##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938757_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5270108 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00073793554136 33.0 31.0 34.0 30.0 34.0 2 32.22312047495042 34.0 31.0 34.0 30.0 34.0 3 32.31906082380095 34.0 31.0 34.0 30.0 34.0 4 35.95119303057926 37.0 35.0 37.0 35.0 37.0 5 35.859738548052526 37.0 35.0 37.0 35.0 37.0 6 35.88640858972909 37.0 35.0 37.0 35.0 37.0 7 35.81558404495696 37.0 35.0 37.0 33.0 37.0 8 35.779411541471255 37.0 35.0 37.0 33.0 37.0 9 37.43151278873222 39.0 37.0 39.0 34.0 39.0 10-11 37.356406737774634 39.0 37.0 39.0 34.0 39.0 12-13 37.289929257616734 39.0 37.0 39.0 33.5 39.0 14-15 38.524242672066684 40.0 38.0 41.0 34.0 41.0 16-17 38.45822239316537 40.0 38.0 41.0 33.5 41.0 18-19 38.47411798771486 40.0 38.0 41.0 33.5 41.0 20-21 38.54093369623544 40.0 38.0 41.0 34.0 41.0 22-23 38.49933378974397 40.0 38.0 41.0 34.0 41.0 24-25 38.43628593569619 40.0 38.0 41.0 34.0 41.0 26-27 38.28776924495665 40.0 38.0 41.0 33.5 41.0 28-29 38.13458234632004 40.0 38.0 41.0 33.0 41.0 30-31 37.92546993344349 40.0 37.0 41.0 33.0 41.0 32-33 37.7339730039688 40.0 37.0 41.0 33.0 41.0 34-35 37.718122380034714 40.0 37.0 41.0 33.0 41.0 36-37 37.648789265798726 40.0 37.0 41.0 32.5 41.0 38-39 37.56300610917272 40.0 37.0 41.0 32.0 41.0 40-41 37.4151964248171 40.0 37.0 41.0 32.0 41.0 42-43 37.255141355736924 40.0 36.0 41.0 31.0 41.0 44-45 37.02577347560999 40.0 36.0 41.0 31.0 41.0 46-47 36.7634759097916 40.0 36.0 41.0 30.5 41.0 48-49 36.52085621395236 39.0 36.0 41.0 30.0 41.0 50-51 36.30754635388877 39.0 35.0 41.0 30.0 41.0 52-53 36.11090028895043 39.0 35.0 41.0 29.0 41.0 54-55 35.878444331691114 39.0 35.0 40.0 28.5 41.0 56-57 35.637620803975935 38.5 35.0 40.0 28.0 41.0 58-59 35.353797683083535 38.0 34.5 40.0 28.0 41.0 60-61 35.031200688866335 38.0 34.0 40.0 27.0 41.0 62-63 34.67806712879508 37.5 34.0 40.0 26.5 41.0 64-65 34.32134739933223 37.0 34.0 40.0 26.0 41.0 66-67 33.94028794096819 36.0 33.0 39.0 26.0 41.0 68-69 33.506400722717636 36.0 33.0 39.0 25.5 40.0 70-71 33.043692368353746 35.0 32.0 38.5 24.5 40.0 72-73 32.70208599140663 35.0 32.0 38.0 24.0 40.0 74-75 32.27623124611488 35.0 32.0 37.0 22.5 39.0 76-77 30.21035868714645 33.0 29.5 35.5 20.0 37.5 78-79 31.28508163400067 34.0 31.0 36.0 21.0 38.0 80-81 31.340874323638147 35.0 31.0 36.0 21.0 37.5 82-83 31.04163557938471 34.0 31.0 35.0 20.0 37.0 84-85 30.662920949627598 34.0 31.0 35.0 19.0 36.5 86-87 30.136922146567017 34.0 30.0 35.0 16.5 36.0 88-89 29.638274490769447 34.0 30.0 35.0 8.5 36.0 90-91 29.239829904814094 34.0 29.0 35.0 4.5 35.0 92-93 28.972695815721423 34.0 29.0 35.0 2.0 35.0 94-95 28.599987609362085 34.0 29.0 35.0 2.0 35.0 96-97 28.108217706354402 34.0 29.0 35.0 2.0 35.0 98-99 27.40427700912391 33.0 27.5 35.0 2.0 35.0 100-101 24.307035833041752 30.0 20.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08702921812367492 1101 2 0.12142609422112827 1101 3 0.1317600708070259 1101 4 0.08352527059344084 1101 5 0.047785757892306435 1101 6 0.1344126380070989 1101 7 0.15580260162002446 1101 8 0.14680135702560904 1101 9 0.20130399530053467 1101 10-11 0.1425319249857253 1101 12-13 0.22048347748014407 1101 14-15 0.2635277553126585 1101 16-17 0.21656337512172996 1101 18-19 0.19670759283748396 1101 20-21 0.17919001724732908 1101 22-23 0.15672513488467388 1101 24-25 0.21425992201204735 1101 26-27 