FastQCFastQC Report
Thu 26 May 2016
SRR938755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307415
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110220.3332511946641108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92400.27937225899985335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62450.18881815556862383No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46680.1411374139622636No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG152000.026.7603951
TACATGG156450.025.687892
ACATGGG154100.024.7864233
TACCTGG25500.023.6311072
GGTATCA149800.023.2107541
GTATCAA195050.022.1726761
CGTATAG1656.195913E-520.2065581
GAGTACT111850.020.08742512-13
CATGGGA94400.020.0046834
CATGGGG66600.019.6632564
ATCAACG228150.018.7380713
GTACTTT120450.018.71229614-15
AGTACAT113700.018.6120822
TCAACGC230350.018.5591094
CAACGCA231100.018.5194115
TATCAAC232050.018.4844882
AACGCAG235300.018.1888476
AGTACTT116650.018.04052512-13
CGCGGAA4000.017.32137782-83
GTACCTG36950.017.1441291
Produced by FastQC (version 0.11.5)