##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938753_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 848614 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31017871493989 31.0 31.0 34.0 28.0 34.0 2 31.34834565538631 33.0 31.0 34.0 28.0 34.0 3 31.327648377236294 33.0 31.0 34.0 28.0 34.0 4 34.91603603051564 37.0 35.0 37.0 32.0 37.0 5 34.964987615099446 37.0 35.0 37.0 32.0 37.0 6 35.05659345709592 37.0 35.0 37.0 32.0 37.0 7 34.969261643102755 37.0 35.0 37.0 32.0 37.0 8 34.956287546517025 37.0 35.0 37.0 32.0 37.0 9 36.46023751670371 39.0 37.0 39.0 32.0 39.0 10-11 36.3536113003085 39.0 37.0 39.0 32.0 39.0 12-13 36.2370264926103 39.0 37.0 39.0 32.0 39.0 14-15 37.29649758311788 40.0 37.0 41.0 32.0 41.0 16-17 37.225045780531545 40.0 37.0 41.0 31.0 41.0 18-19 37.20371040308079 40.0 37.0 41.0 31.0 41.0 20-21 37.121798603369726 40.0 36.5 41.0 31.0 41.0 22-23 36.979583768356406 40.0 36.0 41.0 31.0 41.0 24-25 36.939503119203785 40.0 36.0 41.0 30.5 41.0 26-27 36.81625332601159 39.5 36.0 41.0 30.0 41.0 28-29 36.657433179278215 39.0 36.0 41.0 30.0 41.0 30-31 36.380189344036275 39.0 35.5 41.0 30.0 41.0 32-33 36.074004788985334 39.0 35.0 41.0 29.5 41.0 34-35 35.67862538209363 38.5 35.0 41.0 28.0 41.0 36-37 35.38248426257404 38.0 35.0 40.0 26.5 41.0 38-39 35.16211552013047 38.0 35.0 40.0 25.5 41.0 40-41 34.91468029045008 38.0 34.5 40.0 24.5 41.0 42-43 34.564493397469285 38.0 34.0 40.0 24.0 41.0 44-45 34.3472403236336 38.0 34.0 40.0 22.5 41.0 46-47 33.988762264115365 38.0 33.5 40.0 18.5 41.0 48-49 33.632114247467044 38.0 33.0 40.0 13.5 41.0 50-51 32.44321446499822 37.0 31.5 39.5 8.5 40.5 52-53 32.523746956802505 37.5 32.5 39.5 8.0 40.5 54-55 33.01529022618057 38.0 33.0 40.0 7.0 41.0 56-57 32.92468601743549 38.0 33.0 40.0 2.0 41.0 58-59 32.69075869594421 37.5 32.5 40.0 2.0 41.0 60-61 32.40281270400912 37.0 32.0 40.0 2.0 41.0 62-63 32.01424322483485 36.5 31.5 40.0 2.0 41.0 64-65 31.598398683029032 36.0 31.0 39.5 2.0 41.0 66-67 31.17020812760572 35.5 31.0 39.0 2.0 41.0 68-69 30.71397066275126 35.0 30.0 39.0 2.0 40.5 70-71 30.261325526093138 35.0 29.5 38.0 2.0 40.0 72-73 29.801508106158984 35.0 29.0 37.5 2.0 40.0 74-75 29.28332610586203 35.0 29.0 37.0 2.0 39.0 76-77 28.793212815249337 34.5 28.0 36.5 2.0 39.0 78-79 28.30481997704492 34.0 27.0 36.0 2.0 38.5 80-81 27.80133075815388 34.0 26.0 35.5 2.0 37.0 82-83 27.281983328109128 34.0 25.5 35.0 2.0 37.0 84-85 26.802033669017952 34.0 25.0 35.0 2.0 36.5 86-87 26.327001439995097 33.5 23.5 35.0 2.0 36.0 88-89 25.88644896266147 33.0 21.0 35.0 2.0 36.0 90-91 25.383799348113513 33.0 18.5 35.0 2.0 35.0 92-93 24.853435130695466 33.0 10.5 35.0 2.0 35.0 94-95 24.253650069407293 32.0 2.0 35.0 2.0 35.0 96-97 23.593188422533686 32.0 2.0 35.0 2.0 35.0 98-99 22.837016594116996 31.5 2.0 35.0 2.0 35.0 100-101 20.853085737449533 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38689972902933434 1101 2 0.20582613303434272 1101 3 0.4694537446042588 1101 4 0.37033806861780505 1101 5 0.23115249178362518 1101 6 0.0026500653179226674 1101 7 0.37715512634486004 1101 8 0.5297459101175477 1101 9 0.5366652661111786 1101 10-11 0.5280923103432968 1101 12-13 0.4499405097270781 