##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938758_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992321 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.944482682519062 31.0 31.0 34.0 30.0 34.0 2 32.15388770367653 33.0 31.0 34.0 30.0 34.0 3 32.219472328006766 34.0 31.0 34.0 30.0 34.0 4 35.87005817673918 37.0 35.0 37.0 35.0 37.0 5 35.78071914229367 37.0 35.0 37.0 35.0 37.0 6 35.80721460092047 37.0 35.0 37.0 33.0 37.0 7 35.728296589510855 37.0 35.0 37.0 33.0 37.0 8 35.73284148980017 37.0 35.0 37.0 33.0 37.0 9 37.33159128951216 39.0 37.0 39.0 34.0 39.0 10-11 37.26808814889537 39.0 37.0 39.0 33.0 39.0 12-13 37.21518591262303 39.0 37.0 39.0 33.0 39.0 14-15 38.34789448172517 40.0 38.0 41.0 33.0 41.0 16-17 38.28332162677198 40.0 38.0 41.0 33.0 41.0 18-19 38.463192354087035 40.0 38.0 41.0 33.5 41.0 20-21 38.485956157332154 40.0 38.0 41.0 34.0 41.0 22-23 38.400314011292714 40.0 38.0 41.0 34.0 41.0 24-25 38.32059484783653 40.0 38.0 41.0 33.0 41.0 26-27 38.096641107061124 40.0 37.0 41.0 33.0 41.0 28-29 37.915403886444004 40.0 37.0 41.0 33.0 41.0 30-31 37.67913306278916 40.0 37.0 41.0 33.0 41.0 32-33 37.48229202042484 40.0 36.0 41.0 32.0 41.0 34-35 37.40796980009493 40.0 36.0 41.0 32.0 41.0 36-37 37.24326352057449 40.0 36.0 41.0 32.0 41.0 38-39 37.072410540540815 40.0 35.5 41.0 31.0 41.0 40-41 36.89869759886166 40.0 35.0 41.0 31.0 41.0 42-43 36.70603363226214 39.0 35.0 41.0 31.0 41.0 44-45 36.43864636544022 39.0 35.0 41.0 30.0 41.0 46-47 36.06773060330276 39.0 35.0 41.0 29.5 41.0 48-49 35.74121529222903 39.0 35.0 41.0 27.5 41.0 50-51 35.5482918329855 39.0 35.0 41.0 27.0 41.0 52-53 35.338756309702205 39.0 35.0 40.5 27.0 41.0 54-55 35.109474152013306 39.0 35.0 40.0 26.0 41.0 56-57 34.88690302835474 38.5 34.5 40.0 25.5 41.0 58-59 34.62288009625918 38.0 34.0 40.0 24.5 41.0 60-61 34.2802974037635 38.0 34.0 40.0 23.0 41.0 62-63 33.91740021626067 38.0 33.5 40.0 22.5 41.0 64-65 33.49484088314165 37.0 33.0 40.0 21.5 41.0 66-67 33.08842451182632 37.0 33.0 39.0 19.5 41.0 68-69 32.76254810691299 36.0 32.0 39.0 18.5 40.0 70-71 32.34671996259275 36.0 32.0 39.0 16.0 40.0 72-73 31.83596336266188 35.0 31.5 38.0 12.0 40.0 74-75 31.311364467747836 35.0 31.0 37.0 7.5 39.5 76-77 29.284936527595406 33.0 28.5 35.5 4.5 37.5 78-79 30.193782556249438 34.0 30.0 36.0 2.0 39.0 80-81 30.22039944735625 34.0 30.0 36.0 2.0 38.0 82-83 29.882608047194406 34.0 30.0 35.5 2.0 37.0 84-85 29.461820318223637 34.0 30.0 35.0 2.0 37.0 86-87 28.887235078165233 34.0 29.0 35.0 2.0 36.0 88-89 28.269380069554103 34.0 28.5 35.0 2.0 36.0 90-91 27.74829717399914 33.5 27.0 35.0 2.0 35.5 92-93 27.47712786487437 33.0 27.0 35.0 2.0 35.0 94-95 27.155647718832917 33.0 26.0 35.0 2.0 35.0 96-97 26.576971564644907 33.0 25.5 35.0 2.0 35.0 98-99 25.82802188001665 33.0 24.5 35.0 2.0 35.0 100-101 22.466669555516813 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0419090248248537 1101 2 0.06144822120301541 1101 3 -0.1323708028291719 1101 4 0.022223716039306396 1101 5 -0.14616450229380007 1101 6 0.017291764412178168 1101 7 0.14619420428438445 1101 8 0.09403309897128764 1101 9 0.19378447397711085 1101 10-11 0.22168544107011456 1101 12-13 0.06062765986460761 1101 14-15 0.2110225731109523 1101 16-17 0.15400474041330625 1101 18-19 0.312979339127466 1101 20-21 0.30740053758869834 1101 22-23 0.22836874056512357 1101 24-25 0.15186412184466036 1101 26-27 0.06007223252841243 1101 28-29 0.16277988834443846 1101 30-31 