##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938756_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5273077 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.364141088779853 33.0 31.0 34.0 28.0 34.0 2 31.42688547882005 33.0 31.0 34.0 28.0 34.0 3 31.447217440594933 33.0 31.0 34.0 28.0 34.0 4 34.9978871918616 37.0 35.0 37.0 32.0 37.0 5 35.033551378066356 37.0 35.0 37.0 32.0 37.0 6 35.080560742807286 37.0 35.0 37.0 32.0 37.0 7 34.98099686388043 37.0 35.0 37.0 32.0 37.0 8 34.939972050474516 37.0 35.0 37.0 32.0 37.0 9 36.48097628765899 39.0 37.0 39.0 32.0 39.0 10-11 36.35414445872875 39.0 37.0 39.0 32.0 39.0 12-13 36.239011396950964 39.0 37.0 39.0 32.0 39.0 14-15 37.36583895892284 40.0 37.0 41.0 32.0 41.0 16-17 37.24510944937842 40.0 37.0 41.0 31.0 41.0 18-19 37.177011448913035 40.0 37.0 41.0 31.0 41.0 20-21 37.06454409825611 40.0 36.5 41.0 31.0 41.0 22-23 37.07774426582429 40.0 37.0 41.0 31.0 41.0 24-25 37.01144919749892 40.0 37.0 41.0 30.0 41.0 26-27 36.88136888575684 40.0 36.0 41.0 30.0 41.0 28-29 36.706594081596 39.5 36.0 41.0 30.0 41.0 30-31 36.4856042306987 39.0 36.0 41.0 30.0 41.0 32-33 36.3201841164087 39.0 35.5 41.0 29.5 41.0 34-35 36.03415045902042 39.0 35.0 41.0 28.5 41.0 36-37 35.827051548839506 39.0 35.0 41.0 27.0 41.0 38-39 35.662442725566116 39.0 35.0 40.0 27.0 41.0 40-41 35.560638029749995 39.0 35.0 40.5 27.0 41.0 42-43 35.571172012090855 39.0 35.0 41.0 26.5 41.0 44-45 35.40767885619725 39.0 35.0 41.0 26.0 41.0 46-47 35.12664313454933 39.0 35.0 41.0 24.5 41.0 48-49 34.86628917044071 39.0 34.0 40.5 24.0 41.0 50-51 33.747162235635855 38.0 32.5 39.5 22.5 40.5 52-53 33.8305711257393 38.0 33.0 39.5 23.0 40.5 54-55 34.33419794552593 38.0 34.0 40.0 23.0 41.0 56-57 34.24214476670832 38.0 34.0 40.0 22.5 41.0 58-59 34.03994859168566 38.0 33.5 40.0 22.0 41.0 60-61 33.74025052165936 38.0 33.0 40.0 20.5 41.0 62-63 33.34628007518191 37.0 33.0 40.0 19.5 41.0 64-65 32.95076736030974 36.5 32.0 39.5 18.0 41.0 66-67 32.56483728570624 36.0 32.0 39.0 15.5 41.0 68-69 32.13657983754078 35.5 31.0 39.0 12.5 40.5 70-71 31.708573855454794 35.0 31.0 38.5 9.0 40.0 72-73 31.27823403678725 35.0 31.0 37.5 7.5 40.0 74-75 30.79547776374212 35.0 30.0 37.0 4.5 39.0 76-77 30.32388034538468 35.0 30.0 36.5 2.0 39.0 78-79 29.877214290631446 34.0 29.5 36.0 2.0 38.5 80-81 29.391228878319055 34.0 29.0 35.5 2.0 37.0 82-83 28.972529132421165 34.0 29.0 35.0 2.0 37.0 84-85 28.610802762789163 34.0 29.0 35.0 2.0 36.5 86-87 28.166794833453032 34.0 27.5 35.0 2.0 36.0 88-89 27.702974468227943 34.0 26.5 35.0 2.0 36.0 90-91 27.2349242197677 33.5 26.0 35.0 2.0 35.0 92-93 26.750124452952235 33.0 25.0 35.0 2.0 35.0 94-95 26.209665248582564 33.0 24.5 35.0 2.0 35.0 96-97 25.580971603486923 33.0 21.5 35.0 2.0 35.0 98-99 24.776796451104353 32.0 10.0 35.0 2.0 35.0 100-101 22.34722686583185 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18597865719370077 1101 2 0.2780598100273579 1101 3 0.40965886142408436 1101 4 0.2551076852292624 1101 5 0.2033232873303632 1101 6 0.376854084264977 1101 7 0.39499392671454103 1101 8 0.4671683126957831 1101 9 0.5725586330668477 1101 10-11 0.4375305651023851 1101 12-13 0.4229717213418951 1101 14-15 0.520315004330449 1101 16-17 0.7139610629606281 1101 18-19 0.6818818426941462 1101 20-21 0.7100320166934111 1101 22-23 0.6507179803157399 1101 24-25 0.6691503294494012 1101 26-27 0.6092136377594883 