1101 14-15 0.49584837991248065 1101 16-17 0.5703320969174968 1101 18-19 0.6390403393617845 1101 20-21 0.6113754360778572 1101 22-23 0.7158644427727481 1101 24-25 0.74828157887179 1101 26-27 0.6507612305003292 1101 28-29 0.525715260430097 1101 30-31 0.6141407594559567 1101 32-33 0.4397873294814687 1101 34-35 0.6346954031978882 1101 36-37 0.718851496230414 1101 38-39 0.6547813581260868 1101 40-41 0.7152384878685822 1101 42-43 0.606314742422704 1101 44-45 0.6980343786526433 1101 46-47 0.8881754900841798 1101 48-49 0.9045130756507049 1101 50-51 0.6446954851907023 1101 52-53 0.750201658741311 1101 54-55 0.643265824613124 1101 56-57 0.6396384386962595 1101 58-59 0.46575814703487595 1101 60-61 0.5287079803891004 1101 62-63 0.672661841306045 1101 64-65 0.6271031849406832 1101 66-67 0.4858927753623483 1101 68-69 0.37240309881392264 1101 70-71 0.3299461940890822 1101 72-73 -0.056144808469763774 1101 74-75 -0.0887620240291227 1101 76-77 -0.2624988702336424 1101 78-79 -0.5966452210729862 1101 80-81 -0.3565736144803928 1101 82-83 -0.5380617425154064 1101 84-85 -0.5428881524157951 1101 86-87 -0.9101747361698038 1101 88-89 -0.8638653244447632 1101 90-91 -0.9416669290804762 1101 92-93 -1.1996675060117106 1101 94-95 -1.3671621356898775 1101 96-97 -0.8629288613880135 1101 98-99 -0.8060258605346107 1101 100-101 -0.7116261177923064 1102 1 0.4339879654291998 1102 2 0.4561613586693909 1102 3 0.5139197019710764 1102 4 0.47862848053805607 1102 5 0.4847240546243299 1102 6 0.5314199438847851 1102 7 0.39992853887916624 1102 8 0.5635003119121009 1102 9 0.6185355118597684 1102 10-11 0.5675157849100074 1102 12-13 0.8864967085816673 1102 14-15 0.6752724184101595 1102 16-17 0.8814951105646216 1102 18-19 0.8004091878147364 1102 20-21 0.7926385161435263 1102 22-23 0.772116557513904 1102 24-25 0.7465255604751135 1102 26-27 0.7719124015969854 1102 28-29 0.7590651983976855 1102 30-31 0.8136689372070052 1102 32-33 0.8140510862643069 1102 34-35 0.6613724712580904 1102 36-37 0.830187135414846 1102 38-39 0.8212428165769055 1102 40-41 0.8072829882883994 1102 42-43 0.5720762998747375 1102 44-45 1.0953817100059524 1102 46-47 0.901275919924899 1102 48-49 0.7936082170720695 1102 50-51 0.8330171012915599 1102 52-53 0.6912113458222819 1102 54-55 0.7341213705609633 1102 56-57 0.900232508648763 1102 58-59 0.5561171204298603 1102 60-61 0.6928447900435941 1102 62-63 0.6693847757521993 1102 64-65 0.6044958187489762 1102 66-67 0.6829396568240576 1102 68-69 0.4873572950869658 1102 70-71 0.6206369468742743 1102 72-73 0.42057894054335776 1102 74-75 0.47886426517974456 1102 76-77 0.616880993671014 1102 78-79 0.3117379261631221 1102 80-81 0.33233699619419 1102 82-83 0.2783630604220484 1102 84-85 0.07742182164338018 1102 86-87 0.14242122608441932 1102 88-89 0.378941422676057 1102 90-91 0.1323192483447393 1102 92-93 0.15733056409117552 1102 94-95 -0.0417864950016309 1102 96-97 -0.05161546614759516 1102 98-99 -0.12991021708937112 1102 100-101 -0.27247270350354924 1103 1 0.024803679792828603 1103 2 0.3721205396221201 1103 3 0.2900709190888975 1103 4 0.12926233925906416 1103 5 0.07677143041286882 1103 6 0.14473733985256843 1103 7 0.02884394964506498 1103 