##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938756_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5273077 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06546614054754 33.0 31.0 34.0 30.0 34.0 2 32.267830528550974 34.0 31.0 34.0 30.0 34.0 3 32.353620096956675 34.0 31.0 34.0 30.0 34.0 4 35.97355547813923 37.0 35.0 37.0 35.0 37.0 5 35.87903097185951 37.0 35.0 37.0 35.0 37.0 6 35.902943196164216 37.0 35.0 37.0 35.0 37.0 7 35.81773734766247 37.0 35.0 37.0 33.0 37.0 8 35.79931755216167 37.0 35.0 37.0 33.0 37.0 9 37.4444272291112 39.0 37.0 39.0 34.0 39.0 10-11 37.37164477211313 39.0 37.0 39.0 34.0 39.0 12-13 37.296248281601045 39.0 37.0 39.0 34.0 39.0 14-15 38.52532136359852 40.0 38.0 41.0 34.0 41.0 16-17 38.45449288906648 40.0 38.0 41.0 33.5 41.0 18-19 38.587293339353856 40.0 38.0 41.0 34.0 41.0 20-21 38.5875396850833 40.0 38.0 41.0 34.0 41.0 22-23 38.516144748123345 40.0 38.0 41.0 34.0 41.0 24-25 38.44307090527978 40.0 38.0 41.0 34.0 41.0 26-27 38.2805033000656 40.0 38.0 41.0 33.5 41.0 28-29 38.11893605953412 40.0 38.0 41.0 33.0 41.0 30-31 37.91092278758683 40.0 37.0 41.0 33.0 41.0 32-33 37.793441950496835 40.0 37.0 41.0 33.0 41.0 34-35 37.77826077639298 40.0 37.0 41.0 33.0 41.0 36-37 37.6780222439384 40.0 37.0 41.0 33.0 41.0 38-39 37.57108562988934 40.0 37.0 41.0 32.0 41.0 40-41 37.40719014723282 40.0 37.0 41.0 32.0 41.0 42-43 37.22840136792996 40.0 36.0 41.0 31.0 41.0 44-45 36.998240211550105 40.0 36.0 41.0 31.0 41.0 46-47 36.71919725427867 40.0 36.0 41.0 30.0 41.0 48-49 36.44830105837635 39.0 35.5 41.0 30.0 41.0 50-51 36.237261375094654 39.0 35.0 41.0 30.0 41.0 52-53 36.029416695413325 39.0 35.0 40.5 29.0 41.0 54-55 35.76394607930057 39.0 35.0 40.0 28.0 41.0 56-57 35.50999217345015 38.0 35.0 40.0 28.0 41.0 58-59 35.2185269625306 38.0 34.0 40.0 27.5 41.0 60-61 34.88000497622166 38.0 34.0 40.0 26.5 41.0 62-63 34.52804311410586 37.0 34.0 40.0 26.0 41.0 64-65 34.13609852463751 37.0 33.5 39.5 26.0 41.0 66-67 33.74047629116738 36.0 33.0 39.0 26.0 40.5 68-69 33.40957717856197 36.0 33.0 39.0 25.0 40.0 70-71 32.98683965737652 35.0 32.0 38.5 24.0 40.0 72-73 32.51509716243476 35.0 32.0 37.5 22.5 40.0 74-75 32.01778344977705 35.0 31.0 37.0 22.0 39.0 76-77 30.05057056819007 33.0 29.0 35.0 19.5 37.5 78-79 31.01386941628199 34.0 30.5 36.0 20.0 38.0 80-81 31.070211093067673 34.0 31.0 36.0 20.0 37.5 82-83 30.753141287335648 34.0 31.0 35.0 19.0 37.0 84-85 30.36211693855409 34.0 30.5 35.0 17.5 36.5 86-87 29.784875889352648 34.0 30.0 35.0 9.5 36.0 88-89 29.19847387019002 34.0 29.0 35.0 4.5 36.0 90-91 28.68933129556045 34.0 29.0 35.0 2.0 35.0 92-93 28.39985875419608 34.0 29.0 35.0 2.0 35.0 94-95 28.06746924044538 33.5 28.5 35.0 2.0 35.0 96-97 27.471004595608978 33.0 27.0 35.0 2.0 35.0 98-99 26.649882412109665 33.0 25.5 35.0 2.0 35.0 100-101 23.0017966739344 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.054532031278753834 1101 2 0.12620579734394255 1101 3 0.05750327040529157 1101 4 0.08222703577858681 1101 5 -0.08176658896944389 1101 6 0.053427365649973524 1101 7 0.20089872954171284 1101 8 0.19443258693927135 1101 9 0.20476734514814154 1101 10-11 0.2387977613193044 1101 12-13 0.15194087502060682 1101 14-15 0.2817770124987291 1101 16-17 0.34288585689537854 1101 18-19 0.24558546891874045 1101 20-21 0.23463925423079246 1101 22-23 0.14497695146734912 