##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938755_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3307415 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.977753925648884 31.0 31.0 34.0 30.0 34.0 2 32.19556723302035 34.0 31.0 34.0 30.0 34.0 3 32.2905404976394 34.0 31.0 34.0 30.0 34.0 4 35.92406516871938 37.0 35.0 37.0 35.0 37.0 5 35.83388507338813 37.0 35.0 37.0 35.0 37.0 6 35.862238334167316 37.0 35.0 37.0 35.0 37.0 7 35.79326210953267 37.0 35.0 37.0 33.0 37.0 8 35.756120111930315 37.0 35.0 37.0 33.0 37.0 9 37.400154501325055 39.0 37.0 39.0 34.0 39.0 10-11 37.32540352510949 39.0 37.0 39.0 34.0 39.0 12-13 37.25305684348653 39.0 37.0 39.0 33.0 39.0 14-15 38.474717415262376 40.0 38.0 41.0 33.5 41.0 16-17 38.40599168837294 40.0 38.0 41.0 33.0 41.0 18-19 38.41914395381288 40.0 38.0 41.0 33.5 41.0 20-21 38.49252588501896 40.0 38.0 41.0 34.0 41.0 22-23 38.44644080044385 40.0 38.0 41.0 34.0 41.0 24-25 38.380462385276715 40.0 38.0 41.0 34.0 41.0 26-27 38.223205736201834 40.0 38.0 41.0 33.0 41.0 28-29 38.06400376124556 40.0 37.0 41.0 33.0 41.0 30-31 37.85229612854752 40.0 37.0 41.0 33.0 41.0 32-33 37.64710733306828 40.0 37.0 41.0 32.0 41.0 34-35 37.62945699284789 40.0 37.0 41.0 32.0 41.0 36-37 37.559787628707014 40.0 37.0 41.0 32.0 41.0 38-39 37.46454119002303 40.0 37.0 41.0 32.0 41.0 40-41 37.312366001847366 40.0 36.0 41.0 31.0 41.0 42-43 37.15071377495718 40.0 36.0 41.0 31.0 41.0 44-45 36.91948243567862 40.0 36.0 41.0 31.0 41.0 46-47 36.65441424798521 39.0 36.0 41.0 30.0 41.0 48-49 36.40223543159839 39.0 35.0 41.0 30.0 41.0 50-51 36.181427338268705 39.0 35.0 41.0 29.5 41.0 52-53 35.974929665614994 39.0 35.0 40.5 29.0 41.0 54-55 35.737992359592006 39.0 35.0 40.0 28.0 41.0 56-57 35.48956057827639 38.0 35.0 40.0 28.0 41.0 58-59 35.1905404976394 38.0 34.0 40.0 27.5 41.0 60-61 34.85110592411294 38.0 34.0 40.0 26.5 41.0 62-63 34.4886644101209 37.0 34.0 40.0 26.0 41.0 64-65 34.12714038002488 37.0 33.0 39.5 26.0 41.0 66-67 33.74506767369682 36.0 33.0 39.0 26.0 40.5 68-69 33.302618207875334 35.5 32.5 39.0 25.0 40.0 70-71 32.83343381462562 35.0 32.0 38.0 24.0 40.0 72-73 32.48627054663537 35.0 32.0 37.5 23.0 39.5 74-75 32.06494981730445 35.0 31.5 37.0 22.0 39.0 76-77 30.01512419820313 33.0 29.0 35.0 19.5 37.5 78-79 31.08882314435896 34.0 30.5 36.0 20.0 38.0 80-81 31.1479019113114 34.0 31.0 36.0 20.0 37.0 82-83 30.842614398253623 34.0 31.0 35.0 19.5 37.0 84-85 30.457412208628188 34.0 31.0 35.0 18.0 36.5 86-87 29.92598056185873 34.0 30.0 35.0 12.5 36.0 88-89 29.41363254989168 34.0 29.5 35.0 7.0 36.0 90-91 29.000419209563965 34.0 29.0 35.0 2.0 35.0 92-93 28.72264079348978 34.0 29.0 35.0 2.0 35.0 94-95 28.340111990784344 34.0 29.0 35.0 2.0 35.0 96-97 27.81447399252891 33.5 28.5 35.0 2.0 35.0 98-99 27.074611138910598 33.0 27.0 35.0 2.0 35.0 100-101 23.92291865399413 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0037262723060962344 1101 2 0.06626846742774006 1101 3 0.08467315829670241 1101 4 0.10265531069580902 1101 5 0.037087987155054236 1101 6 0.04957043654056292 1101 7 0.002848087521670095 1101 8 0.031402283102622164 1101 9 0.11271427775315601 1101 10-11 0.08687482442147854 1101 12-13 0.17505397819992652 1101 14-15 0.17831904275787736 1101 16-17 0.09717596197300793 1101 18-19 0.13265914095492803 1101 20-21 0.08763167826440821 1101 22-23 0.12905541581755386 1101 24-25 0.07612307012636421 1101 26-27 0.12927905130495532 