0.26646186482528833 1101 28-29 0.288502722193428 1101 30-31 0.19512913734702408 1101 32-33 0.22473716692625345 1101 34-35 0.24539422658860843 1101 36-37 0.23124178167058318 1101 38-39 0.1912897653096408 1101 40-41 0.2628461417515453 1101 42-43 0.21600069681132084 1101 44-45 0.26081522567785953 1101 46-47 0.25114465951453724 1101 48-49 0.12730849102775466 1101 50-51 0.22150284830028255 1101 52-53 0.2145673874258449 1101 54-55 0.16505094169139767 1101 56-57 -0.02199979327239987 1101 58-59 0.14394376254335128 1101 60-61 0.24749856472529075 1101 62-63 0.035964100841049174 1101 64-65 0.07587604226323208 1101 66-67 0.035548957706858175 1101 68-69 0.05344793730698427 1101 70-71 0.038846475744144016 1101 72-73 0.09802480007834191 1101 74-75 0.09354868711555753 1101 76-77 0.03172115344712623 1101 78-79 0.07523530522633592 1101 80-81 0.17717177291870456 1101 82-83 0.15240499860698975 1101 84-85 0.20317954873804567 1101 86-87 0.23578004080350468 1101 88-89 0.22660842438674678 1101 90-91 0.3293978441895007 1101 92-93 0.3646355297846888 1101 94-95 0.38512542539246297 1101 96-97 0.25702740616934605 1101 98-99 0.3847869148859999 1101 100-101 0.9719426946225944 1102 1 0.09195625777786631 1102 2 0.09338660222355344 1102 3 0.08685243462630865 1102 4 0.0736433209786469 1102 5 0.07431757205476686 1102 6 0.11682651243475561 1102 7 0.10734380501680363 1102 8 0.048982850500159714 1102 9 0.12389399269288504 1102 10-11 0.1936938391923846 1102 12-13 0.17863702436371653 1102 14-15 0.22964078602154814 1102 16-17 0.2615732024259003 1102 18-19 0.22155429199055732 1102 20-21 0.21992343304604844 1102 22-23 0.16653370779847876 1102 24-25 0.06157171200135991 1102 26-27 0.18087838504381892 1102 28-29 0.16473369377005298 1102 30-31 0.12814929479159787 1102 32-33 0.15269229271178375 1102 34-35 0.14508516075181177 1102 36-37 0.12023440793524998 1102 38-39 0.14649140112290837 1102 40-41 0.22103615039775804 1102 42-43 0.2595975033575968 1102 44-45 0.2046222302260432 1102 46-47 0.15214410078664287 1102 48-49 0.12182266683614529 1102 50-51 0.17916602129690062 1102 52-53 0.15052937278109368 1102 54-55 0.17496763477758037 1102 56-57 0.11678721395329461 1102 58-59 -0.009492130748121497 1102 60-61 -0.06793004792068302 1102 62-63 -0.15202961285650218 1102 64-65 -0.04401493498942699 1102 66-67 -0.009568668770235433 1102 68-69 0.027580138859150338 1102 70-71 0.043348795342915025 1102 72-73 -0.04882824391236795 1102 74-75 0.019061913331995584 1102 76-77 -0.07836378112279618 1102 78-79 -0.06268381386050237 1102 80-81 -0.06695307774573322 1102 82-83 -0.0068040700432767665 1102 84-85 0.1155323634029628 1102 86-87 -0.03652267150978972 1102 88-89 0.1962904593118644 1102 90-91 0.2188737445882758 1102 92-93 0.33258783077493703 1102 94-95 0.30081136631458705 1102 96-97 0.2377372643838811 1102 98-99 0.37779564097318996 1102 100-101 0.7743546279476092 1103 1 -0.035803905781573775 1103 2 -0.00408510293663511 1103 3 -0.004725332318649578 1103 4 0.012801006259550718 1103 5 -0.008855703535026294 1103 6 -0.002313237125569856 1103 7 0.024710317381050118 1103 8 0.034559949193074146 1103 9 -0.056393504930831284 1103 10-11 -0.05210468934721035 1103 12-13 0.0069660603252614806 1103 14-15 0.017185795557480787 1103 16-17 -0.06900912269941983 1103 18-19 -0.004426216765281765 1103 20-21 0.009129278367154825 1103 22-23 -0.02608520403273218 1103 24-25 0.01635257775527066 1103 26-27 -0.05914672198004922 