1101 28-29 0.6757507272586238 1101 30-31 0.7333727636752982 1101 32-33 0.6483914129410024 1101 34-35 0.7143764584752788 1101 36-37 0.6840018265618824 1101 38-39 0.6664784748594172 1101 40-41 0.8159133929536964 1101 42-43 0.9090678836652728 1101 44-45 0.8893691729621693 1101 46-47 0.8418858034670578 1101 48-49 0.836904101538174 1101 50-51 0.9331230499561514 1101 52-53 0.910430660195189 1101 54-55 0.8592646539639972 1101 56-57 0.8253130563872446 1101 58-59 0.8525885494048566 1101 60-61 0.8418294775680977 1101 62-63 0.8523636365984615 1101 64-65 0.8280040651472405 1101 66-67 0.644827339709444 1101 68-69 0.738647548920369 1101 70-71 0.6231017865348996 1101 72-73 0.638491644165768 1101 74-75 0.4986659417669408 1101 76-77 0.46272241285436877 1101 78-79 0.42913084494439957 1101 80-81 0.3002220289132467 1101 82-83 0.3621887303003781 1101 84-85 0.31246416674602173 1101 86-87 0.17416814469472897 1101 88-89 0.20213967323860516 1101 90-91 0.09590695896165258 1101 92-93 0.05205297205793258 1101 94-95 0.09220669907664814 1101 96-97 0.05069428026684619 1101 98-99 -0.01138660816920023 1101 100-101 0.11560108825296922 1102 1 0.11055136188163317 1102 2 0.17985846293007413 1102 3 0.1703549035410532 1102 4 0.27709396313309753 1102 5 0.29734429200305357 1102 6 0.3126978855546767 1102 7 0.3035565147198156 1102 8 0.3535346478198633 1102 9 0.46106651206127935 1102 10-11 0.4257155891833193 1102 12-13 0.42258328736490824 1102 14-15 0.47101208681549167 1102 16-17 0.5914231388536919 1102 18-19 0.5960766785222731 1102 20-21 0.6348094945183291 1102 22-23 0.5898815602581564 1102 24-25 0.5148699323157899 1102 26-27 0.6288680920595269 1102 28-29 0.6796895249159931 1102 30-31 0.7178839790225169 1102 32-33 0.7331924066718827 1102 34-35 0.8017699956370521 1102 36-37 0.8469579068854713 1102 38-39 0.8607473920694417 1102 40-41 0.8742091086103372 1102 42-43 0.9319183666292403 1102 44-45 0.9112748402775708 1102 46-47 0.9030732760004341 1102 48-49 0.8325924933623128 1102 50-51 0.9088097018543735 1102 52-53 0.7908230583332951 1102 54-55 0.7052629140380802 1102 56-57 0.7257518944037287 1102 58-59 0.6221678640175412 1102 60-61 0.5814822098795958 1102 62-63 0.4951652043255237 1102 64-65 0.5885420789410816 1102 66-67 0.5581827061252866 1102 68-69 0.6443753671070809 1102 70-71 0.5985145166939212 1102 72-73 0.5549598097995379 1102 74-75 0.4718848047163853 1102 76-77 0.48841292128942015 1102 78-79 0.3839273005179926 1102 80-81 0.3816977013408014 1102 82-83 0.4164938630818398 1102 84-85 0.33713235211090975 1102 86-87 0.26443557310215837 1102 88-89 0.32980469265582 1102 90-91 0.22247544836514876 1102 92-93 0.16813711631035133 1102 94-95 0.24103502937557408 1102 96-97 0.10017382716861079 1102 98-99 -0.19384089027038698 1102 100-101 0.054211766375640735 1103 1 0.270312064693055 1103 2 0.29532557306215423 1103 3 0.34821568210147547 1103 4 0.37008799898762135 1103 5 0.35364707904388126 1103 6 0.3102490509574096 1103 7 0.38127290001231984 1103 8 0.3702941578021921 1103 9 0.5445534040014124 1103 10-11 0.4871321477407804 1103 12-13 0.5052678493702487 1103 14-15 0.5645640358285746 1103 16-17 0.6455473846287205 1103 18-19 0.7419581966367375 1103 20-21 0.6500262672626178 1103 22-23 0.644539688474218 1103 24-25 0.6488622529343218 1103 26-27 0.7127475379599346 1103 28-29 0.6646003761780435 1103 30-31 0.7840415717715103 1103 32-33 0.7065346615542083 1103 