1101 28-29 0.16768204192086245 1101 30-31 0.06479844991492456 1101 32-33 0.08904109464320697 1101 34-35 0.140085145866081 1101 36-37 0.15514015696985695 1101 38-39 0.09501741564927357 1101 40-41 0.18683612459127374 1101 42-43 0.16491436359484624 1101 44-45 0.2651594638673558 1101 46-47 0.22916075731858143 1101 48-49 0.221033095184211 1101 50-51 0.27275095766195534 1101 52-53 0.13626163820474346 1101 54-55 0.13130728695059446 1101 56-57 0.14610646687973627 1101 58-59 0.1433813202272063 1101 60-61 0.18109541994618894 1101 62-63 0.03906753228744009 1101 64-65 -0.07621301810362269 1101 66-67 -0.0781527466551708 1101 68-69 -0.07910537686724695 1101 70-71 0.033194814391151795 1101 72-73 0.12370787461100008 1101 74-75 0.044823145078161986 1101 76-77 -0.04176161826850944 1101 78-79 0.05712928739875878 1101 80-81 0.11202903388737084 1101 82-83 0.1552142145934141 1101 84-85 0.12505370584751674 1101 86-87 0.20034387932041398 1101 88-89 0.3434804799189308 1101 90-91 0.540219759550034 1101 92-93 0.4382811389963628 1101 94-95 0.2596231764386765 1101 96-97 0.1765039026120938 1101 98-99 0.35847902526157327 1101 100-101 0.7890071516070662 1102 1 0.14099176780183242 1102 2 0.12266871234696453 1102 3 0.16916250459883742 1102 4 0.059076029631775384 1102 5 0.07436521207030466 1102 6 0.10481173676558342 1102 7 0.043788623479521505 1102 8 0.13934408691155653 1102 9 0.1532362017724438 1102 10-11 0.19707929544689762 1102 12-13 0.15166053968974325 1102 14-15 0.2834135644685034 1102 16-17 0.2466760307003426 1102 18-19 0.20471801961321745 1102 20-21 0.24694081957645864 1102 22-23 0.20395657822263757 1102 24-25 0.12422653215764257 1102 26-27 0.20210863731649198 1102 28-29 0.06285382661110361 1102 30-31 0.08209015082510263 1102 32-33 0.22484034546721432 1102 34-35 0.16077111193688864 1102 36-37 0.0514604571016406 1102 38-39 0.08418964057893419 1102 40-41 0.21384231389614428 1102 42-43 0.2048384078763945 1102 44-45 0.08712351803025342 1102 46-47 -0.002987582219439844 1102 48-49 0.16924565584929496 1102 50-51 0.13045673354548626 1102 52-53 0.09602851316809335 1102 54-55 0.065446268036645 1102 56-57 0.023541745436908457 1102 58-59 0.033146811031677714 1102 60-61 -0.10998708841884763 1102 62-63 -0.004535394824102923 1102 64-65 0.07513682516921705 1102 66-67 0.05942394680074159 1102 68-69 0.1562088416164613 1102 70-71 0.09021062552267267 1102 72-73 0.20320386775941301 1102 74-75 0.3053761549989318 1102 76-77 0.18097174947655503 1102 78-79 0.004929339881446282 1102 80-81 -0.024444802145552558 1102 82-83 0.1415368985543779 1102 84-85 0.09798585418705485 1102 86-87 0.08361444489349878 1102 88-89 0.27025030487089197 1102 90-91 0.39032530166451096 1102 92-93 0.5065736156122433 1102 94-95 0.4947662013833316 1102 96-97 0.5321159459478046 1102 98-99 0.650068001205085 1102 100-101 0.9775125772215034 1103 1 0.09986081582687589 1103 2 0.10516036258694328 1103 3 0.0031688206324247403 1103 4 0.09599117697179338 1103 5 0.046846244644029866 1103 6 0.08487121388531449 1103 7 0.0926537609294158 1103 8 0.10877168784190872 1103 9 0.08208476474975868 1103 10-11 0.07632607774836941 1103 12-13 0.07448209923620652 1103 14-15 0.045210939835968134 1103 16-17 0.004209003442269932 1103 18-19 0.11037226793019528 1103 20-21 0.0878005890798832 1103 22-23 0.042688835693091676 1103 24-25 0.09054016710545199 1103 26-27 0.058459797870611396 1103 28-29 0.17958989461873642 1103 