0.16193718633694232 1101 32-33 0.13726493290918285 1101 34-35 0.09207554553658781 1101 36-37 -0.05041298592690424 1101 38-39 0.022984652676370843 1101 40-41 -0.00445172562105256 1101 42-43 -0.09246223905612538 1101 44-45 -0.002547577585751526 1101 46-47 0.20732794718337288 1101 48-49 0.03103177286249803 1101 50-51 0.06051251032994287 1101 52-53 0.048342425987712545 1101 54-55 -0.010225294554061293 1101 56-57 -0.05510884290001883 1101 58-59 0.07355529741546718 1101 60-61 0.07260178300452225 1101 62-63 0.06376945065652961 1101 64-65 -0.13361886910928433 1101 66-67 -0.10905200658621794 1101 68-69 -0.29946518213110096 1101 70-71 -0.046895648655635114 1101 72-73 -0.299812018664376 1101 74-75 -0.2001428438098607 1101 76-77 -0.38992197103868165 1101 78-79 -0.5036979124800389 1101 80-81 -0.35820194692150764 1101 82-83 -0.1966926781185343 1101 84-85 -0.065819954674744 1101 86-87 0.00543312524522932 1101 88-89 0.015334483878710614 1101 90-91 -0.10399057120786281 1101 92-93 -0.11778701325594554 1101 94-95 -0.11450495549614104 1101 96-97 -0.32944864055924583 1101 98-99 -0.3250707964011532 1101 100-101 -0.03929462925106364 1102 1 0.17644363840539157 1102 2 0.25587476759476857 1102 3 0.14074990163818057 1102 4 0.09016830366817175 1102 5 -0.0549273888917341 1102 6 -0.15836330431633883 1102 7 0.034896953425274546 1102 8 0.05754254914311474 1102 9 0.14169040181216275 1102 10-11 0.16744897199794906 1102 12-13 0.16793543477869832 1102 14-15 0.15651226383260308 1102 16-17 0.3058894053617607 1102 18-19 0.3015317446257484 1102 20-21 0.39377832968491333 1102 22-23 0.12778347424210068 1102 24-25 0.27911927648383994 1102 26-27 0.2313029026315121 1102 28-29 0.32918345363653856 1102 30-31 0.28187919664622285 1102 32-33 0.23774818033185596 1102 34-35 0.5406365421001667 1102 36-37 0.5091875295370158 1102 38-39 0.51027524545988 1102 40-41 0.5234316506676038 1102 42-43 0.46940953929438933 1102 44-45 0.41999859939019046 1102 46-47 0.6371980073208263 1102 48-49 0.6463399773298448 1102 50-51 0.5342775446942056 1102 52-53 0.4239545393897757 1102 54-55 0.54720060750779 1102 56-57 0.5010391381996371 1102 58-59 0.6068403060065322 1102 60-61 0.4745938362691291 1102 62-63 0.5971564781479302 1102 64-65 0.4348143353580767 1102 66-67 0.5287057253725536 1102 68-69 0.4724849896902015 1102 70-71 0.664267366808275 1102 72-73 0.5046553352875129 1102 74-75 0.3847905754341241 1102 76-77 0.5565556921915586 1102 78-79 0.4340974053893767 1102 80-81 0.7282188005011747 1102 82-83 0.7307342978264799 1102 84-85 0.9362257927479334 1102 86-87 1.002281278166194 1102 88-89 0.8947964684147891 1102 90-91 0.8429005370395579 1102 92-93 0.688752935197666 1102 94-95 0.8246112386619373 1102 96-97 0.826371806176148 1102 98-99 0.7784569097844134 1102 100-101 1.1521948466939556 1103 1 0.05429930850847953 1103 2 0.12482322120301603 1103 3 0.057337530504156575 1103 4 -0.003151283960690421 1103 5 -0.027164502293800297 1103 6 0.05875009774551643 1103 7 0.005360870951051311 1103 8 0.04244976563795433 1103 9 0.17165947397710823 1103 10-11 0.09224794107012002 1103 12-13 0.047981826531270144 1103 14-15 0.08802257311095474 1103 16-17 0.029317240413313073 1103 18-19 -0.18143732753920006 1103 20-21 0.17954637092203285 1103 22-23 0.1935979072317906 1103 24-25 0.16261412184465485 1103 26-27 0.2684472325284162 1103 28-29 0.21642572167777985 1103 30-31 0.2263121863369406 1103 32-33 0.24313993290918035 1103 34-35 0.21799221220325649 1103 36-37 0.22033701407309536 