##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938753_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 848614 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912345306582264 31.0 31.0 34.0 30.0 34.0 2 32.129560671872014 33.0 31.0 34.0 30.0 34.0 3 32.198119521949906 34.0 31.0 34.0 30.0 34.0 4 35.87452952696986 37.0 35.0 37.0 35.0 37.0 5 35.78480439870188 37.0 35.0 37.0 35.0 37.0 6 35.81872441416239 37.0 35.0 37.0 33.0 37.0 7 35.743423983106574 37.0 35.0 37.0 33.0 37.0 8 35.721032177173605 37.0 35.0 37.0 33.0 37.0 9 37.33113641773527 39.0 37.0 39.0 34.0 39.0 10-11 37.27953816458366 39.0 37.0 39.0 33.0 39.0 12-13 37.21049558456495 39.0 37.0 39.0 33.0 39.0 14-15 38.38339162446059 40.0 38.0 41.0 33.0 41.0 16-17 38.32180060663623 40.0 38.0 41.0 33.0 41.0 18-19 38.37196004308201 40.0 38.0 41.0 33.5 41.0 20-21 38.43165974164933 40.0 38.0 41.0 34.0 41.0 22-23 38.35431303278051 40.0 38.0 41.0 33.5 41.0 24-25 38.26659883056372 40.0 38.0 41.0 33.0 41.0 26-27 38.07132866061602 40.0 37.0 41.0 33.0 41.0 28-29 37.88217611305022 40.0 37.0 41.0 33.0 41.0 30-31 37.57778624910737 40.0 36.5 41.0 32.5 41.0 32-33 37.278205992359304 39.0 36.0 41.0 32.0 41.0 34-35 37.13000021211057 39.5 35.0 41.0 31.0 41.0 36-37 36.96884331392129 40.0 35.0 41.0 31.0 41.0 38-39 36.8070547975876 39.0 35.0 41.0 31.0 41.0 40-41 36.58635198099489 39.0 35.0 41.0 30.0 41.0 42-43 36.32971174173417 39.0 35.0 41.0 30.0 41.0 44-45 35.96195266634771 39.0 35.0 41.0 29.5 41.0 46-47 35.50836010247298 39.0 35.0 41.0 26.5 41.0 48-49 35.11140930976863 39.0 35.0 41.0 25.0 41.0 50-51 34.8150160143481 39.0 35.0 40.5 24.0 41.0 52-53 34.58662536795292 38.5 34.0 40.0 23.5 41.0 54-55 34.32612884067432 38.0 34.0 40.0 23.0 41.0 56-57 34.0440188354187 38.0 34.0 40.0 22.0 41.0 58-59 33.70768511950074 38.0 33.5 40.0 20.0 41.0 60-61 33.350209282429944 37.0 33.0 40.0 18.5 41.0 62-63 32.953187786201966 37.0 33.0 40.0 14.0 41.0 64-65 32.56904493680283 36.0 32.0 39.5 10.0 41.0 66-67 32.14557325238565 36.0 32.0 39.0 7.5 40.5 68-69 31.674994756155332 35.0 31.0 39.0 2.0 40.0 70-71 31.20649258673555 35.0 31.0 38.0 2.0 40.0 72-73 30.87391853068651 35.0 31.0 37.5 2.0 39.5 74-75 30.451883306191036 35.0 30.5 37.0 2.0 39.0 76-77 28.42375037413948 32.5 27.0 35.0 2.0 37.5 78-79 29.39669684921531 34.0 29.0 36.0 2.0 38.0 80-81 29.408413601472517 34.0 30.0 35.5 2.0 37.0 82-83 29.092451338299863 34.0 29.0 35.0 2.0 37.0 84-85 28.708432220067074 34.0 29.0 35.0 2.0 36.5 86-87 28.174920517455522 34.0 28.5 35.0 2.0 36.0 88-89 27.663587921010024 34.0 27.0 35.0 2.0 35.5 90-91 27.254507349631282 33.5 26.0 35.0 2.0 35.0 92-93 26.96862766817422 33.0 26.0 35.0 2.0 35.0 94-95 26.59636418913664 33.0 25.0 35.0 2.0 35.0 96-97 26.10938129703257 33.0 24.5 35.0 2.0 35.0 98-99 25.425130860438315 33.0 21.0 35.0 2.0 35.0 100-101 22.762317732208047 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.014037559302519043 1101 2 0.06634952054411514 1101 3 0.12719644912961314 1101 4 -0.02136000887686862 1101 5 0.07201630576300033 1101 6 0.1870533142036166 1101 7 0.2133306610969612 1101 8 0.1725359001855864 1101 9 0.1245314488812923 1101 10-11 0.03310857754200214 1101 12-13 0.1463896231097479 1101 14-15 0.25025556936799376 1101 16-17 0.28294112219752776 1101 18-19 0.227958464142624 1101 20-21 0.36274535179375533 1101 22-23 0.21166073682930886 1101 24-25 0.1387288389151351 1101 26-27 