30-31 0.11907691625652461 1103 32-33 0.15355902960414625 1103 34-35 0.11824787016102789 1103 36-37 0.1331943197267762 1103 38-39 0.3280716935399468 1103 40-41 0.24389008202631857 1103 42-43 0.293337338152206 1103 44-45 0.23990281153484005 1103 46-47 0.17612386462516127 1103 48-49 0.2663913338680004 1103 50-51 0.3578603731086858 1103 52-53 0.333550765932209 1103 54-55 0.25748563297742777 1103 56-57 0.334521708286438 1103 58-59 0.14832689074375338 1103 60-61 0.1616617533006277 1103 62-63 0.24378470621425663 1103 64-65 0.22510425598954953 1103 66-67 0.1414005151801021 1103 68-69 0.20174999018875894 1103 70-71 0.2047023758006219 1103 72-73 0.14453984073097814 1103 74-75 0.2317811769049669 1103 76-77 0.23670227674485744 1103 78-79 0.12666076976506346 1103 80-81 0.28986540195463917 1103 82-83 0.1981248535281992 1103 84-85 0.08323309575373727 1103 86-87 0.18842269029572734 1103 88-89 0.3547295276668194 1103 90-91 0.5675860460834166 1103 92-93 0.6177192095081132 1103 94-95 0.4927134336282961 1103 96-97 0.49696624140085177 1103 98-99 0.5825392459729848 1103 100-101 1.187019367298646 1104 1 0.0260217223945709 1104 2 0.02843377475716835 1104 3 0.08458824653369845 1104 4 0.022035320955978932 1104 5 0.12108302823160244 1104 6 0.055297390718330064 1104 7 0.031093775118094413 1104 8 -0.028403615109404257 1104 9 -0.0010970316713354578 1104 10-11 0.022995074012335692 1104 12-13 0.009907914190037559 1104 14-15 0.05980574445701592 1104 16-17 0.12367842446194999 1104 18-19 0.09720905362873111 1104 20-21 0.1155224672027444 1104 22-23 0.07518827308403075 1104 24-25 0.02596230672372002 1104 26-27 0.20640006443233005 1104 28-29 0.18728618863652713 1104 30-31 0.07038192424990797 1104 32-33 0.1307441014088937 1104 34-35 0.15109929161119595 1104 36-37 0.06464233785390405 1104 38-39 0.23953111651603365 1104 40-41 0.2883729510045683 1104 42-43 0.25579227759586587 1104 44-45 0.14061470518456787 1104 46-47 0.09204241202699848 1104 48-49 0.2088871750844561 1104 50-51 0.27537872811141995 1104 52-53 0.22868661010401325 1104 54-55 0.12961034257879334 1104 56-57 0.080683244373148 1104 58-59 0.20965184355809896 1104 60-61 0.19202714008351762 1104 62-63 0.11180072049943135 1104 64-65 0.2563504039551674 1104 66-67 0.15721190299058208 1104 68-69 0.1691671038327769 1104 70-71 0.03946805336851611 1104 72-73 0.024499093510755188 1104 74-75 0.014354356605508656 1104 76-77 -0.1532551138649012 1104 78-79 0.01832705005029922 1104 80-81 0.09019026392222784 1104 82-83 0.03735917369855457 1104 84-85 0.21954620267885971 1104 86-87 0.36526829303003083 1104 88-89 0.4530243039029642 1104 90-91 0.4526239529289242 1104 92-93 0.3734432148021014 1104 94-95 0.4044615118047581 1104 96-97 0.3886786047396207 1104 98-99 0.4819578927031216 1104 100-101 0.9223166114755017 1105 1 -0.02326878557095924 1105 2 -0.06936637110988642 1105 3 0.028262437813580732 1105 4 -0.009433704799398868 1105 5 -0.007838921923777775 1105 6 -0.07017371515083681 1105 7 -0.020005839902935918 1105 8 -0.013226765249690686 1105 9 0.07388427160628908 1105 10-11 0.06522427743693271 1105 12-13 -0.07293981813322148 1105 14-15 0.07031959535017052 1105 16-17 0.07908443670755361 1105 18-19 -0.005964798325379661 1105 20-21 0.08380290678586988 1105 22-23 -0.06732216838177862 1105 24-25 -0.08187033631152474 1105 26-27 -0.07212616010252049 1105 28-29 0.059684616192292594 1105 30-31 -0.08290032566954864 1105 32-33 