FastQCFastQC Report
Thu 26 May 2016
SRR938757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5270108
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150350.2852882711322045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113700.2157451042749029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73060.13863093507761132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA224650.033.6771241
GGTATCA144000.031.7338711
GTACATG249700.028.4191931
TCAACGC276150.027.108274
ATCAACG276000.027.0886733
TACATGG254950.026.9651092
CAACGCA280150.026.7212185
TATCAAC284200.026.373772
AACGCAG287400.026.0935046
ACATGGG259000.025.665383
ACGCAGA324800.022.8719887
CGCAGAG326550.022.7059158
CATGGGG101750.021.8387724
GTATAGG15350.021.3083211
CATGGGA156850.021.0541674
GAGTACT154700.020.78197112-13
GCAGAGT346100.020.725849
TACCTGG37900.020.126882
AGTACTT162150.019.25773212-13
GTGTACG6450.019.1083051
Produced by FastQC (version 0.11.5)