##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938754_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3310440 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.239899832046497 31.0 31.0 34.0 28.0 34.0 2 31.296437331593385 33.0 31.0 34.0 28.0 34.0 3 31.31228749048465 33.0 31.0 34.0 27.0 34.0 4 34.85935917884027 37.0 35.0 37.0 32.0 37.0 5 34.892619712183276 37.0 35.0 37.0 32.0 37.0 6 34.938781551697055 37.0 35.0 37.0 32.0 37.0 7 34.82955015043318 37.0 35.0 37.0 32.0 37.0 8 34.79237321926995 37.0 35.0 37.0 32.0 37.0 9 36.31526504029676 39.0 37.0 39.0 32.0 39.0 10-11 36.178248057660014 39.0 37.0 39.0 32.0 39.0 12-13 36.05538719928469 39.0 35.0 39.0 30.0 39.0 14-15 37.15752226290161 40.0 37.0 41.0 31.0 41.0 16-17 37.03307385121011 40.0 37.0 41.0 31.0 41.0 18-19 36.95683020988147 40.0 36.0 41.0 30.5 41.0 20-21 36.832563496091154 39.5 36.0 41.0 30.0 41.0 22-23 36.8406226966808 40.0 36.0 41.0 30.0 41.0 24-25 36.77332695955825 40.0 36.0 41.0 30.0 41.0 26-27 36.627944019526105 39.5 36.0 41.0 30.0 41.0 28-29 36.445303947511505 39.0 36.0 41.0 29.5 41.0 30-31 36.21903478087505 39.0 35.5 41.0 29.0 41.0 32-33 36.03382918886915 39.0 35.0 41.0 28.0 41.0 34-35 35.740867377146245 39.0 35.0 41.0 27.0 41.0 36-37 35.52486467055739 38.5 35.0 40.0 26.0 41.0 38-39 35.3500570921086 38.0 35.0 40.0 25.5 41.0 40-41 35.23640845325697 38.5 35.0 40.0 25.0 41.0 42-43 35.23903363299138 39.0 35.0 41.0 25.0 41.0 44-45 35.07881399451432 39.0 34.5 41.0 24.5 41.0 46-47 34.78769936926813 38.5 34.0 40.5 24.0 41.0 48-49 34.51801180507727 38.0 34.0 40.0 23.0 41.0 50-51 33.39819540604874 37.5 32.5 39.5 21.0 40.5 52-53 33.48056557436474 37.5 33.0 39.5 21.0 40.5 54-55 33.97435824240887 38.0 33.0 40.0 21.0 41.0 56-57 33.864430710117084 38.0 33.0 40.0 20.0 41.0 58-59 33.65354983023404 38.0 33.0 40.0 19.5 41.0 60-61 33.33226580152487 37.5 33.0 40.0 18.0 41.0 62-63 32.92612311354382 37.0 32.0 40.0 15.0 41.0 64-65 32.52059001220381 36.0 31.5 39.5 13.0 41.0 66-67 32.12348267903964 36.0 31.0 39.0 9.0 41.0 68-69 31.68885269027682 35.0 31.0 39.0 7.5 40.0 70-71 31.25883281376494 35.0 30.5 38.0 6.5 40.0 72-73 30.822517701574412 35.0 30.0 37.5 2.0 40.0 74-75 30.33748610456616 35.0 29.5 37.0 2.0 39.0 76-77 29.858926305868707 34.0 29.0 36.0 2.0 39.0 78-79 29.41051763511799 34.0 29.0 36.0 2.0 38.5 80-81 28.920679275262504 34.0 29.0 35.5 2.0 37.0 82-83 28.49535016493276 34.0 28.0 35.0 2.0 37.0 84-85 28.12792121289013 34.0 27.0 35.0 2.0 36.5 86-87 27.676166159181257 34.0 26.0 35.0 2.0 36.0 88-89 27.197816151327316 33.5 25.5 35.0 2.0 36.0 90-91 26.71600225347688 33.0 25.0 35.0 2.0 35.0 92-93 26.21182924324259 33.0 24.5 35.0 2.0 35.0 94-95 25.661088405166684 32.5 21.5 35.0 2.0 35.0 96-97 25.002474746559372 32.0 18.0 35.0 2.0 35.0 98-99 24.17660930269088 32.0 2.0 34.5 2.0 35.0 100-101 21.74539517405541 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3077969801856213 1101 2 0.40805890565144054 1101 3 0.44757415919205457 1101 4 0.2092139695094275 1101 5 0.25054403563733985 1101 6 0.3818854457269225 1101 7 0.4234053668688418 1101 8 0.46443654839992377 1101 9 0.5555638262166411 1101 10-11 0.4996168152827636 1101 12-13 0.4479134458725653 1101 14-15 0.5835349952517461 1101 16-17 0.6618415214042059 1101 18-19 0.633907671500836 1101 20-21 0.6985117335356392 1101 22-23 0.6090623182326809 1101 24-25 0.6832820220117028 1101 26-27 0.56343360432124 1101 28-29 0.687445979332864 1101 30-31 0.7971038696638857 1101 32-33 0.5698712642399215 1101 34-35 0.6489784136735963 