1103 38-39 0.17017215267637198 1103 40-41 0.1527357743789466 1103 42-43 0.3636002609438762 1103 44-45 0.4169315890809173 1103 46-47 0.31422378051670563 1103 48-49 0.1719276061958297 1103 50-51 0.1447208436632792 1103 52-53 0.330217425987712 1103 54-55 0.2130872054459303 1103 56-57 0.08374532376664945 1103 58-59 0.24970113074880373 1103 60-61 0.31983094967119285 1103 62-63 0.2878111173231943 1103 64-65 0.25090196422404887 1103 66-67 0.3619479934137857 1103 68-69 0.5017014845355661 1103 70-71 0.41391685134436784 1103 72-73 0.34202131466895835 1103 74-75 0.3393988228568041 1103 76-77 0.4654113622946525 1103 78-79 0.38344792085329615 1103 80-81 0.4191105530784931 1103 82-83 0.3422239885481311 1103 84-85 0.4031175453252551 1103 86-87 0.4937039585785641 1103 88-89 0.36164698387870686 1103 90-91 0.28544692879213684 1103 92-93 0.34308798674405594 1103 94-95 0.34576587783719503 1103 96-97 0.6671346927740878 1103 98-99 0.5706167035988443 1103 100-101 1.2101845374156035 1104 1 0.011108976182196528 1104 2 -0.024091179602628188 1104 3 -0.03221030433974903 1104 4 -0.058349672681735854 1104 5 -0.18186440158886086 1104 6 -0.12336470587986526 1104 7 -0.06283147547392787 1104 8 -0.23296211754431795 1104 9 -0.09247244948713984 1104 10-11 -0.007278242212862551 1104 12-13 -0.20886661254223782 1104 14-15 -0.19470502004111268 1104 16-17 -0.16464298113753983 1104 18-19 -0.08051889853618377 1104 20-21 -0.03056994327736362 1104 22-23 -0.11319564765240386 1104 24-25 -0.06888738872935818 1104 26-27 -0.0630804613285818 1104 28-29 -0.12583258647932638 1104 30-31 -0.27065659513335305 1104 32-33 -0.23640387373734484 1104 34-35 -0.12122379988134213 1104 36-37 -0.12112673215046499 1104 38-39 -0.06400710210711225 1104 40-41 0.16654997377472114 1104 42-43 0.06524326195092556 1104 44-45 0.06262897075261975 1104 46-47 0.07239749652879368 1104 48-49 -0.2201247603701333 1104 50-51 -0.3546719055814336 1104 52-53 -0.39547995568398875 1104 54-55 -0.6021484441008909 1104 56-57 -0.4742128836855173 1104 58-59 -0.2646165933196798 1104 60-61 -0.11850943300756 1104 62-63 -0.2632477950635135 1104 64-65 -0.5691126379914593 1104 66-67 -0.5190092069890326 1104 68-69 -0.39164796158729587 1104 70-71 -0.47939029870599015 1104 72-73 -0.4344545161467508 1104 74-75 -0.4386404520676699 1104 76-77 -0.34065208181410966 1104 78-79 -0.4626276078476117 1104 80-81 -0.08505712063752213 1104 82-83 -0.057121932902020944 1104 84-85 -0.1482988695790759 1104 86-87 -0.05603622269032016 1104 88-89 -0.1878802064536167 1104 90-91 -0.30958126858953605 1104 92-93 -0.056070623527848085 1104 94-95 -0.2504456025253461 1104 96-97 -0.19004154086136182 1104 98-99 0.02280653240045538 1104 100-101 0.6028582534276872 1105 1 0.12256326789934135 1105 2 0.11320900800504319 1105 3 0.04866240360060914 1105 4 0.0986355180697629 1105 5 -0.18296653275065466 1105 6 0.04778563074043518 1105 7 0.0706654394789652 1105 8 0.12653098391206896 1105 9 0.051826986667464325 1105 10-11 0.0863824588366171 1105 12-13 0.05323817170894074 1105 14-15 0.07073069493836925 1105 16-17 0.005598966301640473 1105 18-19 0.13961851002424908 1105 20-21 0.12411743691188093 1105 22-23 0.1137298869272172 1105 24-25 0.12480193910354132 1105 26-27 0.021223882274611583 1105 28-29 0.08996633081483907 1105 30-31 0.012555841159276326 1105 32-33 0.08058663341679306 1105 34-35 0.06518256753320628 1105 36-37 -0.012310194048730239 1105 38-39 0.3135224064834716 1105 40-41 0.12993120585102247 1105 