##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938754_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3310440 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.041033518203015 33.0 31.0 34.0 30.0 34.0 2 32.2384945807808 34.0 31.0 34.0 30.0 34.0 3 32.32488158673772 34.0 31.0 34.0 30.0 34.0 4 35.950049842317036 37.0 35.0 37.0 35.0 37.0 5 35.85601430625536 37.0 35.0 37.0 35.0 37.0 6 35.88029053539711 37.0 35.0 37.0 35.0 37.0 7 35.793088532038034 37.0 35.0 37.0 33.0 37.0 8 35.77838535058784 37.0 35.0 37.0 33.0 37.0 9 37.41571936056838 39.0 37.0 39.0 34.0 39.0 10-11 37.341268230205046 39.0 37.0 39.0 34.0 39.0 12-13 37.26270903565689 39.0 37.0 39.0 33.0 39.0 14-15 38.48275274585856 40.0 38.0 41.0 34.0 41.0 16-17 38.407965406411236 40.0 38.0 41.0 33.0 41.0 18-19 38.543112093860636 40.0 38.0 41.0 34.0 41.0 20-21 38.54328729715687 40.0 38.0 41.0 34.0 41.0 22-23 38.468637552712025 40.0 38.0 41.0 34.0 41.0 24-25 38.39369268133541 40.0 38.0 41.0 34.0 41.0 26-27 38.21971233431206 40.0 38.0 41.0 33.0 41.0 28-29 38.0533069622165 40.0 37.0 41.0 33.0 41.0 30-31 37.84117171735479 40.0 37.0 41.0 33.0 41.0 32-33 37.70981319703725 40.0 37.0 41.0 32.5 41.0 34-35 37.694191557617714 40.0 37.0 41.0 33.0 41.0 36-37 37.58933540556542 40.0 37.0 41.0 32.0 41.0 38-39 37.474716805016854 40.0 37.0 41.0 32.0 41.0 40-41 37.30775999565012 40.0 36.0 41.0 31.0 41.0 42-43 37.125794607363375 40.0 36.0 41.0 31.0 41.0 44-45 36.89723571488986 40.0 36.0 41.0 31.0 41.0 46-47 36.6127717161465 39.0 36.0 41.0 30.0 41.0 48-49 36.33010113459238 39.0 35.0 41.0 30.0 41.0 50-51 36.108544634550086 39.0 35.0 41.0 29.0 41.0 52-53 35.88883698239509 39.0 35.0 40.0 28.5 41.0 54-55 35.621540943197886 38.5 35.0 40.0 28.0 41.0 56-57 35.3547922632641 38.0 34.5 40.0 28.0 41.0 58-59 35.05220907190585 38.0 34.0 40.0 27.0 41.0 60-61 34.69304004905692 37.5 34.0 40.0 26.0 41.0 62-63 34.33039339181498 37.0 33.5 40.0 26.0 41.0 64-65 33.93998577228405 36.5 33.0 39.0 26.0 41.0 66-67 33.532823733401 36.0 33.0 39.0 25.0 40.0 68-69 33.19261850388468 35.5 32.0 39.0 24.5 40.0 70-71 32.760966820120586 35.0 32.0 38.0 23.0 40.0 72-73 32.27517535433357 35.0 31.5 37.5 22.0 39.5 74-75 31.78177613851935 35.0 31.0 37.0 20.5 39.0 76-77 29.838375110257246 33.0 28.5 35.0 18.5 37.5 78-79 30.79923288142966 34.0 30.5 36.0 19.0 38.0 80-81 30.854487620980898 34.0 31.0 35.5 19.0 37.0 82-83 30.53377904447747 34.0 30.5 35.0 18.0 37.0 84-85 30.134389839417118 34.0 30.0 35.0 14.5 36.5 86-87 29.5471645763101 34.0 29.5 35.0 7.0 36.0 88-89 28.94206359275504 34.0 29.0 35.0 2.0 35.5 90-91 28.417879043269174 33.5 28.5 35.0 2.0 35.0 92-93 28.113803905221058 33.0 28.0 35.0 2.0 35.0 94-95 27.75824407027465 33.0 27.0 35.0 2.0 35.0 96-97 27.13949701550247 33.0 26.5 35.0 2.0 35.0 98-99 26.284712304104588 33.0 25.0 35.0 2.0 35.0 100-101 22.586180688971858 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06506164772169853 1101 2 0.14461847788520998 1101 3 0.0492419555251189 1101 4 0.06993451487962687 1101 5 -0.0487636532679403 1101 6 0.058038600027842335 1101 7 0.17801612124767985 1101 8 0.17012725824785946 1101 9 0.2155895377871886 1101 10-11 0.2498963551118294 1101 12-13 0.1015400746654791 1101 14-15 0.2540048372683117 1101 16-17 0.26697438060958945 1101 18-19 0.2793002866621421 1101 20-21 0.2020364045124552 1101 22-23 0.1247612749364535 1101 24-25 0.16745777370881143 1101 26-27 0.10628112357865405 1101 28-29 0.15352501017698472 1101 30-31 0.1634158158181762 1101 32-33 