FastQCFastQC Report
Thu 26 May 2016
SRR938757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5270108
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150190.2849846720408766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122070.2316271317399947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80190.15216006958491174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155700.033.322231
GTATCAA234950.031.2277071
GTACATG251850.026.1054951
ATCAACG284050.025.5765043
TCAACGC285100.025.4989534
CAACGCA289200.025.1374535
TACATGG258650.024.8041882
TATCAAC296550.024.5784262
AACGCAG297900.024.403336
ACATGGG258150.023.9147573
ACGCAGA338100.021.263187
CGCAGAG339600.021.0854248
CATGGGG101050.020.8031084
GAGTACT165100.020.46531712-13
CATGGGA156600.019.9689144
GTACTTT174750.019.25371714-15
GCAGAGT366900.019.1414499
AGTACTT171450.018.66938412-13
AGTACAT192700.018.074872
AGAGTAC325750.017.33580210-11
Produced by FastQC (version 0.11.5)