1103 28-29 -0.0300730812086627 1103 30-31 -0.07989060556386107 1103 32-33 0.023063318109279862 1103 34-35 -0.08404844544177337 1103 36-37 -0.09323088931844126 1103 38-39 0.039459251707569365 1103 40-41 -0.013425264016824201 1103 42-43 -0.006355297750857858 1103 44-45 0.039135283994212955 1103 46-47 0.024071750470341158 1103 48-49 0.11487111063161137 1103 50-51 0.10312449862996687 1103 52-53 0.12961989332181645 1103 54-55 0.06384334642776679 1103 56-57 0.05454097296429694 1103 58-59 -0.22855559659571867 1103 60-61 -0.1239238601530559 1103 62-63 -0.07829531337474549 1103 64-65 -0.11972136153310231 1103 66-67 -0.20783483269943304 1103 68-69 -0.14192219619854285 1103 70-71 -0.19392343788936017 1103 72-73 -0.08228545199564508 1103 74-75 -0.04624987325718166 1103 76-77 -0.13651801376815698 1103 78-79 -0.1916711023336397 1103 80-81 -0.06614716429351475 1103 82-83 -0.035447979202043456 1103 84-85 -0.11725127572750438 1103 86-87 0.03366002322495376 1103 88-89 0.2013411350915355 1103 90-91 0.2395893152898836 1103 92-93 0.1371269262243473 1103 94-95 0.06931410303242558 1103 96-97 0.08342292511668603 1103 98-99 0.17403473640395006 1103 100-101 0.6896690916492254 1104 1 -0.021400614059459144 1104 2 -0.052681402730726745 1104 3 -0.035914378529064095 1104 4 -0.030622813594789022 1104 5 0.02879406332289136 1104 6 -0.010613510335353737 1104 7 -0.07418101418243594 1104 8 -0.0613110633128926 1104 9 -0.028065852749058706 1104 10-11 0.012650642689735037 1104 12-13 -0.014784177486056649 1104 14-15 -0.020960709601212102 1104 16-17 0.05943687379508589 1104 18-19 0.053886052960720576 1104 20-21 0.006101189641562144 1104 22-23 -0.09766455375687855 1104 24-25 -0.0448486330043707 1104 26-27 -6.693932766950184E-4 1104 28-29 -0.06249334099364745 1104 30-31 -0.1362349930902127 1104 32-33 -0.1755469076066447 1104 34-35 -0.13023261646429063 1104 36-37 -0.12831670288596086 1104 38-39 0.019674702744602257 1104 40-41 0.001387682525333389 1104 42-43 0.022484111611611013 1104 44-45 -0.06408767469836363 1104 46-47 -0.10108522284765087 1104 48-49 -0.04757564111924495 1104 50-51 0.06878355087321353 1104 52-53 0.011069833090161296 1104 54-55 -0.07643600325176436 1104 56-57 -0.05856749427869801 1104 58-59 -0.10138728298247912 1104 60-61 -0.08089833802020507 1104 62-63 -0.23537919114247075 1104 64-65 -0.06391304392870012 1104 66-67 -0.016487406452178277 1104 68-69 -0.019403025193128087 1104 70-71 -0.020703576654888423 1104 72-73 -0.16345922653311362 1104 74-75 -0.16662654899614182 1104 76-77 -0.19199770043566033 1104 78-79 -0.03337378085421605 1104 80-81 -0.08002775066627876 1104 82-83 -0.10751810056797595 1104 84-85 -0.11604534145577361 1104 86-87 0.127561294759019 1104 88-89 0.18376965897802222 1104 90-91 0.2270405764222545 1104 92-93 0.25598702967022646 1104 94-95 0.22986927518521938 1104 96-97 0.3323863993494207 1104 98-99 0.3260753560799614 1104 100-101 0.8552365361600422 1105 1 -0.0523939167947951 1105 2 -0.05437932197974504 1105 3 -0.014816484818290121 1105 4 -0.05659404744811525 1105 5 -0.05779292803618574 1105 6 -0.025573649713756197 1105 7 -0.011651940975788477 1105 8 -0.007616249994946145 1105 9 0.0653463143906734 1105 10-11 0.10071141564230146 1105 12-13 -0.02356950862483842 1105 14-15 -6.969136362329209E-4 1105 16-17 -0.00744927710367449 1105 18-19 -0.05774181131364742 1105 20-21 0.054470795732505906 1105 22-23 0.025709454089032135 1105 24-25 