FastQCFastQC Report
Thu 26 May 2016
SRR938755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307415
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109300.3304695661112984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82880.25058845049683814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57760.1746378969678737No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42080.12722927119820163No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG159450.028.3036611
TACATGG163300.027.2743822
ACATGGG161400.026.3918253
TACCTGG24850.024.7873552
GTATCAA182750.024.281
GGTATCA136250.022.545961
CATGGGG69600.022.1252084
CATGGGA98400.021.8612024
AGTACAT115900.021.340292
ATCAACG213100.020.6559663
GGACCGT3900.020.6405666
GAGTACT100650.020.56100512-13
TCAACGC214900.020.4829524
CAACGCA215700.020.4069845
TATCAAC218350.020.1593152
AACGCAG218700.020.0709636
GTGTAGC15850.020.0389861
GAGTACA151150.019.1002641
GTACTTT108150.019.0256714-15
AGTACTT105400.018.39881912-13
Produced by FastQC (version 0.11.5)