0.011456317694580775 1105 34-35 0.07900251743289743 1105 36-37 0.07107926784500052 1105 38-39 0.033843956060472635 1105 40-41 0.06488742806889292 1105 42-43 0.03554947409646303 1105 44-45 0.00956559802693846 1105 46-47 0.059449313507542456 1105 48-49 0.17803467546340102 1105 50-51 0.19930980465020554 1105 52-53 0.0571458725250622 1105 54-55 0.1508077781704955 1105 56-57 0.11985799577458778 1105 58-59 0.14860355812011505 1105 60-61 0.06274714143003735 1105 62-63 -0.12426103726385662 1105 64-65 0.1050930925178406 1105 66-67 0.12903008678591021 1105 68-69 0.12995670360425038 1105 70-71 -0.014779390908437051 1105 72-73 -0.2916857198430449 1105 74-75 -0.15021088892744672 1105 76-77 0.006042284631206485 1105 78-79 -0.051835704338831334 1105 80-81 0.12927915554913127 1105 82-83 0.04541840900000693 1105 84-85 0.1837299584823171 1105 86-87 0.0865877005635447 1105 88-89 0.193132534498492 1105 90-91 0.26046519139754665 1105 92-93 0.09799460930006276 1105 94-95 0.08110159188507282 1105 96-97 0.12419319555869635 1105 98-99 0.1740841258617678 1105 100-101 0.5880453136889336 1106 1 -0.10677252979141372 1106 2 -0.15610880990749365 1106 3 -0.0839394826330988 1106 4 -0.0962926570872682 1106 5 -0.09584731546050307 1106 6 -0.08910045184860849 1106 7 -0.057414564661357304 1106 8 -0.009605621085313487 1106 9 -0.16669963123230502 1106 10-11 -0.05937472593044646 1106 12-13 -0.019169270384765014 1106 14-15 0.001984766205552546 1106 16-17 -0.12005200334782984 1106 18-19 -0.16711589073684507 1106 20-21 -0.1226621366941103 1106 22-23 -0.07846813419966736 1106 24-25 -0.047316001838481725 1106 26-27 -0.1374776732180365 1106 28-29 -0.07377026845269086 1106 30-31 -0.07530616660019263 1106 32-33 -0.09243186587817576 1106 34-35 -0.023539863992510845 1106 36-37 -0.08533552740893668 1106 38-39 -0.044622981310624255 1106 40-41 0.03939560798171016 1106 42-43 0.06758003125731449 1106 44-45 -0.05774272349017906 1106 46-47 -0.015828768526326087 1106 48-49 -0.07915320819887484 1106 50-51 -0.06123219327937335 1106 52-53 0.03998429142652782 1106 54-55 0.028659172394696952 1106 56-57 -0.019903264754901784 1106 58-59 -0.03375265662385374 1106 60-61 -0.07375630679859313 1106 62-63 0.0028590802502179713 1106 64-65 0.054309237311862546 1106 66-67 -0.01744491883527388 1106 68-69 -0.040163930183481966 1106 70-71 -0.0938550518205119 1106 72-73 0.0794138365116197 1106 74-75 0.2006526519945666 1106 76-77 0.15705727406795233 1106 78-79 0.14679425870784613 1106 80-81 -0.012333908571143581 1106 82-83 0.15910532954750778 1106 84-85 -0.023111970471276777 1106 86-87 0.03600486679567183 1106 88-89 0.23385219219802877 1106 90-91 0.31151807387471564 1106 92-93 0.4225779855927918 1106 94-95 0.38894782907952674 1106 96-97 0.38639120685888884 1106 98-99 0.4524830764223857 1106 100-101 0.8119437238171798 1107 1 -0.16066000171006678 1107 2 -0.12012930811798128 1107 3 -0.13624829956617646 1107 4 -0.08116373156359913 1107 5 -0.12889077984950603 1107 6 -0.13628499767680324 1107 7 -0.056695006530858905 1107 8 -0.0902569394306667 1107 9 -0.02119274062803811 1107 10-11 -0.028105336428787098 1107 12-13 -0.10622313589833254 1107 14-15 -0.17933440493254693 1107 16-17 -0.06428835961354906 1107 18-19 -0.08927908817240393 1107 20-21 -0.06505497714701391 1107 22-23 -0.19775652423996348 1107 24-25 -0.24923233678396883 1107 26-27 -0.17299004402507023 1107 28-29 -0.15805423867191593 1107 30-31 -0.17093077834096704 1107 32-33 -0.10712143678955499 1107 34-35 -0.06635408887225935 1107 36-37 -0.029379607382360007 1107 38-39 -0.0353145190893045 1107 40-41 -0.14303752697579597 1107 42-43 -0.09966984534428747 1107 44-45 -0.16844765210678503 1107 46-47 -0.11309176383249309 1107 48-49 -0.33814693411741814 1107 50-51 -0.24043881193796324 1107 52-53 -0.2557993775692182 1107 54-55 -0.2821707498877757 1107 56-57 -0.2769926671312888 1107 58-59 -0.17851939141770146 1107 60-61 -0.2875781945783018 1107 62-63 -0.3186174965749018 1107 64-65 -0.28954763368003 1107 66-67 -0.32655148625772057 1107 68-69 -0.16535925624616965 1107 70-71 -0.24536726873144232 1107 72-73 -0.2539620997678824 1107 74-75 -0.30471536614265204 1107 76-77 -0.28682676334006274 1107 78-79 -0.2474575810074846 1107 80-81 -0.22386973539673605 1107 82-83 -0.2250822210083001 1107 84-85 -0.23020099749130196 1107 86-87 -0.28322370082571524 1107 88-89 -0.03729252269067018 1107 90-91 0.19974314505166646 1107 92-93 0.1945174706414079 1107 94-95 0.17874142062049714 1107 96-97 0.2185712108752007 1107 98-99 0.13837155429606796 1107 100-101 0.46824454941348037 1108 1 -0.2026957680672865 1108 2 -0.20981135748503732 1108 3 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9 2160.0 10 3410.0 11 5174.0 12 7162.0 13 10027.0 14 12110.0 15 15375.0 16 19351.0 17 25371.0 18 24285.0 19 17796.0 20 16806.0 21 18095.0 22 19281.0 23 21950.0 24 25666.0 25 30774.0 26 38093.0 27 47119.0 28 58133.0 29 70653.0 30 86566.0 31 105123.0 32 129023.0 33 161858.0 34 214550.0 35 296430.0 36 445104.0 37 696086.0 38 615687.0 39 66268.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.243819092183 19.625510997234112 13.82695006014053 23.30371985044235 2 16.098614779215794 21.11153272268524 38.85167116917593 23.93818132892304 3 19.807825749112222 24.782012538493053 29.661533251799366 25.748628460595356 4 13.143073971666693 16.50201138955952 36.55138529637194 33.803529342401845 5 13.452318502516317 38.00478621521641 34.15117244131746 14.391722840949805 6 32.31584182813466 35.9735624347111 17.196360299508832 14.514235437645413 7 28.844822920619272 32.66345469195732 20.56164708692438 17.93007530049903 8 26.527605395754687 35.25620461901515 19.627352479202035 18.58883750602812 9 26.659007109782113 16.384910874504712 20.274534644125396 36.68154737158778 10-11 25.209325107372372 26.672869899906726 27.143282593808156 20.974522398912747 12-13 25.96550478243583 23.906736832239076 27.822332546717 22.305425838608098 14-15 22.666255468780708 25.406314911063337 26.146440870651055 25.780988749504903 16-17 22.139331236189875 28.73824287436853 26.799307362134588 22.32311852730701 18-19 21.958773500061906 27.331192384134845 29.04906147904487 21.660972636758384 20-21 22.819183987976107 26.412882328477426 28.988776580619092 21.77915710292738 22-23 22.37916923035059 26.515178772545934 28.78213045535562 22.32352154174786 24-25 22.380817042917204 26.63026865391854 29.0137463850167 21.975167918147555 26-27 22.204334780941164 26.705862486679532 29.038004202985988 22.051798529393313 28-29 21.931765442195793 26.950654816525898 29.127551274938284 21.99002846634003 30-31 22.093311543909667 26.68794209374995 29.02529619052946 22.193450171810916 32-33 21.907532015184064 26.879587230510836 28.987865145438356 22.225015608866745 34-35 22.42143117184391 26.809359922882564 28.796757584443956 21.97245132082957 36-37 22.387419922618893 