8 0.1008470738393683 1103 9 0.30344228130122985 1103 10-11 0.11850702238887578 1103 12-13 0.29229884891532265 1103 14-15 0.4395069703762786 1103 16-17 0.4114072126607198 1103 18-19 0.3751542202651521 1103 20-21 0.2545373047825521 1103 22-23 0.3003493278885827 1103 24-25 0.36032547078955446 1103 26-27 0.24971977156808123 1103 28-29 -0.013802345733061827 1103 30-31 0.1408591130028114 1103 32-33 0.14003308249508706 1103 34-35 0.3995073147022623 1103 36-37 0.4289457782542385 1103 38-39 0.27853840319921375 1103 40-41 0.3304762485401156 1103 42-43 0.30183872405105916 1103 44-45 0.13921779753420083 1103 46-47 0.37025638301746966 1103 48-49 0.5326467333821086 1103 50-51 0.42186791421839587 1103 52-53 0.3209614988331069 1103 54-55 0.23428801601821192 1103 56-57 0.5334844886997843 1103 58-59 0.4127755154220125 1103 60-61 0.5606911550516855 1103 62-63 0.48780940049692845 1103 64-65 0.29674256310272185 1103 66-67 0.07267641759996124 1103 68-69 0.22574362167570072 1103 70-71 0.11682985565558113 1103 72-73 0.3228709215639576 1103 74-75 -0.028770369200124435 1103 76-77 -0.11608253824310566 1103 78-79 0.0125729713997913 1103 80-81 -0.022789018070657363 1103 82-83 -0.21833584456580368 1103 84-85 -0.045910208825468146 1103 86-87 -0.21391978505961617 1103 88-89 -0.4817278254802382 1103 90-91 -0.5570028682343597 1103 92-93 -0.36772912528169144 1103 94-95 -0.7886293210661925 1103 96-97 -0.6381430899045668 1103 98-99 -0.8542453017149647 1103 100-101 -0.8714138988665603 1104 1 0.011463886317368832 1104 2 0.09706398837799313 1104 3 0.11742312083733708 1104 4 0.26900541360669905 1104 5 0.2929366860484919 1104 6 0.24949214445827295 1104 7 -0.025113737424049987 1104 8 0.030053016370104046 1104 9 0.1988359799626025 1104 10-11 0.13049828619667636 1104 12-13 0.2866204739910714 1104 14-15 0.3648908288211601 1104 16-17 0.23291228577232914 1104 18-19 0.07898245448630092 1104 20-21 0.15512411769243073 1104 22-23 0.09814037929171349 1104 24-25 0.1628858771184909 1104 26-27 0.2669311502184115 1104 28-29 0.11648377056150139 1104 30-31 0.4010338368467359 1104 32-33 0.17000224181659718 1104 34-35 0.28753660134655945 1104 36-37 0.36395609848128885 1104 38-39 0.250721041217254 1104 40-41 0.036552282212859666 1104 42-43 0.21204149851209309 1104 44-45 0.3952524302961251 1104 46-47 0.1497012808052176 1104 48-49 0.24108756908049855 1104 50-51 0.11888149851725416 1104 52-53 0.026346017292517843 1104 54-55 -0.07350000531825884 1104 56-57 0.13930806681850072 1104 58-59 0.24244226809057068 1104 60-61 0.32469171306396305 1104 62-63 0.23258478555539597 1104 64-65 0.24863350470343804 1104 66-67 0.1505063798591273 1104 68-69 0.03996678458528535 1104 70-71 -0.04666999134105154 1104 72-73 -0.041225338553768864 1104 74-75 -0.13165763271992148 1104 76-77 -0.46614598963903475 1104 78-79 -0.5398569320580862 1104 80-81 -0.5946383487559324 1104 82-83 -0.6934435469352529 1104 84-85 -0.6961977651517053 1104 86-87 -0.44475986354134633 1104 88-89 -0.6569811810540607 1104 90-91 -0.7189171803492265 1104 92-93 -0.764510354468733 1104 94-95 -0.5389803287883588 1104 96-97 -0.45710035696444606 1104 98-99 -0.3315498024139778 1104 100-101 -0.5705635301584486 1105 