0.2059804272003376 1101 34-35 0.2740698057003641 1101 36-37 0.3093531634401927 1101 38-39 0.2970777884746667 1101 40-41 0.4116021547213009 1101 42-43 0.44260411579376324 1101 44-45 0.3015370289455035 1101 46-47 0.4012907516779407 1101 48-49 0.3452758489121237 1101 50-51 0.29646200534963896 1101 52-53 0.23005907731534592 1101 54-55 0.24426307617711984 1101 56-57 0.23939903056080425 1101 58-59 0.23482725004897276 1101 60-61 0.20987539834846558 1101 62-63 0.07671037962647631 1101 64-65 0.10184905500015162 1101 66-67 0.11511399725239357 1101 68-69 0.08275472739648393 1101 70-71 0.16495687224492883 1101 72-73 0.06497831009794908 1101 74-75 -0.032074361092924164 1101 76-77 0.03707436676755904 1101 78-79 -0.027605752543912843 1101 80-81 0.09099849352264044 1101 82-83 0.26828182378615395 1101 84-85 0.28917317471416126 1101 86-87 0.3327686718631391 1101 88-89 0.3657248306661387 1101 90-91 0.14506318604127344 1101 92-93 0.07238424682242695 1101 94-95 0.13005052722122912 1101 96-97 0.03416644759414922 1101 98-99 -0.11479472664128565 1101 100-101 0.424895769926362 1102 1 0.24080671231487827 1102 2 0.12843002970928552 1102 3 0.16563300736581255 1102 4 0.0672612541328661 1102 5 -0.09932023645635724 1102 6 -0.02569191033033036 1102 7 0.14210607542879217 1102 8 0.11926801564404599 1102 9 0.1278577204277056 1102 10-11 0.2058645329526172 1102 12-13 0.21882673623705529 1102 14-15 0.3027463869878986 1102 16-17 0.34386168682080154 1102 18-19 0.3000956375772219 1102 20-21 0.29368345692911646 1102 22-23 0.24877894714495596 1102 24-25 0.2897371157038009 1102 26-27 0.2266894968789046 1102 28-29 0.2341440082164823 1102 30-31 0.24831279716202204 1102 32-33 0.23459111064388338 1102 34-35 0.29197798516567275 1102 36-37 0.16546690209161596 1102 38-39 0.18972322929224816 1102 40-41 0.38321576599548024 1102 42-43 0.3629537847975328 1102 44-45 0.33606161644629395 1102 46-47 0.3910108540227881 1102 48-49 0.3391400961495137 1102 50-51 0.31302183204274314 1102 52-53 0.18245030822260588 1102 54-55 0.20628527304695865 1102 56-57 0.32158504564669954 1102 58-59 0.37657869642684716 1102 60-61 0.4700399642135764 1102 62-63 0.33507209593335574 1102 64-65 0.22332185903486845 1102 66-67 0.41748667538670503 1102 68-69 0.4772198168005417 1102 70-71 0.38944325580180816 1102 72-73 0.5192049732940447 1102 74-75 0.3862156732066353 1102 76-77 0.25081551035679084 1102 78-79 0.17741701844656887 1102 80-81 0.48384399969028635 1102 82-83 0.4033545488100643 1102 84-85 0.4490121901803441 1102 86-87 0.5454275703637812 1102 88-89 0.4600823878289866 1102 90-91 0.5466923225712215 1102 92-93 0.6968901984781439 1102 94-95 0.8076214246585494 1102 96-97 0.8403841186950238 1102 98-99 0.932304994974821 1102 100-101 1.5130411018271595 1103 1 0.19351435292408325 1103 2 0.13452876194132557 1103 3 0.12025433289206688 1103 4 0.008733516465007085 1103 5 -0.004319331223229028 1103 6 0.005249951623824245 1103 7 0.06401536665332941 1103 8 0.1357515381931833 1103 9 0.16624567685286706 1103 10-11 0.14746540462588342 1103 12-13 0.17514978965464678 1103 14-15 0.2300274640828448 1103 16-17 0.11360128878317255 1103 18-19 0.0024605039339107293 1103 20-21 0.14717119874779883 1103 22-23 0.19260453963108404 1103 24-25 0.19774473070152254 1103 26-27 0.2169055274537044 1103 28-29 0.12419185309926917 1103 30-31 0.29039040655171533 1103 32-33 0.18001935215125542 1103 34-35 0.21574714409369733 1103 36-37 0.19454365261378825 1103 38-39 0.15531388287057268 1103 40-41 