26.60812103978993 28.866957504801732 22.137501532789447 38-39 22.18990601370757 26.80062278543173 29.029402995635422 21.98006820522528 40-41 22.27920319235885 26.692145828413434 29.01762455675542 22.0110264224723 42-43 22.08507190111631 26.851483980987627 28.6606980878778 22.40274603001826 44-45 22.232396526344417 26.91662800279922 28.514961291534934 22.33601417932143 46-47 22.28685416767452 27.10604530501669 28.154192467708384 22.452908059600407 48-49 22.403825948663837 27.134453946662273 28.08690472771031 22.374815376963582 50-51 22.26311535855495 27.255373630231112 28.08755273400474 22.393958277209205 52-53 22.46369143273523 27.37547299023558 27.84352734688571 22.31730823014348 54-55 22.134627798446825 27.09259346045174 28.04052409510146 22.732254645999973 56-57 22.27054965887256 27.460418483921735 27.782150108166043 22.486881749039657 58-59 22.40052947942938 27.094353131516165 27.850294468509173 22.654822920545282 60-61 22.366990446254665 27.087897601555788 28.175914330134237 22.36919762205531 62-63 22.238409755050395 27.09661472781613 28.150050719368448 22.51492479776502 64-65 22.023408008973774 27.10533755213664 28.087781545406305 22.783472893483278 66-67 22.010282152210138 27.3376990226256 27.716019455269976 22.93599936989428 68-69 22.006276549442834 27.154564905474572 27.956813687407035 22.88234485767556 70-71 22.01440425959717 27.09563986932156 27.885228528537155 23.00472734254412 72-73 22.0451168057229 27.013422869521968 27.780683706157227 23.160776618597907 74-75 21.892006294946356 27.32774689598977 27.592394664715496 23.18785214434838 76-77 21.990233113594527 27.024861495551455 27.83193401419879 23.15297137665523 78-79 21.926985561982633 27.08593290286403 27.811818664514774 23.175262870638562 80-81 21.837529260020062 27.252877928194053 27.659540051532712 23.250052760253176 82-83 21.949528559312938 27.11791535081022 27.663099429614974 23.269456660261866 84-85 21.802117363560363 26.95627854381745 27.609885665995954 23.631718426626232 86-87 21.79034849762379 26.887930769525425 27.78389534821223 23.537825384638563 88-89 21.866527447902552 26.827454834163788 27.606358944459263 23.699658773474397 90-91 21.662179312343966 26.854416419305018 27.73700439800647 23.746399870344543 92-93 21.720255849356672 26.808292881298534 27.7057460282426 23.76570524110219 94-95 21.579491043378763 26.609131274332647 27.73708216405357 24.07429551823502 96-97 21.54299401284508 26.408018470019172 27.804804599605614 24.24418291753013 98-99 21.456240933226827 26.481795703339717 27.595765382296577 24.46619798113688 100-101 21.51267735262264 26.130950833193744 28.019114142433587 24.337257671750024 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1530.0 1 1057.0 2 732.0 3 938.0 4 864.0 5 810.5 6 1383.5 7 2679.5 8 3938.5 9 4379.5 10 3830.5 11 3122.5 12 2994.0 13 3134.5 14 3339.0 15 3573.0 16 3755.0 17 3987.5 18 4329.0 19 4756.5 20 5478.0 21 6353.0 22 7369.0 23 8923.5 24 10918.5 25 13381.5 26 17104.0 27 21724.0 28 25948.5 29 30384.0 30 35715.0 31 42063.5 32 49489.0 33 56369.0 34 63391.5 35 70359.0 36 78351.5 37 86394.5 38 90363.0 39 92545.0 40 94794.0 41 98569.0 42 103763.0 43 109171.5 44 114534.0 45 119194.0 46 121325.0 47 125217.5 48 139728.5 49 140439.5 50 127884.0 51 122185.5 52 117460.5 53 115640.0 54 112271.0 55 104378.0 56 95017.0 57 84847.0 58 76177.0 59 67452.5 60 56650.0 61 46002.0 62 37283.5 63 29247.5 64 21801.0 65 16242.0 66 11813.5 67 9166.0 68 6567.0 69 4041.0 70 2796.5 71 2095.0 72 1702.0 73 1451.5 74 1191.5 75 922.0 76 598.5 77 328.0 78 182.5 79 119.0 80 62.5 81 35.0 82 26.5 83 13.0 84 12.5 85 11.5 86 4.5 87 2.0 88 1.0 89 0.5 90 1.