 22.91359136368925 86-87 18.359473206314036 27.802353726733998 31.62097563566556 22.217197431286408 88-89 19.230644702720806 26.989356976851248 31.766373550989684 22.013624769438263 90-91 18.591363141625227 26.981896530349637 32.20582810584283 22.220912222182303 92-93 18.701531461603153 27.402567042557692 31.636679380776627 22.259222115062528 94-95 18.567552276842328 26.844503910199514 31.517766687549404 23.070177125408755 96-97 18.86412251133666 26.17641417916301 30.927716105016366 24.03174720448396 98-99 18.97461795875943 26.028395459681676 31.176103566460423 23.820883015098467 100-101 19.542028988439935 25.64663970953889 31.712195815389038 23.099135486632143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4670.0 1 2873.0 2 1206.5 3 1288.0 4 1060.5 5 1088.5 6 1735.0 7 2847.5 8 3934.5 9 3990.5 10 3139.0 11 2500.5 12 2357.0 13 2325.5 14 2282.5 15 2173.5 16 2092.0 17 2181.5 18 2262.5 19 2372.0 20 2576.0 21 2878.0 22 3236.0 23 3692.0 24 4297.0 25 5224.5 26 7273.5 27 9911.0 28 11111.0 29 12397.5 30 15570.0 31 18898.0 32 24089.5 33 30885.5 34 36839.5 35 41894.0 36 48422.0 37 54207.5 38 52987.0 39 49094.5 40 45487.5 41 41551.0 42 37935.0 43 36425.0 44 36190.0 45 35253.5 46 33774.5 47 32751.5 48 44423.0 49 43417.5 50 26277.0 51 18466.5 52 13944.5 53 11414.0 54 9293.0 55 7636.5 56 6491.5 57 5585.0 58 4867.5 59 4144.0 60 3426.5 61 2796.5 62 2241.5 63 1708.0 64 1272.0 65 969.0 66 692.0 67 531.5 68 414.0 69 290.5 70 228.0 71 179.5 72 141.0 73 121.5 74 109.0 75 81.5 76 61.5 77 56.5 78 42.0 79 25.0 80 23.0 81 18.0 82 9.5 83 8.0 84 6.0 85 4.5 86 3.0 87 1.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2639266930761316 2 0.1618427907904801 3 0.19781905250417958 4 0.1606335046824566 5 0.23843091096530256 6 0.1918733958063973 7 0.24276418618572015 8 0.248911390568173 9 0.14571897601683326 10-11 0.20462128686181183 12-13 0.21117158661360588 14-15 0.1705597281524829 16-17 0.28841473676360774 18-19 0.23203177197701147 20-21 0.19771827866184433 22-23 0.24442695458425245 24-25 0.2302178428149762 26-27 0.3475185952932569 28-29 0.3173872164350044 30-31 0.2929495596686959 32-33 0.18839669824582972 34-35 0.35925874792531853 36-37 0.40430465544919436 38-39 0.38258789242593877 40-41 0.34404189773268934 42-43 0.3778011349150124 44-45 0.38732426301569756 46-47 0.39342108047698277 48-49 0.4132231404958678 50-51 0.4054635546360502 52-53 0.40526200695137965 54-55 0.3998706063864415 56-57 0.37613836651648 58-59 0.35139838822316566 60-61 0.318294181016022 62-63 0.38767697146387103 64-65 0.42052924406517656 66-67 0.42617257923595286 68-69 0.43186630132789694 70-71 0.43106011058921456 72-73 0.4220912386213735 74-75 0.4170525465046089 76-77 0.40087834480979445 78-79 0.37200663898073305 80-81 0.38490569079965054 82-83 0.3956884919295268 84-85 0.3966458434317121 86-87 0.3918590859207857 88-89 0.40047524944045326 90-91 0.4227966555177206 92-93 0.42123466096152357 94-95 0.42969966371768814 96-97 0.42985082448119105 98-99 0.4273314784228087 100-101 0.3992155764112621 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 992321.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.40760375926951 #Duplication Level Percentage of deduplicated Percentage of total 1 79.85521580595469 27.476266235622447 2 9.65388542792298 6.643341290827198 3 3.4785575423783346 3.5906648871591638 4 1.7050060441678707 2.346606894995507 5 0.9469493076059525 1.629112827811012 6 0.6394587177954385 1.3201345309389596 7 0.4475780232491149 1.0780061092718864 8 0.33972807235865965 0.9351383119693764 9 0.25594310221124567 0.7925749961222482 >10 1.975061470302164 14.161770200881874 >50 0.36718331329681053 8.967032338899582 >100 0.3133845417692601 20.872325956599067 >500 0.014405105578506926 3.2883527886314794 >1k 0.00646759842300311 4.365116621261892 >5k 0.0011759269860005654 2.5335560090083566 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7544 0.7602378665774482 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6489 0.6539214629137144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5533 0.5575816696411746 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5481 0.5523414298397393 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4689 0.47252854671018757 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4219 0.4251648408125999 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3980 0.40107989249446496 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3023 0.3046393253795899 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2853 0.2875077721825901 