0.16271081223704442 1101 28-29 0.20818720503051935 1101 30-31 0.16788741407678742 1101 32-33 0.33679991566805256 1101 34-35 0.32049621977311205 1101 36-37 0.43859818045460486 1101 38-39 0.5428959917278462 1101 40-41 0.515923359472815 1101 42-43 0.09353173480232613 1101 44-45 0.29468521652736257 1101 46-47 0.6368856750789362 1101 48-49 0.5057321354336111 1101 50-51 0.4518528662188359 1101 52-53 0.5273849539185136 1101 54-55 0.3159849422823413 1101 56-57 0.4170440407825282 1101 58-59 0.5798383232517565 1101 60-61 0.5247158905692544 1101 62-63 0.5857673353425525 1101 64-65 0.45508452544722644 1101 66-67 0.5921620391023339 1101 68-69 0.8577678140646725 1101 70-71 0.4467960336494805 1101 72-73 0.49175005922315407 1101 74-75 0.6787839274886309 1101 76-77 0.8006350991533076 1101 78-79 0.5555535227866244 1101 80-81 1.0827483730120342 1101 82-83 1.0957794208683218 1101 84-85 1.0097038367186677 1101 86-87 0.7175394580246959 1101 88-89 0.9438913496179175 1101 90-91 0.7942756209238837 1101 92-93 0.8015701075404458 1101 94-95 0.7849626061732167 1101 96-97 0.8795902792419064 1101 98-99 0.8564512485974944 1101 100-101 0.9586435368426827 1102 1 0.1903035843410521 1102 2 0.22570701518677083 1102 3 0.21772930124184597 1102 4 0.20062202052272937 1102 5 0.2689660657790398 1102 6 0.22921655342313585 1102 7 0.16156179760584877 1102 8 0.24459061402194493 1102 9 0.2916993875255969 1102 10-11 0.27347788967153264 1102 12-13 0.30418377577213107 1102 14-15 0.3853953615907528 1102 16-17 0.5072227367945672 1102 18-19 0.3877279416919066 1102 20-21 0.4542212218139312 1102 22-23 0.4285313585318846 1102 24-25 0.3221489549596015 1102 26-27 0.38862939313607825 1102 28-29 0.44388998834318016 1102 30-31 0.4882315372894368 1102 32-33 0.44296932053289595 1102 34-35 0.44489469339993803 1102 36-37 0.48577727240041924 1102 38-39 0.3775576958019329 1102 40-41 0.49286484850122037 1102 42-43 0.45336512452875155 1102 44-45 0.24731188169021578 1102 46-47 0.12837658325843648 1102 48-49 0.14799468916369563 1102 50-51 0.19736648201892848 1102 52-53 0.11566834803107184 1102 54-55 0.01835157260679665 1102 56-57 -0.005214941384721783 1102 58-59 -0.017497729482052193 1102 60-61 -0.21438927629165505 1102 62-63 -0.33795160145957936 1102 64-65 -0.09284804093997678 1102 66-67 0.15164109219956856 1102 68-69 0.31437959144978933 1102 70-71 0.3130989648373692 1102 72-73 0.22619339669197913 1102 74-75 0.11030390944283752 1102 76-77 0.006313257480059065 1102 78-79 0.018890177046809953 1102 80-81 0.11854859106534477 1102 82-83 0.1650802717775086 1102 84-85 0.4032086050683539 1102 86-87 0.2052676934220159 1102 88-89 0.33636537746357575 1102 90-91 0.6383017121303212 1102 92-93 0.7158040386406128 1102 94-95 0.6580875356818439 1102 96-97 0.6718130445087702 1102 98-99 0.9132657278385707 1102 100-101 1.1838070768253885 1103 1 -0.076171591545652 1103 2 0.04556176094639852 1103 3 -0.1951802678433019 1103 4 0.022462877140981163 1103 5 0.02041891117447392 1103 6 0.025942424675889697 1103 7 0.03552329341547278 1103 8 0.05988836400528186 1103 9 -0.13175580908573892 1103 10-11 0.009044878115432198 1103 12-13 0.040043151245555464 1103 14-15 -0.059900994630581295 1103 16-17 -0.13688770975466014 1103 18-19 -0.0032503277404742903 1103 20-21 -0.019074601141930714 1103 22-23 -0.14365869606722725 1103 24-25 0.012198251715773267 1103 26-27 0.03618712821221237 1103 28-29 0.11599914414759382 