1101 36-37 0.7456710919815208 1101 38-39 0.691914451095208 1101 40-41 0.7808419291472788 1101 42-43 0.7588755958176918 1101 44-45 0.897662395392878 1101 46-47 0.770974901624264 1101 48-49 0.6726117074135729 1101 50-51 0.8073766049429665 1101 52-53 0.8320984422188928 1101 54-55 0.7800078727798123 1101 56-57 0.7705045484953317 1101 58-59 0.7587368371814307 1101 60-61 0.692884107759383 1101 62-63 0.5673595183320685 1101 64-65 0.49324756732853814 1101 66-67 0.4075139774601908 1101 68-69 0.5094493977900498 1101 70-71 0.5023435228617998 1101 72-73 0.4531631050929157 1101 74-75 0.38923269542467054 1101 76-77 0.3269148042778305 1101 78-79 0.1331239371977091 1101 80-81 0.09447813651797787 1101 82-83 0.29609235717670046 1101 84-85 0.25419416994644806 1101 86-87 0.03018888002251785 1101 88-89 0.12493092233418324 1101 90-91 -0.05132285572505779 1101 92-93 -0.14799067254109133 1101 94-95 -0.22005056063368755 1101 96-97 -0.1606393524307954 1101 98-99 -0.18935677871640166 1101 100-101 0.0055950677940970195 1102 1 0.18615420136790206 1102 2 0.16996507846359776 1102 3 0.21658142892537668 1102 4 0.27723923450948007 1102 5 0.3564572935781172 1102 6 0.3710835782657185 1102 7 0.45170462217345886 1102 8 0.44832221901344127 1102 9 0.5276903616662878 1102 10-11 0.5066257944556583 1102 12-13 0.4916481006063762 1102 14-15 0.5289513065543545 1102 16-17 0.7764127400537149 1102 18-19 0.8194177250731229 1102 20-21 0.8177367906708 1102 22-23 0.7804421276467792 1102 24-25 0.6837582560018589 1102 26-27 0.6909134916385185 1102 28-29 0.8301519704939864 1102 30-31 0.9313620264228177 1102 32-33 0.9024576869330119 1102 34-35 0.8319201542744494 1102 36-37 0.778890155001875 1102 38-39 0.8791585428215924 1102 40-41 0.9394348628483513 1102 42-43 1.1310793421855934 1102 44-45 1.0858252647407411 1102 46-47 0.995654243250037 1102 48-49 1.0164777545468482 1102 50-51 1.1123503633840173 1102 52-53 0.9453793201250278 1102 54-55 0.9004442598441855 1102 56-57 1.009608486482918 1102 58-59 0.8547065933924571 1102 60-61 0.8803376796758471 1102 62-63 0.7413826363793987 1102 64-65 0.7267700281831111 1102 66-67 0.7329127236250343 1102 68-69 0.87124268482453 1102 70-71 0.7660187164198362 1102 72-73 0.7211033134747069 1102 74-75 0.5877090143329902 1102 76-77 0.604691535962278 1102 78-79 0.5527813385541869 1102 80-81 0.7149299842264796 1102 82-83 0.5363581048811383 1102 84-85 0.4383917763618328 1102 86-87 0.4932191570403397 1102 88-89 0.5590151353089396 1102 90-91 0.36750913183997014 1102 92-93 0.3585015057575198 1102 94-95 0.3944647227899196 1102 96-97 0.39933562440131354 1102 98-99 0.40363558049481796 1102 100-101 0.5529082386014466 1103 1 0.22511510412820357 1103 2 0.3142631032890648 1103 3 0.3324391262772366 1103 4 0.4085339165960207 1103 5 0.44172583871392135 1103 6 0.3084121911394604 1103 7 0.3232183706776155 1103 8 0.35882005307757936 1103 9 0.43699704140363593 1103 10-11 0.4126752908369369 1103 12-13 0.5247523106027998 1103 14-15 0.5570110497330916 1103 16-17 0.6452996565647311 1103 18-19 0.6125816142196001 1103 20-21 0.5598836172767037 1103 22-23 0.557188454828939 1103 24-25 0.5348841121095305 1103 26-27 0.6757751234426763 1103 28-29 0.6561656108117475 1103 30-31 0.8041538647525996 1103 32-33 0.6853761585462195 1103 34-35 0.7367782203432256 1103 36-37 0.7358419038681205 1103 38-39 0.7862777598170183 1103 40-41 0.8225198642393181 1103 42-43 0.8701941897752477 1103 44-45 0.8910737401956723 1103 46-47 0.8805720657448646 1103 48-49 0.8755566908758396 1103 50-51 