34-35 0.7025786466953647 1103 36-37 0.7477382086625326 1103 38-39 0.7526885690521965 1103 40-41 0.7994822142323059 1103 42-43 0.9412260730017366 1103 44-45 0.9556740288055181 1103 46-47 0.8296836039849111 1103 48-49 0.8819036127932307 1103 50-51 0.8990873437096099 1103 52-53 0.8719490715850142 1103 54-55 0.8795368119771823 1103 56-57 0.9319423291333422 1103 58-59 0.9618705448356337 1103 60-61 0.847061863872085 1103 62-63 0.8173848764281217 1103 64-65 0.6356347452188444 1103 66-67 0.6488366724996979 1103 68-69 0.6765934023347171 1103 70-71 0.6380740580206599 1103 72-73 0.6709711634428892 1103 74-75 0.48169551928195986 1103 76-77 0.5149054639308837 1103 78-79 0.5161772199802606 1103 80-81 0.5877085848179426 1103 82-83 0.6496153662206332 1103 84-85 0.5629121227742822 1103 86-87 0.55856358020689 1103 88-89 0.5796894671066397 1103 90-91 0.5028406107901624 1103 92-93 0.3126622745561072 1103 94-95 0.32005673538238355 1103 96-97 0.31172258949959186 1103 98-99 0.4848665879844276 1103 100-101 0.8433609919074101 1104 1 0.117892939751016 1104 2 0.174764169289773 1104 3 0.1286450178756553 1104 4 0.23880460930077163 1104 5 0.15009483861406636 1104 6 0.2805625507176188 1104 7 0.31114563608042545 1104 8 0.27266629249245966 1104 9 0.39161554285298195 1104 10-11 0.33845439986146175 1104 12-13 0.401342977287392 1104 14-15 0.4516699385694025 1104 16-17 0.5118851612712163 1104 18-19 0.5452248295511097 1104 20-21 0.6175943328683786 1104 22-23 0.37500207394287344 1104 24-25 0.34123836110080674 1104 26-27 0.37453215746271695 1104 28-29 0.36987028247997245 1104 30-31 0.4694301147203177 1104 32-33 0.458358446788381 1104 34-35 0.5467203987896809 1104 36-37 0.6649664279678049 1104 38-39 0.5041195283030504 1104 40-41 0.5315395900601345 1104 42-43 0.56211919108285 1104 44-45 0.6122265915933269 1104 46-47 0.7372586829987924 1104 48-49 0.7592591600289822 1104 50-51 0.799112157066844 1104 52-53 0.8685562084547129 1104 54-55 0.7892420693620252 1104 56-57 0.6607215175229939 1104 58-59 0.7426967760671843 1104 60-61 0.6708565239151767 1104 62-63 0.7240562191639555 1104 64-65 0.7564278307580778 1104 66-67 0.6170804099269347 1104 68-69 0.6384957165924448 1104 70-71 0.7222981192277693 1104 72-73 0.4947612322844783 1104 74-75 0.5259547017893134 1104 76-77 0.616760712808091 1104 78-79 0.45185657380621436 1104 80-81 0.4569453226106681 1104 82-83 0.47626185803487076 1104 84-85 0.5536491998289712 1104 86-87 0.42931388409573756 1104 88-89 0.5647928616601661 1104 90-91 0.5445255205264452 1104 92-93 0.6112844549844816 1104 94-95 0.6830249527204018 1104 96-97 0.5923156781274486 1104 98-99 0.7046980811502834 1104 100-101 1.0579872799307317 1105 1 0.1772987819000882 1105 2 0.17510831689338247 1105 3 0.2116355225673061 1105 4 0.3498558598322461 1105 5 0.3019963691104266 1105 6 0.3699985179570433 1105 7 0.37442638544559514 1105 8 0.3440753645425474 1105 9 0.4153171533528379 1105 10-11 0.38041612507448264 1105 12-13 0.4014036495359008 1105 14-15 0.4944575706837284 1105 16-17 0.47898270912484975 1105 18-19 0.5450866767444396 1105 20-21 0.48417534817686203 1105 22-23 0.48273126522170884 1105 24-25 0.49307397732049196 1105 26-27 0.5086074440799067 1105 28-29 0.5398821409622556 1105 30-31 0.47452069899610194 1105 32-33 0.49417560187002607 1105 34-35 0.5268322792276052 1105 36-37 0.6243280612187405 1105 38-39 0.6227951227862434 1105 40-41 0.7048052261508317 