0.24505124760158736 1103 42-43 0.22800698380310536 1103 44-45 0.3204772553421691 1103 46-47 0.33737247920328883 1103 48-49 0.3293704779812643 1103 50-51 0.20613118641280437 1103 52-53 0.21220901943556925 1103 54-55 0.18886947076754268 1103 56-57 0.0725377483195686 1103 58-59 0.19270183683512698 1103 60-61 0.20682328452038945 1103 62-63 0.1655779556619592 1103 64-65 0.21796138857858693 1103 66-67 0.11287931473741253 1103 68-69 -0.008486883249354094 1103 70-71 0.15319369185923648 1103 72-73 0.15045190985265577 1103 74-75 0.2418555515962879 1103 76-77 0.18472410830522534 1103 78-79 0.009652756608986124 1103 80-81 0.17902302610101373 1103 82-83 0.25774641981654867 1103 84-85 0.25671887035926844 1103 86-87 0.18492289185401845 1103 88-89 0.18286578099602124 1103 90-91 0.15962015561973786 1103 92-93 0.12678182440932062 1103 94-95 0.17709122299027058 1103 96-97 0.18476791987933083 1103 98-99 0.21305063108876254 1103 100-101 0.6588625677749391 1104 1 0.15867152429157017 1104 2 0.12845708809479106 1104 3 0.10895976009979336 1104 4 0.10009065209460744 1104 5 0.05667243829751811 1104 6 0.04895351658605307 1104 7 0.12398128648420226 1104 8 0.08723196954521484 1104 9 0.14177114225277876 1104 10-11 0.19741798733434734 1104 12-13 0.044000342838437234 1104 14-15 0.06038551038279394 1104 16-17 0.2667193120903093 1104 18-19 0.23907897827044167 1104 20-21 0.18878296684763995 1104 22-23 0.1965373222564395 1104 24-25 0.11760627866888029 1104 26-27 0.10318799026799752 1104 28-29 0.11961993274739768 1104 30-31 -0.020170785974322314 1104 32-33 0.02410284748918201 1104 34-35 -0.0017911180544913918 1104 36-37 0.08680817543759645 1104 38-39 0.14251067593311006 1104 40-41 0.14430645704499767 1104 42-43 0.0840018534782061 1104 44-45 0.04065764557410034 1104 46-47 -0.01854811594023431 1104 48-49 0.049877837618545584 1104 50-51 -0.09364351417471539 1104 52-53 -0.1557908750730661 1104 54-55 -0.15532732014212058 1104 56-57 -0.18453447881841356 1104 58-59 -0.001491347992200076 1104 60-61 0.01514466934211356 1104 62-63 -0.045450520547582585 1104 64-65 -0.15747134130108975 1104 66-67 -0.09476590273627039 1104 68-69 -0.09058849726721974 1104 70-71 -0.2019540175787995 1104 72-73 -0.07587873452734328 1104 74-75 0.10635566246986272 1104 76-77 -0.039909524494408544 1104 78-79 -0.051260432610227724 1104 80-81 0.022660336028181405 1104 82-83 0.03450059049296428 1104 84-85 0.0892747431065466 1104 86-87 0.27035851736398087 1104 88-89 0.3401542116074623 1104 90-91 0.34713240015302205 1104 92-93 0.4949431852909676 1104 94-95 0.5372616130715002 1104 96-97 0.4987925661065091 1104 98-99 0.7217999556211012 1104 100-101 1.4628434673794537 1105 1 0.11515259280132994 1105 2 0.11695415056626501 1105 3 0.0722458517781277 1105 4 0.06267602028113828 1105 5 -0.0903960631418741 1105 6 -0.03877883199165666 1105 7 0.023274896904808884 1105 8 0.039282313513119504 1105 9 0.06748067677069258 1105 10-11 0.03291496039599906 1105 12-13 0.07984926311086582 1105 14-15 0.1505215276742362 1105 16-17 0.15702073755317514 1105 18-19 0.136025190984725 1105 20-21 0.0597449697524155 1105 22-23 0.059735875480747325 1105 24-25 0.0800668889636924 1105 26-27 0.11594058601188806 1105 28-29 0.0766027619028975 1105 30-31 0.05544523210725316 1105 32-33 0.22462711653788858 1105 34-35 0.20984427076906798 1105 36-37 0.17860100352226027 1105 38-39 0.26146446664967016 1105 40-41 0.185789755602066 1105 42-43 0.30569233850570043 1105 44-45 0.21280300307304145 1105 46-47 