0.8504026659880068 1103 52-53 0.8814397786587307 1103 54-55 0.8681401324226883 1103 56-57 0.8560022915972638 1103 58-59 0.8985460955583946 1103 60-61 0.8171896652274313 1103 62-63 0.7755278644760608 1103 64-65 0.6659620965745745 1103 66-67 0.580337335368327 1103 68-69 0.7054635882030311 1103 70-71 0.6437269641975973 1103 72-73 0.586822190014157 1103 74-75 0.4545688157999095 1103 76-77 0.373620843878431 1103 78-79 0.36648022048087725 1103 80-81 0.4106996200578159 1103 82-83 0.37031102613468647 1103 84-85 0.34746359225949064 1103 86-87 0.3911179674315335 1103 88-89 0.40440297250828294 1103 90-91 0.3306313692230063 1103 92-93 0.13133656344957245 1103 94-95 0.22618188380219095 1103 96-97 0.38278062155314174 1103 98-99 0.46835008983638104 1103 100-101 0.7587830912250766 1104 1 0.23145535037518528 1104 2 0.20828949335306746 1104 3 0.2410335507357324 1104 4 0.2945230488326729 1104 5 0.33486470194759477 1104 6 0.36192160893477876 1104 7 0.35474360356595014 1104 8 0.3739333055916134 1104 9 0.5152694803854914 1104 10-11 0.4567636839881857 1104 12-13 0.4961652274820665 1104 14-15 0.5859617013037237 1104 16-17 0.6484038475859037 1104 18-19 0.6276717232201676 1104 20-21 0.66021639947148 1104 22-23 0.6210833897364623 1104 24-25 0.5754159392845466 1104 26-27 0.5848770905336735 1104 28-29 0.6296493339249025 1104 30-31 0.6564309711890743 1104 32-33 0.6108007612851267 1104 34-35 0.5663458887472714 1104 36-37 0.6473712487667527 1104 38-39 0.7096355929813498 1104 40-41 0.6657798810748758 1104 42-43 0.6351018417743077 1104 44-45 0.703423172802843 1104 46-47 0.9375815362858617 1104 48-49 0.9143236905938181 1104 50-51 0.9832986407389654 1104 52-53 0.9964985631829322 1104 54-55 0.9048764233894033 1104 56-57 0.8064146228918148 1104 58-59 0.893068348853923 1104 60-61 0.8117094965273708 1104 62-63 0.965322705302512 1104 64-65 0.8812842327531598 1104 66-67 0.9023176989044899 1104 68-69 0.825036703406063 1104 70-71 0.8979234752322043 1104 72-73 0.7991413207653189 1104 74-75 0.7629006959358868 1104 76-77 0.7101802753445661 1104 78-79 0.618097575317222 1104 80-81 0.581725340574593 1104 82-83 0.5696891653100913 1104 84-85 0.6806298078967572 1104 86-87 0.7671761103960151 1104 88-89 1.0014262322775345 1104 90-91 0.9210035599772262 1104 92-93 1.0483885965264967 1104 94-95 1.181850752870183 1104 96-97 1.0329623788670297 1104 98-99 1.078185437193607 1104 100-101 1.2347698122305601 1105 1 0.13080380228569766 1105 2 0.1661291233211628 1105 3 0.16113053960005175 1105 4 0.2652473099800545 1105 5 0.3330081485714942 1105 6 0.3341460837300403 1105 7 0.2745744422612759 1105 8 0.33301219728409137 1105 9 0.23095040376023235 1105 10-11 0.33462674764693645 1105 12-13 0.33486133077328617 1105 14-15 0.412019180383389 1105 16-17 0.39825062930014354 1105 18-19 0.35403608072221715 1105 20-21 0.46016465093764936 1105 22-23 0.3936250614714183 1105 24-25 0.3947327234835356 1105 26-27 0.4377420515520356 1105 28-29 0.5211418073852627 1105 30-31 0.5603055354461475 1105 32-33 0.5717429640728398 1105 34-35 0.4926580298733967 1105 36-37 0.6544725719281601 1105 38-39 0.553573087198302 1105 40-41 0.5750885612091565 1105 42-43 0.5695405300282488 1105 44-45 0.6685409962350732 1105 46-47 0.8378357824726876 1105 48-49 0.8385964300587858 1105 50-51 0.9166176269147499 1105 52-53 0.9103571376368791 1105 54-55 0.7661378504325427 1105 56-57 0.6936822223316526 1105 58-59 0.6586068143110637 1105 60-61 0.6610840956126935 1105 62-63 0.7600449006671326 1105 64-65 0.8140523348633337 