0.007699726559415865 1105 26-27 0.014120411745722095 1105 28-29 0.07312894401049164 1105 30-31 -0.05698113856759335 1105 32-33 0.06345456912533365 1105 34-35 0.11520858012108448 1105 36-37 0.05583095389238224 1105 38-39 0.022498129844727544 1105 40-41 -0.010951726720300314 1105 42-43 0.017014317179807392 1105 44-45 0.08878107838304317 1105 46-47 0.11717302947164399 1105 48-49 0.06092111301712322 1105 50-51 -0.022204847258890936 1105 52-53 -0.04440072734230682 1105 54-55 -0.024056255458766884 1105 56-57 -0.07087314818451063 1105 58-59 0.02321415844750163 1105 60-61 -0.06543795256867924 1105 62-63 -0.09667831652365777 1105 64-65 -0.07547428447151816 1105 66-67 -0.07021390434262997 1105 68-69 -0.023927358578312408 1105 70-71 -0.02447151188216168 1105 72-73 -0.11687685756650268 1105 74-75 -0.055566111617835645 1105 76-77 -0.06390694277232001 1105 78-79 -0.11182496564832078 1105 80-81 -0.013532021871263566 1105 82-83 -0.03816134100436486 1105 84-85 0.008782894809030495 1105 86-87 -0.04375746369539257 1105 88-89 0.022186860464636027 1105 90-91 0.13114284711208057 1105 92-93 0.028141069689620934 1105 94-95 0.12868117518496902 1105 96-97 0.20737234378717773 1105 98-99 0.3662078895864411 1105 100-101 0.7368986527583878 1106 1 -0.023439860234265808 1106 2 -0.11684994200702903 1106 3 -0.06489892196748315 1106 4 -0.0663993819941524 1106 5 -0.057882239506383826 1106 6 -0.11557667295625862 1106 7 -0.09070874766587167 1106 8 -0.07010368202213613 1106 9 -0.11464839037638086 1106 10-11 -0.10242869031340973 1106 12-13 -0.021739115811186593 1106 14-15 -0.009489316071011444 1106 16-17 -0.13794159518671023 1106 18-19 -0.2627825120348817 1106 20-21 -0.19410890025839933 1106 22-23 -0.17371937553685513 1106 24-25 -0.1920110231646106 1106 26-27 -0.09368495124578402 1106 28-29 -0.16952742126430564 1106 30-31 -0.2239943354404872 1106 32-33 -0.2545036007225434 1106 34-35 -0.18917484798792117 1106 36-37 -0.22076588523282936 1106 38-39 -0.1705352107944762 1106 40-41 -0.08550785892462187 1106 42-43 -0.047519474392977656 1106 44-45 0.0848276691392229 1106 46-47 0.020562707924824508 1106 48-49 0.002659984366665924 1106 50-51 -0.001681990415072221 1106 52-53 -0.061885189006389396 1106 54-55 -0.025145115083667235 1106 56-57 -0.0022282391388799283 1106 58-59 -0.06569125271894904 1106 60-61 -0.04073787901161552 1106 62-63 -0.05323021910596282 1106 64-65 -0.047292978175477174 1106 66-67 -0.15844891422013774 1106 68-69 -0.12258608190931142 1106 70-71 -0.12258442871308972 1106 72-73 -0.061199014870659596 1106 74-75 0.03621473502865058 1106 76-77 -0.05020932026626568 1106 78-79 -0.04415383972935771 1106 80-81 -0.09106827251812533 1106 82-83 0.06248030483572009 1106 84-85 -0.15126211102411702 1106 86-87 -0.010325799004714753 1106 88-89 0.1820897848409082 1106 90-91 0.1900371608998519 1106 92-93 0.14462994003627827 1106 94-95 0.2503563104559241 1106 96-97 0.18411378069896145 1106 98-99 0.2628076534890731 1106 100-101 0.6903295224347588 1107 1 -0.022803565271285464 1107 2 -0.033342783598897086 1107 3 -0.05408740342659968 1107 4 -0.018478583726668774 1107 5 -0.032021342910240946 1107 6 -0.06529582886863494 1107 7 -0.02213810523928572 1107 8 0.002770338831126651 1107 9 -0.05634931019150713 1107 10-11 -9.978528171998846E-4 1107 12-13 0.01644788536592756 1107 14-15 -0.008713775459163742 1107 16-17 0.027384837348030544 1107 18-19 0.08096003671546015 1107 20-21 0.010926016221155521 