0.1621287320100393 1105 48-49 0.14202128736139485 1105 50-51 0.09052590496293789 1105 52-53 0.20782782634282881 1105 54-55 0.17588071052337284 1105 56-57 0.19037624131532738 1105 58-59 0.21043007428934857 1105 60-61 0.18930447692385854 1105 62-63 0.17655406846353117 1105 64-65 0.19040837233796992 1105 66-67 0.17866414915262396 1105 68-69 0.18337008623168316 1105 70-71 0.13285314830657313 1105 72-73 0.16586515207604435 1105 74-75 0.230238689470589 1105 76-77 0.24541489280826667 1105 78-79 -0.0034082436810329853 1105 80-81 0.12048260905459074 1105 82-83 0.14682414904548224 1105 84-85 0.1262214028699411 1105 86-87 0.1622217556837846 1105 88-89 0.03221625682596141 1105 90-91 0.16984324878739088 1105 92-93 0.17415074915401263 1105 94-95 0.47092953147567584 1105 96-97 0.5519664588542312 1105 98-99 0.5754069661828964 1105 100-101 1.2527119309756216 1106 1 0.1739862928019278 1106 2 0.07334519076849944 1106 3 0.0909470745985459 1106 4 0.03289190681103804 1106 5 -0.016191552240229612 1106 6 0.05655167532082572 1106 7 0.07696452350040772 1106 8 0.09996485120830556 1106 9 0.12506055359433788 1106 10-11 0.09276787614599868 1106 12-13 0.15390561659003055 1106 14-15 0.11290116839993658 1106 16-17 0.19666044970447416 1106 18-19 0.018715515142439187 1106 20-21 0.009937544591295477 1106 22-23 0.12465863687277334 1106 24-25 0.17614772269946144 1106 26-27 0.15326701526895192 1106 28-29 0.16128590772915885 1106 30-31 0.14702380919978708 1106 32-33 0.15474301916537314 1106 34-35 0.13828960157448478 1106 36-37 0.19405677391104348 1106 38-39 0.2600741120854053 1106 40-41 0.30267463400566896 1106 42-43 0.22110011423649922 1106 44-45 0.2670608148743625 1106 46-47 0.3092058275283591 1106 48-49 0.2295131640419541 1106 50-51 0.2801739640112757 1106 52-53 0.27435647110485917 1106 54-55 0.18939374062586012 1106 56-57 0.16825249260462982 1106 58-59 0.2874248336304248 1106 60-61 0.2718767521091294 1106 62-63 0.38932601335016415 1106 64-65 0.331218140592334 1106 66-67 0.32774688478851033 1106 68-69 0.2446804298257348 1106 70-71 0.25562676257261785 1106 72-73 0.19787580033184327 1106 74-75 0.3303337844761671 1106 76-77 0.3476774981133133 1106 78-79 0.34534270279747403 1106 80-81 0.3725577072796824 1106 82-83 0.5431014329651873 1106 84-85 0.5336820383072869 1106 86-87 0.612504953976245 1106 88-89 0.6026497588858568 1106 90-91 0.5619044553909802 1106 92-93 0.6064866282909485 1106 94-95 0.8370405332821846 1106 96-97 1.0614509187205599 1106 98-99 1.2584404074296884 1106 100-101 1.71885870519802 1107 1 0.018021564990426953 1107 2 -0.004656290854597955 1107 3 -0.028206488466025803 1107 4 -0.050860423160763446 1107 5 -0.12187190400623393 1107 6 -0.093516431547215 1107 7 0.005372256617071969 1107 8 0.014358102654078664 1107 9 -0.04156469135141805 1107 10-11 -0.0984654555941944 1107 12-13 -0.001682731161245954 1107 14-15 0.023044312308556414 1107 16-17 -0.05169243946055957 1107 18-19 -0.029849461199390248 1107 20-21 -0.03602801962033908 1107 22-23 -0.004135632591449223 1107 24-25 -0.02811732055436522 1107 26-27 0.0169857697496667 1107 28-29 0.04046014182560498 1107 30-31 0.027308663188222226 1107 32-33 -0.032339378196382995 1107 34-35 0.07457861366644636 1107 36-37 -0.04139703290442043 1107 38-39 -0.08359732604488102 1107 40-41 -0.26910343319310925 1107 42-43 -0.3318511833785678 1107 44-45 -0.10060574823800295 1107 46-47 -0.12357807057420445 1107 48-49 -0.17284906890743912 1107 50-51 -0.24085373278172995 1107 52-53 -0.10282593581575128 