FastQCFastQC Report
Thu 26 May 2016
SRR938752_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938752_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843940
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68440.8109581249851885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54930.6508756546673935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34830.4127070644832571No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25940.3073678223570396No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.22430504538237314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.14361210512595682No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.13650259497120648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.11031589923454273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38800.040.040111
GTATCAA61650.034.986621
ATCAACG77400.027.759633
TCAACGC78250.027.4580884
TATCAAC79150.027.1458682
CAACGCA80150.026.8071825
AACGCAG82050.026.125516
GTCGGGA2950.024.1171342
CGGGAGT3200.023.7151834
ACGCAGA91450.023.336397
CGCAGAG92600.022.9953618
GAGTACT51250.022.17753412-13
GTACATG50150.021.9784151
GATAGTC1100.002903326221.5566967
GCAGAGT95900.021.3126669
TACATGG50750.021.3086172
AGTACTT52450.021.17249112-13
ACATGGG50150.020.9015183
GGTCGGG4000.020.191481
TCGGGAG3800.019.970683
Produced by FastQC (version 0.11.5)