1103 30-31 0.05631984120227429 1103 32-33 0.13573115084540177 1103 34-35 -0.1735425055020201 1103 36-37 -0.10705788191327059 1103 38-39 0.18934377151351356 1103 40-41 0.17450385202198504 1103 42-43 0.21859424688287277 1103 44-45 0.1010335292381015 1103 46-47 0.18180463180983253 1103 48-49 0.3522285442488169 1103 50-51 0.14973466546225467 1103 52-53 0.21343317796181083 1103 54-55 0.13327785471634002 1103 56-57 0.30725135078415633 1103 58-59 0.07929373948250173 1103 60-61 -0.032652557934603976 1103 62-63 -0.2351328692982264 1103 64-65 0.006726407160563497 1103 66-67 0.08316950373506415 1103 68-69 0.4228869935380075 1103 70-71 0.060645489219115944 1103 72-73 -0.09895688721128337 1103 74-75 -0.060383457619536784 1103 76-77 -0.14183000913449106 1103 78-79 -0.15892734314759238 1103 80-81 0.06601891302436869 1103 82-83 0.16969938086059244 1103 84-85 -0.09106333607853045 1103 86-87 -0.022338187041683 1103 88-89 0.17645239669142754 1103 90-91 0.13277968766070813 1103 92-93 0.19666026299993788 1103 94-95 -0.05543983356206894 1103 96-97 0.16668793921914116 1103 98-99 0.262520519652945 1103 100-101 0.7209273524266742 1104 1 -0.01639077636771802 1104 2 -0.04249017619621753 1104 3 -0.060957314938335116 1104 4 -0.196664943671081 1104 5 -0.07154811210003942 1104 6 -0.13090402594603034 1104 7 -0.18484655472118305 1104 8 -0.06308284136123632 1104 9 -0.24403327919008433 1104 10-11 -0.03811990950989497 1104 12-13 -0.02607380332744924 1104 14-15 0.14751456851180933 1104 16-17 0.07466569442023285 1104 18-19 -0.05884155074737407 1104 20-21 -0.008949378387036688 1104 22-23 -0.08593142606729032 1104 24-25 -0.2895401365287711 1104 26-27 -0.3314402095892106 1104 28-29 -0.19303315456297554 1104 30-31 -0.26486872494618297 1104 32-33 -0.3048892928043614 1104 34-35 -0.4283591114673513 1104 36-37 -0.5759614477917196 1104 38-39 -0.5946379760849325 1104 40-41 -0.39709422313566733 1104 42-43 -0.34248934695618516 1104 44-45 -0.4401161260543134 1104 46-47 -0.2681812678799602 1104 48-49 -0.3200284954060635 1104 50-51 -0.3180888766556791 1104 52-53 -0.39605873086018306 1104 54-55 -0.4221123638684716 1104 56-57 -0.40573083482392747 1104 58-59 -0.3419162771378623 1104 60-61 -0.32039863834837234 1104 62-63 -0.4527869076870701 1104 64-65 -0.26913716183795344 1104 66-67 -0.2735053431115624 1104 68-69 -0.08643346151200149 1104 70-71 -0.272645343179196 1104 72-73 -0.22169033734718369 1104 74-75 -0.33895533620086127 1104 76-77 -0.44732372999635217 1104 78-79 -0.27949474563044063 1104 80-81 -0.15615547481216652 1104 82-83 -0.2893287177575772 1104 84-85 -0.13601032491228793 1104 86-87 -0.21590844558736677 1104 88-89 -0.20599976725617708 1104 90-91 -0.3642532470638571 1104 92-93 -0.2919282359470401 1104 94-95 -0.02576843079121005 1104 96-97 -0.24811318640562519 1104 98-99 -0.19967939874526053 1104 100-101 0.12004338297935035 1105 1 0.049462336037120025 1105 2 0.04960118072272479 1105 3 0.04846200137752987 1105 4 -0.08370181484588102 1105 5 0.07465579893145957 1105 6 0.007280777756015766 1105 7 0.07535646065424828 1105 8 0.022098528992550825 1105 9 0.04543977648987152 1105 10-11 0.13304369699853424 1105 12-13 0.2515243456500116 1105 14-15 0.04397971256316424 1105 16-17 0.05972839034499344 1105 18-19 0.09493961061998846 1105 20-21 0.21346590747652527 1105 22-23 0.183816297473534 1105 24-25 0.15328192646570216 1105 26-27 -0.1437383137838708 