FastQCFastQC Report
Thu 26 May 2016
SRR938756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273077
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148970.28251057210050223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122740.23276731972622436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80110.15192268195590544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155700.033.0621381
GTATCAA231800.030.7050931
GTACATG253000.025.6123161
ATCAACG284700.024.7223683
TCAACGC285850.024.6063044
TACATGG261200.024.3846042
CAACGCA289400.024.3372635
TATCAAC295600.023.891032
AACGCAG296200.023.7868946
ACATGGG266600.022.8937633
ACGCAGA334050.021.0052727
CGCAGAG336150.020.7611378
GAGTACT158850.020.50529912-13
CATGGGG100850.019.4361614
CGTATAG2702.2611857E-819.3830071
CATGGGA160200.019.1976854
GCAGAGT362200.018.9928959
GTACTTT172400.018.9211814-15
AGTACTT167950.018.82924712-13
AGTACAT196600.018.6126082
Produced by FastQC (version 0.11.5)