FastQCFastQC Report
Thu 26 May 2016
SRR938753_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938753_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848614
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67730.7981249425533872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54760.6452874923109918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.41538320131414286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26140.3080316846057218No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19270.22707615005173143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.14812388200053264No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11670.13751835345634175No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.12019599016749666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.040.952781
GTATCAA63700.035.9330561
TATCAAC78600.029.1661172
ATCAACG78950.029.0368143
TCAACGC79850.028.7095414
CAACGCA80650.028.4247595
AACGCAG82250.027.8520956
GTACATG51600.026.3583741
TACATGG52400.025.6710012
ACATGGG52150.024.8858263
ACGCAGA91600.024.7522167
CGCAGAG92150.024.3476498
CATGGGG12850.023.9588534
TAAGGCG1201.7436694E-423.6824045
GAGTACT52150.023.37343612-13
GCAGAGT95600.022.8748429
GTGCTAA1503.0867704E-522.115321
AGTACTT54000.021.87139312-13
CATGGGA37000.021.6341974
GTACTTT59700.020.4967914-15
Produced by FastQC (version 0.11.5)