FastQCFastQC Report
Thu 26 May 2016
SRR938753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848614
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63950.7535817226677853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55330.652004327055646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36800.43364827825136043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26280.3096814334903737No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT23710.2793967575364064No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.21116785723544507No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT13790.1625002651382136RNA PCR Primer, Index 17 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.14223192169820437No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.13681131822006234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42600.042.515811
GTATCAA66100.033.385351
CATTCCG1004.9859635E-528.47119
GGTATAC700.00825175627.2356871
ATCAACG83550.026.466253
TATCAAC84350.026.1589832
TCAACGC84700.026.0508884
CAACGCA85950.025.672025
AACGCAG87750.025.1454146
CGTAACG601.7224075E-423.72731414-15
GAGTACT55450.022.33662212-13
TAACGGA753.056781E-522.14549416-17
ACGCAGA99100.022.1218457
CGCAGAG100000.021.9227458
AGTACTT56350.021.13773212-13
GTAGGAC1353.8209016E-421.0897053
GCAGAGT103450.020.9622889
GTACTTT59800.020.55309114-15
GTACATG53850.020.1802021
CATGGGA35800.019.7495064
Produced by FastQC (version 0.11.5)