42-43 0.12610533708600968 1105 44-45 -0.01734506066527075 1105 46-47 -0.11507317379801663 1105 48-49 -0.0754429521798059 1105 50-51 -0.1731116436463651 1105 52-53 -0.08657191411381149 1105 54-55 -0.2256995965845192 1105 56-57 -0.08895772191862505 1105 58-59 -0.23003237178927094 1105 60-61 -0.10193554779073821 1105 62-63 -0.07839954257529058 1105 64-65 -0.07021732511604739 1105 66-67 -0.26914083907352193 1105 68-69 -0.03273506368778101 1105 70-71 0.13439400870476703 1105 72-73 -0.05480355842749063 1105 74-75 0.1048962847857382 1105 76-77 0.18160933183779804 1105 78-79 -0.11014091163401574 1105 80-81 -0.05254680732760164 1105 82-83 0.11803870936031302 1105 84-85 -0.013321540969162271 1105 86-87 0.07965319715724561 1105 88-89 0.19751246611221163 1105 90-91 0.14673119275152757 1105 92-93 0.2183646364902465 1105 94-95 0.22088262910622802 1105 96-97 0.4488402765304329 1105 98-99 0.24884259192371871 1105 100-101 0.8062505272633196 1106 1 0.088995133558182 1106 2 -0.0201310531508625 1106 3 0.03369737145843743 1106 4 0.017421280651632287 1106 5 -0.10630366730374163 1106 6 -0.09957992213519873 1106 7 0.01875053297888485 1106 8 0.13417143561807876 1106 9 0.07803521950394554 1106 10-11 0.03927676810788938 1106 12-13 -0.05705892893591624 1106 14-15 -0.07478756605405579 1106 16-17 -0.052248365948514675 1106 18-19 -0.25986277485530707 1106 20-21 -0.11832738653323815 1106 22-23 -0.029793742867610717 1106 24-25 0.015892451864537804 1106 26-27 -0.10172572969822369 1106 28-29 -0.05591523259657549 1106 30-31 0.013011987529786495 1106 32-33 0.15461707008611825 1106 34-35 0.029132371248984157 1106 36-37 -0.1603160674378401 1106 38-39 -0.006448125653648162 1106 40-41 0.32260157954793556 1106 42-43 0.102539624760972 1106 44-45 0.08413337834533507 1106 46-47 0.17510151411511288 1106 48-49 0.021096592279327808 1106 50-51 0.027052851615565032 1106 52-53 0.019397346464849363 1106 54-55 0.1771846209528931 1106 56-57 -0.04145845356933364 1106 58-59 0.06492478880048935 1106 60-61 -0.013791316730397796 1106 62-63 0.23650594833710414 1106 64-65 0.24098248112265708 1106 66-67 0.25384560772790365 1106 68-69 -0.012346229182128354 1106 70-71 -0.1811805641625952 1106 72-73 0.013740002541720031 1106 74-75 0.15877655645521216 1106 76-77 0.17835172014753553 1106 78-79 0.03083758288112648 1106 80-81 -0.2490474986113682 1106 82-83 1.056982896798786E-4 1106 84-85 0.03530144194553486 1106 86-87 -0.07703560404568677 1106 88-89 -0.16979536204574686 1106 90-91 -0.3373622163370875 1106 92-93 -0.22824302717244294 1106 94-95 -0.18842497703358418 1106 96-97 -0.02222117203704599 1106 98-99 0.16403519266445343 1106 100-101 0.862619925089561 1107 1 -0.04315309580947613 1107 2 0.04653470304796059 1107 3 -0.0031560906930891974 1107 4 -0.0549628639410642 1107 5 -0.006174315836489086 1107 6 0.03056560314296064 1107 7 -0.050914889598509205 1107 8 -0.06350901748275106 1107 9 -0.09044945634673951 1107 10-11 -0.11751506187394511 1107 12-13 -0.09901670143732133 1107 14-15 -0.09531942885175937 1107 16-17 -0.03202476154939404 1107 18-19 -0.08036176326049826 1107 20-21 -0.14152968403380584 1107 22-23 -0.14331867765535833 1107 24-25 -0.20669206068723156 1107 26-27 -0.18599928366393215 1107 28-29 -0.022129234161276656 1107 30-31 0.13978618044881586 1107 32-33 0.03566103202596338 1107 34-35 -0.006879721064656508 1107 36-37 0.07431100818497072 1107 38-39 0.17280218211699605 1107 40-41 -0.007173450351174893 1107 42-43 0.0834378468123731 1107 