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3819296943383277 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.5117546482676047E-4 16-17 0.0017838704849557736 18-19 1.0582282537873232E-4 20-21 1.5117546482676048E-5 22-23 0.0 24-25 0.0 26-27 1.3605791834408443E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007589008334303376 36-37 0.016190892282946047 38-39 0.010189226329323656 40-41 0.008103004914714362 42-43 0.002872333831708449 44-45 0.0019199284032998581 46-47 0.0016629301130943652 48-49 0.0 50-51 4.232913015149293E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.686439409629575E-4 60-61 6.349369522723941E-4 62-63 0.0 64-65 0.0 66-67 0.0010128756143392953 68-69 7.407597776511263E-4 70-71 0.0018141055779211258 72-73 0.0 74-75 0.0 76-77 0.09641971146650782 78-79 0.035632057059667446 80-81 3.0235092965352096E-5 82-83 0.0 84-85 0.0 86-87 0.15106964200138176 88-89 0.33030327309998897 90-91 0.2855251004183025 92-93 0.0 94-95 4.535263944802815E-5 96-97 3.0235092965352096E-5 98-99 2.7211583668816885E-4 100-101 6.349369522723941E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3307415.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.16068863481777 #Duplication Level Percentage of deduplicated Percentage of total 1 71.62297433308315 35.210347402882455 2 14.968596258607135 14.717329999393671 3 5.498979123500783 8.110008014993554 4 2.621111184855478 5.154225233436737 5 1.4712033526517627 3.616268496910665 6 0.8774423979196219 2.5881403511468637 7 0.589586030013953 2.0289118671467987 8 0.40106810369602425 1.577342733372564 9 0.3027727091890807 1.3396063395208164 >10 1.4578448593252085 12.941312146971104 >50 0.1191509631707201 4.044277900704213 >100 0.06453120477009223 5.77581613065042 >500 0.003385342299425014 1.118623171917477 >1k 0.001169481883443076 0.9757088329276268 >5k 1.2310335615190273E-4 0.46856415809844326 >10k+ 6.155167807595137E-5 0.3335172199264476 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11022 0.3332511946641108 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9240 0.27937225899985335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6245 0.18881815556862383 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4668 0.1411374139622636 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.0235092965352096E-5 0.0 0.0 3.0235092965352096E-5 0.0 3 3.0235092965352096E-5 0.0 0.0 3.0235092965352096E-5 0.0 4 3.0235092965352096E-5 0.0 0.0 3.0235092965352096E-5 0.0 5 3.0235092965352096E-5 0.0 0.0 3.0235092965352096E-5 0.0 6 6.047018593070419E-5 0.0 0.0 3.0235092965352096E-5 3.0235092965352096E-5 7 9.070527889605628E-5 0.0 0.0 6.047018593070419E-5 3.0235092965352096E-5 8 9.070527889605628E-5 0.0 0.0 6.047018593070419E-5 3.0235092965352096E-5 9 9.070527889605628E-5 0.0 0.0 9.070527889605628E-5 3.0235092965352096E-5 10-11 9.070527889605628E-5 0.0 0.0 1.2094037186140838E-4 3.0235092965352096E-5 12-13 1.5117546482676047E-4 0.0 0.0 1.6629301130943652E-4 9.07052788960563E-5 14-15 1.5117546482676047E-4 0.0 0.0 1.9652810427478863E-4 1.3605791834408443E-4 16-17 1.5117546482676047E-4 0.0 0.0 2.1164565075746468E-4 1.8141055779211256E-4 18-19 1.6629301130943652E-4 0.0 0.0 