1 0.0533999961845133 1105 2 0.09702069833470262 1105 3 0.07239550477524048 1105 4 0.20294779306632194 1105 5 -0.047148401277972596 1105 6 0.17756648391192442 1105 7 0.16328900925456225 1105 8 0.14420587500572424 1105 9 0.21479060005515294 1105 10-11 0.20834572611308744 1105 12-13 0.07148314006063572 1105 14-15 0.2806319842174929 1105 16-17 0.20814142444974237 1105 18-19 0.3230957549789153 1105 20-21 0.3949270733574579 1105 22-23 0.4582314376586325 1105 24-25 0.10891588159677212 1105 26-27 0.18373962909586083 1105 28-29 0.1918987578730409 1105 30-31 0.2119638263974153 1105 32-33 0.3230176172457604 1105 34-35 0.41771349342000974 1105 36-37 0.27569143211317026 1105 38-39 0.12525231454162622 1105 40-41 0.25622238222779004 1105 42-43 0.14233184035416002 1105 44-45 0.351729516428378 1105 46-47 0.5108439886720646 1105 48-49 0.17230790047324263 1105 50-51 0.11635948030557586 1105 52-53 -0.022013439342806862 1105 54-55 -0.050736159968209904 1105 56-57 0.09231075378670539 1105 58-59 0.13395294132538993 1105 60-61 0.1607620220653061 1105 62-63 0.17676152835283432 1105 64-65 0.17583457949071857 1105 66-67 0.12421962064133751 1105 68-69 0.0016901761362611012 1105 70-71 -0.20882031228447673 1105 72-73 3.2340006393738463E-4 1105 74-75 -0.02039625042405646 1105 76-77 0.09267249073116801 1105 78-79 -0.010822001471431975 1105 80-81 0.0309303928472886 1105 82-83 0.13946211062557623 1105 84-85 0.03760951951764824 1105 86-87 0.22066184119070442 1105 88-89 0.20113047740242607 1105 90-91 0.2777308268160219 1105 92-93 0.2450985423815446 1105 94-95 0.3266757392815016 1105 96-97 -0.022725823451978044 1105 98-99 0.1336718724628696 1105 100-101 0.26964172435714673 1106 1 0.06357063460757928 1106 2 0.1339918663252888 1106 3 0.13966234796441057 1106 4 0.22251825496687871 1106 5 0.08626730922511427 1106 6 0.06744733121995239 1106 7 0.0688171057535314 1106 8 -0.06076911763917536 1106 9 0.034809723326702624 1106 10-11 0.23451151016721639 1106 12-13 0.14044239278202753 1106 14-15 0.12724495862173058 1106 16-17 0.12436752036030185 1106 18-19 0.3171006093478468 1106 20-21 0.17837367529920556 1106 22-23 0.2597723320393328 1106 24-25 0.10661226288538472 1106 26-27 0.014494998322099661 1106 28-29 0.11411337982067238 1106 30-31 -0.020856856156278525 1106 32-33 0.06263220977297834 1106 34-35 0.09931322863624814 1106 36-37 -0.06871942402098341 1106 38-39 0.1098845593195037 1106 40-41 0.1551794284178456 1106 42-43 0.05584978680899866 1106 44-45 0.02255108159461372 1106 46-47 0.012081117239290506 1106 48-49 -0.04633949581978669 1106 50-51 -0.016431046319901554 1106 52-53 -0.23679425723041447 1106 54-55 -0.1975793491414919 1106 56-57 0.228294866761118 1106 58-59 0.01666329731244076 1106 60-61 -0.21110029037953382 1106 62-63 -0.12333850106758604 1106 64-65 -0.33227662968845095 1106 66-67 -0.1763923240482761 1106 68-69 0.046273877224820126 1106 70-71 -0.07096873829212669 1106 72-73 -0.16554082470805653 1106 74-75 -0.49881710950025493 1106 76-77 -0.5063529579302006 1106 78-79 -0.30353824154203934 1106 80-81 -0.1506847880882809 1106 82-83 -0.11425515915965434 1106 84-85 -0.3327089565047139 1106 86-87 -0.22769723500817562 1106 88-89 -0.2611348947658598 1106 90-91 -0.22691612817073903 1106 92-93 -0.07214402896297045 1106 94-95 0.21876753098494106 1106 96-97 0.24373122273807724 1106 98-99 0.23740973654053832 1106 100-101 0.3311384881111934 1107 1 0.17162843441295195 1107 2 0.13860577991978218 1107 3 0.2540738264535598 1107 4 0.24005735017588137 1107 5 0.1045532304236545 1107 6 0.09523548158092154 1107 7 -0.07927509330954052 1107 8 -0.020502749702899337 1107 9 -0.01010450483995129 1107 10-11 0.02944129720865618 1107 12-13 0.1435810421585373 1107 14-15 0.11210051568602353 1107 16-17 0.20636986267818003 1107 18-19 0.31845706034021504 1107 20-21 0.15718814561852668 1107 22-23 0.2414482208754194 1107 24-25 0.24194618462707496 1107 26-27 0.15938065473688567 1107 28-29 0.13203861801289918 1107 30-31 0.24909669353028363 1107 32-33 0.47674722097536915 1107 34-35 0.5883894840959982 1107 36-37 0.5445958610176049 1107 38-39 0.8098443891337013 1107 40-41 0.8168983729037862 1107 42-43 0.6424717004940206 1107 44-45 0.8552493299481938 1107 46-47 0.6843404921685732 1107 48-49 0.6233102314755712 1107 50-51 0.6756135595596575 1107 52-53 0.33034087301150805 1107 54-55 0.3472358680038212 1107 56-57 0.40037602185197585 1107 58-59 0.3796872070596464 1107 60-61 0.4237923250956044 1107 62-63 0.523871085462396 1107 64-65 0.5943194279755666 1107 66-67 0.6588816253033407 1107 68-69 0.8480538124998986 1107 70-71 1.0196645362003736 1107 72-73 0.6765238662644038 1107 74-75 0.9431468731190691 1107 76-77 1.0544440525027312 1107 78-79 0.8394577188082906 1107 80-81 0.8962916982085964 1107 82-83 0.9332616444251087 1107 84-85 0.8129008948090224 1107 86-87 0.6801956407245058 1107 88-89 0.6643239505959002 1107 90-91 0.8230222021073956 1107 92-93 1.0591544864374889 1107 94-95 1.0763727089784716 1107 96-97 1.0571110063848508 1107 98-99 1.1256998444908426 1107 100-101 1.220434265149688 1108 1 -0.0975076885514774 1108 2 0.08521446898786778 1108 3 -0.19655056848637997 1108 4 -0.07740515638175083 1108 5 0.16679802352691553 1108 6 0.1350058426969909 1108 7 0.09166417623761447 1108 8 0.009957122984346256 1108 9 -0.23386720772098357 1108 10-11 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-2.3080925769649774 2316 68-69 -2.4458887645309098 2316 70-71 -2.3667945929058156 2316 72-73 -2.3039209340333393 2316 74-75 -2.3047596321403816 2316 76-77 -2.138614114820143 2316 78-79 -2.2459385026173457 2316 80-81 -2.038748751537735 2316 82-83 -2.1525496031508453 2316 84-85 -2.0563107758732357 2316 86-87 -2.454358530594373 2316 88-89 -2.632223864334268 2316 90-91 -2.588031205269541 2316 92-93 -2.5933461991514335 2316 94-95 -2.3467715487246146 2316 96-97 -2.2519243210328312 2316 98-99 -2.6325676233354507 2316 100-101 -2.7062126173795455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5353.0 3 1233.0 4 1138.0 5 1279.0 6 1458.0 7 1732.0 8 2155.0 9 2685.0 10 3346.0 11 4413.0 12 6049.0 13 7770.0 14 8045.0 15 8761.0 16 10736.0 17 13506.0 18 11275.0 19 7749.0 20 7594.0 21 8093.0 22 8750.0 23 10156.0 24 11173.0 25 12501.0 26 14001.0 27 15640.0 28 18061.0 29 20809.0 30 23932.0 31 27878.0 32 33214.0 33 40747.0 34 51635.0 35 70904.0 36 95299.0 37 134484.0 38 124818.0 39 20239.