44-45 0.08497133392880585 1107 46-47 0.12945390809275636 1107 48-49 0.3273113157149581 1107 50-51 0.04427825288800591 1107 52-53 0.11799711195434526 1107 54-55 0.17804598856663745 1107 56-57 -0.03289599124807552 1107 58-59 -0.11541417837779022 1107 60-61 0.17903016458777898 1107 62-63 0.1925957100611626 1107 64-65 0.17074151280109362 1107 66-67 0.1698856774177102 1107 68-69 0.23902435009003398 1107 70-71 0.40088250394495617 1107 72-73 0.1830797052479589 1107 74-75 0.4171034352218683 1107 76-77 0.12179703452232715 1107 78-79 0.18001416226644196 1107 80-81 0.35807473364767617 1107 82-83 0.4224089738278174 1107 84-85 0.42766170626735445 1107 86-87 0.23567059253342038 1107 88-89 0.06814207710736753 1107 90-91 0.16206223791872887 1107 92-93 0.19328082285788994 1107 94-95 0.3072437973661408 1107 96-97 0.4926420529311031 1107 98-99 0.4949871648353543 1107 100-101 0.9057439093488746 1108 1 0.001974522183694205 1108 2 0.004583904963695318 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-1.7907846674114403 2316 94-95 -1.566525453708831 2316 96-97 -1.714463162453793 2316 98-99 -1.6884686404583498 2316 100-101 -1.75963226329932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 6.0 5 50.0 6 117.0 7 264.0 8 505.0 9 900.0 10 1564.0 11 2412.0 12 3298.0 13 4491.0 14 5355.0 15 7123.0 16 9033.0 17 12082.0 18 10691.0 19 7383.0 20 6678.0 21 6804.0 22 7340.0 23 8184.0 24 9180.0 25 10571.0 26 12159.0 27 14519.0 28 17345.0 29 20991.0 30 25413.0 31 30360.0 32 37037.0 33 46289.0 34 60188.0 35 83826.0 36 115839.0 37 197019.0 38 196725.0 39 20575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.05689384878948 24.023014513365872 15.992278663089504 20.92781297475515 2 14.218786058140461 24.339301496189236 40.403861250542924 21.038051195127384 3 17.29430295237126 29.893955685710573 32.60930686743503 20.202434494483136 4 11.290701295246196 20.030312771774454 40.87376967735239 27.80521625562696 5 10.492086180097616 40.66550276091615 36.66131753862158 12.181093520364655 6 25.215840207198536 41.678953123346616 18.942440931788752 14.162765737666094 7 23.2213164893215 36.45413127405346 24.407021518238555 15.917530718386491 8 22.419055930490234 36.45937151385489 22.73044710330629 18.391125452348582 9 24.656134456491397 18.776484625438744 24.113265767831177 32.454115150238685 10-11 21.259854422107363 28.98537872321557 30.115859686532886 19.63890716814418 12-13 23.771692829235704 27.75548436443449 29.604785145129448 18.868037661200358 14-15 21.622035611460404 29.16908943779281 28.676204574930896 20.53267037581589 16-17 17.90856233563961 32.230836944916 30.47772660004323 19.382874119401166 18-19 17.604333678315786 31.396493674929786 33.056490792797895 17.942681853956532 20-21 18.51794933292755 30.552865453819884 32.405088675942565 18.524096537310005 22-23 18.736578183873966 29.958299784041653 31.804778897151227 19.50034313493315 24-25 18.704698848906727 30.83826497063692 32.307653548385474 18.149382632070886 26-27 18.819317600642947 30.024866283888812 33.09644439853574 18.059371716932503 28-29 18.012618900537227 29.472015607852704 34.12988337443226 18.38548211717781 30-31 18.325471294067142 30.794773062345755 32.97914686880556 17.90060877478155 32-33 17.913507826600465 31.00780896504256 32.6263376467897 18.452345561567274 34-35 18.449564200385687 30.065310733666145 32.73770441413082 18.747420651817347 36-37 19.202939451176125 29.697487698531887 32.29757760356718 18.801995246724815 38-39 