1105 42-43 0.6910363558098354 1105 44-45 0.7236846573295779 1105 46-47 0.6669902086373085 1105 48-49 0.6701316269626005 1105 50-51 0.652590706078854 1105 52-53 0.7007460356192965 1105 54-55 0.6808888399953616 1105 56-57 0.5944469613266321 1105 58-59 0.7018315478671582 1105 60-61 0.7739127683747 1105 62-63 0.6150513549676617 1105 64-65 0.5774461518832439 1105 66-67 0.6366840733673556 1105 68-69 0.6175364984893079 1105 70-71 0.5531845076354109 1105 72-73 0.7130761819941505 1105 74-75 0.6439035820718075 1105 76-77 0.6603373547093909 1105 78-79 0.6388637639749568 1105 80-81 0.7218334604352918 1105 82-83 0.6099618154357813 1105 84-85 0.6335452507487496 1105 86-87 0.7055306197069093 1105 88-89 0.5863586669702912 1105 90-91 0.6111083756177074 1105 92-93 0.6586399566181704 1105 94-95 0.8465912810158436 1105 96-97 0.815504462703359 1105 98-99 0.8452789952707143 1105 100-101 1.3526167240038376 1106 1 0.13443470914018363 1106 2 0.15720176643964834 1106 3 0.1867800663401482 1106 4 0.1755278841246266 1106 5 0.22088811025586352 1106 6 0.17979124889370723 1106 7 0.21337836989474823 1106 8 0.18683895512073434 1106 9 0.23124784779059127 1106 10-11 0.30825203896646514 1106 12-13 0.3610529128954951 1106 14-15 0.4998257338091108 1106 16-17 0.5339595214598987 1106 18-19 0.4355453089945698 1106 20-21 0.5421566690985742 1106 22-23 0.5066161735919366 1106 24-25 0.4002525679187272 1106 26-27 0.27287025851556024 1106 28-29 0.24801452691031045 1106 30-31 0.15490699971720545 1106 32-33 0.3229418451147197 1106 34-35 0.36699537004347604 1106 36-37 0.48847610553605847 1106 38-39 0.43193428249126953 1106 40-41 0.6714503535668186 1106 42-43 0.7360216679555691 1106 44-45 0.7298254140105556 1106 46-47 0.6570536519393428 1106 48-49 0.7057898213998612 1106 50-51 0.5925762788118476 1106 52-53 0.40524571039457413 1106 54-55 0.3648077903275677 1106 56-57 0.620074436042934 1106 58-59 0.7132003650566858 1106 60-61 0.7848082237264435 1106 62-63 0.7379680946130023 1106 64-65 0.7198324890142658 1106 66-67 0.7782183098989535 1106 68-69 0.8415702767751014 1106 70-71 0.8109522751676614 1106 72-73 0.7616202681981257 1106 74-75 0.6971494608334723 1106 76-77 0.791572890555976 1106 78-79 0.7864145264046236 1106 80-81 0.7731469680626084 1106 82-83 0.8062264401148873 1106 84-85 0.8933483427397313 1106 86-87 0.9036822509929685 1106 88-89 1.0446665139063995 1106 90-91 1.1440149756914622 1106 92-93 1.1955173083524038 1106 94-95 1.2613977008341948 1106 96-97 1.229839328035613 1106 98-99 1.2961038147179202 1106 100-101 1.6845975886842623 1107 1 0.06527603759105105 1107 2 0.08163673916428138 1107 3 0.14799918904373044 1107 4 0.20711355811373267 1107 5 0.1290461774380134 1107 6 0.03578940638756478 1107 7 0.09537656254342153 1107 8 0.06958771932249874 1107 9 0.10577692533338734 1107 10-11 0.11176091580159664 1107 12-13 0.11110943163982512 1107 14-15 0.2110372259483171 1107 16-17 0.1906742127686627 1107 18-19 0.23220931495708896 1107 20-21 0.11937364374489334 1107 22-23 0.1045502865844199 1107 24-25 -0.05707115577697408 1107 26-27 -0.008394874273498942 1107 28-29 0.013352347355002792 1107 30-31 -0.03803552496631113 1107 32-33 -0.007344825907416919 1107 34-35 0.07212664121336587 1107 36-37 0.07028101245713714 1107 38-39 0.09880693880325708 1107 40-41 0.09865518009181784 1107 42-43 0.07505889607461569 1107 44-45 0.30918668402558325 1107 46-47 0.33926990295517356 