1105 28-29 0.038994013671086236 1105 30-31 0.10416098098007609 1105 32-33 0.23312309710458123 1105 34-35 0.32486077209735953 1105 36-37 0.1336153547161132 1105 38-39 0.11545457504210077 1105 40-41 0.16656453190240228 1105 42-43 0.1080756627467565 1105 44-45 -0.1853430255915569 1105 46-47 -0.30209379213731324 1105 48-49 -0.24878278709138613 1105 50-51 -0.26400393860522087 1105 52-53 -0.42008470488615757 1105 54-55 -0.08707131296534953 1105 56-57 -0.1453053981915957 1105 58-59 -0.14810152866794368 1105 60-61 -0.3360061917145387 1105 62-63 -0.5506291229430715 1105 64-65 -0.4456635090539578 1105 66-67 -0.4293584555164003 1105 68-69 -0.4151907387175555 1105 70-71 -0.4206301170260396 1105 72-73 -0.6590056082831452 1105 74-75 -0.23973679612025478 1105 76-77 -0.21587127440596632 1105 78-79 -0.26248250499751435 1105 80-81 -0.15012621671393944 1105 82-83 0.006052682451411329 1105 84-85 -0.018876424330109387 1105 86-87 0.23391988699958688 1105 88-89 0.45001530962096936 1105 90-91 0.3363975963456127 1105 92-93 0.11597816799384475 1105 94-95 -0.1136718492118689 1105 96-97 0.14066424306146175 1105 98-99 0.1876687129131973 1105 100-101 0.521489121149223 1106 1 -0.17266917323031095 1106 2 -0.22203606552617217 1106 3 -0.20852534784282994 1106 4 -0.04512135000327788 1106 5 -0.1585142510832469 1106 6 -0.08030380594507136 1106 7 -0.06563353640371616 1106 8 -0.05586704911866036 1106 9 -0.24657405110648511 1106 10-11 0.009165350426009411 1106 12-13 -0.018538172737748937 1106 14-15 -0.03681096116440585 1106 16-17 -0.2808122098315238 1106 18-19 -0.19757068652623389 1106 20-21 -0.14922091217631106 1106 22-23 -0.2736645792549126 1106 24-25 -0.24487635538189778 1106 26-27 -0.17416343882064922 1106 28-29 -0.20987697779699488 1106 30-31 -0.28639653593666026 1106 32-33 -0.17766537009165262 1106 34-35 -0.12968688897942826 1106 36-37 -0.09623680768459764 1106 38-39 -0.18319135023003952 1106 40-41 -0.10011684497315088 1106 42-43 0.01879646298211668 1106 44-45 -0.26692216651536427 1106 46-47 -0.29700258884222563 1106 48-49 -0.30478087476423354 1106 50-51 -0.1389495108323473 1106 52-53 0.06675333571401865 1106 54-55 -0.02672341652522192 1106 56-57 -0.05260239730898775 1106 58-59 -0.0442812874205174 1106 60-61 -0.3541938939799181 1106 62-63 -0.3772303291802075 1106 64-65 -0.15002876354056838 1106 66-67 0.026583733362684825 1106 68-69 0.09241296825508272 1106 70-71 -0.08108907554325029 1106 72-73 -0.055000753914210776 1106 74-75 0.018985622238083977 1106 76-77 -0.07849631118148537 1106 78-79 0.14544188452881812 1106 80-81 0.1402121180904139 1106 82-83 0.13358725144228956 1106 84-85 0.13668624115974026 1106 86-87 0.2689963800857882 1106 88-89 0.2589973631661273 1106 90-91 0.2812791791640876 1106 92-93 0.4886237990618092 1106 94-95 0.44573606488051 1106 96-97 0.34056274262015407 1106 98-99 0.4244567388031655 1106 100-101 0.5528115689279822 1107 1 -0.07967519310041027 1107 2 -0.06652935540780902 1107 3 -0.17647972356419217 1107 4 -0.06048503162909924 1107 5 -0.16501786074219638 1107 6 -0.03980546933023277 1107 7 -0.07639179109400374 1107 8 -0.06643293953892027 1107 9 -0.10439705334695759 1107 10-11 7.593147141520262E-4 1107 12-13 -0.053790339664672615 1107 14-15 -0.14907390049044977 1107 16-17 -0.1215303509137442 1107 18-19 0.10943844511882617 1107 20-21 0.06274329675391499 1107 22-23 -0.03783871418146845 1107 24-25 0.04689498007876125 