1101 24-25 0.16265748008056846 1101 26-27 0.07528077092063512 1101 28-29 0.0737289925314002 1101 30-31 0.1882285658221292 1101 32-33 0.2676046441818656 1101 34-35 0.30993830268270983 1101 36-37 0.3842798215301215 1101 38-39 0.41896931325529607 1101 40-41 0.35955907015707567 1101 42-43 0.4177676911257677 1101 44-45 0.3281037845684338 1101 46-47 0.359934184969525 1101 48-49 0.4291211960568617 1101 50-51 0.3403341287129038 1101 52-53 0.1874882283363135 1101 54-55 0.2287916519837765 1101 56-57 0.24619452929056962 1101 58-59 0.21884079673719015 1101 60-61 0.3203046496305788 1101 62-63 0.1472521329207055 1101 64-65 0.14747718634393436 1101 66-67 0.19009063180494223 1101 68-69 0.15088452955272658 1101 70-71 0.16753221646232674 1101 72-73 0.11575760675795976 1101 74-75 0.05910766167296089 1101 76-77 -0.0038876562601863895 1101 78-79 -0.1319189414425459 1101 80-81 0.08956049727853 1101 82-83 0.20093059453994755 1101 84-85 0.25168220152084686 1101 86-87 0.2892309026247979 1101 88-89 0.27586935593215856 1101 90-91 0.04590326035462411 1101 92-93 0.10569214455261644 1101 94-95 0.22392586772528844 1101 96-97 0.2656580627925287 1101 98-99 -0.00349542754477028 1101 100-101 0.6892794706132435 1102 1 0.2762789886505672 1102 2 0.19321518756268574 1102 3 0.1974111440185098 1102 4 0.13136548545078597 1102 5 0.0025379160036891335 1102 6 -0.00836769977662044 1102 7 0.04963068229465506 1102 8 0.10816422578805174 1102 9 0.18134130545271887 1102 10-11 0.2346410065502056 1102 12-13 0.2434635971974899 1102 14-15 0.3269897588013819 1102 16-17 0.3505504458154576 1102 18-19 0.3317486273327077 1102 20-21 0.32449175515007767 1102 22-23 0.22673375646111538 1102 24-25 0.23147651881635056 1102 26-27 0.23437929198360052 1102 28-29 0.29457343059811336 1102 30-31 0.2959132984206292 1102 32-33 0.34697929328186206 1102 34-35 0.3981118827148151 1102 36-37 0.3382839291902755 1102 38-39 0.3668261012085523 1102 40-41 0.43906299395908377 1102 42-43 0.4327278432347583 1102 44-45 0.34034694369990603 1102 46-47 0.35979644382386766 1102 48-49 0.3042070712665108 1102 50-51 0.3689910135280101 1102 52-53 0.24005790160692442 1102 54-55 0.17801327728983551 1102 56-57 0.26577344928088564 1102 58-59 0.19150394169813723 1102 60-61 0.1977470840918727 1102 62-63 0.14129355033252722 1102 64-65 0.2062903416188675 1102 66-67 0.32668638602694955 1102 68-69 0.2818686460022022 1102 70-71 0.28048910396804416 1102 72-73 0.31552759572647204 1102 74-75 0.28511623574088674 1102 76-77 0.2443070001941301 1102 78-79 0.09675849961073624 1102 80-81 0.39767976292216645 1102 82-83 0.3286177845595901 1102 84-85 0.45161533096595363 1102 86-87 0.5361071974794633 1102 88-89 0.34743814130242967 1102 90-91 0.36968724048000823 1102 92-93 0.5040495647985388 1102 94-95 0.49305468502409866 1102 96-97 0.603309952047141 1102 98-99 0.7090950710247235 1102 100-101 1.4218994941112122 1103 1 0.16989597732304418 1103 2 0.17891812958422548 1103 3 0.14379533946834755 1103 4 0.03452834580271258 1103 5 0.06118596176654023 1103 6 0.10091056026011813 1103 7 0.1456420359380104 1103 8 0.08088493835106902 1103 9 0.13851316861693164 1103 10-11 0.16162313152734953 1103 12-13 0.15299600819241022 1103 14-15 0.2318431627176878 1103 16-17 0.12095855008787737 1103 18-19 0.021068174928338124 1103 20-21 0.12704407855478195 1103 22-23 0.20148936580523724 1103 24-25 