1105 66-67 0.7366063398024068 1105 68-69 0.7230560939013841 1105 70-71 0.578909748745172 1105 72-73 0.6315618421909299 1105 74-75 0.583776433956789 1105 76-77 0.5608271450131177 1105 78-79 0.6355782352448855 1105 80-81 0.6465274485671024 1105 82-83 0.6208198876686772 1105 84-85 0.583202081702904 1105 86-87 0.5283202390297177 1105 88-89 0.6485977889337313 1105 90-91 0.8360647485248691 1105 92-93 0.8875535815607485 1105 94-95 0.886774686943955 1105 96-97 0.7132470191952258 1105 98-99 0.6249608095699237 1105 100-101 1.0612100121342891 1106 1 0.11191179324719869 1106 2 0.10943699861851997 1106 3 0.222795030111417 1106 4 0.22412214825892107 1106 5 0.2071130929155771 1106 6 0.19007923700888085 1106 7 0.1935230909582657 1106 8 0.16184714931924304 1106 9 0.13996505256454128 1106 10-11 0.1681863415107756 1106 12-13 0.2822350392400139 1106 14-15 0.3985124104536837 1106 16-17 0.38444158245616933 1106 18-19 0.25330781395539503 1106 20-21 0.41400034823146115 1106 22-23 0.4060190111634867 1106 24-25 0.3018621659704408 1106 26-27 0.2254172618640311 1106 28-29 0.0836627491537314 1106 30-31 0.02071858760829315 1106 32-33 0.15160965220295708 1106 34-35 0.1610492250894353 1106 36-37 0.4016778519367392 1106 38-39 0.41503367493909593 1106 40-41 0.5484083010244518 1106 42-43 0.5049689345188781 1106 44-45 0.5330961960538261 1106 46-47 0.5643040910932413 1106 48-49 0.6895312825273834 1106 50-51 0.5480991415188399 1106 52-53 0.4524809459682757 1106 54-55 0.332202646201857 1106 56-57 0.5477242072237729 1106 58-59 0.7233742637974316 1106 60-61 0.7852459777187306 1106 62-63 0.7595968511581077 1106 64-65 0.7114087533290601 1106 66-67 0.6346486943428289 1106 68-69 0.7230352266587801 1106 70-71 0.7262858420396299 1106 72-73 0.8438836508442726 1106 74-75 0.5597379666855709 1106 76-77 0.6063177338867014 1106 78-79 0.6880998924616719 1106 80-81 0.567253390126897 1106 82-83 0.5089345024891898 1106 84-85 0.7925476253541923 1106 86-87 0.7458332214356496 1106 88-89 1.0531358179928603 1106 90-91 1.0078340522147933 1106 92-93 1.1112867908830388 1106 94-95 1.1674528127078432 1106 96-97 1.1790444161557652 1106 98-99 1.2166636958076786 1106 100-101 1.7341094260628758 1107 1 0.029457078340158205 1107 2 0.08839317125150714 1107 3 0.11414306815567699 1107 4 0.2532623289213518 1107 5 0.1824870832611296 1107 6 0.06955278286750399 1107 7 0.10742167146774761 1107 8 0.19691621207314824 1107 9 0.17213300171141555 1107 10-11 0.28599987207522304 1107 12-13 0.15160845605405626 1107 14-15 0.32224406939360506 1107 16-17 0.2874090106452414 1107 18-19 0.4661549200109292 1107 20-21 0.3437546855635887 1107 22-23 0.3666896845850758 1107 24-25 0.07814783246185897 1107 26-27 0.24798035033126808 1107 28-29 0.18187165801001015 1107 30-31 0.1304996897446955 1107 32-33 0.2629189768695923 1107 34-35 0.36558490352943096 1107 36-37 0.36556328901555446 1107 38-39 0.1085175204679345 1107 40-41 0.1996533423665099 1107 42-43 0.19512032294310444 1107 44-45 0.33644956316179986 1107 46-47 0.4972952969432711 1107 48-49 0.4278216806538495 1107 50-51 0.39071552062317494 1107 52-53 0.32239324343996145 1107 54-55 0.2633268874674499 1107 56-57 0.3017431098667984 1107 58-59 0.449399451390029 1107 60-61 0.4859156952544552 1107 62-63 0.4191598918310504 1107 64-65 0.32753813248118746 1107 66-67 0.2631621723335513 1107 68-69 0.32012037219240597 1107 70-71 0.4252212241550062 1107 72-73 0.3735806350243642 1107 74-75 0.3598162298409484 1107 76-77 0.19781990852704823 1107 78-79 0.2757570162010978 1107 