1107 26-27 0.022135812090255058 1107 28-29 0.20601033068740549 1107 30-31 0.07215631897541641 1107 32-33 0.081794425775918 1107 34-35 0.15418478142137104 1107 36-37 0.2783006694014247 1107 38-39 0.3564569060444214 1107 40-41 0.4446531099909805 1107 42-43 0.29360013827123055 1107 44-45 0.10230266205412875 1107 46-47 0.24867543863191344 1107 48-49 0.08828014997155265 1107 50-51 0.11713825253696797 1107 52-53 0.30648872168726626 1107 54-55 0.20898929309525727 1107 56-57 0.1946946018084006 1107 58-59 0.30168868112226477 1107 60-61 0.17630150059314786 1107 62-63 0.20338020106625265 1107 64-65 0.25200548861504757 1107 66-67 0.12893991278197348 1107 68-69 -0.009899363426178809 1107 70-71 0.08950974311381898 1107 72-73 0.012206512928980118 1107 74-75 0.16040306401960436 1107 76-77 0.29286998433529376 1107 78-79 0.32693474441973436 1107 80-81 0.2602700536823299 1107 82-83 0.35579627219500054 1107 84-85 0.4693986339449516 1107 86-87 0.2533837564634531 1107 88-89 0.324327663933321 1107 90-91 0.412481512429526 1107 92-93 0.4813860934017704 1107 94-95 0.46975472421470243 1107 96-97 0.2354894178866367 1107 98-99 0.06268036792485177 1107 100-101 0.1261278157879211 1108 1 -0.19143620857372312 1108 2 -0.24543784058079865 1108 3 -0.19280214823928787 1108 4 -0.0700605296036656 1108 5 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-0.7720739237836511 2316 58-59 -0.8409382997400101 2316 60-61 -0.9612092729470376 2316 62-63 -0.8918444272466104 2316 64-65 -0.8081475935968641 2316 66-67 -0.9046086465018846 2316 68-69 -1.132325956441008 2316 70-71 -1.092679391281198 2316 72-73 -1.0207703141654996 2316 74-75 -1.242135573813151 2316 76-77 -1.0100048369218797 2316 78-79 -1.1222214915012039 2316 80-81 -1.108451910627874 2316 82-83 -1.1964027542687354 2316 84-85 -1.3516724518320196 2316 86-87 -1.2872826849382832 2316 88-89 -1.28460017293569 2316 90-91 -1.632581267927371 2316 92-93 -1.7523201513338869 2316 94-95 -1.6314443218372858 2316 96-97 -1.7695019143105775 2316 98-99 -2.2465679817646738 2316 100-101 -2.765623814934301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 14.0 5 41.0 6 140.0 7 269.0 8 595.0 9 1027.0 10 1792.0 11 2659.0 12 3682.0 13 5127.0 14 5889.0 15 7646.0 16 9260.0 17 12416.0 18 11412.0 19 7234.0 20 6446.0 21 6493.0 22 7146.0 23 7531.0 24 8497.0 25 9610.0 26 10813.0 27 12717.0 28 15237.0 29 18227.0 30 21933.0 31 26294.0 32 32011.0 33 39500.0 34 51807.0 35 71204.0 36 101755.0 37 160829.0 38 153989.0 39 17365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8612958667441 21.31188262277165 15.417944644675785 21.408876865808466 2 16.145385298852013 22.684518520788014 38.95540257407962 22.21469360628036 3 18.332245284664168 27.33492494820967 32.255654514302144 22.077175252824016 4 12.836696071476549 19.095254143815684 37.34312655695051 30.724923227757262 5 12.530785492579666 38.54956434845525 34.358495146203104 14.561155012761986 6 28.418102930189697 37.47817028707988 18.58088600942242 15.522840773308005 7 25.075240333060734 35.470308055252445 22.12266118635799 17.33179042532883 8 25.071705156879336 34.522173803401785 21.53334731691912 18.872773722799767 9 26.05719443704676 16.7392948973267 22.11959736700078 35.08391329862576 10-11 23.563775756704462 27.798622224002905 28.099819234657925 20.53778278463471 12-13 25.041125882910254 26.12972446836842 28.516204069223463 20.312945579497864 14-15 