1107 48-49 0.16511296651972884 1107 50-51 0.12311979348100976 1107 52-53 0.12329875505546539 1107 54-55 0.21982414306235398 1107 56-57 0.30511706781719283 1107 58-59 0.375486271872461 1107 60-61 0.4825482953272484 1107 62-63 0.33188619804074193 1107 64-65 0.2734331215379484 1107 66-67 0.024996511487458406 1107 68-69 0.22785870290918808 1107 70-71 0.3511312295618545 1107 72-73 0.3582966276254709 1107 74-75 0.4397257594955697 1107 76-77 0.3922771530434481 1107 78-79 0.4250280898190475 1107 80-81 0.30324777857803653 1107 82-83 0.15534897449390428 1107 84-85 0.3774479878897381 1107 86-87 0.29590595844189593 1107 88-89 0.45609506233650166 1107 90-91 0.4879328409207453 1107 92-93 0.5896833845880671 1107 94-95 0.5693275574666536 1107 96-97 0.5807637926763682 1107 98-99 0.8318814365806624 1107 100-101 1.1503089371651534 1108 1 0.3199871571870183 1108 2 0.20935701000747997 1108 3 0.14967919043215971 1108 4 0.12843613771917006 1108 5 0.22523964536649999 1108 6 0.20097326191141462 1108 7 0.25411586728664304 1108 8 0.2684080489260765 1108 9 0.299276837316782 1108 10-11 0.3424039576553497 1108 12-13 0.46638125417852194 1108 14-15 0.46128255672957863 1108 16-17 0.5766562672992848 1108 18-19 0.604341771264906 1108 20-21 0.5020597864607126 1108 22-23 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2316 54-55 -2.4980816839342808 2316 56-57 -2.2309275033046845 2316 58-59 -1.9735425881010755 2316 60-61 -1.78495863076909 2316 62-63 -2.117038838946687 2316 64-65 -2.3007248130183626 2316 66-67 -2.025075507396277 2316 68-69 -2.293592518673478 2316 70-71 -2.0904555486943117 2316 72-73 -2.093438358530161 2316 74-75 -2.118476530243324 2316 76-77 -2.1501612553587783 2316 78-79 -1.8818028680516612 2316 80-81 -1.8360670210091747 2316 82-83 -1.9244005359263419 2316 84-85 -1.8650242197061608 2316 86-87 -1.93245594642433 2316 88-89 -2.0045821577111127 2316 90-91 -2.2789534944251493 2316 92-93 -2.021781818281422 2316 94-95 -2.017741590832788 2316 96-97 -2.240625229001278 2316 98-99 -2.5685450435228 2316 100-101 -2.546470750771274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28125.0 3 7405.0 4 7125.0 5 7948.0 6 9060.0 7 10767.0 8 13203.0 9 15205.0 10 18376.0 11 22145.0 12 26872.0 13 31184.0 14 33313.0 15 36093.0 16 41719.0 17 49324.0 18 44912.0 19 36401.0 20 37346.0 21 41434.0 22 45693.0 23 51258.0 24 58483.0 25 66420.0 26 75885.0 27 88670.0 28 102583.0 29 119921.0 30 141924.0 31 170546.0 32 207099.0 33 257576.0 34 335632.0 35 458560.0 36 659874.0 37 952144.0 38 837787.0 39 125013.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.96246414480365 19.01243892921608 13.431369864368284 23.59372706161199 2 15.391750554349207 20.542615411297383 40.02894222401881 24.03669181033459 3 19.24721054850741 24.981069865639274 29.55495135359249 26.216768232260822 4 12.527895022775711 16.334649094480262 37.398326108677566 33.73912977406646 5 13.567808532830577 37.788624451321986 34.16551993413678 14.478047081710658 6 31.885591851594558 35.75393347840264 17.96524721192558 14.395227458077226 7 28.767622502053108 32.60170483924932 20.971575873711103 17.659096784986463 8 26.48209358325675 34.58605563010199 20.37809047144483 18.55376031519643 9 27.08866466840916 16.278321995482077 20.48932492145678 36.14368841465198 10-11 24.95353884403485 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28.731787217949663 21.992917491979078 