80-81 0.07829267703604259 1107 82-83 0.033807847611782904 1107 84-85 0.2040875574862966 1107 86-87 0.16998606758581758 1107 88-89 0.1806336672496549 1107 90-91 0.46160083996033663 1107 92-93 0.47496432278573053 1107 94-95 0.320256545442156 1107 96-97 0.5357923729497251 1107 98-99 0.7272614829318975 1107 100-101 1.1525317364390162 1108 1 0.3099086546553238 1108 2 0.36686792582001715 1108 3 0.29983538918388675 1108 4 0.33004102773040955 1108 5 0.41961460185397925 1108 6 0.30031752124532574 1108 7 0.3112047397066675 1108 8 0.40697647905794554 1108 9 0.39561890758208307 1108 10-11 0.549216699522411 1108 12-13 0.48573614213680827 1108 14-15 0.6817194806988809 1108 16-17 0.7674378585790933 1108 18-19 0.7611439597060397 1108 20-21 0.7757783897940342 1108 22-23 0.7222841782806952 1108 24-25 0.7733092104630899 1108 26-27 0.7346320286095889 1108 28-29 0.746764449773039 1108 30-31 0.9243743338286308 1108 32-33 0.8836920603589036 1108 34-35 1.0693194878459096 1108 36-37 1.1281992276496027 1108 38-39 1.0176882176667448 1108 40-41 1.0548171739480594 1108 42-43 1.0909572068667117 1108 44-45 1.18851685150009 1108 46-47 1.0962252714671692 1108 48-49 1.0336341683492165 1108 50-51 1.0954276803909195 1108 52-53 1.1510187389309507 1108 54-55 1.173669279458494 1108 56-57 1.2764460723813684 1108 58-59 1.1417667597095758 1108 60-61 1.1187967201164213 1108 62-63 1.223687592312075 1108 64-65 1.1840944076230357 1108 66-67 1.238531421479209 1108 68-69 1.2257590891021266 1108 70-71 1.1856941187039425 1108 72-73 1.2221025365344182 1108 74-75 1.280169449908005 1108 76-77 1.355562350177383 1108 78-79 1.3724897875127198 1108 80-81 1.4240572391086879 1108 82-83 1.511894216577371 1108 84-85 1.613002428533754 1108 86-87 1.634036341005757 1108 88-89 1.6902325656361725 1108 90-91 1.8619812551002504 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72-73 -2.810582934294626 2316 74-75 -2.859870255063587 2316 76-77 -2.817689808623353 2316 78-79 -2.5960448646118124 2316 80-81 -2.5111202183983927 2316 82-83 -2.4798751365398815 2316 84-85 -2.3383770631755816 2316 86-87 -2.67616894469187 2316 88-89 -2.8508727495738704 2316 90-91 -3.0105262623584146 2316 92-93 -2.9022837675821407 2316 94-95 -3.050028297245774 2316 96-97 -2.971601806589849 2316 98-99 -3.1639049245852284 2316 100-101 -3.119548953537066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21188.0 3 6056.0 4 5701.0 5 6203.0 6 6843.0 7 8084.0 8 9816.0 9 11386.0 10 13324.0 11 15768.0 12 18521.0 13 21077.0 14 22222.0 15 23837.0 16 27012.0 17 31560.0 18 29431.0 19 24715.0 20 25957.0 21 27865.0 22 30836.0 23 34526.0 24 38800.0 25 44308.0 26 50510.0 27 57849.0 28 66840.0 29 78533.0 30 92125.0 31 109471.0 32 133360.0 33 165709.0 34 214765.0 35 292312.0 36 414961.0 37 575577.0 38 482184.0 39 71176.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56502906631579 18.70836634946944 13.229323417588091 23.497281166626685 2 15.538187825644936 20.399609074790163 39.8444166076648 24.217786491900103 3 19.76778815829775 24.598856192335955 29.248571148395968 26.384784500970326 4 12.642212255526236 15.78922525731413 37.031419298052924 34.537143189106715 5 13.553670212282789 37.75969140119963 34.07306815394817 14.613570232569407 6 32.32667013315303 35.43521857326443 17.609498495468245 14.628612798114297 7 29.05775760735873 32.41627772196192 20.659004652802125 17.866960017877226 8 26.582953878224508 34.63501298934114 19.97327335938974 18.80875977304461 9 27.01713455247746 16.29711064112613 20.321381388776366 