1107 54-55 0.024138997042243204 1107 56-57 -0.10591050983287431 1107 58-59 -0.04384159565792345 1107 60-61 -0.06603009681310112 1107 62-63 -0.03738263263750241 1107 64-65 -0.041581529627038094 1107 66-67 2.818941083972959E-4 1107 68-69 -0.09159902340755366 1107 70-71 -0.05058171613502793 1107 72-73 -0.12681532904731085 1107 74-75 -0.09274906693137552 1107 76-77 -0.12278204401735593 1107 78-79 -0.07489997272707072 1107 80-81 0.17372687518103547 1107 82-83 0.28902816431346423 1107 84-85 0.1247313620180357 1107 86-87 0.15827814615709812 1107 88-89 0.2365233668770621 1107 90-91 0.39532043302325093 1107 92-93 0.4623790672337371 1107 94-95 0.5561869272027522 1107 96-97 0.7091585849252162 1107 98-99 0.9090414206687036 1107 100-101 1.605058700418592 1108 1 0.09956270727163741 1108 2 0.10929157864431716 1108 3 0.1123461988655663 1108 4 0.08129426112074611 1108 5 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-1.9054242469111067 2316 92-93 -1.7368266250561817 2316 94-95 -1.5916550145549877 2316 96-97 -1.7947493630508333 2316 98-99 -1.8115432711252524 2316 100-101 -1.7441410397297368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 29.0 5 86.0 6 211.0 7 502.0 8 1195.0 9 2082.0 10 3422.0 11 5236.0 12 7531.0 13 10156.0 14 12425.0 15 15887.0 16 19828.0 17 26376.0 18 25202.0 19 18849.0 20 18313.0 21 19153.0 22 21064.0 23 23805.0 24 27712.0 25 33440.0 26 40188.0 27 49312.0 28 60532.0 29 73736.0 30 89803.0 31 108474.0 32 132491.0 33 167989.0 34 221284.0 35 307764.0 36 455860.0 37 691715.0 38 566638.0 39 52131.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16007665129866 19.624773181371033 13.856275484452496 23.358874682877808 2 16.073029567066612 21.061490315486765 38.881508198305966 23.98397191914066 3 19.7928674133952 24.801778615531468 29.596337646959313 25.809016324114015 4 13.073035608559588 16.515327267674387 36.57534950036853 33.836287623397496 5 13.451833400110106 38.009097353282975 34.12670786884196 14.412361377764965 6 32.32721224395101 36.018806731949695 17.177908735874407 14.47607228822489 7 28.864320150795663 32.635601309795675 20.590525730718575 17.909552808690083 8 26.573718297265618 35.22622370440183 19.599630260630008 18.60042773770254 9 26.671530068510528 16.37543649786735 20.287424028225857 36.66560940539626 10-11 25.1889325890214 26.690817534829208 27.152191249501577 20.968058626647817 12-13 25.95860369014391 23.892035499812714 27.81657423182417 22.33278657821921 14-15 22.65387682604125 25.420956126678025 26.14069428837254 25.784472758908183 16-17 22.171014165716237 28.683963017220375 26.81361782880156 22.33140498826182 18-19 21.965977332318364 27.34699919043994 29.06790336027839 21.619120116963302 20-21 22.79292178683196 26.430263046604075 28.97922934715627 21.79758581940769 22-23 22.428665071712523 26.49108577711724 28.752099418808374 22.32814973236186 24-25 22.3464726696257 26.624468705486315 29.051752665855794 21.977305959032194 26-27 22.2630598702845 26.678495051275547 29.05627524903724 22.002169829402714 28-29 21.926768042918766 26.939409866966322 29.11474003455734 22.019082055557572 30-31 22.072307608656253 26.680667826633318 29.03215282560626 22.214871739104165 32-33 21.930332523773274 26.862894358453858 28.969759306919922 22.237013810852936 34-35 22.469387622550332 26.81663447597087 28.79618478703011 21.917793114448685 36-37 22.448172181532865 26.58101357683702 28.83198656636964 22.138827675260476 38-39 22.20802118664934 26.7894588806254 29.02265522540875 