22.306398794267825 27.09203134997932 28.034628269894522 22.566941585858334 16-17 20.132842563355933 30.13839878340582 28.6659120891682 21.06284656407005 18-19 19.85215875787903 27.958032695704638 32.268301334059224 19.921507212357106 20-21 21.301204081007384 26.937924663038793 32.06734746303973 19.693523792914092 22-23 20.930305179975818 27.49995875627788 31.575427697398347 19.99430836634795 24-25 20.43508591656513 27.721201865630313 32.07188427247253 19.771827945332035 26-27 20.33598413408256 27.83452734260613 32.154702122231434 19.67478640107988 28-29 19.927964893343734 27.819303004664075 32.23797863339516 20.01475346859703 30-31 20.42106305104559 27.705234653211004 32.06899721192438 19.804705083819027 32-33 19.859735993042772 28.18248343769959 31.747178340211214 20.21060222904642 34-35 20.362123195895123 27.930672405076418 31.79449437924179 19.912710019786672 36-37 20.378454572133364 27.806705426310906 31.5342666919669 20.280573309588828 38-39 20.115636092791718 27.63734437682375 31.809766135076913 20.437253395307618 40-41 20.177819836180426 27.56790805079927 31.88100248009706 20.373269632923243 42-43 20.20844086515686 27.64292599689366 31.479249009528793 20.66938412842069 44-45 20.04139754725719 27.98782938035661 31.107022028020236 20.863751044365962 46-47 20.455971591039653 27.840380483039528 30.537216645425936 21.166431280494884 48-49 20.362037606047984 27.933505653633837 30.252759353934387 21.45169738638379 50-51 20.17046760161064 28.30026148735516 30.032335344865174 21.49693556616903 52-53 20.213017932770377 28.45374929001878 29.94105682913551 21.39217594807533 54-55 20.00214467355005 28.29525555788615 29.83453018686941 21.868069581694385 56-57 20.136952725267317 28.671339383983767 29.289111421682883 21.90259646906603 58-59 19.891563979255515 28.445365426561757 29.617103714187067 22.045966879995664 60-61 20.077844992308005 28.64633261824196 29.40038969632086 21.87543269312917 62-63 19.891670417881393 28.553912615158367 29.42509786546062 22.129319101499622 64-65 19.70566123113689 28.49770331387415 29.35963818650175 22.436997268487204 66-67 19.77599760548758 28.66444655771164 28.909495208029156 22.650060628771623 68-69 19.901780558796148 28.538669117733704 28.767926373717017 22.791623949753127 70-71 19.85618657564611 28.469201313458232 28.695927819896195 22.978684290999464 72-73 19.860443028279054 28.375504057203866 28.43949074608715 23.324562168429935 74-75 19.724044147280154 28.57765721517675 28.157089088796557 23.541209548746544 76-77 19.59952393927277 28.549312271538625 28.3874799331439 23.46368385604471 78-79 19.619665993561295 28.50383174959066 28.25409904668946 23.622403210158584 80-81 19.4791383115743 28.545167231706326 28.104271322734864 23.87142313398451 82-83 19.566316369986826 28.416571020511093 27.88075615061736 24.136356458884723 84-85 19.465033572389803 28.17299738161284 27.81853704982477 24.543431996172583 86-87 19.429120697885608 28.167560521832826 27.805966652878737 24.597352127402832 88-89 19.536649912544785 27.870756619139186 27.851486324888885 24.741107143427147 90-91 19.422429511183797 27.84209276246678 27.79848960555095 24.93698812079847 92-93 19.437459198174906 27.77275651827568 27.649143191132836 25.140641092416576 94-95 19.367709799573067 27.47098649738662 27.783731934502576 25.377571768537738 96-97 19.410002663166058 27.43656126342483 27.601005875462814 