1107 22-23 -0.09283239889341388 1107 24-25 -0.10069811611849389 1107 26-27 -0.02161328562500131 1107 28-29 -0.030081749338044972 1107 30-31 -0.07368193523200262 1107 32-33 0.024641844437873317 1107 34-35 0.014015301617185116 1107 36-37 0.07361470062263464 1107 38-39 0.034082830829440525 1107 40-41 -0.010911470151775404 1107 42-43 -0.06527822470592781 1107 44-45 -0.04727426305804272 1107 46-47 -0.018121432367550483 1107 48-49 -0.23683671256777217 1107 50-51 -0.14950095418893028 1107 52-53 -0.05106991677781991 1107 54-55 -0.03938048171837494 1107 56-57 -0.07833756161083016 1107 58-59 -0.04722003811541953 1107 60-61 -0.11043875859583352 1107 62-63 -0.14253423871278414 1107 64-65 -0.09817777085518031 1107 66-67 -0.18818485576174737 1107 68-69 -0.062174878893721086 1107 70-71 -0.1830800986816925 1107 72-73 -0.1689817923768402 1107 74-75 -0.11447023215262675 1107 76-77 -0.2058683187536552 1107 78-79 -0.1619644053005338 1107 80-81 -0.0982779391439621 1107 82-83 -0.06863092321642839 1107 84-85 -0.08395933498348995 1107 86-87 -0.06908020689370886 1107 88-89 0.1445602537357651 1107 90-91 0.2181472707481973 1107 92-93 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-2.3557183133575386 2316 98-99 -2.686489773937108 2316 100-101 -3.3857516079617795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 21.0 4 55.0 5 125.0 6 314.0 7 737.0 8 1729.0 9 3044.0 10 5134.0 11 7623.0 12 11121.0 13 15411.0 14 18694.0 15 24121.0 16 30681.0 17 40569.0 18 37621.0 19 26922.0 20 25011.0 21 26474.0 22 28577.0 23 32558.0 24 38674.0 25 46981.0 26 57707.0 27 71369.0 28 87907.0 29 108470.0 30 133061.0 31 161891.0 32 199697.0 33 251934.0 34 331823.0 35 460581.0 36 693611.0 37 1122279.0 38 1050430.0 39 117138.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63281225741548 19.886506399992836 14.002528641522868 23.478152701068815 2 15.860206280402602 21.215428602222193 39.082121277211016 23.842243840164187 3 19.213913642756467 25.170774488871956 29.85468229493589 25.76062957343569 4 12.99299748695852 17.008228294372714 36.928370348387546 33.07040387028122 5 13.478604233537528 37.9974565986124 34.223207569939746 14.300731597910326 6 31.86946073970401 36.39016505923598 17.5073072506294 14.233066950430617 7 28.71049321949379 32.726900473386884 20.86257055832632 17.700035748793002 8 26.38735297265255 35.221175733021035 20.032967066329572 18.358504227996846 9 26.77700343142873 16.400536763193468 20.411365383783405 36.4110944215944 10-11 24.947164270637337 26.844706028794857 27.489284849570446 20.71884485099736 12-13 25.80202341204393 24.111925220507814 27.99590634575231 22.09014502169595 14-15 22.660669687736355 25.446300998577254 26.231912575439775 25.661116738246616 16-17 22.104438927671385 28.844177853122655 26.801830136647343 22.249553082558617 18-19 21.931877769693386 27.346129357495176 29.214034912616942 21.507957960194496 20-21 22.809155221217026 26.56958136053202 29.01345940286797 21.60780401538299 22-23 22.41130542296287 26.691805936424835 28.766573664145024 22.130314976467275 24-25 22.274334795415957 26.780580208223437 29.084081388844403 21.861003607516203 26-27 22.129039297167036 27.055928730005125 28.92279083888351 21.892241133944328 28-29 21.900537901690058 27.0206796521058 29.183576503555525 21.895205942648612 30-31 21.943117674248803 26.915586929148322 29.073237208801032 22.068058187801846 32-33 21.849457354574138 26.981173820346754 