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63327573834882 20.25797360072513 14.79825232268298 21.310498338243068 2 15.936006869212505 22.103554793882342 39.48664902971194 22.47378930719321 3 18.52370776074763 27.173584870056654 31.813003297527565 22.489704071668154 4 12.80460689359062 18.225987381056015 38.00356935170146 30.96583637365191 5 12.69311857819894 37.94462252876058 34.45382910119266 14.908429791847816 6 28.540980727087113 36.751036498174564 19.168349465807054 15.539633308931272 7 25.218682950982007 35.227170914578075 22.675358970127082 16.87878716431283 8 24.926169701814015 33.69267025767806 22.052063770514803 19.329096269993126 9 26.594830381089924 16.493147454979514 22.472457190014442 34.43956497391612 10-11 23.526204444832175 27.46782026623251 28.463524919436463 20.54245036949885 12-13 25.141916339212766 26.113726419487605 28.585988627829632 20.15836861347 14-15 22.52357718976521 26.878497378936828 28.395902264082206 22.202023167215753 16-17 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27.448315685656187 31.96715443556522 20.38890956346922 42-43 20.14343484917621 27.603396368676563 31.44103020263516 20.812138579512062 44-45 20.04866545841536 27.78979978620818 31.196381875283368 20.965152880093093 46-47 20.214962243401324 27.825766821781038 30.778464237475333 21.180806697342298 48-49 20.312656323721896 27.999121479182786 30.387703550135775 21.300518646959542 50-51 20.270805401901853 28.27664177624667 30.047383546278127 21.405169275573353 52-53 20.301030507960864 28.46020515443412 29.86418937478097 21.37457496282405 54-55 20.09378349886322 28.24236440488853 30.06754377086141 21.596308325386833 56-57 20.15038030025168 28.520536450298223 29.69336313481264 21.63572011463746 58-59 20.146998006776602 28.01659185423023 29.896990167592875 21.93941997140029 60-61 20.214288037901124 27.9195488299329 30.072247540241847 21.793915591924126 62-63 20.230788305763138 27.66317075292643 30.178607043789768 21.927433897520665 64-65 20.064781249315452 27.65553094633789 30.16537512677102 22.114312677575633 66-67 20.036707350646967 27.905164783441432 29.68416877092541 22.37395909498619 68-69 20.0339161487171 27.64610204094925 29.762959989247257 22.557021821086394 70-71 19.83300343360193 27.64911063256078 29.791633237550897 22.72625269628639 72-73 19.97772733569914 27.420538925062726 29.635846082983143 22.965887656254996 74-75 19.722100367576758 27.57553853339893 29.520161073094435 23.18220002592988 76-77 19.687536068897142 27.426804184731946 29.750898947898012 23.134760798472897 78-79 19.750834929028073 27.193478677652305 29.73453872390181 23.32114766941781 80-81 19.627658661661247 27.24538301356115 29.624220590436956 23.502737734340652 82-83 19.725575328294006 27.02107662915975 29.53367691655757 23.71967112598867 84-85 19.634874800774355 26.662650096143203 29.596719353576773 24.105755749505672 86-87 19.76527730672638 26.392807740592833 29.73886094148328 24.103054011197507 88-89 19.9823238093434 25.999391455994807 29.861396407222497 24.156888327439297 90-91 19.890373752215552 25.94255011561727 29.917108396075285 24.249967736091886 92-93 20.05632325308523 25.768509890609515 29.79845818498702 