18.72734822555221 29.799656519478006 32.983553652705716 18.48944160226407 40-41 17.771787987632074 29.753417506177914 34.15964218478519 18.315152321404817 42-43 17.771514782594675 30.269751110609445 32.89283525892721 19.065898847868677 44-45 17.88054109127262 30.660663051463732 32.10650907848605 19.352286778777597 46-47 18.47710963331274 30.14329090401555 31.232862079355687 20.146737383316015 48-49 18.445075634469426 29.985674774552795 31.611031078634554 19.958218512343226 50-51 18.74087051512087 30.143688455493965 31.535123984640045 19.580317044745122 52-53 17.88816320525314 30.635852712983 32.34553133512241 19.13045274664146 54-55 17.88302373929404 30.187711436117947 31.89990940431574 20.02935542027227 56-57 18.325147874879637 31.116532812124454 30.483917047974497 20.074402265021412 58-59 17.72672098742789 29.95100852501044 31.536441786911524 20.785828700650146 60-61 18.25784014261859 30.307206410983806 30.780451820372328 20.654501626025272 62-63 18.77585818566045 29.871114060453802 31.211368607539065 20.141659146346687 64-65 18.544966426720762 29.61124934118171 31.453270006157368 20.390514225940155 66-67 17.71744125578977 30.123162290832717 30.94446258470494 21.214933868672574 68-69 17.662103788144847 30.302135552107966 30.99813114626892 21.03762951347827 70-71 18.11763437316875 29.942039895376542 30.647457147161628 21.29286858429308 72-73 18.02350247550944 29.403338234301195 30.139591926402847 22.433567363786516 74-75 18.069358814412315 29.932340237706057 29.969878607462526 22.028422340419098 76-77 18.348598412619985 29.283692498817683 30.89366005947381 21.474049029088523 78-79 17.922332438717827 28.955772288313437 31.069093279686676 22.05280199328206 80-81 17.89346495860173 29.866866469416877 30.157803190931887 22.08186538104951 82-83 18.12335516951044 29.47783135067698 29.99238647866828 22.406427001144298 84-85 17.957394834937485 28.63972444400552 29.930687751241784 23.47219296981521 86-87 18.056884492370795 28.759055755155284 30.41008706336544 22.77397268910848 88-89 18.736158723417166 28.0140865010264 30.530746594666745 22.719008180889684 90-91 18.100754531081037 27.919116776809926 31.032885173499665 22.947243518609373 92-93 18.102719398578074 28.445554083128844 30.38626847321667 23.06545804507641 94-95 17.87757660468892 27.955691645373943 30.406215343158472 23.76051640677867 96-97 18.286581804971277 27.471013649319953 29.73767044014509 24.504734105563678 98-99 18.152442606777445 27.429984853691497 29.939253527840286 24.478319011690775 100-101 18.44111215245236 27.238514173998045 30.52236700224728 23.798006671302314 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3787.0 1 2343.0 2 1001.5 3 1080.0 4 864.0 5 862.0 6 1554.0 7 2700.0 8 3661.5 9 3705.5 10 2941.5 11 2268.5 12 2088.0 13 2049.0 14 1928.5 15 1837.5 16 1862.0 17 1963.0 18 2047.5 19 2218.0 20 2429.0 21 2700.5 22 3031.5 23 3421.0 24 3965.0 25 4913.0 26 7033.0 27 9768.0 28 10908.5 29 12305.0 30 15737.0 31 19138.5 32 24293.0 33 31002.5 34 36991.0 35 42190.0 36 48765.0 37 54079.0 38 52887.0 39 49127.5 40 45525.0 41 41633.0 42 38033.0 43 37109.5 44 37334.5 45 36437.0 46 34688.0 47 33646.5 48 44896.5 49 44293.5 50 29537.0 51 21142.5 52 13792.5 53 10279.5 54 8412.5 55 7192.5 56 6147.0 57 5276.0 58 4497.5 59 3800.0 60 3174.5 61 2564.5 62 2021.5 63 1500.0 64 1066.5 65 768.0 66 528.0 67 417.5 68 297.0 69 182.0 70 144.0 71 95.5 72 68.0 73 78.0 74 60.0 75 38.0 76 27.5 77 15.5 78 16.0 79 13.0 80 8.0 81 3.5 82 2.0 83 2.