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2529 0.25485704726595526 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2461 0.24800442598715539 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2104 0.21202816427345586 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1714 0.1727263657626917 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1511 0.15226927576862728 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1450 0.14612207138617442 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1293 0.13030057813953347 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1266 0.12757968439648057 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1239 0.12485879065342767 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1228 0.12375027838773946 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1186 0.11951777700965716 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1172 0.11810694321696306 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1081 0.10893652356445142 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1071 0.10792878514109848 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1057 0.1065179513484044 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1017 0.1024869976549927 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1011 0.10188235460098093 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.0386921167646354E-5 0.0 12-13 0.0 0.0 0.0 1.0077384233529271E-4 0.0 14-15 0.0 0.0 0.0 1.0077384233529271E-4 0.0 16-17 0.0 0.0 0.0 1.0077384233529271E-4 0.0 18-19 0.0 0.0 0.0 1.5116076350293905E-4 0.0 20-21 0.0 0.0 0.0 9.573515021852807E-4 0.0 22-23 0.0 0.0 0.0 0.003527084481735245 0.0 24-25 0.0 0.0 0.0 0.008364228913829295 0.0 26-27 0.0 0.0 0.0 0.018441613147358565 5.0386921167646354E-5 28-29 0.0 0.0 0.0 0.04166998380564353 1.0077384233529271E-4 30-31 0.0 0.0 0.0 0.10863420203744555 1.0077384233529271E-4 32-33 0.0 0.0 0.0 0.23288834963686145 1.0077384233529271E-4 34-35 0.0 0.0 0.0 0.4520714567161231 1.0077384233529271E-4 36-37 0.0 0.0 0.0 0.7720284061306775 1.0077384233529271E-4 38-39 0.0 0.0 0.0 1.1608138898602367 1.0077384233529271E-4 40-41 0.0 0.0 0.0 1.5600798531926665 1.0077384233529271E-4 42-43 0.0 0.0 0.0 1.9523420344827933 1.0077384233529271E-4 44-45 0.0 0.0 0.0 2.427742635699537 1.0077384233529271E-4 46-47 0.0 0.0 0.0 3.0008938639815144 1.0077384233529271E-4 48-49 0.0 0.0 0.0 3.58392092881235 1.0077384233529271E-4 50-51 0.0 0.0 0.0 4.1309717319294865 1.0077384233529271E-4 52-53 0.0 0.0 0.0 4.64491832783948 1.0077384233529271E-4 54-55 0.0 0.0 0.0 5.227239975773968 1.0077384233529271E-4 56-57 0.0 0.0 0.0 5.89280081747741 1.0077384233529271E-4 58-59 0.0 0.0 0.0 6.602097506754367 1.0077384233529271E-4 60-61 0.0 0.0 0.0 7.391962882978391 1.0077384233529271E-4 62-63 0.0 0.0 0.0 8.126604193602676 1.0077384233529271E-4 64-65 0.0 0.0 0.0 8.875908098286743 1.0077384233529271E-4 66-67 0.0 0.0 0.0 9.706234172208388 1.0077384233529271E-4 68-69 0.0 0.0 0.0 10.60810967418809 1.0077384233529271E-4 70-71 1.0077384233529271E-4 0.0 0.0 11.577957132822949 1.0077384233529271E-4 72-73 1.0077384233529271E-4 0.0 0.0 12.519789463288593 1.0077384233529271E-4 74-75 1.0077384233529271E-4 0.0 0.0 13.498253085443118 1.0077384233529271E-4 76-77 1.0077384233529271E-4 0.0 0.0 14.509014724066104 1.0077384233529271E-4 78-79 1.0077384233529271E-4 0.0 0.0 15.597825703577772 1.0077384233529271E-4 80-81 1.0077384233529271E-4 0.0 0.0 16.753197805951906 1.0077384233529271E-4 82-83 1.0077384233529271E-4 0.0 0.0 17.8570744748927 1.0077384233529271E-4 84-85 1.0077384233529271E-4 0.0 0.0 19.02378363452955 1.0077384233529271E-4 86-87 1.0077384233529271E-4 0.0 0.0 20.278921840815624 1.0077384233529271E-4 88-89 1.0077384233529271E-4 0.0 0.0 21.509068134202543 1.0077384233529271E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 80 1.0869777E-5 35.579292 5 GTATGGT 170 3.6397978E-9 27.94204 1 GTAAGAC 110 9.558383E-5 25.875847 3 GACATAT 325 0.0 24.846922 1 GCTCTAT 115 1.2813069E-4 24.783373 1 CATTCCG 215 1.6880222E-9 24.269918 9 CATATAG 375 0.0 24.035788 3 CGCCGGA 90 1.9462095E-7 23.757946 92-93 CATGGGG 1295 0.0 23.444784 4 GAGTACT 4715 0.0 22.854177 12-13 AGACGTG 105 0.0022124525 22.590027 5 GTACATG 5295 0.0 22.51722 1 GCCAAGC 150 3.060483E-5 22.140463 8 GGGTATG 175 4.0900286E-6 21.688618 7 ACATGGG 5210 0.0 21.215546 3 GTACTTT 5080 0.0 21.163767 14-15 AGTACTT 5305 0.0 20.62562 12-13 ATGGGAT 1130 0.0 20.570915 5 TACATGG 5580 0.0 20.233862 2 CCGTATC 120 1.4094985E-7 19.798288 94-95 >>END_MODULE