28.86365896106873 22.305709864010375 34-35 22.38257889265119 26.926652897953034 28.77338865108703 21.91737955830875 36-37 22.37999993167742 26.640273275130088 28.744504302614367 22.235222490578128 38-39 22.145332528316267 26.96107860141474 28.94596185353946 21.947627016729534 40-41 22.184198528654797 26.776575561930617 29.00560614118443 22.03361976823016 42-43 22.195294676201822 26.833036585303372 28.703099468813754 22.268569269681056 44-45 22.11135573465098 27.15652411929596 28.443829011466313 22.28829113458675 46-47 22.190362718270336 27.15619487086654 28.129190573154183 22.52425183770894 48-49 22.33676346706911 27.22902795778946 27.980796589366193 22.453411985775237 50-51 22.265466380370984 27.390458885222905 27.84391534391534 22.50015939049076 52-53 22.526284091331714 27.319857581666255 27.815113086866532 22.338745240135495 54-55 22.08083781205243 27.161151156674592 28.05662616401789 22.701384867255094 56-57 22.185636423390186 27.612014782239758 27.762599931538407 22.439748862831653 58-59 22.323916843023856 27.0586068790973 27.86770260267347 22.749773675205372 60-61 22.30681938177534 27.174473529738524 28.09627264282507 22.422434445661064 62-63 22.27106161771258 27.08888508546694 28.06654057184407 22.573512724976414 64-65 22.035601547444568 26.981705118756576 27.96415177829373 23.018541555505124 66-67 21.982619701245575 27.39937353505827 27.687406992062215 22.930599771633936 68-69 22.10131823725994 27.14515258626644 27.85969744602916 22.893831730444465 70-71 21.936383606139607 27.155007974843308 27.848858716478595 23.059749702538486 72-73 22.04241355205624 27.10078237485835 27.716737493804683 23.140066579280727 74-75 21.87671486049242 27.26714518943445 27.60229012384566 23.253849826227473 76-77 21.97059558145476 27.037496759170004 27.848991092822118 23.142916566553115 78-79 21.880957221231707 27.05707600434372 27.723364180148742 23.33860259427583 80-81 21.7156876929813 27.24911896874871 27.705647091304204 23.329546246965783 82-83 21.93404765139538 27.17266894720184 27.52528031683601 23.36800308456677 84-85 21.62539173770253 26.96326147395841 27.573040248890536 23.838306539448528 86-87 21.795781794464816 26.771087987034708 27.800658602886173 23.6324716156143 88-89 21.85225533940151 26.68471414123386 27.622326134808734 23.84070438455589 90-91 21.571774458415298 26.907691505560372 27.776712171845702 23.74382186417863 92-93 21.70130099800611 26.76789545868889 27.68695632043973 23.84384722286526 94-95 21.547189287499965 26.461351339299693 27.66440137340717 24.327057999793173 96-97 21.603516438477033 26.369171012721758 27.636688453076363 24.390624095724846 98-99 21.35069948401349 26.37523507622912 27.585117471227772 24.68894796852962 100-101 21.433003464279704 26.10850973355008 28.082581809618883 24.375904992551334 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1205.0 1 810.0 2 565.5 3 770.0 4 684.5 5 665.0 6 1199.5 7 2425.0 8 3779.0 9 4351.5 10 3966.0 11 3554.0 12 3756.5 13 4211.0 14 4629.0 15 5059.5 16 5446.5 17 5784.5 18 6246.0 19 6783.0 20 7575.5 21 8935.5 22 10613.0 23 13007.5 24 15762.0 25 19550.0 26 26096.0 27 34027.5 28 40478.0 29 47760.5 30 57352.0 31 67920.0 32 80829.5 33 93121.5 34 106512.5 35 118139.0 36 130163.5 37 144245.0 38 151860.0 39 153638.0 40 154364.0 41 161109.5 42 171760.0 43 182315.5 44 191047.0 45 197456.0 46 198773.0 47 200163.5 48 219459.5 49 224863.0 50 209499.5 51 198699.5 52 188294.5 53 183670.5 54 177485.0 55 162392.0 56 145456.0 57 128840.5 58 115261.5 59 101336.0 60 85389.5 61 69048.0 62 54037.5 63 41061.0 64 29561.5 65 22051.0 66 15799.5 67 11998.5 68 9098.5 69 6263.0 70 4735.0 71 3720.0 72 3013.0 73 2571.5 74 2077.0 75 1723.0 76 1213.0 77 621.5 78 364.5 79 228.0 80 144.5 81 104.0 82 66.5 83 36.0 84 19.5 85 13.5 86 13.5 87 7.5 88 1.5 89 1.5 90 2.0 91 1.