36.36437341762004 10-11 25.212852950715885 26.37421164382639 27.34770126380659 21.065234141651132 12-13 25.99574201567396 23.7852742034745 27.840651611456053 22.378332169395488 14-15 22.855511433731984 25.155814120267895 26.217077959863094 25.771596486137028 16-17 22.25055516838631 28.522227410845506 26.822513185675177 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22.05978064853964 26.79212095788393 28.60633457957839 22.54176381399804 44-45 22.201300496112264 26.881261008481104 28.481204484948258 22.43623401045837 46-47 22.293254487215936 27.026417240006822 28.13127878546654 22.5490494873107 48-49 22.447406095312044 26.989469086673783 28.060683174803458 22.502441643210716 50-51 22.289917861938605 27.157119276475367 28.12706567784562 22.42589718374041 52-53 22.452885810453832 27.256519315468086 27.914728912574965 22.375865961503116 54-55 22.127282418243123 27.036736290705953 28.108308104759804 22.727673186291124 56-57 22.242565933408702 27.416754812360722 27.836276265003786 22.504402989226794 58-59 22.419052440286823 26.988537070746414 27.913043610057308 22.679366878909455 60-61 22.333803182458887 26.975506481751268 28.31796285782814 22.372727477961714 62-63 22.31318737466959 26.934322240123137 28.26976486930765 22.48272551589962 64-65 22.082879217739187 26.982777631335082 28.1959298098311 22.738413341094628 66-67 22.078644408372615 27.171714076921983 27.891809183593917 22.85783233111149 68-69 22.05875259566219 27.02205307968019 28.068888002390512 22.8503063222671 70-71 22.09089214405466 26.944063094255966 28.046295331439453 22.918749430249928 72-73 22.124716437387356 26.826960716880865 28.0151898711139 23.033132974617875 74-75 21.943443353038695 27.186968284726625 27.814937340347974 23.054651021886706 76-77 22.074475546054884 26.872128057093803 27.96808186550827 23.085314531343045 78-79 22.067726775818766 26.88951992188276 27.99980413080848 23.042949171489997 80-81 21.96857190204991 27.089347793357444 27.900665947698478 23.041414356894172 82-83 22.04882858078205 26.960602727936184 27.87689487818632 23.113673813095446 84-85 21.99613062822021 26.776544007166788 27.88940917552722 23.33791618908579 86-87 21.94172696340953 26.66625222132594 28.111129980167277 23.280890835097253 88-89 22.064840438840573 26.54867202959975 27.92766864048888 23.45881889107079 90-91 21.952461336160418 26.639123847137352 28.059171561735504 23.34924325496673 92-93 22.088861770898788 26.574583800276912 28.0144459413939 23.322108487430402 94-95 21.95179441473379 26.399221216451895 28.026090704985375 23.622893663828943 96-97 21.894882902139436 26.12307421150194 28.143545272515446 23.83849761384318 98-99 21.860948151932188 26.19180299572546 27.941842507364633 24.005406344977715 100-101 22.131954078693518 25.65243189465194 28.355568198756785 23.86004582789776 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3813.0 1 2518.0 2 1262.5 3 1338.5 4 1236.0 5 1282.0 6 1868.5 7 3033.5 8 4359.0 9 4880.0 10 4189.5 11 3492.0 12 3516.0 13 3694.5 14 3864.0 15 4057.0 16 4291.5 17 4444.0 18 4746.0 19 5181.0 20 5774.5 21 6678.0 22 7851.0 23 9288.0 24 10877.0 25 13402.0 26 17397.5 27 21892.5 28 25984.5 29 30250.0 30 35751.5 31 42036.5 32 49027.0 33 56076.5 34 63031.0 35 69923.0 36 78119.0 37 85795.0 38 89867.0 39 92548.0 40 94774.0 41 98500.0 42 103160.5 43 108394.5 44 113862.0 45 118089.0 46 120529.0 47 124099.0 48 137638.5 49 139157.0 50 127035.5 51 121082.0 52 116481.0 53 114219.5 54 110649.5 55 102895.0 56 93556.0 57 83773.0 58 75261.0 59 66788.0 60 57068.5 61 47140.0 62 38284.0 63 30323.5 64 23070.0 65 17266.0 66 12830.0 67 10169.0 68 7514.5 69 4988.5 70 3633.0 71 2731.5 72 2074.5 73 1684.0 74 1349.5 75 1026.0 76 771.5 77 508.5 78 318.5 79 243.0 80 182.5 81 133.0 82 107.0 83 88.5 84 67.5 85 50.0 86 37.0 87 25.5 88 16.5 89 14.5 90 11.5 91 9.0 92 9.0 93 5.5 94 3.5 95 2.0 96 2.0 97 2.0 98 1.