0.23810161350259307 1103 26-27 0.24532004233405047 1103 28-29 0.13794018636417604 1103 30-31 0.25050499389223546 1103 32-33 0.3437598040216727 1103 34-35 0.25533065801889876 1103 36-37 0.29408753902198015 1103 38-39 0.22891200231692466 1103 40-41 0.32715145486127994 1103 42-43 0.3275418750841652 1103 44-45 0.31003036511685167 1103 46-47 0.31874071171412766 1103 48-49 0.3682528515247512 1103 50-51 0.4047936656137239 1103 52-53 0.3660885494785546 1103 54-55 0.2733589452305978 1103 56-57 0.21391011092664058 1103 58-59 0.24365716364270185 1103 60-61 0.2996274180840217 1103 62-63 0.23653287364144404 1103 64-65 0.26568473271008486 1103 66-67 0.2907262456736319 1103 68-69 0.28354116652843686 1103 70-71 0.3383586415508617 1103 72-73 0.31713932550278656 1103 74-75 0.3755574083702484 1103 76-77 0.3927116391674268 1103 78-79 0.30076290524228355 1103 80-81 0.5428041392989265 1103 82-83 0.5559595344485793 1103 84-85 0.5243947272377092 1103 86-87 0.5807153487685035 1103 88-89 0.45195516741407005 1103 90-91 0.4103263182576917 1103 92-93 0.41232154724566783 1103 94-95 0.4978928868995389 1103 96-97 0.5212210558058565 1103 98-99 0.5744375871105021 1103 100-101 1.3521555605613713 1104 1 0.08165204094374445 1104 2 0.05194532160120957 1104 3 0.06475745774298503 1104 4 0.08476868128876447 1104 5 -0.09672159675632486 1104 6 0.004951944515724449 1104 7 0.0624272738617222 1104 8 -0.010829042038380976 1104 9 0.03929382591282149 1104 10-11 0.046637394164392276 1104 12-13 -0.043765184356878706 1104 14-15 -0.02373368627393546 1104 16-17 0.14918821339558974 1104 18-19 0.22081535276546305 1104 20-21 0.13747587966504682 1104 22-23 0.0937286144286773 1104 24-25 0.1062710708545751 1104 26-27 0.07101984231070446 1104 28-29 0.009730258266564817 1104 30-31 -0.0745950282151 1104 32-33 -0.07509884832711577 1104 34-35 -0.018728420449050986 1104 36-37 0.07076301105661997 1104 38-39 0.10758463309347377 1104 40-41 0.07700777112327728 1104 42-43 0.04750056540080294 1104 44-45 0.05354727555544514 1104 46-47 0.0856094121161064 1104 48-49 0.2419742192965444 1104 50-51 0.06939919584115017 1104 52-53 0.05832817374574262 1104 54-55 -0.01722143272545651 1104 56-57 -0.07482665615238915 1104 58-59 0.1629845359269595 1104 60-61 0.2708261849707654 1104 62-63 0.21248833397826417 1104 64-65 0.10335466455578768 1104 66-67 0.10611497227049682 1104 68-69 0.16537459606831106 1104 70-71 0.04961867454351676 1104 72-73 0.18277718738860216 1104 74-75 0.21650674501815104 1104 76-77 0.16809367930538244 1104 78-79 0.024775904742401877 1104 80-81 0.18271359663143372 1104 82-83 0.29559402750550134 1104 84-85 0.3091228884573347 1104 86-87 0.30870172013465336 1104 88-89 0.32226269861232737 1104 90-91 0.36624052317080213 1104 92-93 0.3809601280023287 1104 94-95 0.37765081510455545 1104 96-97 0.4979144822209314 1104 98-99 0.7116618871992237 1104 100-101 1.486826943922381 1105 1 0.06874386788457798 1105 2 0.06301039755921778 1105 3 0.039963679130806895 1105 4 0.08034109520688304 1105 5 -0.020514479421336773 1105 6 -0.026265855058070997 1105 7 0.043407775275582594 1105 8 0.10431092281906018 1105 9 0.12136822699926597 1105 10-11 0.11738536191462146 1105 12-13 0.16943554122099158 1105 14-15 0.06632523682814906 1105 16-17 0.15571315185418655 1105 18-19 0.1322165881525521 1105 20-21 0.04403479999392346 1105 22-23 -0.011921588481776269 