36-37 22.375126761089 26.58318554853136 28.679575004293596 22.362112686086043 38-39 22.17502148305209 26.871043434790703 28.89814120675929 22.055793875397917 40-41 22.211871124423865 26.701166872456977 28.965053769835247 22.12190823328391 42-43 22.167717851841665 26.75022212661907 28.693333917104773 22.38872610443449 44-45 22.11120702940535 27.078000991673957 28.463660213551627 22.347131765369067 46-47 22.16626644157853 27.083466207554896 28.132422116032846 22.617845234833727 48-49 22.34936583809039 27.129601877407172 28.000401028430367 22.520631256072075 50-51 22.293115273256124 27.28626440101708 27.871082883367993 22.5495374423588 52-53 22.502315128415727 27.239790942975816 27.888912298546696 22.368981630061757 54-55 22.06846857893351 27.082414267194014 28.130906497344615 22.718210656527855 56-57 22.25542901757199 27.504706349400482 27.814808113528404 22.42505651949912 58-59 22.32095917394253 26.98019743097847 27.972138563459975 22.726704831619024 60-61 22.331385310601615 27.04026582676914 28.2419634668926 22.386385395736646 62-63 22.30638560831798 26.967450957696563 28.172053368748017 22.554110065237438 64-65 22.053056962612295 26.877036116737962 28.135274611410804 22.93463230923894 66-67 22.020501464968497 27.240548598778986 27.874366845937914 22.8645830903146 68-69 22.135453270086433 27.012261990875597 28.042709560589458 22.809575178448515 70-71 21.980066246500478 26.98691173091385 28.074428132793543 22.958593889792127 72-73 22.067141826591637 26.88340172660314 27.97800192115641 23.07145452564881 74-75 21.89250263293777 27.088937738508072 27.903913029692756 23.1146465988614 76-77 22.06160296652643 26.888234606993418 28.035858633603123 23.014303792877037 78-79 22.01538513373192 26.86854356516553 27.950884220701212 23.165187080401335 80-81 21.7648399837992 27.108562007794852 27.99281910551984 23.133778902886114 82-83 22.049980408724647 26.94842753836482 27.846532344262688 23.155059708647844 84-85 21.770693532962156 26.77004524320005 27.918603364899937 23.54065785893786 86-87 21.98026056390719 26.558205939193886 28.140681130677763 23.32085236622116 88-89 22.087631959971922 26.40959682230782 28.025194048072265 23.47757716964799 90-91 21.763526504544636 26.645917063033803 28.23693054916527 23.353625883256292 92-93 21.946121117935242 26.458394888418148 28.155282304671893 23.44020168897472 94-95 21.803151116424317 26.213417127451148 28.117011103583994 23.866420652540533 96-97 21.962629860591658 26.051204794896567 28.10644342794347 23.879721916568304 98-99 21.72630199691186 26.053174485655095 27.998588768328993 24.221934749104054 100-101 22.029882176318072 25.601168288469765 28.54964964197842 23.81929989323374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4679.0 1 3128.5 2 1519.5 3 1435.5 4 1401.0 5 1476.0 6 2008.0 7 3205.5 8 4582.5 9 5200.5 10 4828.5 11 4547.5 12 4844.0 13 5191.0 14 5510.0 15 5881.0 16 6263.5 17 6621.0 18 6976.5 19 7434.5 20 8250.0 21 9532.0 22 11243.0 23 13575.0 24 16182.5 25 20176.5 26 26759.0 27 34514.0 28 40972.5 29 47981.5 30 57133.0 31 67823.0 32 80333.0 33 92276.0 34 105671.5 35 117379.5 36 129252.0 37 143614.5 38 151630.0 39 153358.0 40 154370.0 41 161534.0 42 171847.0 43 181312.5 44 190048.0 45 196559.5 46 197946.5 47 198792.5 48 216289.5 49 221359.5 50 206866.0 51 196033.5 52 185617.0 53 181134.0 54 174425.5 55 159190.0 56 142833.5 57 127094.5 58 113969.5 59 100526.5 60 85450.0 61 69897.0 62 55669.5 63 43209.5 64 31509.5 65 23635.0 66 17413.0 67 13319.5 68 10122.0 69 7246.0 70 5769.0 71 4598.5 72 3665.0 73 3013.5 74 2314.5 75 1862.0 76 1389.0 77 867.5 78 575.0 79 441.0 80 307.5 81 211.5 82 187.0 83 152.5 84 96.0 85 73.0 86 66.0 87 48.5 88 31.5 89 27.0 90 23.0 91 20.0 92 17.0 93 12.0 94 11.5 95 11.0 96 6.5 97 4.5 98 3.