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27259216297531447 2 0.16493275818320224 3 0.19296528558137288 4 0.16411715663174684 5 0.23471200202994166 6 0.1939923393869093 7 0.23906187697103706 8 0.2451939923393869 9 0.1479259554621138 10-11 0.20569773202353767 12-13 0.2114220466161598 14-15 0.17221275721656337 16-17 0.284388178006549 18-19 0.23036212708884618 20-21 0.19725474559273087 22-23 0.24300395113640486 24-25 0.23152511448629184 26-27 0.3409365522407897 28-29 0.31229987554524474 30-31 0.28765058421237055 32-33 0.19005026522154156 34-35 0.3584719855970807 36-37 0.39734899288312125 38-39 0.3797682483295272 40-41 0.3421901620328416 42-43 0.3745574606396733 44-45 0.3835894926354201 46-47 0.38899662884692066 48-49 0.40719662643032345 50-51 0.4001733908483464 52-53 0.3998864199320936 54-55 0.3953401964693515 56-57 0.3744668382450671 58-59 0.34874518190935344 60-61 0.31550186682132886 62-63 0.3823358828433683 64-65 0.41502035983132146 66-67 0.42082019308611546 68-69 0.4251700680272109 70-71 0.4240674955595026 72-73 0.41594168750981747 74-75 0.4114709827092471 76-77 0.3966089099938377 78-79 0.37217107091504453 80-81 0.38238119404067133 82-83 0.3916548857553679 84-85 0.3928631843501166 86-87 0.38846799821171807 88-89 0.39617090175324127 90-91 0.41550367926922105 92-93 0.41491463370428094 94-95 0.4234180350648252 96-97 0.4238258358405529 98-99 0.42118268266454006 100-101 0.39582351590725107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3310440.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.197908796428734 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22825393432423 38.745596289820085 2 13.77891190165103 14.384607735128139 3 5.000204087133049 7.830005907111033 4 2.3274227063250206 4.859463926219631 5 1.3018654196942914 3.3977326221213513 6 0.7660128122615115 2.399056014679336 7 0.5288731453883918 1.9324250545504567 8 0.34225646794100556 1.4292057518857955 9 0.25972868373449387 1.2201564730850634 >10 1.3048088469206218 12.242619227431579 >50 0.10177670696434138 3.663752449004689 >100 0.055755562310253685 5.217903586191521 >500 0.00308275272859114 1.0934814070747578 >1k 8.724771859792657E-4 0.8275191358212303 >5k 1.163302914639021E-4 0.4293347556372181 >10k+ 5.816514573195105E-5 0.3271396642381038 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10775 0.3254854339604403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8645 0.26114353379007016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5496 0.1660202269184761 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4151 0.1253911866700499 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.0207464868718356E-5 0.0 0.0 0.0 0.0 3 3.0207464868718356E-5 0.0 0.0 0.0 0.0 4 3.0207464868718356E-5 0.0 0.0 0.0 0.0 5 3.0207464868718356E-5 0.0 0.0 0.0 0.0 6 6.041492973743671E-5 0.0 0.0 0.0 0.0 7 6.041492973743671E-5 0.0 0.0 0.0 0.0 8 6.041492973743671E-5 0.0 0.0 0.0 0.0 9 6.041492973743671E-5 0.0 0.0 0.0 0.0 10-11 6.041492973743671E-5 0.0 0.0 0.0 0.0 12-13 7.551866217179589E-5 0.0 0.0 7.551866217179589E-5 1.8124478921231014E-4 14-15 9.062239460615507E-5 0.0 0.0 1.359335919092326E-4 