FastQCFastQC Report
Thu 26 May 2016
SRR938756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273077
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151820.2879153860260337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113270.21480816608594944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75530.14323705115627935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA229250.032.1340521
GGTATCA151150.031.9154571
GTACATG256200.028.1416531
TACATGG261250.027.131182
ATCAACG279900.026.1539573
TCAACGC280200.026.1090224
ACATGGG263150.025.8896053
CAACGCA284600.025.7220385
TATCAAC290350.025.2453292
AACGCAG291200.025.153436
CATGGGA158550.022.2628654
ACGCAGA328250.022.257377
CGCAGAG331150.021.9189828
CATGGGG106250.021.8350564
GCAGAGT350700.020.1279839
GAGTACT152000.020.01749812-13
AGTACTT157600.019.30621712-13
GTACTTT165100.018.58785214-15
AGTACAT189850.018.0926572
AGAGTAC312400.017.79866410-11
Produced by FastQC (version 0.11.5)