25.552430197946297 98-99 19.24596826698672 27.546115036150233 27.427097087418684 25.780819609444368 100-101 19.32260951591987 27.327056791138915 27.83194154673027 25.51839214621095 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2008.0 1 1381.0 2 842.5 3 912.0 4 794.0 5 777.0 6 1148.0 7 1895.0 8 2657.5 9 2910.5 10 2423.0 11 1975.5 12 2023.0 13 2066.5 14 2129.5 15 2191.0 16 2288.5 17 2478.0 18 2654.0 19 2846.5 20 3043.5 21 3375.5 22 3824.0 23 4318.0 24 4787.0 25 5492.0 26 6583.5 27 7888.5 28 9000.5 29 10245.0 30 11877.5 31 13385.5 32 15104.5 33 16851.0 34 18840.0 35 20714.5 36 22453.5 37 24471.5 38 25399.5 39 25221.0 40 25425.0 41 26455.0 42 27445.5 43 28588.0 44 29652.0 45 30364.5 46 30632.5 47 30942.5 48 32295.5 49 32200.0 50 30735.5 51 29086.0 52 26725.0 53 24858.0 54 23177.5 55 20884.0 56 18475.5 57 16245.5 58 14557.5 59 12869.0 60 10873.5 61 9004.5 62 7190.0 63 5274.5 64 3675.0 65 2720.5 66 1980.0 67 1464.0 68 1086.5 69 748.5 70 561.5 71 487.0 72 407.5 73 363.5 74 292.0 75 224.5 76 173.5 77 92.0 78 44.5 79 28.0 80 15.0 81 6.0 82 6.0 83 3.5 84 2.0 85 2.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3774389769671488 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1783920604656534E-4 16-17 0.001826507693721763 18-19 1.1783920604656534E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.9459801511641336E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007423869980933616 36-37 0.01614397122837945 38-39 0.010605528544190881 40-41 0.006893593553724073 42-43 0.003299497769303829 44-45 0.0015319096786053493 46-47 0.0010016332513958055 48-49 5.891960302328267E-5 50-51 8.248744423259573E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.5351761813969603E-4 60-61 5.302764272095441E-4 62-63 0.0 64-65 0.0 66-67 8.248744423259573E-4 68-69 4.7135682418626137E-4 70-71 0.0017086684876751975 72-73 0.0 74-75 0.0 76-77 0.09698166657632327 78-79 0.03617663625629556 80-81 1.1783920604656534E-4 82-83 0.0 84-85 0.0 86-87 0.1478292839854162 88-89 0.3243524146431711 90-91 0.2763329381791957 92-93 0.0 94-95 5.891960302328267E-5 96-97 0.0 98-99 2.9459801511641336E-4 100-101 5.302764272095441E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 848614.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.23402885478995 #Duplication Level Percentage of deduplicated Percentage of total 1 83.46177916476867 53.610863311418576 2 10.324890049661315 13.264185707449572 3 2.718943427353965 5.2394607170158825 4 1.097883586674079 2.8208594394249236 5 0.5704380448855282 1.8320766917523497 6 0.34192029440255634 1.3177750834015252 7 0.23256057596120575 1.0456811922745062 8 0.17607061673293842 0.9047780060563386 9 0.13585290093559718 0.7853741242833664 >10 0.8465962872711594 10.108490807264218 >50 0.06576960965103883 2.961570956796112 >100 0.02491396023568113 2.7056066553736247 >500 7.32763528074878E-4 0.3266543740263299 >1k 0.0012823361741310367 1.6730446289288035 >5k 3.66381764037439E-4 1.4035783045338843 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6395 0.7535817226677853 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5533 0.652004327055646 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3680 0.43364827825136043 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2628 0.3096814334903737 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2371 0.2793967575364064 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1792 