1105 24-25 0.06219234411413055 1105 26-27 0.14957596319363375 1105 28-29 0.1789124896985257 1105 30-31 0.14797211946007138 1105 32-33 0.11151924807397506 1105 34-35 0.18571896358950823 1105 36-37 0.1441523522479926 1105 38-39 0.2044600866980062 1105 40-41 0.17889179662753207 1105 42-43 0.2445253390229709 1105 44-45 0.20748403193993425 1105 46-47 0.17942362273362988 1105 48-49 0.2055662707314596 1105 50-51 0.2113911286641894 1105 52-53 0.29626836376014154 1105 54-55 0.38491088228810355 1105 56-57 0.4706576781648266 1105 58-59 0.4863975507938889 1105 60-61 0.49330574004635963 1105 62-63 0.4068369676757513 1105 64-65 0.4254390789306015 1105 66-67 0.3507197077902262 1105 68-69 0.34930464806787853 1105 70-71 0.30819950262704054 1105 72-73 0.37852980532510117 1105 74-75 0.3982657180514053 1105 76-77 0.4114781721279961 1105 78-79 0.22062534801195355 1105 80-81 0.23175067325987087 1105 82-83 0.3925100983733927 1105 84-85 0.28439590342978605 1105 86-87 0.4305854020324169 1105 88-89 0.33553389057648175 1105 90-91 0.3884615481814322 1105 92-93 0.5363675607518417 1105 94-95 0.7941537613949379 1105 96-97 0.7728488824659472 1105 98-99 0.969351401397816 1105 100-101 1.6933723905479177 1106 1 0.1839960562095726 1106 2 0.14453273711617243 1106 3 0.09805048366927593 1106 4 0.10475996098788443 1106 5 -0.02591514201618139 1106 6 0.040391936964120134 1106 7 0.01793042576274928 1106 8 0.07251648482229456 1106 9 0.13577032252825205 1106 10-11 0.10743876619954307 1106 12-13 0.12628386951597292 1106 14-15 0.1565142384950562 1106 16-17 0.18223817384965457 1106 18-19 0.060889190863200326 1106 20-21 0.049127752748503895 1106 22-23 0.16084453961817502 1106 24-25 0.13502181496164667 1106 26-27 0.15039323119687253 1106 28-29 0.2334804955120191 1106 30-31 0.2201922170399797 1106 32-33 0.18632813049116237 1106 34-35 0.20202801231590684 1106 36-37 0.15061974667055011 1106 38-39 0.28497885315353244 1106 40-41 0.3886975766433878 1106 42-43 0.35305677507955835 1106 44-45 0.30850381862905607 1106 46-47 0.3378962771411054 1106 48-49 0.24076853605949822 1106 50-51 0.24664766383430248 1106 52-53 0.23626967688328904 1106 54-55 0.27916546242946083 1106 56-57 0.3279816389885397 1106 58-59 0.2860002028820432 1106 60-61 0.4139187043574779 1106 62-63 0.4350013726663349 1106 64-65 0.4964028041992137 1106 66-67 0.4234697866071926 1106 68-69 0.40265527107515453 1106 70-71 0.42304132353584123 1106 72-73 0.5095702950635186 1106 74-75 0.7140092858674763 1106 76-77 0.6120491079543235 1106 78-79 0.49316080545890983 1106 80-81 0.42254354070087885 1106 82-83 0.5751977985827779 1106 84-85 0.5788773587856006 1106 86-87 0.6862892889492507 1106 88-89 0.6968551547000281 1106 90-91 0.7052367687719929 1106 92-93 0.8373189736835265 1106 94-95 0.9752460301161925 1106 96-97 1.0107300471864704 1106 98-99 1.151247990912033 1106 100-101 1.7208339000362187 1107 1 0.04669708544046358 1107 2 0.03073682456884086 1107 3 -0.042002784076800026 1107 4 -0.03777737169207285 1107 5 -0.1314998773390954 1107 6 -0.13252318996855195 1107 7 -0.0011544289057994206 1107 8 0.01988625638669106 1107 9 -0.04962045682588467 1107 10-11 -0.06559955040431475 1107 12-13 0.06670976178686061 1107 14-15 0.03564100623562183 1107 16-17 0.0013684833548737174 1107 18-19 0.04973521133084802 1107 20-21 -0.02532841963883925 1107 22-23 9.040169516296714E-4 