2.2676319724014072E-4 1.8141055779211256E-4 20-21 1.8141055779211256E-4 0.0 0.0 3.17468476136197E-4 2.4188074372281677E-4 22-23 1.8141055779211256E-4 0.0 0.0 6.34936952272394E-4 2.4188074372281677E-4 24-25 3.3258602261887303E-4 0.0 0.0 0.0010582282537873232 2.4188074372281677E-4 26-27 3.3258602261887303E-4 0.0 0.0 0.001632695020129013 2.4188074372281677E-4 28-29 5.291141268936616E-4 0.0 0.0 0.004368970933493378 2.4188074372281677E-4 30-31 5.744667663416898E-4 0.0 0.0 0.014724490274126471 2.4188074372281677E-4 32-33 5.744667663416898E-4 0.0 0.0 0.03613093609359576 2.7211583668816885E-4 34-35 5.895843128243658E-4 0.0 0.0 0.07146064222360968 2.7211583668816885E-4 36-37 6.34936952272394E-4 0.0 0.0 0.13138659648093753 2.7211583668816885E-4 38-39 6.954071382030982E-4 0.0 0.0 0.21557621284296044 2.872333831708449E-4 40-41 7.256422311684502E-4 0.0 0.0 0.3091538255707252 3.0235092965352094E-4 42-43 7.709948706164784E-4 0.0 0.0 0.4029279664027647 3.0235092965352094E-4 44-45 8.768176959952108E-4 0.0 0.0 0.5170352072540035 3.0235092965352094E-4 46-47 9.82640521373943E-4 0.0 0.0 0.656449220917242 3.0235092965352094E-4 48-49 9.97758067856619E-4 0.0 0.0 0.8110714863420526 3.0235092965352094E-4 50-51 9.97758067856619E-4 0.0 0.0 0.9779691995107962 3.0235092965352094E-4 52-53 9.97758067856619E-4 0.0 0.0 1.1403014136417715 3.0235092965352094E-4 54-55 0.0010279931608219712 0.0 0.0 1.3166627109086704 3.628211155842251E-4 56-57 0.0010884633467526754 0.0 0.0 1.525360440102013 3.628211155842251E-4 58-59 0.0010884633467526754 0.0 0.0 1.7636129726689878 3.628211155842251E-4 60-61 0.0010884633467526754 0.0 0.0 2.024133651204944 3.628211155842251E-4 62-63 0.0011186984397180276 0.0 0.0 2.278879426984518 4.2329130151492935E-4 64-65 0.0011186984397180276 0.0 0.0 2.542091028794391 4.2329130151492935E-4 66-67 0.001269873904544788 0.0 0.0 2.8421289738360622 4.2329130151492935E-4 68-69 0.001269873904544788 0.0 0.0 3.1822737696962733 4.2329130151492935E-4 70-71 0.0013001089975101401 0.0 0.0 3.553742121868589 4.2329130151492935E-4 72-73 0.0013605791834408443 0.0 0.0 3.927205990176618 4.2329130151492935E-4 74-75 0.0014664020088195765 0.0 0.0 4.305658648823931 4.2329130151492935E-4 76-77 0.0015117546482676047 0.0 0.0 4.715419746236865 4.2329130151492935E-4 78-79 0.0015419897412329569 0.0 0.0 5.1832171045967925 4.2329130151492935E-4 80-81 0.0017687529384730976 0.0 0.0 5.693918059874555 4.2329130151492935E-4 82-83 0.0017838704849557736 0.0 0.0 6.205601655673691 4.8376148744563353E-4 84-85 0.001889693310334506 0.0 0.0 6.709560185220179 4.8376148744563353E-4 86-87 0.0020106336821959143 0.0 0.0 7.239430189437975 5.593492198590138E-4 88-89 0.0020257512286785905 0.0 0.0 7.8091802812770705 6.047018593070419E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15200 0.0 26.760395 1 TACATGG 15645 0.0 25.68789 2 ACATGGG 15410 0.0 24.786423 3 TACCTGG 2550 0.0 23.631107 2 GGTATCA 14980 0.0 23.210754 1 GTATCAA 19505 0.0 22.172676 1 CGTATAG 165 6.195913E-5 20.206558 1 GAGTACT 11185 0.0 20.087425 12-13 CATGGGA 9440 0.0 20.004683 4 CATGGGG 6660 0.0 19.663256 4 ATCAACG 22815 0.0 18.738071 3 GTACTTT 12045 0.0 18.712296 14-15 AGTACAT 11370 0.0 18.612082 2 TCAACGC 23035 0.0 18.559109 4 CAACGCA 23110 0.0 18.519411 5 TATCAAC 23205 0.0 18.484488 2 AACGCAG 23530 0.0 18.188847 6 AGTACTT 11665 0.0 18.040525 12-13 CGCGGAA 400 0.0 17.321377 82-83 GTACCTG 3695 0.0 17.144129 1 >>END_MODULE