1.9634852164666933E-4 16-17 9.062239460615507E-5 0.0 0.0 1.5103732434359178E-4 2.4165971894974685E-4 18-19 1.359335919092326E-4 0.0 0.0 1.8124478921231014E-4 2.7186718381846524E-4 20-21 1.5103732434359178E-4 0.0 0.0 3.624895784246203E-4 3.0207464868718355E-4 22-23 1.5103732434359178E-4 0.0 0.0 7.098754244148815E-4 3.0207464868718355E-4 24-25 2.7186718381846524E-4 0.0 0.0 0.0011176762001425793 3.322821135559019E-4 26-27 2.7186718381846524E-4 0.0 0.0 0.0018728628218605383 3.473858459902611E-4 28-29 3.9269704329333865E-4 0.0 0.0 0.00441028987083288 3.624895784246203E-4 30-31 5.135269027682121E-4 0.0 0.0 0.013245973344933 3.624895784246203E-4 32-33 5.135269027682121E-4 0.0 0.0 0.032880825509599934 3.624895784246203E-4 34-35 5.437343676369305E-4 0.0 0.0 0.0661543480624932 3.624895784246203E-4 36-37 6.041492973743671E-4 0.0 0.0 0.12303500441028986 3.624895784246203E-4 38-39 6.041492973743671E-4 0.0 0.0 0.20482171554234482 3.624895784246203E-4 40-41 6.343567622430855E-4 0.0 0.0 0.2918041106318193 3.624895784246203E-4 42-43 7.400828892835998E-4 0.0 0.0 0.38082550959993233 3.624895784246203E-4 44-45 8.45809016324114E-4 0.0 0.0 0.49215512137359385 3.624895784246203E-4 46-47 9.062239460615508E-4 0.0 0.0 0.6260195019393192 3.624895784246203E-4 48-49 9.666388757989874E-4 0.0 0.0 0.7691424704873069 3.624895784246203E-4 50-51 9.666388757989874E-4 0.0 0.0 0.9258587982262176 4.22904508162057E-4 52-53 9.666388757989874E-4 0.0 0.0 1.079312719759307 4.531119730307754E-4 54-55 9.666388757989874E-4 0.0 0.0 1.246767801259047 4.833194378994937E-4 56-57 9.666388757989874E-4 0.0 0.0 1.4553654499099817 4.833194378994937E-4 58-59 9.666388757989874E-4 0.0 0.0 1.6831750462174213 4.833194378994937E-4 60-61 9.968463406677059E-4 0.0 0.0 1.9326736023006004 4.833194378994937E-4 62-63 9.968463406677059E-4 0.0 0.0 2.1832445233866196 4.833194378994937E-4 64-65 9.968463406677059E-4 0.0 0.0 2.4384976015272892 4.833194378994937E-4 66-67 0.001087468735273861 0.0 0.0 2.730769927864574 4.833194378994937E-4 68-69 0.001087468735273861 0.0 0.0 3.0584151955631276 4.833194378994937E-4 70-71 0.0011629873974456568 0.0 0.0 3.4284717439373615 4.833194378994937E-4 72-73 0.0011931948623143751 0.0 0.0 3.7956132719517646 4.984231703338529E-4 74-75 0.0012082985947487342 0.0 0.0 4.160292891579367 5.286306352025713E-4 76-77 0.0012082985947487342 0.0 0.0 4.561251676514301 5.437343676369305E-4 78-79 0.0012385060596174526 0.0 0.0 5.012656927779993 5.437343676369305E-4 80-81 0.001359335919092326 0.0 0.0 5.509131716629813 5.437343676369305E-4 82-83 0.001359335919092326 0.0 0.0 6.009442853517961 5.739418325056488E-4 84-85 0.001359335919092326 0.0 0.0 6.509753990406109 5.739418325056488E-4 86-87 0.0013895433839610444 0.0 0.0 7.028612510723651 5.739418325056488E-4 88-89 0.0014197508488297628 0.0 0.0 7.590139075168255 5.739418325056488E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15230 0.0 28.674906 1 TACATGG 15645 0.0 27.352669 2 ACATGGG 15845 0.0 26.049284 3 TACCTGG 2540 0.0 24.094877 2 GTATAGG 930 0.0 24.016035 1 GTATCAA 19040 0.0 23.660711 1 GGTATCA 14315 0.0 23.00532 1 CATGGGA 9775 0.0 22.325943 4 CATGGGG 6345 0.0 21.160385 4 GAGTACT 10560 0.0 20.587202 12-13 ATCAACG 22130 0.0 20.066109 3 TATCAAC 22395 0.0 19.976957 2 TCAACGC 22345 0.0 19.873035 4 CAACGCA 22360 0.0 19.838486 5 AGTACAT 11400 0.0 19.601295 2 AACGCAG 22765 0.0 19.526937 6 GTACCTG 3520 0.0 19.305443 1 GTACTTT 11295 0.0 19.121744 14-15 AGTACTT 11045 0.0 18.716223 12-13 ATGGGAG 4390 0.0 18.69607 5 >>END_MODULE