21.97986470731651 40-41 22.255053572566837 26.710698471957596 29.010782787537725 22.023465167937843 42-43 22.060316688291778 26.905514629802774 28.6383732680401 22.395795413865347 44-45 22.213713500984344 26.91193231850973 28.507362863328687 22.366991317177234 46-47 22.303475673127622 27.144291357284047 28.11523963625802 22.436993333330314 48-49 22.421282593631076 27.160745000734053 28.0136968921449 22.404275513489967 50-51 22.300508271110953 27.26542530021707 28.037453050019362 22.396613378652614 52-53 22.49622406689141 27.31090126992182 27.859227172218798 22.333647490967966 54-55 22.126726356617247 27.087517067217654 28.013813873684462 22.771942702480636 56-57 22.2589617394312 27.483478167619097 27.748250396289865 22.509309696659837 58-59 22.429392448741908 27.081010811742345 27.851515294772263 22.63808144474348 60-61 22.342848615512697 27.108676128480326 28.171929388927303 22.376545867079674 62-63 22.27016689458385 27.074627848549408 28.13312115436956 22.522084102497182 64-65 22.028587857357657 27.128377413970522 28.02074920745779 22.82228552121403 66-67 22.029784998566576 27.306761982528847 27.76119799761771 22.902255021286866 68-69 22.044330139190212 27.12503859010631 27.937122443764174 22.893508826939307 70-71 22.035175723805605 27.077607439381485 27.86484501364095 23.02237182317196 72-73 22.07978395621126 26.957156752576694 27.803569918198185 23.15948937301386 74-75 21.906502294948062 27.305041182797307 27.584416314106036 23.204040208148594 76-77 22.02878229719159 26.996952085723645 27.79607748787061 23.178188129214156 78-79 22.0163339621754 27.02200352318939 27.81129535062722 23.15036716400799 80-81 21.871789012269023 27.227771299057927 27.66172050263857 23.23871918603448 82-83 21.924649235397055 27.117176688936812 27.641190172458945 23.316983903207184 84-85 21.843531373473013 26.950737666292095 27.601738741677845 23.60399221855705 86-87 21.809515357038695 26.89309333545812 27.743213905406456 23.554177402096734 88-89 21.851983828838996 26.81914572581749 27.586189126435677 23.742681318907838 90-91 21.738135646031935 26.825123395831447 27.712020747087408 23.724720211049213 92-93 21.768015482007062 26.80305102957371 27.66869556115705 23.760237927262175 94-95 21.612868779453308 26.646051019648354 27.68485851009408 24.056221690804264 96-97 21.592989804513635 26.37443019203969 27.753855950372692 24.278724053073983 98-99 21.47358055122582 26.475498725244982 27.555068208455673 24.495852515073523 100-101 21.63259233750502 25.933117045924824 28.086037681133153 24.348252935437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1544.0 1 1056.0 2 724.0 3 912.5 4 827.5 5 790.0 6 1363.0 7 2619.5 8 3849.5 9 4296.0 10 3707.0 11 2970.0 12 2921.5 13 3120.5 14 3276.0 15 3490.0 16 3759.0 17 3957.5 18 4288.0 19 4703.5 20 5343.5 21 6375.0 22 7459.5 23 8924.0 24 10819.0 25 13314.0 26 17177.5 27 21658.5 28 25729.5 29 30136.0 30 35804.0 31 42692.0 32 49914.5 33 56574.0 34 63574.5 35 70207.5 36 78357.0 37 86521.0 38 90680.0 39 92907.0 40 94946.0 41 98873.0 42 103557.0 43 108983.5 44 114498.0 45 118848.5 46 120912.5 47 124897.0 48 139746.5 49 140983.5 50 128410.5 51 122442.0 52 117834.0 53 115786.5 54 112447.0 55 104333.5 56 94720.5 57 84749.0 58 75866.0 59 67186.5 60 56843.0 61 46194.5 62 37475.5 63 29585.5 64 21931.5 65 16386.5 66 12025.5 67 9353.5 68 6814.5 69 4223.5 70 2941.0 71 2182.5 72 1691.0 73 1418.5 74 1186.5 75 932.5 76 635.0 77 378.5 78 222.5 79 141.5 80 95.5 81 65.0 82 40.0 83 33.0 84 24.5 85 11.0 86 5.5 87 3.0 88 3.0 89 3.