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2735594416694465 2 0.16680962557535192 3 0.19540772873219947 4 0.16493216389595675 5 0.23663602864134164 6 0.19542669299158727 7 0.24192705701054623 8 0.24879211890894065 9 0.14835740119099342 10-11 0.20643544556622254 12-13 0.21226695532798023 14-15 0.17330488441568367 16-17 0.2876214400055224 18-19 0.23281473037469394 20-21 0.1985463136608853 22-23 0.24467687462178156 24-25 0.23331728324847145 26-27 0.3447038607628904 28-29 0.315764400937062 30-31 0.29080743558267785 32-33 0.19188037648606307 34-35 0.36043471392509535 36-37 0.39959590956096414 38-39 0.38116264943599343 40-41 0.34386943334982595 42-43 0.3769999945003648 44-45 0.38578044659693 46-47 0.3909671715395015 48-49 0.40922975332998174 50-51 0.40187162068750376 52-53 0.4022034952267908 54-55 0.3966564493558504 56-57 0.3755207822681141 58-59 0.3495207826473992 60-61 0.3171108633535979 62-63 0.3843107164943732 64-65 0.4165689217130719 66-67 0.42255214554993226 68-69 0.4275112993798498 70-71 0.42736906743444103 72-73 0.41859809746756965 74-75 0.41367687215642784 76-77 0.39841064334922477 78-79 0.3734821243839223 80-81 0.38401677047386185 82-83 0.3932997754442046 84-85 0.3943807382293109 86-87 0.3896396733823534 88-89 0.3975572516767724 90-91 0.4182188122798131 92-93 0.41762143810909647 94-95 0.42536833806902496 96-97 0.42659153279954004 98-99 0.42374689389136555 100-101 0.3972917520453428 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5273077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.875006644253205 #Duplication Level Percentage of deduplicated Percentage of total 1 67.08089008310253 26.077700476830294 2 14.445762416925852 11.231582198785913 3 6.409985757800059 7.47564716772137 4 3.451374596173582 5.3668884143221876 5 2.174903675415365 4.22746974161915 6 1.3855561328418353 3.231810232212727 7 0.9917364982658288 2.698763407160275 8 0.7026324726847252 2.1851873635269397 9 0.5328434439452743 1.8642863181347362 >10 2.5190474827422054 16.86235516257702 >50 0.17149166967502738 4.6281071768727795 >100 0.12162007145397247 9.295028262143875 >500 0.008969732062972403 2.3457869429609857 >1k 0.003038922284008134 1.8603515256039413 >5k 4.9014875548410833E-5 0.1439189113605628 >10k+ 9.802975109682167E-5 0.5051166981672395 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15182 0.2879153860260337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11327 0.21480816608594944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7553 0.14323705115627935 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.792851877565983E-5 0.0 5 0.0 0.0 0.0 3.792851877565983E-5 0.0 6 0.0 0.0 0.0 3.792851877565983E-5 0.0 7 0.0 0.0 0.0 3.792851877565983E-5 0.0 8 0.0 0.0 0.0 3.792851877565983E-5 0.0 9 0.0 0.0 0.0 3.792851877565983E-5 0.0 10-11 0.0 0.0 0.0 5.6892778163489744E-5 0.0 12-13 0.0 0.0 0.0 7.585703755131965E-5 0.0 14-15 0.0 0.0 0.0 9.482129693914957E-5 0.0 16-17 0.0 0.0 0.0 1.0430342663306452E-4 0.0 18-19 1.8964259387829914E-5 0.0 0.0 