24.37670867131824 94-95 19.952139966325102 25.390209651102484 29.88173527552081 24.7759151070516 96-97 20.120372579538436 25.05486685426286 29.774274771785265 25.05048579441344 98-99 20.135425694651644 24.856203439277476 29.78737763380362 25.220993232267265 100-101 20.468042563745286 24.388739560760236 30.325395890539887 24.81782198495459 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2460.0 1 1615.0 2 931.0 3 1046.0 4 865.0 5 851.0 6 1264.5 7 2035.0 8 2845.5 9 3128.5 10 2735.0 11 2319.0 12 2357.5 13 2450.0 14 2485.0 15 2617.0 16 2710.5 17 2816.5 18 2915.5 19 3039.5 20 3274.0 21 3574.0 22 3965.0 23 4445.0 24 4931.5 25 5548.5 26 6600.5 27 7865.0 28 8864.0 29 10141.5 30 11727.0 31 13200.5 32 14894.0 33 16848.0 34 19162.5 35 20971.0 36 22573.0 37 24736.5 38 25720.0 39 25587.0 40 25649.5 41 26150.5 42 26827.0 43 28010.0 44 29047.5 45 29354.5 46 29447.0 47 29975.5 48 31434.0 49 31352.0 50 29608.5 51 27913.5 52 26047.5 53 24537.0 54 23041.0 55 20867.5 56 18716.5 57 16360.0 58 14433.0 59 12805.0 60 10899.0 61 9252.0 62 7527.5 63 5617.5 64 4018.0 65 3013.5 66 2250.5 67 1708.0 68 1305.5 69 950.5 70 726.0 71 591.0 72 482.5 73 427.5 74 366.0 75 301.0 76 242.5 77 145.5 78 86.5 79 64.0 80 40.0 81 34.0 82 29.5 83 19.0 84 14.0 85 11.0 86 7.5 87 5.0 88 3.5 89 3.0 90 2.5 91 2.0 92 1.0 93 1.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15531207356937313 2 0.09108970627399501 3 0.08319447946887512 4 0.09910277228516146 5 0.1193711157251707 6 0.03829774196513373 7 0.04171507894048413 8 0.045368094327927655 9 0.045839451152113915 10-11 0.04365942584025246 12-13 0.04748920003676583 14-15 0.0458983707551372 16-17 0.0445432198856017 18-19 0.048431913685138356 20-21 0.049845984157697136 22-23 0.0625726184107262 24-25 0.07859875043305908 26-27 0.08119121296608352 28-29 0.06952513156747356 30-31 0.0591552814353758 32-33 0.04860867249420821 34-35 0.29053256250780685 36-37 0.4631080797630018 38-39 0.439599158156712 40-41 0.4133210152083279 42-43 0.4443127263985746 44-45 0.45568420978206814 46-47 0.45497717454578873 48-49 0.4728298142618434 50-51 0.4609280544511403 52-53 0.4692357184774232 54-55 0.4598675015967213 56-57 0.4347677507088028 58-59 0.41249614076600194 60-61 0.38374337449064005 62-63 0.4443127263985746 64-65 0.4807250410669633 66-67 0.4827283075697549 68-69 0.49239112246557326 70-71 0.4908592127869679 72-73 0.4809017998760332 74-75 0.47471524155858846 76-77 0.4568036822395105 78-79 0.42799199636112534 80-81 0.43170393135159213 82-83 0.4468462693285758 84-85 0.44549111845904027 86-87 0.4563323254153243 88-89 0.4684108440350972 90-91 0.473360090689053 92-93 0.47430280433742544 94-95 0.4793698901974278 96-97 0.4795466490064976 98-99 0.47347792989509957 100-101 0.46010318000881434 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 848614.