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2323256123053129 2 0.0 3 0.0 4 0.0 5 0.027367963171058832 6 0.004108215222145697 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002673360640881575 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.473858459902611E-4 26-27 0.0012536097920518119 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00660033107381496 36-37 0.016251616099370477 38-39 0.013019417358417612 40-41 0.007763318471260618 42-43 0.007400828892835997 44-45 0.0033530286004277376 46-47 4.984231703338529E-4 48-49 0.0016312031029107911 50-51 6.041492973743671E-5 52-53 0.0 54-55 0.0 56-57 0.001434854581264122 58-59 3.473858459902611E-4 60-61 0.002446804654366187 62-63 8.156015514553956E-4 64-65 0.0014650620461328404 66-67 0.0055279660709754595 68-69 1.8124478921231014E-4 70-71 1.0572612704051425E-4 72-73 0.0 74-75 4.984231703338529E-4 76-77 0.05884414156426336 78-79 0.13981525114486293 80-81 8.911202136271915E-4 82-83 8.760164811928324E-4 84-85 0.0 86-87 0.10386836795108809 88-89 0.3505425260690422 90-91 0.38727480334940373 92-93 4.984231703338529E-4 94-95 2.8697091625282437E-4 96-97 1.3593359190923262E-4 98-99 0.0 100-101 9.062239460615507E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3310440.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.10815985769133 #Duplication Level Percentage of deduplicated Percentage of total 1 71.62587033656403 35.17414690434258 2 14.859805907231305 14.594754478931618 3 5.576407160093205 8.215412827482949 4 2.649989701702838 5.205444715698349 5 1.463623462089116 3.593792747386996 6 0.8792330548679202 2.5906510446372066 7 0.5840988149207075 2.007881258306991 8 0.4037677395413556 1.5862632559020453 9 0.29702087955747725 1.3127533950942607 >10 1.4743982888029394 13.09892998869087 >50 0.11641705233649416 3.992482703418345 >100 0.06456492294679732 5.722107875151182 >500 0.003632795916439472 1.224156078698618 >1k 9.851649931688353E-4 0.8860428687890584 >5k 1.2314562414610442E-4 0.46743803394226024 >10k+ 6.157281207305221E-5 0.32774182352675846 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10839 0.3274187117120383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9224 0.27863365594905815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6235 0.18834354345645896 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4615 0.1394074503691352 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.0207464868718356E-5 0.0 4 0.0 0.0 0.0 3.0207464868718356E-5 0.0 5 0.0 0.0 0.0 3.0207464868718356E-5 0.0 6 6.041492973743671E-5 0.0 0.0 3.0207464868718356E-5 3.0207464868718356E-5 7 9.062239460615507E-5 0.0 0.0 3.0207464868718356E-5 3.0207464868718356E-5 8 9.062239460615507E-5 0.0 0.0 6.041492973743671E-5 3.0207464868718356E-5 9 9.062239460615507E-5 0.0 0.0 6.041492973743671E-5 3.0207464868718356E-5 10-11 9.062239460615507E-5 0.0 0.0 6.041492973743671E-5 3.0207464868718356E-5 12-13 1.2082985947487342E-4 0.0 0.0 9.062239460615507E-5 9.062239460615507E-5 14-15 1.8124478921231014E-4 0.0 0.0 1.2082985947487342E-4 1.2082985947487342E-4 16-17 1.8124478921231014E-4 0.0 0.0 1.2082985947487342E-4 1.5103732434359178E-4 18-19 1.9634852164666933E-4 0.0 0.0 1.5103732434359178E-4 1.5103732434359178E-4 20-21 2.114522540810285E-4 0.0 0.0 3.0207464868718355E-4 1.8124478921231014E-4 22-23 2.4165971894974685E-4 0.0 0.0 6.192530298087263E-4 1.8124478921231014E-4 24-25 3.322821135559019E-4 0.0 0.0 