1107 24-25 -0.055555497604501625 1107 26-27 -0.005645847149224892 1107 28-29 0.020620111513103723 1107 30-31 -0.08270616536250941 1107 32-33 -0.0599867011739903 1107 34-35 0.034036973177606455 1107 36-37 0.013151737134073471 1107 38-39 0.07164841324698301 1107 40-41 -0.11339365796550283 1107 42-43 0.056627879208264176 1107 44-45 0.03901592395680353 1107 46-47 -0.06642096312113921 1107 48-49 0.057399190952480694 1107 50-51 -0.07813961262260705 1107 52-53 -0.08459639299493205 1107 54-55 0.02673587923241172 1107 56-57 -0.05946175396942266 1107 58-59 0.10510141932049066 1107 60-61 0.13718310415239188 1107 62-63 0.08145835131438872 1107 64-65 -0.0298978209813896 1107 66-67 0.0916456513209809 1107 68-69 0.09547797372084688 1107 70-71 -0.009602099451555546 1107 72-73 0.047608091737643576 1107 74-75 0.10391895279867924 1107 76-77 0.04314721434229796 1107 78-79 0.16134347114934755 1107 80-81 0.16817894822805002 1107 82-83 0.18101442421085778 1107 84-85 0.13950512507053148 1107 86-87 0.2630263840549958 1107 88-89 0.1477161107037901 1107 90-91 0.4327568386347771 1107 92-93 0.5045283933318778 1107 94-95 0.4743380504514434 1107 96-97 0.5348552469240886 1107 98-99 0.806631859225412 1107 100-101 1.3953362336418635 1108 1 0.17722124289844743 1108 2 0.0714200813318655 1108 3 0.06126275581207352 1108 4 0.07502916781746194 1108 5 0.053026804087913604 1108 6 0.07118912714906855 1108 7 0.0392184143775367 1108 8 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sequence quality scores pass #Quality Count 2 4.0 3 16.0 4 62.0 5 112.0 6 280.0 7 759.0 8 1739.0 9 3160.0 10 5112.0 11 7980.0 12 11430.0 13 15746.0 14 19131.0 15 24971.0 16 31632.0 17 41847.0 18 39376.0 19 28225.0 20 27049.0 21 28666.0 22 31239.0 23 35574.0 24 41717.0 25 50066.0 26 60831.0 27 74947.0 28 91413.0 29 112506.0 30 136925.0 31 168474.0 32 205647.0 33 259471.0 34 342727.0 35 473209.0 36 711761.0 37 1120754.0 38 974595.0 39 93918.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61888858010411 19.88980311992588 14.003588070634581 23.487720229335423 2 15.862104801428083 21.22157518276331 39.055925032006925 23.860394983801676 3 19.229322841293612 25.158820931308227 29.837170972470155 25.774685254928002 4 12.985511116185103 17.0161368779557 36.88284847727428 33.115503528584924 5 13.435750093948004 37.98663500731191 34.30719299300602 14.270421905734063 6 31.885837373331068 36.35707795654724 17.52297929469159 14.2341053754301 7 28.71205939150898 32.73071870560585 20.85734761696065 17.69987428592452 8 26.394854465428818 35.22514463566529 20.00169919764115 18.378301701264746 9 26.73810756034854 16.41278896553189 20.399910716266803 36.449192757852764 10-11 24.945017870969835 26.85206379501001 27.50516444193779 20.697753892082364 12-13 25.79150655300501 24.149599939466086 27.965019285703583 22.09387422182532 14-15 22.65297851709732 25.4717691397262 26.229808515976533 25.645443827199944 16-17 22.13050102751339 28.855763898740054 26.79720966962543 22.21652540412113 18-19 21.91381806106757 27.33378442984997 29.236553913398193 21.515843595684263 20-21 22.787719580047856 26.59048028314398 29.005019270532177 21.616780866275988 22-23 22.43745919128433 26.664071091698453 28.774622483229432 22.123847233787785 24-25 22.256575051571122 26.768217490516648 29.11035011471049 21.864857343201738 26-27 22.118136743930158 