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23550846953757906 2 0.0 3 0.0 4 0.0 5 0.02610042516484081 6 0.003627858324070538 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0027712806642205498 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.5270844817352447E-4 26-27 0.0013604468715264517 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00649991283062638 36-37 0.016426136300652712 38-39 0.012596730291911589 40-41 0.0077595858598175395 42-43 0.007356490490476369 44-45 0.0030232152700587814 46-47 4.0309536934117084E-4 48-49 0.0015619945561970371 50-51 5.0386921167646354E-5 52-53 0.0 54-55 0.0 56-57 0.0012596730291911588 58-59 5.5425613284411E-4 60-61 0.0024185722160470252 62-63 9.573515021852808E-4 64-65 0.0014108337926940979 66-67 0.005341013643770514 68-69 1.5116076350293908E-4 70-71 5.0386921167646354E-5 72-73 0.0 74-75 3.0232152700587815E-4 76-77 0.06288287761722265 78-79 0.14032757545189511 80-81 9.069645810176345E-4 82-83 7.558038175146954E-4 84-85 0.0 86-87 0.10394821836885444 88-89 0.3457550530523893 90-91 0.3789096471807006 92-93 6.046430540117563E-4 94-95 2.519346058382318E-4 96-97 5.0386921167646354E-5 98-99 0.0 100-101 0.00110851226568822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 992321.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.753553065929275 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61117524484933 24.644305716464597 2 10.782137181521033 6.847423303151144 3 3.8582999533719122 3.675441969410024 4 1.8620048228390818 2.3650107580414805 5 1.0890520152100605 1.7290635478264933 6 0.7080355057936977 1.348958580346935 7 0.49565224413824666 1.1017103885543522 8 0.36021960660611413 0.9150601915004328 9 0.27591032796770404 0.7885019916504395 >10 2.1721667282434804 14.485473521752471 >50 0.40843106787723826 9.243163752593878 >100 0.34921966709084934 21.588203723298395 >500 0.01941877820650936 4.091797844766363 >1k 0.007003493779396818 4.525551261399492 >5k 0.001273362505344876 2.65033344924354 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7329 0.7385714904753603 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7059 0.7113625530448312 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6014 0.6060538878044505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5817 0.5862014408643976 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4570 0.46053645947228766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4265 0.42980043756002345 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3661 0.3689330367895066 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3082 0.3105849820773722 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2951 0.29738360873144876 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2449 0.24679513987913185 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2435 0.24538430608643777 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2290 0.2307720989478203 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1708 0.17212172270867995 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1656 0.16688148290724475 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1650 0.16627683985323297 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1574 0.15861802783575074 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1435 0.14461046375114506 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1417 0.14279653458910976 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1343 