0.21116785723544507 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1379 0.1625002651382136 RNA PCR Primer, Index 17 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1207 0.14223192169820437 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1161 0.13681131822006234 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1783920604656534E-4 0.0 18-19 0.0 0.0 0.0 1.7675880906984801E-4 0.0 20-21 0.0 0.0 0.0 8.248744423259573E-4 0.0 22-23 0.0 0.0 0.0 0.0027103017390710028 0.0 24-25 0.0 0.0 0.0 0.006481156332561093 0.0 26-27 0.0 0.0 0.0 0.011960679413726381 0.0 28-29 0.0 0.0 0.0 0.032523620868852035 0.0 30-31 0.0 0.0 0.0 0.08867400255004042 0.0 32-33 0.0 0.0 0.0 0.20845755549637407 0.0 34-35 0.0 0.0 0.0 0.40701661768483666 0.0 36-37 0.0 0.0 0.0 0.7062692814400894 0.0 38-39 0.0 0.0 0.0 1.0901894147397992 0.0 40-41 0.0 0.0 0.0 1.4934351778311459 0.0 42-43 0.0 5.891960302328267E-5 0.0 1.8897873473687683 0.0 44-45 0.0 1.1783920604656534E-4 0.0 2.3806465601557365 0.0 46-47 0.0 1.1783920604656534E-4 0.0 2.963832790880188 0.0 48-49 0.0 1.1783920604656534E-4 0.0 3.5384167595632405 1.1783920604656534E-4 50-51 0.0 1.1783920604656534E-4 0.0 4.115416431970248 1.1783920604656534E-4 52-53 0.0 1.1783920604656534E-4 0.0 4.664841730162358 1.1783920604656534E-4 54-55 0.0 1.1783920604656534E-4 0.0 5.271477962890078 1.1783920604656534E-4 56-57 0.0 1.1783920604656534E-4 0.0 5.98481759669296 1.1783920604656534E-4 58-59 0.0 1.1783920604656534E-4 0.0 6.717423940684458 1.1783920604656534E-4 60-61 0.0 1.1783920604656534E-4 0.0 7.491097247983182 1.1783920604656534E-4 62-63 0.0 1.1783920604656534E-4 0.0 8.261471057512603 1.1783920604656534E-4 64-65 0.0 1.1783920604656534E-4 0.0 9.070378287419251 1.1783920604656534E-4 66-67 0.0 1.1783920604656534E-4 0.0 9.947101980405698 1.1783920604656534E-4 68-69 0.0 1.1783920604656534E-4 0.0 10.90866990174567 1.1783920604656534E-4 70-71 0.0 1.1783920604656534E-4 0.0 11.92727199881218 1.1783920604656534E-4 72-73 0.0 1.1783920604656534E-4 0.0 12.938685904309851 1.1783920604656534E-4 74-75 0.0 1.1783920604656534E-4 0.0 13.966891896669157 1.1783920604656534E-4 76-77 0.0 1.1783920604656534E-4 0.0 15.05967377394198 1.1783920604656534E-4 78-79 0.0 1.1783920604656534E-4 0.0 16.207427640835526 1.1783920604656534E-4 80-81 0.0 1.1783920604656534E-4 0.0 17.414218949958403 1.1783920604656534E-4 82-83 0.0 1.1783920604656534E-4 0.0 18.601213272465458 1.1783920604656534E-4 84-85 0.0 1.1783920604656534E-4 0.0 19.784318901172973 1.1783920604656534E-4 86-87 0.0 1.1783920604656534E-4 0.0 21.026520891712842 1.1783920604656534E-4 88-89 0.0 1.1783920604656534E-4 0.0 22.311262835635517 1.1783920604656534E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4260 0.0 42.51581 1 GTATCAA 6610 0.0 33.38535 1 CATTCCG 100 4.9859635E-5 28.4711 9 GGTATAC 70 0.008251756 27.235687 1 ATCAACG 8355 0.0 26.46625 3 TATCAAC 8435 0.0 26.158983 2 TCAACGC 8470 0.0 26.050888 4 CAACGCA 8595 0.0 25.67202 5 AACGCAG 8775 0.0 25.145414 6 CGTAACG 60 1.7224075E-4 23.727314 14-15 GAGTACT 5545 0.0 22.336622 12-13 TAACGGA 75 3.056781E-5 22.145494 16-17 ACGCAGA 9910 0.0 22.121845 7 CGCAGAG 10000 0.0 21.922745 8 AGTACTT 5635 0.0 21.137732 12-13 GTAGGAC 135 3.8209016E-4 21.089705 3 GCAGAGT 10345 0.0 20.962288 9 GTACTTT 5980 0.0 20.553091 14-15 GTACATG 5385 0.0 20.180202 1 CATGGGA 3580 0.0 19.749506 4 >>END_MODULE