27.03649798933874 28.917736176247505 21.927629090483595 28-29 21.936281226312452 27.031370867521943 29.179547349678376 21.852800556487228 30-31 21.977737097334252 26.864371599352708 29.07811700834257 22.07977429497047 32-33 21.849282686370785 26.978479548089286 28.88264290470251 22.28959486083742 34-35 22.40770123317897 26.91924879672973 28.768988797799388 21.904061172291918 36-37 22.355452140460123 26.646358782835726 28.75879914778964 22.239389928914505 38-39 22.13827849900467 26.976073336119548 28.947933680444027 21.93771448443175 40-41 22.190025680632537 26.74311525104878 29.025060545924674 22.041798522394 42-43 22.185649919997694 26.800341118112954 28.705536796840985 22.308472165048364 44-45 22.105912258444548 27.145668968411684 28.463591126183612 22.28482764696015 46-47 22.193894725383633 27.17486682747186 28.136957626207394 22.494280820937114 48-49 22.374955599169883 27.219316110584003 27.94934825365585 22.456380036590268 50-51 22.272630296364078 27.35640020291765 27.847536778824498 22.523432721893773 52-53 22.518180561368627 27.317038988810516 27.788016370707275 22.37676407911358 54-55 22.058932573903245 27.165049931946754 28.024671363608007 22.751346130541997 56-57 22.19568142662987 27.593637377632852 27.766944316197122 22.443736879540158 58-59 22.328356142691476 27.05050487810197 27.892331864545316 22.728807114661233 60-61 22.30097734571981 27.186368933548643 28.081170804605765 22.431482916125777 62-63 22.283133824499107 27.084744206986755 28.037554477660198 22.594567490853937 64-65 22.046246142140664 27.002578417379702 27.994764652090026 22.95641078838961 66-67 21.980996723736553 27.384621949542698 27.70670093072433 22.927680395996415 68-69 22.127089878587654 27.13265258421409 27.865604121778542 22.874653415419708 70-71 21.956285056658633 27.13103995755035 27.86263450653329 23.05004047925772 72-73 22.063408139118774 27.1001637184513 27.715259989565865 23.12116815286407 74-75 21.902659482470884 27.23476670833763 27.565703834740233 23.296869974451248 76-77 21.977549986432457 27.07401236828573 27.831384857104364 23.117052788177453 78-79 21.90903301201275 27.04437953906036 27.71078811614259 23.335799332784298 80-81 21.71956449303218 27.25722815377202 27.676190544823687 23.347016808372118 82-83 21.94733828260336 27.162391439386834 27.522250712042702 23.368019565967106 84-85 21.66714045708037 26.963298658449325 27.556823084510242 23.81273779996006 86-87 21.803670327045896 26.754209360174976 27.779480044279182 23.662640268499953 88-89 21.89177389653406 26.649527523749423 27.60886784596049 23.849830733756036 90-91 21.577798794194962 26.90703440214031 27.74475809886992 23.770408704794804 92-93 21.7028805036778 26.725065467285702 27.70152057057642 23.870533458460073 94-95 21.538119451457288 26.4836515781863 27.667631476016076 24.31059749434034 96-97 21.64913418558828 26.31360858095948 27.639961161145216 24.39729607230702 98-99 21.356060228212105 26.3475101918671 27.551807037902158 24.744622542018636 100-101 21.530806437695393 25.930849676797195 28.13421379270454 24.40413009280287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1173.0 1 795.5 2 582.5 3 776.0 4 698.5 5 667.0 6 1183.0 7 2404.5 8 3681.0 9 4171.5 10 3836.5 11 3514.5 12 3809.0 13 4206.0 14 4599.5 15 4970.0 16 5260.0 17 5738.5 18 6223.0 19 6716.5 20 7558.5 21 8946.0 22 10577.5 23 12894.5 24 15688.5 