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3918136023018883 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.897494320799498E-4 16-17 0.0022580182417514024 18-19 1.4231207405996233E-4 20-21 9.48747160399749E-6 22-23 0.0 24-25 0.0 26-27 1.5179954566395983E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008187687994249833 36-37 0.01870929400308305 38-39 0.012419100329632714 40-41 0.008358462483121788 42-43 0.003889863357638971 44-45 0.0020018565084434703 46-47 0.0018026196047595227 48-49 4.743735801998745E-5 50-51 6.071981826558393E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.123234666158644E-4 60-61 6.356605974678317E-4 62-63 0.0 64-65 0.0 66-67 0.0011479840640836961 68-69 7.589977283197992E-4 70-71 0.0018690319059875052 72-73 0.0 74-75 0.0 76-77 0.09967537667159762 78-79 0.03447747180892688 80-81 1.0436218764397238E-4 82-83 0.0 84-85 0.0 86-87 0.15293804225643953 88-89 0.32818113025387713 90-91 0.2823851048213813 92-93 0.0 94-95 5.692482962398494E-5 96-97 5.692482962398494E-5 98-99 1.707744888719548E-4 100-101 7.020728986958141E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5270108.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.86223724378477 #Duplication Level Percentage of deduplicated Percentage of total 1 63.57075538441161 22.797895113623774 2 15.71908656191667 11.274432230780892 3 7.06100073975363 7.596698511227533 4 3.839500543800571 5.507723175976668 5 2.4057062477753357 4.313700409828717 6 1.5929916098020926 3.4276945822848752 7 1.127573921711022 2.830612644321383 8 0.826061113209891 2.3699519695838442 9 0.6237728128103722 2.0132899739305663 >10 2.884806534361143 17.84100373350666 >50 0.19273497225858072 4.838689816902711 >100 0.14156136447085613 9.90644284924822 >500 0.010637683922218952 2.5359666257571125 >1k 0.003651738554592622 2.076960959416537 >5k 5.292374716787782E-5 0.15219773129674657 >10k+ 1.0584749433575564E-4 0.5167396723139073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15019 0.2849846720408766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12207 0.2316271317399947 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8019 0.15216006958491174 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.897494320799498E-5 0.0 5 1.897494320799498E-5 0.0 0.0 1.897494320799498E-5 0.0 6 1.897494320799498E-5 0.0 0.0 1.897494320799498E-5 0.0 7 1.897494320799498E-5 0.0 0.0 1.897494320799498E-5 0.0 8 1.897494320799498E-5 0.0 0.0 1.897494320799498E-5 0.0 9 1.897494320799498E-5 0.0 0.0 3.794988641598996E-5 0.0 10-11 1.897494320799498E-5 0.0 0.0 5.6924829623984934E-5 0.0 12-13 1.897494320799498E-5 0.0 0.0 5.6924829623984934E-5 0.0 14-15 3.794988641598996E-5 0.0 0.0 9.48747160399749E-5 0.0 16-17 3.794988641598996E-5 0.0 0.0 1.1384965924796987E-4 0.0 18-19 3.794988641598996E-5 0.0 0.0 1.2333713085196734E-4 0.0 20-21 3.794988641598996E-5 0.0 0.0 2.0872437528794474E-4 0.0 22-23 3.794988641598996E-5 0.0 0.0 4.933485234078695E-4 0.0 24-25 5.6924829623984934E-5 0.0 0.0 8.728473875677691E-4 0.0 26-27 