0.13533927025629813 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1333 0.1343315318329452 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1287 0.1296959350855217 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1181 0.11901390779798068 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1172 0.11810694321696306 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1117 0.11256438188852196 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1111 0.11195973883451021 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1083 0.10913807124912202 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.0386921167646354E-5 0.0 12-13 0.0 0.0 0.0 1.0077384233529271E-4 0.0 14-15 0.0 0.0 0.0 1.0077384233529271E-4 0.0 16-17 0.0 0.0 0.0 1.0077384233529271E-4 0.0 18-19 0.0 0.0 0.0 1.5116076350293905E-4 0.0 20-21 0.0 0.0 0.0 9.573515021852807E-4 0.0 22-23 0.0 0.0 0.0 0.0033759237182323055 0.0 24-25 0.0 0.0 0.0 0.008213068150326356 0.0 26-27 0.0 0.0 0.0 0.018441613147358565 0.0 28-29 0.0 0.0 0.0 0.041972305332649416 0.0 30-31 0.0 0.0 0.0 0.11009542275130729 0.0 32-33 0.0 0.0 0.0 0.23712085101494376 0.0 34-35 5.0386921167646354E-5 0.0 0.0 0.4628038709248318 0.0 36-37 1.0077384233529271E-4 0.0 0.0 0.7891599593276772 0.0 38-39 1.0077384233529271E-4 0.0 0.0 1.184999612020707 0.0 40-41 1.0077384233529271E-4 0.0 0.0 1.5888507851793925 0.0 42-43 1.0077384233529271E-4 0.0 0.0 1.9889229392505046 0.0 44-45 1.0077384233529271E-4 0.0 0.0 2.4707730663767067 0.0 46-47 1.0077384233529271E-4 0.0 0.0 3.0512807851491606 0.0 48-49 1.0077384233529271E-4 0.0 0.0 3.640908536652958 0.0 50-51 1.0077384233529271E-4 0.0 0.0 4.195567764866409 0.0 52-53 1.0077384233529271E-4 0.0 0.0 4.7135957013909815 0.0 54-55 1.0077384233529271E-4 0.0 0.0 5.301661458338582 0.0 56-57 1.0077384233529271E-4 0.0 0.0 5.9722609921587875 0.0 58-59 1.0077384233529271E-4 0.0 0.0 6.689216493453228 0.0 60-61 1.0077384233529271E-4 0.0 0.0 7.4885042239356014 0.0 62-63 1.0077384233529271E-4 0.0 0.0 8.233273305714583 0.0 64-65 1.0077384233529271E-4 0.0 0.0 8.9918484038935 0.0 66-67 1.0077384233529271E-4 0.0 0.0 9.83250379665451 0.0 68-69 1.0077384233529271E-4 0.0 0.0 10.741635015282352 0.0 70-71 1.0077384233529271E-4 0.0 0.0 11.720350572042715 0.0 72-73 1.0077384233529271E-4 0.0 0.0 12.673923055140424 0.0 74-75 1.0077384233529271E-4 0.0 0.0 13.662464061528476 0.0 76-77 1.0077384233529271E-4 0.0 0.0 14.681489155222957 0.0 78-79 1.0077384233529271E-4 0.0 0.0 15.783400734238215 0.0 80-81 1.0077384233529271E-4 0.0 0.0 16.94814480394953 0.0 82-83 1.0077384233529271E-4 0.0 0.0 18.06199808328152 0.0 84-85 1.0077384233529271E-4 0.0 0.0 19.236063733408848 0.0 86-87 1.0077384233529271E-4 0.0 0.0 20.50077545471677 0.0 88-89 1.0077384233529271E-4 0.0 0.0 21.746088211375152 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 325 0.0 32.116497 9 TAACGCA 75 3.0955393E-4 31.636263 4 ATGGGCG 85 6.4426434E-4 27.914347 5 CCTTTAG 385 0.0 27.178432 1 GGCATTC 405 0.0 26.943975 7 TTAGGCA 410 0.0 26.620756 4 CTTTAGG 390 0.0 25.552364 2 GATTGGC 520 0.0 23.722414 9 ATGCCGC 70 1.7964127E-5 23.72241 94-95 GTACATG 5085 0.0 22.8224 1 CATATAG 335 0.0 22.664783 3 GGTATGC 105 0.0022108934 22.592775 8 GATATGA 570 0.0 22.478395 2 GAGTACT 5380 0.0 22.26844 12-13 GGATATG 615 0.0 21.654362 1 CATGGGT 665 0.0 21.407995 4 GTACTTT 5575 0.0 21.191671 14-15 GTTCGCA 135 3.8253446E-4 21.08659 9 CATGGGG 1175 0.0 21.001093 4 ACATGGG 4975 0.0 20.889471 3 >>END_MODULE