25 19540.5 26 26017.5 27 33951.5 28 40691.5 29 48147.5 30 57453.5 31 68185.0 32 81439.5 33 93255.0 34 106450.0 35 118272.0 36 130083.5 37 144327.5 38 152028.0 39 153781.0 40 154559.5 41 161458.5 42 171873.0 43 182068.5 44 190806.5 45 198000.0 46 199470.5 47 199936.0 48 218784.0 49 223948.0 50 209374.0 51 199268.0 52 188779.5 53 183545.5 54 177409.5 55 162729.0 56 145225.5 57 128705.5 58 115000.5 59 101096.5 60 85634.5 61 69041.0 62 54165.0 63 41410.5 64 29792.5 65 22296.0 66 16096.0 67 12159.0 68 9101.0 69 6219.5 70 4844.0 71 3882.5 72 3079.0 73 2593.0 74 2076.5 75 1695.5 76 1224.0 77 692.0 78 426.0 79 269.5 80 184.5 81 141.5 82 106.5 83 74.5 84 46.0 85 26.0 86 20.0 87 16.0 88 9.5 89 4.0 90 2.5 91 1.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23346899732357407 2 0.0 3 0.0 4 0.0 5 0.02928081649480939 6 0.0033756381710337248 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002901531686337977 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.6980305806268333E-4 26-27 0.0011473376929637099 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006884026157782259 36-37 0.017153172616292157 38-39 0.013189642404235705 40-41 0.008088256628909458 42-43 0.007898614035031159 44-45 0.0036695841915450884 46-47 5.689277816348974E-4 48-49 0.0017067833449046924 50-51 3.792851877565983E-5 52-53 0.0 54-55 0.0 56-57 0.0011852662117393697 58-59 4.172137065322581E-4 60-61 0.0023136396453152497 62-63 8.344274130645162E-4 64-65 0.0014223194540872435 66-67 0.005727206335124634 68-69 2.5601750173570386E-4 70-71 1.1378555632697947E-4 72-73 0.0 74-75 3.982494471444282E-4 76-77 0.059680524293500736 78-79 0.14153974994106858 80-81 9.482129693914957E-4 82-83 8.249452833706012E-4 84-85 0.0 86-87 0.10601020997796921 88-89 0.3533610451734348 90-91 0.3900853334779674 92-93 4.45660095614003E-4 94-95 2.7498176112353376E-4 96-97 1.327498157148094E-4 98-99 0.0 100-101 0.0013654266759237537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5273077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.7780606143641 #Duplication Level Percentage of deduplicated Percentage of total 1 63.531144969053635 22.730211556027538 2 15.786471811609365 11.296186907254203 3 6.9934750452290695 7.506389222197452 4 3.8256207759000453 5.474931680308898 5 2.4064341856837834 4.304877407993616 6 1.6157070133105225 3.4684118074366235 7 1.1281443788347025 2.8253972577391484 8 0.8281033717234358 2.37023461027843 9 0.626232842006037 2.016485692199774 >10 2.911608559461667 18.004010167786063 >50 0.19108769377503304 4.760412704558945 >100 0.14157008674243782 9.961784804867253 >500 0.010679901385850687 2.5690075966946706 >1k 0.003559963915096184 2.0428403381005293 >5k 5.313378977747217E-5 0.15229102874098854 >10k+ 1.0626757955494434E-4 0.5165272178156783 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14897 0.28251057210050223 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12274 0.23276731972622436 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8011 0.15192268195590544 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.8964259387829914E-5 0.0 5 0.0 0.0 0.0 1.8964259387829914E-5 0.0 6 0.0 0.0 0.0 1.8964259387829914E-5 0.0 7 0.0 0.0 0.0 1.8964259387829914E-5 0.0 8 0.0 0.0 0.0 1.8964259387829914E-5 0.0 9 0.0 0.0 0.0 1.8964259387829914E-5 0.0 10-11 1.8964259387829914E-5 0.0 0.0 