FastQCFastQC Report
Thu 26 May 2016
SRR938754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310440
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107750.3254854339604403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86450.26114353379007016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54960.1660202269184761No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41510.1253911866700499No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG152300.028.6749061
TACATGG156450.027.3526692
ACATGGG158450.026.0492843
TACCTGG25400.024.0948772
GTATAGG9300.024.0160351
GTATCAA190400.023.6607111
GGTATCA143150.023.005321
CATGGGA97750.022.3259434
CATGGGG63450.021.1603854
GAGTACT105600.020.58720212-13
ATCAACG221300.020.0661093
TATCAAC223950.019.9769572
TCAACGC223450.019.8730354
CAACGCA223600.019.8384865
AGTACAT114000.019.6012952
AACGCAG227650.019.5269376
GTACCTG35200.019.3054431
GTACTTT112950.019.12174414-15
AGTACTT110450.018.71622312-13
ATGGGAG43900.018.696075
Produced by FastQC (version 0.11.5)