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.45039243284697 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75902293815362 56.322703364649854 2 9.513615901915692 12.643670202753421 3 2.485529992703324 4.954933302562414 4 1.0193757720950107 2.7095168036899935 5 0.5398713112068558 1.793733024646561 6 0.3243025292171907 1.2930018200068285 7 0.20234446427960046 0.9412108340595544 8 0.18030209509540843 0.9584915980443504 9 0.12628532068358828 0.7552538206139129 >10 0.7671066312998098 9.5695035815248 >50 0.05884774914555966 2.6931070597232183 >100 0.021445686389833133 2.3544346823735207 >500 5.317112221599628E-4 0.21223006570335903 >1k 0.0010634224443199255 1.3555871566291136 >5k 3.544741481066419E-4 1.442622683019115 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6773 0.7981249425533872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5476 0.6452874923109918 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3525 0.41538320131414286 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2614 0.3080316846057218 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1927 0.22707615005173143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1257 0.14812388200053264 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1167 0.13751835345634175 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1020 0.12019599016749666 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.891960302328267E-5 0.0 20-21 0.0 0.0 0.0 7.070352362793921E-4 0.0 22-23 0.0 0.0 0.0 0.002533542930001155 0.0 24-25 0.0 0.0 0.0 0.006245477920467963 0.0 26-27 0.0 0.0 0.0 0.011725001001633251 0.0 28-29 0.0 0.0 0.0 0.0316398268235028 0.0 30-31 0.0 0.0 0.0 0.08649397723817896 0.0 32-33 0.0 0.0 0.0 0.204686700902884 0.0 34-35 0.0 0.0 0.0 0.3992392300857634 0.0 36-37 0.0 0.0 0.0 0.6917750590963618 0.0 38-39 0.0 0.0 0.0 1.068683759636301 0.0 40-41 0.0 0.0 0.0 1.4635040194953182 0.0 42-43 0.0 0.0 0.0 1.8520198818308442 0.0 44-45 0.0 0.0 0.0 2.334571430591529 0.0 46-47 0.0 0.0 0.0 2.9086840424503952 0.0 48-49 0.0 0.0 0.0 3.476727935197864 0.0 50-51 0.0 0.0 0.0 4.045596702387658 0.0 52-53 0.0 0.0 0.0 4.588658683453254 0.0 54-55 0.0 0.0 0.0 5.1875175285819 0.0 56-57 0.0 0.0 0.0 5.891783543519198 0.0 58-59 0.0 0.0 0.0 6.618792525223482 0.0 60-61 0.0 0.0 0.0 7.3864560330138325 0.0 62-63 0.0 0.0 0.0 8.146106474793015 0.0 64-65 0.0 0.0 0.0 8.941933552828495 0.0 66-67 0.0 0.0 0.0 9.805989531164935 0.0 68-69 0.0 0.0 0.0 10.749528053979784 0.0 70-71 0.0 0.0 0.0 11.754696481556985 0.0 72-73 0.0 0.0 0.0 12.747609631705345 0.0 74-75 0.0 0.0 0.0 13.757432707921387 0.0 76-77 0.0 0.0 0.0 14.837311192132113 0.0 78-79 0.0 0.0 0.0 15.968744328988208 0.0 80-81 0.0 0.0 0.0 17.159568425691774 0.0 82-83 0.0 0.0 0.0 18.335073425609288 0.0 84-85 0.0 0.0 0.0 19.508339480611916 0.0 86-87 0.0 0.0 0.0 20.732158555008517 0.0 88-89 0.0 0.0 0.0 22.001227884527005 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3865 0.0 40.95278 1 GTATCAA 6370 0.0 35.933056 1 TATCAAC 7860 0.0 29.166117 2 ATCAACG 7895 0.0 29.036814 3 TCAACGC 7985 0.0 28.709541 4 CAACGCA 8065 0.0 28.424759 5 AACGCAG 8225 0.0 27.852095 6 GTACATG 5160 0.0 26.358374 1 TACATGG 5240 0.0 25.671001 2 ACATGGG 5215 0.0 24.885826 3 ACGCAGA 9160 0.0 24.752216 7 CGCAGAG 9215 0.0 24.347649 8 CATGGGG 1285 0.0 23.958853 4 TAAGGCG 120 1.7436694E-4 23.682404 5 GAGTACT 5215 0.0 23.373436 12-13 GCAGAGT 9560 0.0 22.874842 9 GTGCTAA 150 3.0867704E-5 22.11532 1 AGTACTT 5400 0.0 21.871393 12-13 CATGGGA 3700 0.0 21.634197 4 GTACTTT 5970 0.0 20.49679 14-15 >>END_MODULE