1.8964259387829914E-5 0.0 12-13 2.844638908174487E-5 0.0 0.0 3.792851877565983E-5 0.0 14-15 3.792851877565983E-5 0.0 0.0 7.585703755131965E-5 0.0 16-17 3.792851877565983E-5 0.0 0.0 8.533916724523462E-5 0.0 18-19 3.792851877565983E-5 0.0 0.0 1.5171407510263934E-4 0.0 20-21 5.6892778163489744E-5 0.0 0.0 2.5601750173570386E-4 0.0 22-23 5.6892778163489744E-5 0.0 0.0 5.120350034714077E-4 0.0 24-25 7.585703755131965E-5 0.0 0.0 9.861414881671554E-4 0.0 26-27 7.585703755131965E-5 0.0 0.0 0.001782640382456012 0.0 28-29 1.1378555632697949E-4 0.0 0.0 0.004816921884508798 0.0 30-31 1.327498157148094E-4 0.0 0.0 0.015389496493223975 0.0 32-33 1.327498157148094E-4 0.0 0.0 0.036449306543409096 0.0 34-35 1.517140751026393E-4 0.0 0.0 0.07216848910038673 0.0 36-37 1.7067833449046924E-4 0.0 0.0 0.13322392219950516 0.0 38-39 1.801604641843842E-4 0.0 0.0 0.22105688955423938 0.0 40-41 1.8964259387829914E-4 0.0 0.0 0.31788839798849894 0.0 42-43 1.8964259387829914E-4 0.0 0.0 0.41759299172001474 0.0 44-45 1.8964259387829914E-4 0.0 0.0 0.5389547696724322 0.0 46-47 1.991247235722141E-4 0.0 0.0 0.6888862043926156 0.0 48-49 2.0860685326612905E-4 0.0 0.0 0.8559044368212336 0.0 50-51 2.0860685326612905E-4 0.0 0.0 1.0349175633126542 0.0 52-53 2.0860685326612905E-4 1.8964259387829914E-5 0.0 1.2105266052439592 0.0 54-55 2.0860685326612905E-4 1.8964259387829914E-5 0.0 1.4064748153687117 0.0 56-57 2.0860685326612905E-4 1.8964259387829914E-5 0.0 1.6369095311902329 0.0 58-59 2.0860685326612905E-4 3.792851877565983E-5 0.0 1.8945389949739022 0.0 60-61 2.0860685326612905E-4 3.792851877565983E-5 0.0 2.1785951542145128 0.0 62-63 2.0860685326612905E-4 3.792851877565983E-5 0.0 2.4632392054961456 0.0 64-65 2.0860685326612905E-4 3.792851877565983E-5 0.0 2.7562654594271994 0.0 66-67 2.0860685326612905E-4 3.792851877565983E-5 0.0 3.0901312459499453 0.0 68-69 2.0860685326612905E-4 3.792851877565983E-5 0.0 3.4715499128876743 0.0 70-71 2.2757111265395898E-4 3.792851877565983E-5 0.0 3.88594742690084 0.0 72-73 2.465353720417889E-4 3.792851877565983E-5 0.0 4.307058288737297 0.0 74-75 2.465353720417889E-4 3.792851877565983E-5 0.0 4.73537556914113 0.0 76-77 2.465353720417889E-4 3.792851877565983E-5 0.0 5.192708166408342 0.0 78-79 2.465353720417889E-4 3.792851877565983E-5 0.0 5.699556444937178 0.0 80-81 3.603209283687684E-4 3.792851877565983E-5 0.0 6.258622811690405 0.0 82-83 3.603209283687684E-4 3.792851877565983E-5 0.0 6.828034181939691 0.0 84-85 3.603209283687684E-4 3.792851877565983E-5 0.0 7.397445552188978 0.0 86-87 3.603209283687684E-4 5.6892778163489744E-5 0.0 7.993027600393471 0.0 88-89 3.603209283687684E-4 5.6892778163489744E-5 0.0 8.621749691878195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15570 0.0 33.062138 1 GTATCAA 23180 0.0 30.705093 1 GTACATG 25300 0.0 25.612316 1 ATCAACG 28470 0.0 24.722368 3 TCAACGC 28585 0.0 24.606304 4 TACATGG 26120 0.0 24.384604 2 CAACGCA 28940 0.0 24.337263 5 TATCAAC 29560 0.0 23.89103 2 AACGCAG 29620 0.0 23.786894 6 ACATGGG 26660 0.0 22.893763 3 ACGCAGA 33405 0.0 21.005272 7 CGCAGAG 33615 0.0 20.761137 8 GAGTACT 15885 0.0 20.505299 12-13 CATGGGG 10085 0.0 19.436161 4 CGTATAG 270 2.2611857E-8 19.383007 1 CATGGGA 16020 0.0 19.197685 4 GCAGAGT 36220 0.0 18.992895 9 GTACTTT 17240 0.0 18.92118 14-15 AGTACTT 16795 0.0 18.829247 12-13 AGTACAT 19660 0.0 18.612608 2 >>END_MODULE