5.6924829623984934E-5 0.0 0.0 0.0016508200590955632 0.0 28-29 9.48747160399749E-5 0.0 0.0 0.004999897535306677 0.0 30-31 1.1384965924796987E-4 0.0 0.0 0.016299476215667685 0.0 32-33 1.1384965924796987E-4 0.0 0.0 0.03905992059365766 0.0 34-35 1.1384965924796987E-4 0.0 0.0 0.07522616234809609 0.0 36-37 1.1384965924796987E-4 0.0 1.897494320799498E-5 0.13891555922573123 0.0 38-39 1.1384965924796987E-4 0.0 1.897494320799498E-5 0.23225330486585855 0.0 40-41 1.1384965924796987E-4 0.0 1.897494320799498E-5 0.3348887726779034 0.0 42-43 1.3282460245596486E-4 0.0 1.897494320799498E-5 0.4413192291315472 0.0 44-45 1.5179954566395983E-4 0.0 1.897494320799498E-5 0.5699978064965652 0.0 46-47 2.0872437528794477E-4 0.0 1.897494320799498E-5 0.7285524319425712 0.0 48-49 2.2769931849593973E-4 0.0 1.897494320799498E-5 0.9002756679749258 0.0 50-51 2.2769931849593973E-4 0.0 1.897494320799498E-5 1.0840100430579411 0.0 52-53 2.2769931849593973E-4 0.0 1.897494320799498E-5 1.262383996684698 0.0 54-55 2.2769931849593973E-4 0.0 1.897494320799498E-5 1.4630819709956606 0.0 56-57 2.4667426170393473E-4 0.0 1.897494320799498E-5 1.7008380093918378 0.0 58-59 2.4667426170393473E-4 0.0 1.897494320799498E-5 1.96556692955818 0.0 60-61 2.4667426170393473E-4 0.0 1.897494320799498E-5 2.255845610754087 0.0 62-63 2.4667426170393473E-4 0.0 1.897494320799498E-5 2.5454032441080905 9.48747160399749E-6 64-65 2.4667426170393473E-4 0.0 1.897494320799498E-5 2.845131447021579 1.897494320799498E-5 66-67 2.4667426170393473E-4 0.0 1.897494320799498E-5 3.185731677605089 1.897494320799498E-5 68-69 2.4667426170393473E-4 0.0 1.897494320799498E-5 3.568209607848644 1.897494320799498E-5 70-71 2.9411161972392214E-4 0.0 1.897494320799498E-5 3.991882139796756 1.897494320799498E-5 72-73 3.130865629319172E-4 9.48747160399749E-6 1.897494320799498E-5 4.4184768129988985 1.897494320799498E-5 74-75 3.2257403453591466E-4 1.897494320799498E-5 1.897494320799498E-5 4.846077158191065 1.897494320799498E-5 76-77 3.2257403453591466E-4 1.897494320799498E-5 1.897494320799498E-5 5.310858904599298 1.897494320799498E-5 78-79 3.2257403453591466E-4 1.897494320799498E-5 1.897494320799498E-5 5.826038100167967 1.897494320799498E-5 80-81 3.794988641598996E-4 1.897494320799498E-5 1.897494320799498E-5 6.385931749406273 1.897494320799498E-5 82-83 4.1744875057588953E-4 1.897494320799498E-5 1.897494320799498E-5 6.957162927211359 1.897494320799498E-5 84-85 4.3642369378388453E-4 1.897494320799498E-5 1.897494320799498E-5 7.531705232606239 1.897494320799498E-5 86-87 4.5539863699187947E-4 1.897494320799498E-5 1.897494320799498E-5 8.130421615648105 1.897494320799498E-5 88-89 4.5539863699187947E-4 1.897494320799498E-5 1.897494320799498E-5 8.769393340705731 1.897494320799498E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15570 0.0 33.32223 1 GTATCAA 23495 0.0 31.227707 1 GTACATG 25185 0.0 26.105495 1 ATCAACG 28405 0.0 25.576504 3 TCAACGC 28510 0.0 25.498953 4 CAACGCA 28920 0.0 25.137453 5 TACATGG 25865 0.0 24.804188 2 TATCAAC 29655 0.0 24.578426 2 AACGCAG 29790 0.0 24.40333 6 ACATGGG 25815 0.0 23.914757 3 ACGCAGA 33810 0.0 21.26318 7 CGCAGAG 33960 0.0 21.085424 8 CATGGGG 10105 0.0 20.803108 4 GAGTACT 16510 0.0 20.465317 12-13 CATGGGA 15660 0.0 19.968914 4 GTACTTT 17475 0.0 19.253717 14-15 GCAGAGT 36690 0.0 19.141449 9 AGTACTT 17145 0.0 18.669384 12-13 AGTACAT 19270 0.0 18.07487 2 AGAGTAC 32575 0.0 17.335802 10-11 >>END_MODULE