FastQCFastQC Report
Thu 26 May 2016
SRR938754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310440
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108390.3274187117120383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92240.27863365594905815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62350.18834354345645896No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46150.1394074503691352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG24950.027.1986122
GTACATG154250.026.3665581
TACATGG156950.025.3979822
ACATGGG159500.023.7125853
GTATCAA203700.021.810641
GGTATCA158000.021.435591
CATGGGA96950.020.2644024
GTACCTG35550.020.2045821
GTATCGC1656.381109E-520.1293496
GAGTACT111350.019.79297812-13
CATGGGG64900.019.1574574
AGTACAT117900.018.7968182
ATCAACG235300.018.6552493
GTACTTT117850.018.56038514-15
TCAACGC236750.018.5409934
TATCAAC239550.018.4827562
CAACGCA238650.018.4132655
AGTACTT116350.018.26952612-13
AACGCAG242800.018.1739736
ATGGGAG45450.016.9146255
Produced by FastQC (version 0.11.5)