1.611962047965543E-4 0.0 20-21 1.8964259387829914E-5 0.0 0.0 2.844638908174487E-4 1.8964259387829914E-5 22-23 3.792851877565983E-5 0.0 0.0 5.404813925531525E-4 1.8964259387829914E-5 24-25 3.792851877565983E-5 0.0 0.0 0.0010051057475549854 1.8964259387829914E-5 26-27 3.792851877565983E-5 0.0 0.0 0.0017731582527620968 1.8964259387829914E-5 28-29 6.63749078574047E-5 0.0 0.0 0.004693654198487903 1.8964259387829914E-5 30-31 7.585703755131965E-5 0.0 0.0 0.015076586213324781 1.8964259387829914E-5 32-33 7.585703755131965E-5 0.0 0.0 0.035510575703711515 1.8964259387829914E-5 34-35 8.533916724523462E-5 0.0 0.0 0.07036688445854289 1.8964259387829914E-5 36-37 9.482129693914957E-5 0.0 0.0 0.1303508369022489 1.8964259387829914E-5 38-39 9.482129693914957E-5 0.0 0.0 0.21638219961513933 1.8964259387829914E-5 40-41 1.0430342663306452E-4 0.0 0.0 0.3118103528546995 1.8964259387829914E-5 42-43 1.327498157148094E-4 0.0 0.0 0.4103581267635576 1.8964259387829914E-5 44-45 1.517140751026393E-4 0.0 0.0 0.5302312103540305 1.8964259387829914E-5 46-47 1.517140751026393E-4 0.0 0.0 0.6776119521865507 1.8964259387829914E-5 48-49 1.517140751026393E-4 0.0 0.0 0.8422880985807717 1.8964259387829914E-5 50-51 1.517140751026393E-4 0.0 0.0 1.0191108531128978 1.8964259387829914E-5 52-53 1.517140751026393E-4 0.0 0.0 1.192576933733378 1.8964259387829914E-5 54-55 1.7067833449046924E-4 0.0 0.0 1.385576201523323 1.8964259387829914E-5 56-57 2.0860685326612905E-4 0.0 0.0 1.612815439637995 1.8964259387829914E-5 58-59 2.0860685326612905E-4 0.0 0.0 1.8670787473803245 1.8964259387829914E-5 60-61 2.0860685326612905E-4 0.0 0.0 2.148204928545515 1.8964259387829914E-5 62-63 2.2757111265395898E-4 0.0 0.0 2.427956200905088 1.8964259387829914E-5 64-65 2.465353720417889E-4 0.0 0.0 2.7162698363782662 3.792851877565983E-5 66-67 2.654996314296188E-4 0.0 0.0 3.0455367899994634 5.6892778163489744E-5 68-69 2.844638908174487E-4 0.0 0.0 3.421237732731762 5.6892778163489744E-5 70-71 3.034281502052786E-4 0.0 0.0 3.8299270046691904 5.6892778163489744E-5 72-73 3.034281502052786E-4 0.0 0.0 4.244865000074909 5.6892778163489744E-5 74-75 3.034281502052786E-4 0.0 0.0 4.66581466570657 5.6892778163489744E-5 76-77 3.034281502052786E-4 0.0 0.0 5.116092558481509 5.6892778163489744E-5 78-79 3.034281502052786E-4 0.0 0.0 5.615999918074399 5.6892778163489744E-5 80-81 3.8876731745051324E-4 0.0 0.0 6.168163294410455 5.6892778163489744E-5 82-83 3.982494471444282E-4 0.0 0.0 6.730235496276652 5.6892778163489744E-5 84-85 4.172137065322581E-4 0.0 0.0 7.290657807576108 5.6892778163489744E-5 86-87 4.45660095614003E-4 0.0 0.0 7.87715597553383 5.6892778163489744E-5 88-89 4.5514222530791795E-4 0.0 0.0 8.49753379288791 5.6892778163489744E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 22925 0.0 32.134052 1 GGTATCA 15115 0.0 31.915457 1 GTACATG 25620 0.0 28.141653 1 TACATGG 26125 0.0 27.13118 2 ATCAACG 27990 0.0 26.153957 3 TCAACGC 28020 0.0 26.109022 4 ACATGGG 26315 0.0 25.889605 3 CAACGCA 28460 0.0 25.722038 5 TATCAAC 29035 0.0 25.245329 2 AACGCAG 29120 0.0 25.15343 6 CATGGGA 15855 0.0 22.262865 4 ACGCAGA 32825 0.0 22.25737 7 CGCAGAG 33115 0.0 21.918982 8 CATGGGG 10625 0.0 21.835056 4 GCAGAGT 35070 0.0 20.127983 9 GAGTACT 15200 0.0 20.017498 12-13 AGTACTT 15760 0.0 19.306217 12-13 GTACTTT 16510 0.0 18.587852 14-15 AGTACAT 18985 0.0 18.092657 2 AGAGTAC 31240 0.0 17.798664 10-11 >>END_MODULE