0.0010421575379707835 1.8124478921231014E-4 26-27 3.322821135559019E-4 0.0 0.0 0.0019181740191636157 1.8124478921231014E-4 28-29 4.22904508162057E-4 0.0 0.0 0.004531119730307754 1.8124478921231014E-4 30-31 4.833194378994937E-4 0.0 0.0 0.01362356665579198 1.8124478921231014E-4 32-33 4.984231703338529E-4 0.0 0.0 0.0338172569205302 2.114522540810285E-4 34-35 5.286306352025713E-4 0.0 0.0 0.067785551165404 2.4165971894974685E-4 36-37 5.739418325056488E-4 0.0 0.0 0.1254969127970904 2.4165971894974685E-4 38-39 6.041492973743671E-4 0.0 0.0 0.20868827104554077 2.4165971894974685E-4 40-41 6.192530298087263E-4 0.0 0.0 0.2971810393784512 2.4165971894974685E-4 42-43 6.343567622430855E-4 0.0 0.0 0.38741073694131295 2.4165971894974685E-4 44-45 6.947716919805222E-4 0.0 0.0 0.5003262406205822 2.4165971894974685E-4 46-47 8.609127487584732E-4 0.0 0.0 0.6362296250649461 2.4165971894974685E-4 48-49 8.760164811928324E-4 0.0 0.0 0.7818900206619059 3.0207464868718355E-4 50-51 8.760164811928324E-4 0.0 0.0 0.9412192941119609 3.322821135559019E-4 52-53 8.760164811928324E-4 0.0 0.0 1.0968179456507292 3.322821135559019E-4 54-55 9.062239460615508E-4 3.0207464868718356E-5 0.0 1.2665990019453606 3.624895784246203E-4 56-57 9.666388757989874E-4 3.0207464868718356E-5 0.0 1.4774017955317118 3.7759331085897947E-4 58-59 9.666388757989874E-4 3.0207464868718356E-5 0.0 1.7087003540314882 4.22904508162057E-4 60-61 9.666388757989874E-4 3.0207464868718356E-5 0.0 1.9618540133637825 4.6821570546513457E-4 62-63 9.968463406677059E-4 3.0207464868718356E-5 0.0 2.215551407063714 5.135269027682121E-4 64-65 9.968463406677059E-4 3.0207464868718356E-5 0.0 2.474686144440014 5.135269027682121E-4 66-67 0.0010270538055364242 3.0207464868718356E-5 0.0 2.772350503256365 5.135269027682121E-4 68-69 0.0010270538055364242 3.0207464868718356E-5 0.0 3.1044815794879232 5.135269027682121E-4 70-71 0.0011025724677082203 3.0207464868718356E-5 0.0 3.4800207827358296 5.286306352025713E-4 72-73 0.0011478836650112977 3.0207464868718356E-5 0.0 3.8531584925266733 5.437343676369305E-4 74-75 0.0011478836650112977 3.0207464868718356E-5 0.0 4.224544169355131 5.437343676369305E-4 76-77 0.0011478836650112977 3.0207464868718356E-5 0.0 4.633085027972113 5.739418325056488E-4 78-79 0.0012082985947487342 3.0207464868718356E-5 0.0 5.090546875943984 6.041492973743671E-4 80-81 0.0014046471163954035 3.0207464868718356E-5 0.0 5.592232452483658 6.041492973743671E-4 82-83 0.0014801657785671995 3.0207464868718356E-5 0.0 6.09903819431858 6.343567622430855E-4 84-85 0.0015103732434359179 3.0207464868718356E-5 0.0 6.606432981718442 6.645642271118038E-4 86-87 0.0015405807083046362 3.0207464868718356E-5 0.0 7.131635069658413 6.645642271118038E-4 88-89 0.0015405807083046362 3.0207464868718356E-5 0.0 7.703220719904303 6.645642271118038E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2495 0.0 27.198612 2 GTACATG 15425 0.0 26.366558 1 TACATGG 15695 0.0 25.397982 2 ACATGGG 15950 0.0 23.712585 3 GTATCAA 20370 0.0 21.81064 1 GGTATCA 15800 0.0 21.43559 1 CATGGGA 9695 0.0 20.264402 4 GTACCTG 3555 0.0 20.204582 1 GTATCGC 165 6.381109E-5 20.129349 6 GAGTACT 11135 0.0 19.792978 12-13 CATGGGG 6490 0.0 19.157457 4 AGTACAT 11790 0.0 18.796818 2 ATCAACG 23530 0.0 18.655249 3 GTACTTT 11785 0.0 18.560385 14-15 TCAACGC 23675 0.0 18.540993 4 TATCAAC 23955 0.0 18.482756 2 CAACGCA 23865 0.0 18.413265 5 AGTACTT 11635 0.0 18.269526 12-13 AACGCAG 24280 0.0 18.173973 6 ATGGGAG 4545 0.0 16.914625 5 >>END_MODULE